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Questions and Answers
What is the definition of partition coefficient in chromatography?
What is the definition of partition coefficient in chromatography?
What is the fundamental principle of chromatography?
What is the fundamental principle of chromatography?
What is the principle of adsorption chromatography?
What is the principle of adsorption chromatography?
What is the difference between adsorption chromatography and ion exchange chromatography?
What is the difference between adsorption chromatography and ion exchange chromatography?
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What is the principle of ion exchange chromatography?
What is the principle of ion exchange chromatography?
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Study Notes
Partition Coefficient in Chromatography
- Represents the ratio of the concentration of a compound in the stationary phase to its concentration in the mobile phase.
- Essential for understanding how substances distribute between two phases during separation.
- A higher partition coefficient indicates greater affinity for the stationary phase.
Fundamental Principle of Chromatography
- Based on the differential distribution of compounds between two immiscible phases: stationary and mobile.
- Allows the separation of components based on their affinities to the phases, which affects their velocities in the system.
Principle of Adsorption Chromatography
- Involves the adherence of solute molecules to the surface of solid stationary phase.
- Separation occurs due to variability in the adsorption strength of different solutes, influencing their movement through the column.
- Commonly used for polar compounds with varying degrees of adsorption.
Differences between Adsorption Chromatography and Ion Exchange Chromatography
- Adsorption chromatography focuses on the surface interactions of solutes with the stationary phase, while ion exchange chromatography is based on electrostatic interactions between charged ions and the oppositely charged stationary phase.
- Adsorption chromatography usually separates based on hydrophobicity and polarity, whereas ion exchange chromatography separates based on charge and ionic strength.
Principle of Ion Exchange Chromatography
- Operates by exchanging ions between the stationary phase and the mobile phase.
- Utilizes a charged resin to separate ions in a sample based on their affinity for the resin.
- Effective for purifying and concentrating ionic species, including proteins and small molecules.
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Description
Test your knowledge of chromatography with this quiz on the fundamental principles of the technique. Explore the processes of adsorption and partition and their significance in chromatographic analysis.
