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Questions and Answers
What is a specialized version of the general technique of equilibrium or density gradient centrifugation?
What is a specialized version of the general technique of equilibrium or density gradient centrifugation?
- Organic extraction purification
- Alkaline denaturation
- Ethidium bromide-Cesium chloride density gradient (correct)
- Column chromatography
At what density does DNA band in a CsCl density gradient during centrifugation?
At what density does DNA band in a CsCl density gradient during centrifugation?
- 2.00 g/cm3
- 1.70 g/cm3 (correct)
- 1.60 g/cm3
- 1.80 g/cm3
Which macromolecule floats at the top of the tube during density gradient centrifugation?
Which macromolecule floats at the top of the tube during density gradient centrifugation?
- Plasmid DNA
- Protein (correct)
- DNA
- RNA
How can supercoiled DNA be separated from non-supercoiled molecules using density gradient centrifugation?
How can supercoiled DNA be separated from non-supercoiled molecules using density gradient centrifugation?
If the alkaline denaturation method is employed, is further purification by organic extraction or column chromatography necessary?
If the alkaline denaturation method is employed, is further purification by organic extraction or column chromatography necessary?
What is used to create a density gradient in density gradient centrifugation?
What is used to create a density gradient in density gradient centrifugation?
What is the primary mechanism by which ethidium bromide binds to DNA?
What is the primary mechanism by which ethidium bromide binds to DNA?
How does the binding of ethidium bromide affect the buoyant density of supercoiled DNA compared to linear DNA?
How does the binding of ethidium bromide affect the buoyant density of supercoiled DNA compared to linear DNA?
What is the purpose of using a CsCl gradient in the purification of plasmid DNA?
What is the purpose of using a CsCl gradient in the purification of plasmid DNA?
What is the purpose of using ultraviolet radiation in the plasmid DNA purification process?
What is the purpose of using ultraviolet radiation in the plasmid DNA purification process?
What is the primary advantage of using the CsCl-ethidium bromide gradient centrifugation method for plasmid DNA purification?
What is the primary advantage of using the CsCl-ethidium bromide gradient centrifugation method for plasmid DNA purification?
What is the purpose of the dialysis step in the plasmid DNA purification process?
What is the purpose of the dialysis step in the plasmid DNA purification process?
Which of the following statements about the cell disruption process is true?
Which of the following statements about the cell disruption process is true?
What is the primary reason for using a non-ionic detergent like Triton X-100 during cell lysis?
What is the primary reason for using a non-ionic detergent like Triton X-100 during cell lysis?
Why is centrifugation alone not sufficient for separating plasmid DNA from chromosomal DNA?
Why is centrifugation alone not sufficient for separating plasmid DNA from chromosomal DNA?
What is the primary advantage of using gentle cell lysis techniques?
What is the primary advantage of using gentle cell lysis techniques?
What is the purpose of using EDTA during the cell lysis process?
What is the purpose of using EDTA during the cell lysis process?
Which of the following statements about the centrifugation step is correct?
Which of the following statements about the centrifugation step is correct?
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