10 Questions
In linear chromatography, what type of peak is produced if the separation obeys the distribution constant equation?
Gaussian peak
What is the equation for the distribution constant (K) in linear chromatography?
K = CS/CM
What does tM represent in chromatography?
Retention time of the mobile phase (dead time)
What is the average linear velocity of solute (V') expressed as?
V' = L / tR
What does U represent in the context of linear chromatography?
Linear velocity of the mobile phase
What contributes to zone broadening in chromatography?
Resistance to mass transfer, longitudinal diffusion, and multiple path effect
What does the equation $HL = \frac{L}{V} DM$ represent in chromatography?
Band broadening as a result of longitudinal diffusion
When does longitudinal diffusion significantly contribute to band broadening in liquid chromatography?
At low flow rates
What is the main factor contributing to zone broadening in mass transfer diffusion?
Resistance to mass transfer
What parameters affect the degree of band broadening in longitudinal diffusion?
Diffusion of the solute and the flow rate of the solute through the column
Learn about the distribution constant (K) in chromatography and how it is used to calculate the concentration of solutes in both stationary and mobile phases. Understand the conditions under which the separation is called linear chromatography.
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