Podcast
Questions and Answers
What is indicative of a transmembrane segment in a hydropathy plot?
What is indicative of a transmembrane segment in a hydropathy plot?
- A mix of hydrophilic and hydrophobic residues
- More than 20 residues of low hydropathy index
- Less than 10 residues of high hydropathy index
- More than 20 residues of high hydropathy index (correct)
What is the mean hydrophobicity threshold according to Kyte Doolittle for predicting a transmembrane helix?
What is the mean hydrophobicity threshold according to Kyte Doolittle for predicting a transmembrane helix?
- 0.5
- 2.0
- 3.0
- 1.4 (correct)
What is the approximate free energy cost per residue to disrupt hydrogen bonds in the membrane?
What is the approximate free energy cost per residue to disrupt hydrogen bonds in the membrane?
- ~1 kcal/mol
- ~6 kcal/mol
- ~2 kcal/mol
- ~4 kcal/mol (correct)
What is the primary factor that drives the formation of an α-helix in a transmembrane segment?
What is the primary factor that drives the formation of an α-helix in a transmembrane segment?
What is the role of the dielectric constant in membrane proteins?
What is the role of the dielectric constant in membrane proteins?
What happens to non-polar domains in membranes due to their low dielectric constant?
What happens to non-polar domains in membranes due to their low dielectric constant?
In the context of hydropathy plots, what is the purpose of using a 'sliding window' technique?
In the context of hydropathy plots, what is the purpose of using a 'sliding window' technique?
What is the main purpose of the Kyte-Doolittle & Engelman-Steitz-Goldman scales in hydrophobicity analysis?
What is the main purpose of the Kyte-Doolittle & Engelman-Steitz-Goldman scales in hydrophobicity analysis?
What does the hydropathy index reflect in the context of hydropathy plots?
What does the hydropathy index reflect in the context of hydropathy plots?
When averaging the hydropathy index for successive segments of a polypeptide chain, what are these segments known as?
When averaging the hydropathy index for successive segments of a polypeptide chain, what are these segments known as?
How is a 'window' defined in the context of hydropathy plots?
How is a 'window' defined in the context of hydropathy plots?
What information can be obtained by analyzing the hydropathy profile using a 'sliding window' technique?
What information can be obtained by analyzing the hydropathy profile using a 'sliding window' technique?
Which of the following statements about polytopic membrane proteins is correct?
Which of the following statements about polytopic membrane proteins is correct?
Which of the following scales is commonly used to generate hydropathy plots for predicting membrane protein topology?
Which of the following scales is commonly used to generate hydropathy plots for predicting membrane protein topology?
What is a key factor that contributes to the hydrophobicity of an amino acid residue?
What is a key factor that contributes to the hydrophobicity of an amino acid residue?
In the context of membrane protein topology prediction, what does the term 'hydropathy plot' refer to?
In the context of membrane protein topology prediction, what does the term 'hydropathy plot' refer to?
Which of the following statements about overexpression and purification of integral membrane proteins is correct?
Which of the following statements about overexpression and purification of integral membrane proteins is correct?
In the context of membrane protein topology prediction, what does an 'even number of transmembrane segments' typically imply?
In the context of membrane protein topology prediction, what does an 'even number of transmembrane segments' typically imply?
