Podcast
Questions and Answers
What is the purpose of cryopreservation in cell manufacturing?
What is the purpose of cryopreservation in cell manufacturing?
- To reduce damage during freezing and thawing processes (correct)
- To accelerate cell freezing
- To enhance cell dehydration
- To provide off-the-shelf product delivery
Which cooling rate is generally recommended for controlled rate freezing during cryopreservation?
Which cooling rate is generally recommended for controlled rate freezing during cryopreservation?
- -1 °C/min (correct)
- -10 °C/min
- +1 °C/min
- +10 °C/min
What is the composition of HANK cells used in patient infusion?
What is the composition of HANK cells used in patient infusion?
- Saline solution with 1% HSA (correct)
- Saline solution with 6% HSA
- Saline solution with 2% HSA
- Saline solution with 5% HSA
What technical limitations are associated with cryopreservation?
What technical limitations are associated with cryopreservation?
How does cryopreservation impact the recovery of various cell types after thawing?
How does cryopreservation impact the recovery of various cell types after thawing?
Why is controlled rate freezing using commercial devices preferred in cryopreservation?
Why is controlled rate freezing using commercial devices preferred in cryopreservation?
What is a limitation of mRNA delivery via electroporation?
What is a limitation of mRNA delivery via electroporation?
Which viral vectors allow long-lasting stable transgene expression?
Which viral vectors allow long-lasting stable transgene expression?
What is a limitation of γ-retroviral vectors for gene delivery?
What is a limitation of γ-retroviral vectors for gene delivery?
How have third-generation lentiviral vectors improved safety for clinical purposes?
How have third-generation lentiviral vectors improved safety for clinical purposes?
What is a feature of self-inactivating (SIN) lentiviral vectors that enhances safety?
What is a feature of self-inactivating (SIN) lentiviral vectors that enhances safety?
Which method has been reported in trials for engineering NK cells using transgene mRNA?
Which method has been reported in trials for engineering NK cells using transgene mRNA?
What is the purpose of adding cryoprotectant agents to the product formulation?
What is the purpose of adding cryoprotectant agents to the product formulation?
Why is dimethyl sulfoxide (DMSO) the most used cryoprotectant agent?
Why is dimethyl sulfoxide (DMSO) the most used cryoprotectant agent?
Why should the pre-freeze and post-thaw time window be optimized when using DMSO as a cryoprotectant agent?
Why should the pre-freeze and post-thaw time window be optimized when using DMSO as a cryoprotectant agent?
What is a challenge associated with DMSO-free cryopreservation of T and NK cells?
What is a challenge associated with DMSO-free cryopreservation of T and NK cells?
Which cryopreserved product formulation contains 20% HSA, 25% dextran-40, and 5% DMSO?
Which cryopreserved product formulation contains 20% HSA, 25% dextran-40, and 5% DMSO?
Why is post-thaw washing and CPA removal considered complex?
Why is post-thaw washing and CPA removal considered complex?
