18 Questions
What is the function of a chromatogram?
To show the detector response as a function of elution
What does the retention time (tr) represent in chromatography?
Time between sample injection and detector signal arrival
Which factor increases with longer retention of a solute in chromatography?
Retention factor
What does resolution measure in chromatography?
Separation between two peaks of different retention times
In chromatography, what does the separation factor (α) indicate?
The ratio of adjusted retention times of two components
What broadens as it moves through a chromatography column?
Band of solute
Which type of chromatography separates molecules by size, with larger solutes passing through most quickly?
Molecular exclusion chromatography
In which type of chromatography is the solute equilibrating between stationary liquid and flowing gas?
Ion-exchange chromatography
What is the main principle behind affinity chromatography?
Specific interactions between solute and immobilized molecules
Which type of chromatography is also known as size exclusion or gel filtration chromatography?
Molecular exclusion chromatography
In which type of chromatography are anions or cations covalently attached to the stationary solid phase?
Ion-exchange chromatography
Which type of chromatography employs specific interactions between solute molecules and a second molecule immobilized to the stationary phase?
Affinity chromatography
In adsorption chromatography, what is the role of the stationary phase?
To stay fixed inside the column
Which type of chromatography uses a liquid stationary phase bonded to a solid surface?
Partition chromatography
What happens if a solute is strongly adsorbed in adsorption chromatography?
It moves slower through the column
What is the mobile phase in partition chromatography?
Solvent moving through the column
Which type of chromatography involves a solid stationary phase and a liquid or gaseous mobile phase?
Adsorption chromatography
What is elution in chromatography?
The process of washing out a compound through a column using a solvent
Test your knowledge of chromatography basics with this quiz covering concepts such as sample injection, column, detector, retention time, retention factor, and chromatogram interpretation.
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