Podcast
Questions and Answers
What is the purpose of using dilute celloidin solutions in slide preparation?
What is the purpose of using dilute celloidin solutions in slide preparation?
- To provide a yellow color to collagen
- To remove stains from slides
- To improve visibility of nuclei
- To attach sections more firmly to the slide (correct)
What is the main source of the dye Hematoxylin?
What is the main source of the dye Hematoxylin?
- A synthetic chemical
- A root from an Asian tree
- The core of a Mexican tree (correct)
- A plant from South America
Hematoxylin is primarily used as a counterstain that produces blue nuclei.
Hematoxylin is primarily used as a counterstain that produces blue nuclei.
False (B)
What should be avoided during dehydration when using celloidin?
What should be avoided during dehydration when using celloidin?
Hematoxylin is classified as a true basic dye.
Hematoxylin is classified as a true basic dye.
The ______ of a log tree is the source of hematoxylin.
The ______ of a log tree is the source of hematoxylin.
What should be done if sections do not appear clear under the microscope after staining?
What should be done if sections do not appear clear under the microscope after staining?
Hematoxylin is formed by the oxidation of hematoxylin through a process known as ______.
Hematoxylin is formed by the oxidation of hematoxylin through a process known as ______.
What may cause failure of sections to stay on the slide during staining?
What may cause failure of sections to stay on the slide during staining?
Chloroform should be avoided when preparing slides for staining.
Chloroform should be avoided when preparing slides for staining.
Match the following types of stains with their characteristics:
Match the following types of stains with their characteristics:
What is a common issue that can cause failure of staining?
What is a common issue that can cause failure of staining?
What should be applied to the skin to effectively remove stains?
What should be applied to the skin to effectively remove stains?
Stains can only be used once and should be discarded after use.
Stains can only be used once and should be discarded after use.
Match the following staining substances with their effects:
Match the following staining substances with their effects:
How long can a slide be immersed in xylene for RESTAINING of old sections?
How long can a slide be immersed in xylene for RESTAINING of old sections?
What could happen if faults are not corrected during the processing of tissue?
What could happen if faults are not corrected during the processing of tissue?
Using a base-sledge microtome is recommended for effective decalcification of tissue.
Using a base-sledge microtome is recommended for effective decalcification of tissue.
What should a medical technologist be alert for during tissue processing?
What should a medical technologist be alert for during tissue processing?
The tilt of the knife should be reduced if the __________ of the block are not clear.
The tilt of the knife should be reduced if the __________ of the block are not clear.
Match each problem observed during cutting with its corresponding remedy:
Match each problem observed during cutting with its corresponding remedy:
Which of the following is NOT a potential issue during the cutting process?
Which of the following is NOT a potential issue during the cutting process?
What is the primary purpose of the Periodic Acid-Schiff (PAS) staining method?
What is the primary purpose of the Periodic Acid-Schiff (PAS) staining method?
Hematoxylin stains basic structures such as proteins and membranes.
Hematoxylin stains basic structures such as proteins and membranes.
Which stain is primarily used to highlight tissue and cells that contain iron?
Which stain is primarily used to highlight tissue and cells that contain iron?
The Nissl stain is specialized for __________ in neurons.
The Nissl stain is specialized for __________ in neurons.
Match the following types of stains with their main use:
Match the following types of stains with their main use:
What color does Eosin stain basic structures?
What color does Eosin stain basic structures?
Neutral dyes are formed by combining aqueous solutions of acid and basic dyes.
Neutral dyes are formed by combining aqueous solutions of acid and basic dyes.
What is the effect of immersing paraffin sections in a solvent prior to staining?
What is the effect of immersing paraffin sections in a solvent prior to staining?
Methylene blue is classified as a __________ dye.
Methylene blue is classified as a __________ dye.
Which of the following is NOT a benefit of using trichrome staining?
Which of the following is NOT a benefit of using trichrome staining?
What color does the Reticulin stain produce?
What color does the Reticulin stain produce?
Artifacts in histology only occur as a result of tissue processing.
Artifacts in histology only occur as a result of tissue processing.
What fungal infection is indicated in the image with superficial fungal elements?
What fungal infection is indicated in the image with superficial fungal elements?
A common example of pre-histology artifacts includes ___ from tattoos.
A common example of pre-histology artifacts includes ___ from tattoos.
Match the type of stain with its usage:
Match the type of stain with its usage:
Which of the following is NOT a post-histology artifact?
Which of the following is NOT a post-histology artifact?
Most post-histology artifacts can be avoided or removed after being discovered.
Most post-histology artifacts can be avoided or removed after being discovered.
What is the primary color used in Red Oil 3 staining?
What is the primary color used in Red Oil 3 staining?
Study Notes
Staining Techniques and Precautions
- Celloidin (cellulose nitrate) coats slides to secure tissue sections; it is soluble in absolute alcohol.
- Hematoxylin stains nuclei red and is primarily used as a counterstain and to highlight collagen as yellow.
- Precautions in staining involve avoiding health hazards from stains, ensuring slides are clean, and checking the quality of staining solutions.
Hematoxylin
- Derived from the heartwood of Hematoxylin Campechianum, it has strong nuclear and chromatin staining properties.
- Hematin, the active agent in hematoxylin staining, is formed by the oxidation process known as ripening.
- Sections can be restained multiple times if securely attached to the slide.
Synthetic and Acid Dyes
- Synthetic dyes, also called coal tar dyes or aniline dyes, consist of a chromophore and an auxochrome, derived from coal tar.
- Acid dyes contain the active coloring substance in their acid component; examples include picric acid and trichloroacetic acid.
- Basic dyes, such as methylene blue, have the active coloring in their basic component.
Staining Techniques
- The H & E (Hematoxylin and Eosin) stain is commonly used in histology, staining acidic structures blue and basic structures pink.
- TriChrome staining uses three dyes to distinctly highlight intracellular structures, enhancing visualization of blood cells and connective tissues.
Specific Stains
- Iron-Hematoxylin stain highlights tissues containing iron, such as muscle and red blood cells.
- Periodic Acid-Schiff (PAS) staining identifies glycogen and other polysaccharides by converting glycol groups into aldehyde groups, yielding a reddish-purple complex.
- Nissl stain targets rough endoplasmic reticulum in neurons, while silver staining highlights fibers and cytoskeletal elements.
Artifacts in Histology
- Pre-histology artifacts arise before tissue collection, such as tattoos or melanin in skin samples.
- Post-histology artifacts occur due to processing, causing structural changes and color alterations that may affect evaluation.
- Faults during cutting can lead to poor sectioning; common issues include tilt of the knife and improper trimming of the paraffin block.
Staining Process for Paraffin Sections
- Paraffin is poorly permeable; must be dissolved using solvent techniques before staining.
- Typical procedure involves immersion in solvents like xylene to remove paraffin, followed by various staining methods.
- Specific staining techniques such as silver staining for reticular fibers and the use of Romanowsky stains for blood cells highlight cellular details effectively.
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Description
This quiz explores the technique of using celluloidin solutions for preparing and staining slides in microscopy. It details the properties of cellulose nitrate and the importance of avoiding dehydration during the process. Test your understanding of collodionization and its applications in biological studies.
