Cellular Product Formulation Quiz

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What is the purpose of cryopreservation in cell manufacturing?

To reduce damage during freezing and thawing processes (correct)

To accelerate cell freezing

To enhance cell dehydration

To provide off-the-shelf product delivery

Which cooling rate is generally recommended for controlled rate freezing during cryopreservation?

-1 °C/min (correct)

-10 °C/min

+1 °C/min

+10 °C/min

What is the composition of HANK cells used in patient infusion?

Saline solution with 1% HSA (correct)

Saline solution with 6% HSA

Saline solution with 2% HSA

Saline solution with 5% HSA

What technical limitations are associated with cryopreservation?

Rapid cell dehydration during freezing Signup and view all the answers

How does cryopreservation impact the recovery of various cell types after thawing?

Depends on the cooling rate used during freezing Signup and view all the answers

Why is controlled rate freezing using commercial devices preferred in cryopreservation?

To minimize damage from rapid freezing Signup and view all the answers

What is a limitation of mRNA delivery via electroporation?

Results in only transient transgene expression Signup and view all the answers

Which viral vectors allow long-lasting stable transgene expression?

Lentiviral vectors Signup and view all the answers

What is a limitation of γ-retroviral vectors for gene delivery?

Dependence on cell proliferation Signup and view all the answers

How have third-generation lentiviral vectors improved safety for clinical purposes?

Reduced risk of replication-competent virus generation Signup and view all the answers

What is a feature of self-inactivating (SIN) lentiviral vectors that enhances safety?

3' long terminal repeats (LTR) deletions Signup and view all the answers

Which method has been reported in trials for engineering NK cells using transgene mRNA?

Electroporation Signup and view all the answers

What is the purpose of adding cryoprotectant agents to the product formulation?

To increase cell survival during freeze-thaw processes Signup and view all the answers

Why is dimethyl sulfoxide (DMSO) the most used cryoprotectant agent?

As it guarantees cell survival during freeze-thaw due to its properties Signup and view all the answers

Why should the pre-freeze and post-thaw time window be optimized when using DMSO as a cryoprotectant agent?

To minimize toxicity to cells Signup and view all the answers

What is a challenge associated with DMSO-free cryopreservation of T and NK cells?

It is still challenging due to toxicity concerns Signup and view all the answers

Which cryopreserved product formulation contains 20% HSA, 25% dextran-40, and 5% DMSO?

GC Pharma's MG4101 Signup and view all the answers

Why is post-thaw washing and CPA removal considered complex?

Because it involves a complex process to remove toxic agents from cells Signup and view all the answers