Podcast
Questions and Answers
What is the function of a chromatogram?
What is the function of a chromatogram?
- To inject the sample onto the column
- To read out the mobile phase
- To detect the signal from the detector
- To show the detector response as a function of elution (correct)
What does the retention time (tr) represent in chromatography?
What does the retention time (tr) represent in chromatography?
- Time taken for the sample to dissolve in the mobile phase
- Time between sample injection and detector signal arrival (correct)
- Time required to travel the length of the column by the solvent
- Time for band broadening during elution
Which factor increases with longer retention of a solute in chromatography?
Which factor increases with longer retention of a solute in chromatography?
- Retention factor (correct)
- Resolution
- Band broadening
- Relative retention
What does resolution measure in chromatography?
What does resolution measure in chromatography?
In chromatography, what does the separation factor (α) indicate?
In chromatography, what does the separation factor (α) indicate?
What broadens as it moves through a chromatography column?
What broadens as it moves through a chromatography column?
Which type of chromatography separates molecules by size, with larger solutes passing through most quickly?
Which type of chromatography separates molecules by size, with larger solutes passing through most quickly?
In which type of chromatography is the solute equilibrating between stationary liquid and flowing gas?
In which type of chromatography is the solute equilibrating between stationary liquid and flowing gas?
What is the main principle behind affinity chromatography?
What is the main principle behind affinity chromatography?
Which type of chromatography is also known as size exclusion or gel filtration chromatography?
Which type of chromatography is also known as size exclusion or gel filtration chromatography?
In which type of chromatography are anions or cations covalently attached to the stationary solid phase?
In which type of chromatography are anions or cations covalently attached to the stationary solid phase?
Which type of chromatography employs specific interactions between solute molecules and a second molecule immobilized to the stationary phase?
Which type of chromatography employs specific interactions between solute molecules and a second molecule immobilized to the stationary phase?
In adsorption chromatography, what is the role of the stationary phase?
In adsorption chromatography, what is the role of the stationary phase?
Which type of chromatography uses a liquid stationary phase bonded to a solid surface?
Which type of chromatography uses a liquid stationary phase bonded to a solid surface?
What happens if a solute is strongly adsorbed in adsorption chromatography?
What happens if a solute is strongly adsorbed in adsorption chromatography?
What is the mobile phase in partition chromatography?
What is the mobile phase in partition chromatography?
Which type of chromatography involves a solid stationary phase and a liquid or gaseous mobile phase?
Which type of chromatography involves a solid stationary phase and a liquid or gaseous mobile phase?
What is elution in chromatography?
What is elution in chromatography?
