Podcast
Questions and Answers
What is the main focus of Class A glassware?
What is the main focus of Class A glassware?
- General use without precision
- Resistance to heat and chemicals
- Lower cost and high volume
- High accuracy and tolerance (correct)
Which type of glass is characterized by excellent optical qualities and high cost?
Which type of glass is characterized by excellent optical qualities and high cost?
- Quartz/silica glass (correct)
- Polycarbonate glass
- Borosilicate glass
- Soda-lime glass
Which of the following glassware is made to NIST standards?
Which of the following glassware is made to NIST standards?
- Graduated cylinder (correct)
- Beaker
- Erlenmeyer flask
- Soda-lime flask
What is the primary use of a volumetric flask?
What is the primary use of a volumetric flask?
Which characteristic differentiates Class B from Class A glassware?
Which characteristic differentiates Class B from Class A glassware?
Which glass type is primarily used in laboratory settings for basic tasks such as mixing?
Which glass type is primarily used in laboratory settings for basic tasks such as mixing?
What is the approximate tolerance for Class B glassware?
What is the approximate tolerance for Class B glassware?
Which glassware is best for critical measurements in analytical chemistry?
Which glassware is best for critical measurements in analytical chemistry?
What is the primary purpose of a fume hood in a laboratory?
What is the primary purpose of a fume hood in a laboratory?
Which pipette type is calibrated to the tip?
Which pipette type is calibrated to the tip?
How often should the eye wash station be checked for maintenance?
How often should the eye wash station be checked for maintenance?
What should be done if chemical splashes into the eye?
What should be done if chemical splashes into the eye?
What is a critical safety instruction for using the fume hood?
What is a critical safety instruction for using the fume hood?
Which piece of equipment is primarily used to measure pH levels in solutions?
Which piece of equipment is primarily used to measure pH levels in solutions?
What is the main reason for performing daily and periodic inspections of the fume hood?
What is the main reason for performing daily and periodic inspections of the fume hood?
Which pipette type is not calibrated to the tip?
Which pipette type is not calibrated to the tip?
What is the primary use of an Erlenmeyer flask?
What is the primary use of an Erlenmeyer flask?
Which type of pipette is designed to deliver a fixed volume with high accuracy?
Which type of pipette is designed to deliver a fixed volume with high accuracy?
Which option best describes the calibration of a graduated cylinder?
Which option best describes the calibration of a graduated cylinder?
What is a common issue associated with laboratory plasticware?
What is a common issue associated with laboratory plasticware?
Which pipette type has graduations that extend to the tip?
Which pipette type has graduations that extend to the tip?
Which statement is true regarding the features of a Florence (Boiling) flask?
Which statement is true regarding the features of a Florence (Boiling) flask?
Which type of plastic is known for being clear and brittle, commonly used for disposable items?
Which type of plastic is known for being clear and brittle, commonly used for disposable items?
What distinguishes a Mohr pipette from other pipette types?
What distinguishes a Mohr pipette from other pipette types?
What occurs if a Mohr pipette is drained beyond the last calibration mark?
What occurs if a Mohr pipette is drained beyond the last calibration mark?
What is the precision of estimating volume when using a graduated cylinder marked with lines at every 10 mL and the smallest division of 1 mL?
What is the precision of estimating volume when using a graduated cylinder marked with lines at every 10 mL and the smallest division of 1 mL?
Which of the following is considered safe practice when handling glassware in the lab?
Which of the following is considered safe practice when handling glassware in the lab?
How should one insert glass tubing into a stopper for safety?
How should one insert glass tubing into a stopper for safety?
What is the correct procedure for dealing with cuts in the lab?
What is the correct procedure for dealing with cuts in the lab?
In what situation is it acceptable to use mouth pipetting?
In what situation is it acceptable to use mouth pipetting?
What type of precision does using a graduated cylinder for volume measurement provide?
What type of precision does using a graduated cylinder for volume measurement provide?
Which of the following is NOT an example of glassware mentioned for volume measurement?
Which of the following is NOT an example of glassware mentioned for volume measurement?
What does the process of tare refer to when weighing substances?
What does the process of tare refer to when weighing substances?
Which calibration method involves using external standard weights?
Which calibration method involves using external standard weights?
How often is daily calibration recommended for balances before laboratory work?
How often is daily calibration recommended for balances before laboratory work?
What is the primary purpose of using micropipettes in the lab?
What is the primary purpose of using micropipettes in the lab?
Which feature of a micropipette allows the user to set the desired volume?
Which feature of a micropipette allows the user to set the desired volume?
Why is it important to avoid spills on the weighing pan of a balance?
Why is it important to avoid spills on the weighing pan of a balance?
What is the maximum volume range of a P100 micropipette?
What is the maximum volume range of a P100 micropipette?
Which of the following describes the tolerance in the context of balances?
Which of the following describes the tolerance in the context of balances?
Study Notes
Safety Equipment
- Fume hood: Protects against inhaling hazardous chemicals; should not be used for storing explosive reactions or chemicals.
- Eye wash station: Used for rinsing eyes when chemicals are splashed in them.
- Safety shower: Used for large chemical spills on a person.
Glassware
- Soda-lime glass: The most inexpensive, low heat resistant. Commonly used for beakers, test tubes, and Erlenmeyer flasks.
- Borosilicate glass: Non-reactive, high thermal resistance. Used for beakers, volumetric flasks, and graduated cylinders.
- Quartz/Silica glass: Excellent optical qualities, expensive. Used for spectrophotometry cuvettes and optical lenses.
- Class A glassware: Made according to NIST standards; high accuracy and tolerance, used for volumetric flasks and graduated cylinders.
- Class B glassware: Less accurate than Class A; made from soda-lime glass, used for Erlenmeyer flasks and beakers.
Types of Glassware
- Beaker: Useful for estimating volumes, primarily used for holding and mixing liquids.
- Volumetric flask: Calibrated to contain (TC) a specific volume with high accuracy, used for preparing standard solutions.
- Erlenmeyer flask: More accurate than beakers for estimating volumes, used for mixing solutions.
- Florence (Boiling) flask: Used for heating liquids and observing reactions, primarily in chemistry/biology labs.
- Graduated cylinder: Used for measuring urine volumes and volumes of solutes/solvents for solution preparation.
- Test tubes: Used for holding small volumes of liquids.
Laboratory Plasticware
- Polyethylene: Clear or opaque; commonly used for disposable test tubes and graduated cylinders.
- Polypropylene: More heat-resistant, opaque; used for test tube racks.
- Polystyrene: Clear, brittle; used for disposable items like petri dishes and test tubes.
Pipettes
- Volumetric pipette: Class A, fixed volume delivery, used for precise measurements.
- Serological pipette: Graduations extend to the tip; variable volume, calibrated to deliver, used for serial dilutions and viscous liquids.
- Mohr pipette: Calibration marks do not reach the tip; variable volume, less accurate than volumetric.
Pipetting Safety
- Mouth pipetting is prohibited.
- Use a propipette or pipetting bulb.
Balances
- Top loading balance: less precise, commonly used to quickly measure large volumes.
- Analytical balance: highly precise, used to measure small amounts accurately.
- Tare: Removes the weight of a known object, ensuring measurement of only substance being weighed.
- Tolerance: Allowable degree of variation.
- Calibration: Ensures consistent and accurate weighing results, recommended daily.
- External calibration: Verifies accuracy using external standard weights.
- Internal calibration: Automatic calibration using built-in weights.
Micropipettes
- Can deliver fixed or variable volumes. Uses disposable tips.
- Calibrated to contain the stated volume (TC and TD).
- Common sizes: P2 (0.2-2 µL), P10 (1-10 µL), P20 (2-20 µL), P100 (20-100 µL), P200 (20-200 µL), P1000 (100-1000 µL).
- Parts: Plunger button, tip ejector, volume adjustment knob, micrometer, volume indicator, body, shaft, tip.
Importance of Pipette Calibration
- Maintains accuracy and precision.
- Ensures compliance with lab accreditation and quality of testing results.
- Types covered: Volumetric, serological, and Mohr pipettes.
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Description
Test your knowledge about essential safety equipment and various types of glassware used in the chemistry lab. This quiz covers the functions of safety gear as well as the characteristics of different glassware types, from soda-lime to borosilicate glass. Perfect for students preparing for lab work or just wanting to refresh their chemistry knowledge.
