Podcast
Questions and Answers
What type of lamps are used in UV-Visible spectrophotometry?
What type of lamps are used in UV-Visible spectrophotometry?
- Deuterium and tungsten filament (correct)
- Mercury and xenon
- Fluorescent and incandescent
- Halogen and LED
In which wavelength range does colorimetry mainly work?
In which wavelength range does colorimetry mainly work?
- 200-800 nm
- 400-700 nm (correct)
- 100-500 nm
- 700-1000 nm
What is the purpose of a monochromator in a UV-Visible spectrophotometer?
What is the purpose of a monochromator in a UV-Visible spectrophotometer?
- To analyze solid samples
- To emit visible light
- To separate polychromatic light into monochromatic light (correct)
- To generate UV light
Why are colorimeters considered cheap and easy to operate?
Why are colorimeters considered cheap and easy to operate?
Which region of the electromagnetic spectrum do UV-visible spectrophotometry and colorimetry cover?
Which region of the electromagnetic spectrum do UV-visible spectrophotometry and colorimetry cover?
Why are UV-Visible spectrometers more expensive compared to colorimeters?
Why are UV-Visible spectrometers more expensive compared to colorimeters?
What is the wavelength of maximum absorption known as?
What is the wavelength of maximum absorption known as?
In the UV-visible spectrum of lycopene, what is the lambda max value?
In the UV-visible spectrum of lycopene, what is the lambda max value?
What does the detector in a UV/VIS spectrophotometer or colorimeter measure?
What does the detector in a UV/VIS spectrophotometer or colorimeter measure?
What is the ratio between the intensities I / I0 defined as?
What is the ratio between the intensities I / I0 defined as?
Which law is applied for quantification based on the absorptivity of pure compounds?
Which law is applied for quantification based on the absorptivity of pure compounds?
What term refers to the attenuation of transmitted light by sample molecules in solution?
What term refers to the attenuation of transmitted light by sample molecules in solution?
What can happen if the molecules in a solution are at high concentrations?
What can happen if the molecules in a solution are at high concentrations?
Why is it essential for the solution not to contain molecules that emit fluorescence when excited?
Why is it essential for the solution not to contain molecules that emit fluorescence when excited?
What is necessary for determining the concentration of an unknown sample using UV/VIS spectroscopy?
What is necessary for determining the concentration of an unknown sample using UV/VIS spectroscopy?
How is a calibration line obtained for UV/VIS spectroscopy analysis?
How is a calibration line obtained for UV/VIS spectroscopy analysis?
What does the Lambert-Beer Law form the basis for in UV/VIS spectroscopy?
What does the Lambert-Beer Law form the basis for in UV/VIS spectroscopy?
What purpose do UV/VIS spectra serve in identifying components in a sample solution?
What purpose do UV/VIS spectra serve in identifying components in a sample solution?
What allows specific compounds to be identified in UV/VIS spectroscopy?
What allows specific compounds to be identified in UV/VIS spectroscopy?
How is the blank in UV/VIS spectroscopy typically measured?
How is the blank in UV/VIS spectroscopy typically measured?
What is the purpose of measuring the absorbance value of a peak in UV/VIS spectroscopy?
What is the purpose of measuring the absorbance value of a peak in UV/VIS spectroscopy?
Why is a blank measurement necessary in UV/VIS spectroscopy?
Why is a blank measurement necessary in UV/VIS spectroscopy?
In which market segments is UV/VIS spectroscopy commonly used as a quantitative analytical technique?
In which market segments is UV/VIS spectroscopy commonly used as a quantitative analytical technique?
What is added to a cuvette for blank measurement in UV/VIS spectroscopy?
What is added to a cuvette for blank measurement in UV/VIS spectroscopy?
How is absorbance calculated in a spectrophotometer?
How is absorbance calculated in a spectrophotometer?
What is the purpose of using absorbance values for standard solutions in spectrophotometry?
What is the purpose of using absorbance values for standard solutions in spectrophotometry?
Why is it important to measure absorbance in a spectrophotometer?
Why is it important to measure absorbance in a spectrophotometer?
What role do standard solutions play in UV/VIS spectrophotometric analysis?
What role do standard solutions play in UV/VIS spectrophotometric analysis?
Why is it necessary to extrapolate concentrations from a calibration curve in spectrophotometry?
Why is it necessary to extrapolate concentrations from a calibration curve in spectrophotometry?
