Chromatography Basics Quiz

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What is the function of a chromatogram?

To inject the sample onto the column

To read out the mobile phase

To detect the signal from the detector

To show the detector response as a function of elution (correct)

What does the retention time (tr) represent in chromatography?

Time taken for the sample to dissolve in the mobile phase

Time between sample injection and detector signal arrival (correct)

Time required to travel the length of the column by the solvent

Time for band broadening during elution

Which factor increases with longer retention of a solute in chromatography?

Retention factor (correct)

Resolution

Band broadening

Relative retention

What does resolution measure in chromatography?

Separation between two peaks of different retention times Signup and view all the answers

In chromatography, what does the separation factor (α) indicate?

The ratio of adjusted retention times of two components Signup and view all the answers

What broadens as it moves through a chromatography column?

Band of solute Signup and view all the answers

Which type of chromatography separates molecules by size, with larger solutes passing through most quickly?

Molecular exclusion chromatography Signup and view all the answers

In which type of chromatography is the solute equilibrating between stationary liquid and flowing gas?

Ion-exchange chromatography Signup and view all the answers

What is the main principle behind affinity chromatography?

Specific interactions between solute and immobilized molecules Signup and view all the answers

Which type of chromatography is also known as size exclusion or gel filtration chromatography?

Molecular exclusion chromatography Signup and view all the answers

In which type of chromatography are anions or cations covalently attached to the stationary solid phase?

Ion-exchange chromatography Signup and view all the answers

Which type of chromatography employs specific interactions between solute molecules and a second molecule immobilized to the stationary phase?

Affinity chromatography Signup and view all the answers

In adsorption chromatography, what is the role of the stationary phase?

To stay fixed inside the column Signup and view all the answers

Which type of chromatography uses a liquid stationary phase bonded to a solid surface?

Partition chromatography Signup and view all the answers

What happens if a solute is strongly adsorbed in adsorption chromatography?

It moves slower through the column Signup and view all the answers

What is the mobile phase in partition chromatography?

Solvent moving through the column Signup and view all the answers

Which type of chromatography involves a solid stationary phase and a liquid or gaseous mobile phase?

Adsorption chromatography Signup and view all the answers

What is elution in chromatography?

The process of washing out a compound through a column using a solvent Signup and view all the answers

Chromatography Basics Quiz

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