Cellular Product Formulation Quiz
18 Questions
1 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the purpose of cryopreservation in cell manufacturing?

  • To reduce damage during freezing and thawing processes (correct)
  • To accelerate cell freezing
  • To enhance cell dehydration
  • To provide off-the-shelf product delivery

    • Which cooling rate is generally recommended for controlled rate freezing during cryopreservation?

  • -1 °C/min (correct)
  • -10 °C/min
  • +1 °C/min
  • +10 °C/min

    • What is the composition of HANK cells used in patient infusion?

  • Saline solution with 1% HSA (correct)
  • Saline solution with 6% HSA
  • Saline solution with 2% HSA
  • Saline solution with 5% HSA

    • What technical limitations are associated with cryopreservation?

    <p>Rapid cell dehydration during freezing</p> Signup and view all the answers

    How does cryopreservation impact the recovery of various cell types after thawing?

    <p>Depends on the cooling rate used during freezing</p> Signup and view all the answers

    Why is controlled rate freezing using commercial devices preferred in cryopreservation?

    <p>To minimize damage from rapid freezing</p> Signup and view all the answers

    What is a limitation of mRNA delivery via electroporation?

    <p>Results in only transient transgene expression</p> Signup and view all the answers

    Which viral vectors allow long-lasting stable transgene expression?

    <p>Lentiviral vectors</p> Signup and view all the answers

    What is a limitation of γ-retroviral vectors for gene delivery?

    <p>Dependence on cell proliferation</p> Signup and view all the answers

    How have third-generation lentiviral vectors improved safety for clinical purposes?

    <p>Reduced risk of replication-competent virus generation</p> Signup and view all the answers

    What is a feature of self-inactivating (SIN) lentiviral vectors that enhances safety?

    <p>3' long terminal repeats (LTR) deletions</p> Signup and view all the answers

    Which method has been reported in trials for engineering NK cells using transgene mRNA?

    <p>Electroporation</p> Signup and view all the answers

    What is the purpose of adding cryoprotectant agents to the product formulation?

    <p>To increase cell survival during freeze-thaw processes</p> Signup and view all the answers

    Why is dimethyl sulfoxide (DMSO) the most used cryoprotectant agent?

    <p>As it guarantees cell survival during freeze-thaw due to its properties</p> Signup and view all the answers

    Why should the pre-freeze and post-thaw time window be optimized when using DMSO as a cryoprotectant agent?

    <p>To minimize toxicity to cells</p> Signup and view all the answers

    What is a challenge associated with DMSO-free cryopreservation of T and NK cells?

    <p>It is still challenging due to toxicity concerns</p> Signup and view all the answers

    Which cryopreserved product formulation contains 20% HSA, 25% dextran-40, and 5% DMSO?

    <p>GC Pharma's MG4101</p> Signup and view all the answers

    Why is post-thaw washing and CPA removal considered complex?

    <p>Because it involves a complex process to remove toxic agents from cells</p> Signup and view all the answers

    More Like This

    Use Quizgecko on...
    Browser
    Open
    Browser
    Browser