Ch.1 Cellular Biology
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Questions and Answers

What is the fundamental concept that underlies the understanding of disease?

The integrity of cells.

What is the term used to describe the process of how cells communicate with each other?

Cellular crosstalk

What is the purpose of intercellular signals in maintaining cellular function?

To allow cells to determine their position and specialized role.

What is the result when cells no longer demonstrate a 'chemical fondness' for other cells and their environment?

<p>The conversation breaks down, leading to cellular adaptation, isolation, injury, disease, or death.</p> Signup and view all the answers

How do cells maintain the integrity of the entire organism?

<p>By demonstrating a 'chemical fondness' for other cells and their surrounding environment.</p> Signup and view all the answers

What is the outcome when cells adapt to changes in their environment?

<p>They may alter their function.</p> Signup and view all the answers

What are the two major classes of living cells?

<p>Eukaryotes and prokaryotes</p> Signup and view all the answers

What characteristic feature do eukaryotic cells have that prokaryotes lack?

<p>A well-defined nucleus</p> Signup and view all the answers

What type of organism are fungi, protozoa, and most algae?

<p>Eukaryotes</p> Signup and view all the answers

What is the term for the membrane-bound compartments found in eukaryotic cells?

<p>Organelles</p> Signup and view all the answers

What type of chromosome do prokaryotic cells have?

<p>A single circular chromosome</p> Signup and view all the answers

What is the function of histones in eukaryotic cells?

<p>They bind with DNA and are involved in the supercoiling of DNA</p> Signup and view all the answers

How do protein synthesis mechanisms differ between prokaryotic and eukaryotic cells?

<p>Due to structural differences in RNA-protein complexes</p> Signup and view all the answers

What is a key difference in the mechanism of transport across the outer cellular membrane between prokaryotes and eukaryotes?

<p>Differences in mechanisms of transport</p> Signup and view all the answers

What is a distinction between prokaryotic and eukaryotic cells in terms of enzyme content?

<p>They have different enzyme contents</p> Signup and view all the answers

Why are eukaryotic cells generally larger than prokaryotic cells?

<p>Due to their more extensive intracellular anatomy and organization</p> Signup and view all the answers

What is the primary function of muscle cells?

<p>To generate forces that produce motion</p> Signup and view all the answers

What is the chief function of nerve cells?

<p>Conductivity, allowing a wave of excitation to pass along the surface of the cell</p> Signup and view all the answers

What is the specialized function of intestinal epithelial cells?

<p>To reabsorb fluids and synthesize protein enzymes</p> Signup and view all the answers

What is the main function of cells in the kidney tubules?

<p>To reabsorb fluids and synthesize proteins</p> Signup and view all the answers

What is the function of mucous gland cells?

<p>To synthesize and secrete new substances</p> Signup and view all the answers

How do cells eliminate waste products?

<p>Through membrane-bound sacs called lysosomes, which contain enzymes that break down large molecules</p> Signup and view all the answers

What is the primary function of cells in the adrenal gland, testis, and ovary?

<p>To secrete hormonal steroids</p> Signup and view all the answers

What is the main difference between specialized cells in terms of function?

<p>Some cells are specialized for movement, while others are specialized for functions like conductivity, metabolic absorption, secretion, or excretion</p> Signup and view all the answers

What is the outcome when cells become specialized?

<p>They lose some other properties, such as hormone production, in favor of a highly developed function</p> Signup and view all the answers

What is the common function shared by all cells?

<p>The elimination of waste products through excretion</p> Signup and view all the answers

What is the primary site of RNA processing?

<p>Nucleolus</p> Signup and view all the answers

What is the main site for protein synthesis and degradation?

<p>Cytosol</p> Signup and view all the answers

What is the term for the space between the nuclear envelope and the plasma membrane?

<p>Cytoplasmic matrix</p> Signup and view all the answers

What is the purpose of biologic membranes surrounding organelles?

<p>To enable simultaneous functions in different biochemical environments</p> Signup and view all the answers

What happens to newly synthesized proteins without a sorting signal?

<p>They remain in the cytosol</p> Signup and view all the answers

What is the function of the cytosol as a storage unit?

<p>To store fat, carbohydrate, and secretory vesicles</p> Signup and view all the answers

What is the role of RNA in directing cellular activities?

<p>To carry coded messages from the nucleus to direct cellular activities</p> Signup and view all the answers

What is the percentage of the cell volume occupied by the cytosol?

<p>About half</p> Signup and view all the answers

What is present in the cytosol involved in intermediate metabolism?

<p>Thousands of enzymes</p> Signup and view all the answers

What is the purpose of ribosomes in the cytosol?

<p>To make proteins</p> Signup and view all the answers

What is the outer membrane of a eukaryotic cell called?

<p>plasma membrane or plasmalemma</p> Signup and view all the answers

What is the function of the nuclear pore complexes (NPCs)?

<p>to allow molecules to move between the nucleus and the cytosol</p> Signup and view all the answers

What is the main function of histones in eukaryotic cells?

<p>to regulate DNA activity by binding to DNA</p> Signup and view all the answers

What is the primary function of the nucleus?

<p>cell division and control of genetic information</p> Signup and view all the answers

What is the significance of the nuclear envelope being continuous with the membranes of the endoplasmic reticulum?

<p>it allows for communication and exchange between the nucleus and the rest of the cell</p> Signup and view all the answers

What is the purpose of DNA folding into chromosomes in eukaryotic cells?

<p>to prevent DNA breakage and facilitate cell division</p> Signup and view all the answers

What is the role of the nucleolus in the nucleus?

<p>it is a small, dense structure composed largely of RNA</p> Signup and view all the answers

What is the result of the advances in microscopy and computer software?

<p>resolution to the nanoscale, allowing cells to seem 'alive' with the molecular world more visible</p> Signup and view all the answers

What is the significance of understanding the structure and function of eukaryotic cells?

<p>it will reveal how cells respond to mechanical forces and emerge from different gene expression patterns</p> Signup and view all the answers

What is the overall impact of understanding eukaryotic cells on biology?

<p>it is huge, with significant implications for our understanding of biological processes</p> Signup and view all the answers

What is the critical process that occurs in the endoplasmic reticulum (ER) that is vital for cellular function?

<p>protein folding</p> Signup and view all the answers

What is the term used to describe the cellular response to the accumulation of unfolded or misfolded proteins in the ER?

<p>unfolded-protein response (UPR)</p> Signup and view all the answers

What are the membranous structures that bud from the Golgi complex and contain proteins and lipids for secretion or transport to other organelles?

<p>secretory vesicles</p> Signup and view all the answers

What is the type of vesicle that has a coat made largely of the protein clathrin and is involved in the inward endocytic pathway?

<p>clathrin-coated vesicle</p> Signup and view all the answers

What is the organelle that communicates with the Golgi complex and is involved in protein synthesis, folding, and modification?

<p>endoplasmic reticulum (ER)</p> Signup and view all the answers

What is the process by which the ER responds to the accumulation of misfolded proteins and initiates a stress response?

<p>ER stress</p> Signup and view all the answers

What is the term used to describe the movement of proteins from the ER to the Golgi complex for further processing and modification?

<p>secretory pathway</p> Signup and view all the answers

What is the function of chaperones in the ER?

<p>facilitate protein folding and prevent off-pathway interactions</p> Signup and view all the answers

What is the consequence of misfolded proteins not being repaired in the ER?

<p>apoptosis or cell death</p> Signup and view all the answers

What is the significance of understanding the mechanisms of ER stress and the unfolded-protein response?

<p>diagnosis and treatment of disease</p> Signup and view all the answers

What is the main function of ribosomes?

<p>To provide sites for cellular protein synthesis</p> Signup and view all the answers

What is the role of signal recognition particles (SRPs) in protein synthesis?

<p>To bind to the ribosome after recognizing the signal sequence and direct the protein to the endoplasmic reticulum</p> Signup and view all the answers

What is the difference between rough and smooth endoplasmic reticulum?

<p>Rough ER has ribosomes attached to it, while smooth ER does not</p> Signup and view all the answers

What is the function of the endoplasmic reticulum?

<p>To synthesize and transport proteins and lipids</p> Signup and view all the answers

What is the significance of the nuclear envelope being continuous with the endoplasmic reticulum?

<p>It allows for the transport of proteins and lipids from the ER to the nucleus</p> Signup and view all the answers

What is the role of the nucleolus in the nucleus?

<p>To synthesize ribosomes</p> Signup and view all the answers

What is the function of the nuclear pore complexes (NPCs)?

<p>To allow the transport of molecules between the nucleus and cytoplasm</p> Signup and view all the answers

What is the result of the binding of signal recognition particles (SRPs) to the ribosome?

<p>The ribosome is directed to the endoplasmic reticulum</p> Signup and view all the answers

What is the fate of the newly synthesized protein after it is synthesized by the ribosome?

<p>It is threaded through the ER membrane into the lumen and folded into its final conformation</p> Signup and view all the answers

What is the significance of the endoplasmic reticulum in sensing cellular stress?

<p>It plays a new role in sensing cellular stress</p> Signup and view all the answers

What is the primary function of lysosomes in the cell?

<p>The primary function of lysosomes is to break down and recycle cellular waste and foreign substances through the process of digestion and degradation, serving as the cell's 'recycling centers'.</p> Signup and view all the answers

What type of enzymes are found in lysosomes?

<p>Hydrolases</p> Signup and view all the answers

At what pH do lysosomal enzymes function optimally?

<p>Acidic pH</p> Signup and view all the answers

What is the significance of lysosomes beyond their degradative role?

<p>Lysosomes play a central role in nutrient-dependent signal transduction for cellular adaptation.</p> Signup and view all the answers

What is the outcome of altered lysosomal function?

<p>Alterations in lysosomal function are implicated in a range of diseases, including storage diseases, neurodegenerative diseases, and cancer.</p> Signup and view all the answers

What is the role of transport proteins in the lysosome membrane?

<p>Transport proteins in the lysosome membrane carry the final breakdown products to the cytosol for reuse or excretion.</p> Signup and view all the answers

What is the result of efficient lysosomal function?

<p>Efficient removal of toxic cellular components, maintenance of metabolic homeostasis, and termination of signal transduction.</p> Signup and view all the answers

What is the relationship between lysosomes and cellular adaptation?

<p>Lysosomes play a central role in nutrient-dependent signal transduction for cellular adaptation.</p> Signup and view all the answers

What is the significance of lysosomal function in cellular maintenance?

<p>Lysosomes maintain cellular health by removing toxic cellular components, terminating signal transduction, and maintaining metabolic homeostasis.</p> Signup and view all the answers

How do lysosomes interact with other cellular organelles?

<p>Lysosomes interact with other organelles, such as the endoplasmic reticulum, to regulate cellular metabolism and adaptation.</p> Signup and view all the answers

What is the purpose of the lysosomal membrane?

<p>To act as a protective shield between the powerful digestive enzymes within the lysosome and the cytoplasm, preventing their leakage into the cytoplasmic matrix.</p> Signup and view all the answers

What happens when lysosomal enzymes leak into the cytoplasmic matrix?

<p>Cellular self-digestion occurs, which can lead to cellular injury and death.</p> Signup and view all the answers

What is the name of the disease caused by the lack of lysosomal α-1,4-glucosidase?

<p>Pompe disease</p> Signup and view all the answers

What is the name of the disease caused by the deficiency or absence of lysosomal hexosaminidase A?

<p>Tay-Sachs disease</p> Signup and view all the answers

What is the term for the uptake of macromolecules from extracellular fluid?

<p>Endocytosis</p> Signup and view all the answers

What is the term for the engulfment of large particles or microorganisms by phagocytic cells?

<p>Phagocytosis</p> Signup and view all the answers

What is the term for the self-digestion process that begins in the cytosol and is used to digest cytosol and ineffectual organelles?

<p>Autophagy</p> Signup and view all the answers

What is the method by which lysosomes maintain a low internal pH?

<p>By pumping hydrogen ions into their interiors</p> Signup and view all the answers

What is the result of undigested uric acid accumulation within lysosomes in gout?

<p>Damage to the lysosomal membrane, leading to enzyme leakage and cell death.</p> Signup and view all the answers

What is the term for the membrane-bound vesicle that forms when extracellular substances are taken into the cell?

<p>Digestive vacuole</p> Signup and view all the answers

What is the pH level at which hydrolytic enzymes are maximally active?

<p>Acidic pH values</p> Signup and view all the answers

What is the term for lysosomes that are not active and do not maintain an acidic internal pH?

<p>Primary lysosomes</p> Signup and view all the answers

What is the process called when lysosomes digest cellular debris or obsolete parts?

<p>Autophagy</p> Signup and view all the answers

What is the term for lysosomes involved in autophagy?

<p>Autolysosomes or autophagosomes</p> Signup and view all the answers

What is the fate of indigestible material in lysosomes?

<p>Stored in residual bodies</p> Signup and view all the answers

What is the term for the pigmented substance that accumulates in cells as a result of autophagy?

<p>Lipofuscin</p> Signup and view all the answers

What is the role of autophagy in cellular function?

<p>Promotes homeostasis and cell turnover</p> Signup and view all the answers

What happens to the products of autophagy?

<p>Passed out of the lysosome and reused by the cell</p> Signup and view all the answers

What is the outcome of defects in autophagy?

<p>Contribution to disease</p> Signup and view all the answers

When do lysosomes become active?

<p>When primary lysosomes fuse with a vacuole or other organelle</p> Signup and view all the answers

What is the primary function of peroxisomes?

<p>To detoxify compounds and fatty acids</p> Signup and view all the answers

What is the role of catalase in peroxisomes?

<p>To oxidize various substrates and break down hydrogen peroxide</p> Signup and view all the answers

What is the shape and size of peroxisomes compared to lysosomes?

<p>Larger and oval or irregular in shape</p> Signup and view all the answers

What is the major site of oxygen utilization in a cell?

<p>Peroxisomes and mitochondria</p> Signup and view all the answers

What is the function of mitochondria in a cell?

<p>Cellular respiration and energy production</p> Signup and view all the answers

What is the structure of the mitochondrial inner membrane?

<p>Convoluted to form partitions called cristae</p> Signup and view all the answers

What is the result of impairment of peroxisomes?

<p>Disease</p> Signup and view all the answers

What is the role of peroxisomes in the synthesis of specialized phospholipids?

<p>Necessary for nerve cell myelination</p> Signup and view all the answers

What is the function of the enzymes in the mitochondrial inner membrane?

<p>Essential for oxidative phosphorylation</p> Signup and view all the answers

What is the purpose of the cristae in the mitochondrial inner membrane?

<p>To increase the surface area for oxidative phosphorylation</p> Signup and view all the answers

What percentage of the total cell volume is accounted for by the cytosol?

<p>55%</p> Signup and view all the answers

What type of biochemical reactions occur in the cytosol?

<p>Enzymatic biochemical reactions</p> Signup and view all the answers

What is the purpose of ribosomal protein synthesis in the cytosol?

<p>To synthesize proteins</p> Signup and view all the answers

What is the term for the process of synthesizing, degrading, and transforming small organic molecules in the cytosol?

<p>Intermediary metabolism</p> Signup and view all the answers

What type of molecules are stored in the cytosol as glycogen?

<p>Excess glucose</p> Signup and view all the answers

What is the function of secretory vesicles in the cytosol?

<p>To transport and empty their contents outside the cell</p> Signup and view all the answers

What are the clusters of ribosomes in the cytosol called that synthesize identical proteins?

<p>Polyribosomes</p> Signup and view all the answers

What is the site of protein synthesis in the cytosol?

<p>Free ribosomes</p> Signup and view all the answers

What is the purpose of the cytosol in terms of cellular activities?

<p>To provide energy and substrates for maintaining cell integrity</p> Signup and view all the answers

What is the primary function of microtubules in the cell?

<p>Microtubules provide strength to the cell's structure, support and move organelles, facilitate transport of impulses along nerve cells, and have roles in inflammatory and immune responses and hormone secretion.</p> Signup and view all the answers

What is mechanotransduction, and what is its significance in cells?

<p>Mechanotransduction is the process by which cells translate mechanical stimuli into biochemical signals, allowing cells to adapt to their surroundings.</p> Signup and view all the answers

What is the function of cilia on cells lining the respiratory tract?

<p>To move substances, such as mucus, toward the throat, where it can be removed by coughing.</p> Signup and view all the answers

What are the three main types of filaments that make up the cytoskeleton?

<p>Actin filaments, microtubules, and intermediate filaments.</p> Signup and view all the answers

What is the arrangement of microtubules in a centriole?

<p>Nine bundles containing three microtubules each.</p> Signup and view all the answers

How do cells sense their physical environment?

<p>Through the cytoskeleton, which translates mechanical forces and deformations into biochemical signals.</p> Signup and view all the answers

Where are actin filaments usually concentrated in the cell?

<p>In the cell cortex, just beneath the plasma membrane.</p> Signup and view all the answers

What is the significance of the cytoskeleton in eukaryotic cells?

<p>The cytoskeleton maintains the cell's shape and internal organization, and permits movement of substances within the cell and movement of external projections.</p> Signup and view all the answers

What is the significance of intermediate filaments in epithelial tissue?

<p>They bridge the cytoplasm from one cell junction to another, supporting and strengthening the sheet of epithelium.</p> Signup and view all the answers

What is the role of microtubules in the external movement or motility of some cells?

<p>Microtubules are involved in the external movement or motility of some cells.</p> Signup and view all the answers

What is the term used to describe the arrangement of microfilaments in motile cells?

<p>A 'wave of excitation'.</p> Signup and view all the answers

What is the term used to describe the cellular processes that translate mechanical stimuli into biochemical signals?

<p>Mechanotransduction.</p> Signup and view all the answers

What is the function of microtubules during cellular division?

<p>To separate and migrate to opposite poles of the cell.</p> Signup and view all the answers

What is the significance of the cytoskeleton in maintaining cell shape and internal organization?

<p>The cytoskeleton provides the structural framework that maintains the cell's shape and internal organization.</p> Signup and view all the answers

What is the role of actin filaments in cell junctions?

<p>To link the interior of the cell to adjacent cells.</p> Signup and view all the answers

What are the consequences of cell stresses that involve adaptations of mechanotransduction?

<p>Cell stresses that involve adaptations of mechanotransduction are associated with several alterations and diseases, including loss of hearing, cardiovascular disease, muscular dystrophy, and cancer.</p> Signup and view all the answers

What are the three main components of the cytoskeleton?

<p>Actin filaments, microtubules, and intermediate filaments.</p> Signup and view all the answers

What is the significance of the nuclear lamina?

<p>It creates a protective chamber for the cell's DNA.</p> Signup and view all the answers

What is the function of microtubules in flagella?

<p>To enable the movement of sperm cells.</p> Signup and view all the answers

What is the main component of the cortical actin network?

<p>Actin filaments.</p> Signup and view all the answers

What is the main function of phospholipids in cell membranes?

<p>repairing the membrane</p> Signup and view all the answers

What is the term for the temporary, dynamic formations of domains in the plasma membrane?

<p>lipid rafts</p> Signup and view all the answers

What is the function of inositol phospholipids in cell signaling?

<p>responding to extracellular signals</p> Signup and view all the answers

What is the purpose of the hydrophilic region of a lipid molecule?

<p>immerse in water</p> Signup and view all the answers

What is the result of the bilayer structure of the plasma membrane?

<p>it is impermeable to most water-soluble molecules</p> Signup and view all the answers

What is the function of caveolae in cell communication?

<p>important for endocytosis</p> Signup and view all the answers

What is the main component of cell membranes in animals?

<p>phospholipids</p> Signup and view all the answers

What is the characteristic of lipid molecules that allows them to form a bilayer?

<p>amphipathic</p> Signup and view all the answers

What is the result of the fluidity of the plasma membrane?

<p>components of the cellular environment move slowly and selectively across the membrane</p> Signup and view all the answers

What is the function of lipids along with protein assemblies in the plasma membrane?

<p>acting as 'molecular glue' for the structural integrity of the membrane</p> Signup and view all the answers

What is the main function of cell membranes in controlling the composition of the space they enclose?

<p>They can allow or exclude various molecules, and move molecules in or out of the space through selective transport systems.</p> Signup and view all the answers

How do polarized domains contribute to directional transport across cell membranes?

<p>They facilitate directional transport by creating distinct apical and basolateral domains.</p> Signup and view all the answers

What is the role of protein receptors in cell-to-cell recognition?

<p>They act as markers that identify a cell to its neighbors.</p> Signup and view all the answers

What is the basic structure of cell membranes?

<p>The lipid bilayer, composed of two continuous opposing leaflets and proteins that span the bilayer or interact with the lipids on either side of the two leaflets.</p> Signup and view all the answers

What is the function of membrane proteins in cell membranes?

<p>They mediate most of the functions of the membrane, including transport of molecules across the membrane and ATP synthesis.</p> Signup and view all the answers

What is the significance of cell polarity in maintaining normal cell and tissue structure?

<p>It maintains normal cell and tissue structure for numerous functions, most importantly transport of nutrients in and out of the cell.</p> Signup and view all the answers

What is the role of the plasma membrane in cellular mobility and maintenance of cellular shape?

<p>It assists with cellular mobility and maintenance of cellular shape.</p> Signup and view all the answers

What is the significance of the lipid bilayer in cell membranes?

<p>It provides the basic fluid structure of the membrane and is mostly an impermeable barrier to water-soluble molecules.</p> Signup and view all the answers

How do individual lipid molecules move within the lipid bilayer?

<p>They can diffuse readily throughout their own monolayer.</p> Signup and view all the answers

What is the overall function of cell membranes in controlling metabolic pathways?

<p>They exert a powerful influence on metabolic pathways by controlling the movement of substances from one compartment to another.</p> Signup and view all the answers

What is the primary function of proteins in facilitating transport across membranes?

<p>Proteins act as receptors, enzymes, or transporters, serving as recognition and binding units, pores or transport channels, specific enzymes, cell surface markers, cell adhesion molecules, and catalysts of chemical reactions.</p> Signup and view all the answers

What is the state of cell balance referred to as in terms of protein synthesis, folding, and degradation?

<p>Proteostasis</p> Signup and view all the answers

What is the function of molecular chaperones called heat-shock proteins (hsp) in protein homeostasis?

<p>They help refold misfolded proteins, facilitate transportation, and ubiquitinate proteins for degradation.</p> Signup and view all the answers

What is the outcome when cells lack a sorting signal for protein transport?

<p>Proteins remain in the cytosol permanently.</p> Signup and view all the answers

What is the role of vesicles in protein transport?

<p>Vesicles discharge their cargo into a different compartment by fusing with that compartment's membrane.</p> Signup and view all the answers

What is the significance of understanding protein trafficking and misfolded proteins in disease?

<p>Misfolded proteins can cause disease, and understanding protein trafficking and misfolded proteins can provide insights into disease mechanisms.</p> Signup and view all the answers

What is the function of the proteostasis network in regulating protein homeostasis?

<p>The proteostasis network regulates protein homeostasis under various conditions, including nutrient supply variation, oxidative stress, cellular differentiation, temperature changes, and heavy metal ion presence.</p> Signup and view all the answers

What is the role of proteins in maintaining cellular shape and adhesion?

<p>Proteins act as cell adhesion molecules (CAMs), allowing cells to hook together and form attachments to the cytoskeleton.</p> Signup and view all the answers

What is the outcome when the proteostasis network fails or malfunctions?

<p>It is associated with human disease.</p> Signup and view all the answers

What is the role of proteins in energy transduction across the plasma membrane?

<p>Membrane proteins are key components of energy transduction, converting chemical energy into electrical energy or mechanical energy.</p> Signup and view all the answers

How do membrane proteins associate with the lipid bilayer?

<p>Membrane proteins associate with the lipid bilayer in different ways, including transmembrane proteins, proteins located almost entirely in the cytosol, proteins existing outside the bilayer, and proteins bound indirectly to one or the other bilayer membrane face.</p> Signup and view all the answers

What is the significance of the amino acid sequence in protein synthesis?

<p>The amino acid sequence contains sorting signals that direct the delivery of proteins to locations outside the cytosol or to organelle surfaces.</p> Signup and view all the answers

What are the three ways proteins move from one compartment to another?

<p>Gated transport, protein translocation, and vesicular transport.</p> Signup and view all the answers

What is the function of nuclear pore complexes in gated transport?

<p>Nuclear pore complexes direct the movement of proteins and RNA molecules between the cytosol and the nucleus.</p> Signup and view all the answers

What is the characteristic feature of membrane proteins?

<p>Membrane proteins are amphiphilic, with both hydrophobic and hydrophilic regions.</p> Signup and view all the answers

How do proteins fold into their functional configurations?

<p>Proteins fold into densely folded molecular configurations, with an excess of hydrophilic units at the surface and an excess of hydrophobic units inside.</p> Signup and view all the answers

What is the role of ribosomes in protein synthesis?

<p>Ribosomes synthesize proteins mainly in the cytosol.</p> Signup and view all the answers

What is the significance of the unique sequence of amino acids in proteins?

<p>The unique sequence of amino acids determines the specific function of each protein.</p> Signup and view all the answers

What is the function of the cytosol in protein synthesis?

<p>The cytosol is the site where proteins are synthesized and trafficked to their destinations.</p> Signup and view all the answers

What is the significance of the interaction between proteins and the lipid bilayer?

<p>The interaction between proteins and the lipid bilayer is crucial for membrane protein function and cell signaling.</p> Signup and view all the answers

What is the primary function of proteases in the human body?

<p>Breakdown of proteins.</p> Signup and view all the answers

What is the term used to describe the carbohydrate coating on the outside of the plasma membrane?

<p>Glycocalyx (or cell coat).</p> Signup and view all the answers

What are the four major proteolytic cascades with disease relevance?

<p>Cell death or caspase-mediated apoptosis, the blood coagulation cascade, degrading membrane enzymes or matrix metalloproteinase cascade, and the complement cascade.</p> Signup and view all the answers

What is the function of the carbohydrate coating on the cell surface?

<p>Protection from mechanical damage and assistance in cell mobility.</p> Signup and view all the answers

What is the role of glycoproteins, proteoglycans, and glycolipids in the cell membrane?

<p>They contain short chains of sugars or carbohydrates (oligosaccharides) that are involved in cell-cell recognition and adhesion.</p> Signup and view all the answers

What is the significance of dysregulation of proteases in human diseases?

<p>It features prominently in many human diseases, including cancer, autoimmunity, and neurodegenerative disorders.</p> Signup and view all the answers

What is the purpose of the glycocalyx in terms of cell-cell interaction?

<p>It participates in specific cell-cell recognition and adhesion.</p> Signup and view all the answers

What is the function of proteases in the proteolytic cascade?

<p>They participate in a tightly orchestrated sequence of events, acting as initiators, amplifiers, and executors.</p> Signup and view all the answers

What is the outcome of the dysregulation of proteases in the proteolytic cascade?

<p>It leads to many human diseases, including cancer, autoimmunity, and neurodegenerative disorders.</p> Signup and view all the answers

What is the significance of the glycocalyx in terms of cell protection?

<p>It helps protect the cell from mechanical damage.</p> Signup and view all the answers

What is the function of lectins in recognizing neutrophils at the site of bacterial infection?

<p>Lectins recognize and bind to specific oligosaccharides on neutrophils, allowing them to adhere to the blood vessel wall and migrate to the infected tissue to eliminate the invading bacteria.</p> Signup and view all the answers

What is the binding site of a protein receptor, and how does it interact with a ligand?

<p>The binding site is the region of a protein receptor that associates with a ligand, and they interact through formation of weak, noncovalent interactions, such as hydrogen bonds, electrostatic attractions, and van der Waals attractions, and favorable hydrophobic forces.</p> Signup and view all the answers

What is the significance of plasma membrane receptors in determining cellular response to ligands?

<p>Plasma membrane receptors determine which ligands a cell will bind with and how the cell will respond to binding, regulating the cell's response to hormones, neurotransmitters, and other molecules.</p> Signup and view all the answers

How do receptors for endorphins and opiates modulate pain perception?

<p>Receptors for endorphins and opiates change the cell's permeability to ions, increase the concentration of molecules that regulate intracellular protein synthesis, and initiate molecular events that modulate pain perception.</p> Signup and view all the answers

What is the function of antigen receptors on white blood cells?

<p>Antigen receptors on white blood cells recognize and bind with antigenic microorganisms, activating the immune and inflammatory responses.</p> Signup and view all the answers

What is the outcome of ligand binding to a receptor, and how is it regulated?

<p>Ligand binding to a receptor activates or inhibits the receptor's associated signaling or biochemical pathway, and is regulated by the specificity and number of receptors present on the plasma membrane.</p> Signup and view all the answers

What is the role of hormone binding in regulating protein synthesis within the cell?

<p>Hormone binding regulates protein synthesis within the cell through special messenger molecules that regulate protein synthesis.</p> Signup and view all the answers

What is the significance of membrane receptors in the regulation of cellular function?

<p>Membrane receptors regulate cellular function by determining which ligands a cell will bind with and how the cell will respond to binding, influencing cellular responses to hormones, neurotransmitters, and other molecules.</p> Signup and view all the answers

What is the outcome of receptor binding to a ligand, and how does it influence cellular response?

<p>Receptor binding to a ligand activates or inhibits the receptor's associated signaling or biochemical pathway, influencing cellular response to hormones, neurotransmitters, and other molecules.</p> Signup and view all the answers

What is the significance of receptor classification based on location and function?

<p>Receptor classification based on location and function helps to understand their specific roles in regulating cellular function and response to external stimuli.</p> Signup and view all the answers

What is the main purpose of the extracellular matrix in cells?

<p>The main purpose of the extracellular matrix is to provide mechanical support, control cell proliferation, form a scaffold for tissue regeneration, and establish tissue microenvironments.</p> Signup and view all the answers

What is the difference between the extracellular matrix and the basement membrane?

<p>The extracellular matrix is an intricate meshwork of interstitial fibrous proteins, while the basement membrane is a thin, tough, and flexible sheet of matrix molecules that lies beneath epithelial cells.</p> Signup and view all the answers

How do cells adhere to each other and form tissues?

<p>Cells adhere to each other through cell-to-cell adhesions, facilitated by cell adhesion molecules in the cell's plasma membrane, and are held together by the extracellular matrix and specialized cell junctions.</p> Signup and view all the answers

What is the primary function of the plasma membrane in cells?

<p>The primary function of the plasma membrane is to serve as the outer boundary of the cell and allow groups of cells to be held together robustly.</p> Signup and view all the answers

What is the primary function of collagen in the extracellular matrix?

<p>To provide tensile strength or resistance to longitudinal stress.</p> Signup and view all the answers

How do cells lift heavy objects like a textbook?

<p>Cells can lift heavy objects like a textbook by forming muscles, which are robust tissues that are held together by cell-to-cell adhesions and the extracellular matrix.</p> Signup and view all the answers

What is the role of the extracellular matrix in regulating cell growth and movement?

<p>The extracellular matrix provides a pathway for diffusion of nutrients, wastes, and other water-soluble traffic between the blood and tissue cells, and helps regulate cell growth, movement, and differentiation.</p> Signup and view all the answers

What is the role of elastin in the extracellular matrix?

<p>To provide elasticity and allow for stretching and recoiling in tissues such as the lungs.</p> Signup and view all the answers

What is the significance of cell-to-cell adhesions in tissue formation?

<p>Cell-to-cell adhesions are crucial for forming tissues and organs, as they allow cells to be held together robustly and enable the formation of robust tissues.</p> Signup and view all the answers

What is the function of fibronectin in the extracellular matrix?

<p>To promote cell adhesion and cell anchorage.</p> Signup and view all the answers

What happens to the fibrils in cartilage when collagen breaks down, such as in osteoarthritis?

<p>The fibrils that give cartilage its tensile strength are destroyed.</p> Signup and view all the answers

How do cells interact with their environment?

<p>Cells interact with their environment through the extracellular matrix, which provides a pathway for diffusion of nutrients, wastes, and other water-soluble traffic between the blood and tissue cells.</p> Signup and view all the answers

What is the role of the extracellular matrix in connective tissue?

<p>To connect cells together to form tissues and organs.</p> Signup and view all the answers

What is the role of specialized cell junctions in tissue formation?

<p>Specialized cell junctions help hold cells together and facilitate the formation of robust tissues and organs.</p> Signup and view all the answers

What is the function of fibroblasts in the extracellular matrix?

<p>To secrete the extracellular matrix.</p> Signup and view all the answers

What is the main function of the basement membrane?

<p>The main function of the basement membrane is to provide a thin, tough, and flexible sheet of matrix molecules that lies beneath epithelial cells and surrounds individual muscle cells, fat cells, and Schwann cells.</p> Signup and view all the answers

What is the significance of the extracellular matrix beyond providing a passive scaffold for cellular attachment?

<p>It helps regulate the functions of the cells within which it interacts, including cell growth, movement, and differentiation.</p> Signup and view all the answers

What is the composition of most human connective tissues?

<p>The majority of the tissue is composed of extracellular matrix, with cells scattered within it.</p> Signup and view all the answers

What is the role of hyaluronic acid in the extracellular matrix?

<p>It is a component of the extracellular matrix, along with proteoglycans, collagen, and elastin.</p> Signup and view all the answers

What is the significance of the diversity of human connective tissues?

<p>They can be hard and dense, like bone; flexible, like tendons or the dermis of the skin; resilient and shock absorbing, like cartilage; or soft and transparent, like the jelly-like substance that fills the eye.</p> Signup and view all the answers

What is the primary function of integrins in cell adhesion molecules?

<p>to regulate cell-ECM interactions with collagen, fibronectin, vitronectin, and fibrinogen</p> Signup and view all the answers

What is the unique characteristic of cadherins in cell adhesion molecules?

<p>They are Ca++-dependent glycoproteins</p> Signup and view all the answers

What is the function of selectins in cell adhesion molecules?

<p>to bind certain carbohydrates, for example, mucins</p> Signup and view all the answers

What is the purpose of tight junctions in cell junctions?

<p>to form a tight seal and hold cells together</p> Signup and view all the answers

What is the function of gap junctions in cell junctions?

<p>to provide a special type of chemical communication, for example, inorganic ions and small water-soluble molecules</p> Signup and view all the answers

What is the classification of hemidesmosomes in cell junctions?

<p>asymmetrical junctions</p> Signup and view all the answers

What is the function of cell adhesion molecules (CAMs) in cell-cell interactions?

<p>to bind the cell to an adjacent cell and to components of the extracellular matrix (ECM)</p> Signup and view all the answers

What is the classification of desmosomes in cell junctions?

<p>symmetrical junctions</p> Signup and view all the answers

What is the purpose of adherens junctions in cell junctions?

<p>to provide strong mechanical attachments</p> Signup and view all the answers

What is the function of immunoglobulin (Ig) superfamily CAMs in cell adhesion molecules?

<p>to bind integrins or other IgSF CAMs</p> Signup and view all the answers

What is the function of desmosomes in epithelial cells?

<p>They unite cells and maintain structural stability by forming continuous bands or belts of epithelial sheets or developing button-like points of contact.</p> Signup and view all the answers

What is the role of tight junctions in epithelial cells?

<p>They act as barriers to diffusion, preventing the movement of substances through transport proteins in the plasma membrane and leakage of small molecules between the plasma membranes of adjacent cells.</p> Signup and view all the answers

What is the function of gap junctions in epithelial cells?

<p>They allow small ions and molecules to pass directly from the inside of one cell to the inside of another through communicating tunnels or connexons.</p> Signup and view all the answers

What is the junctional complex in epithelial cells?

<p>It is a highly permeable part of the plasma membrane formed by the zones between epithelial cells, including the zonula occludens, zonula adherens, and macula adherens.</p> Signup and view all the answers

What is the result of increased cytoplasmic calcium concentration on the junctional complex?

<p>It causes decreased permeability at the junctional complex.</p> Signup and view all the answers

What happens when damaged cells release calcium into the junctional complex?

<p>It travels through the junctional complex and increases calcium levels in neighboring cells, causing damaging effects.</p> Signup and view all the answers

What is the outcome when the junctional complex of neighboring cells becomes impermeable?

<p>It forms a relatively impermeable wall around the injured area, sealing off the damaged cells.</p> Signup and view all the answers

What is the function of the zonula occludens in epithelial cells?

<p>It is a part of the junctional complex that forms a barrier to diffusion and prevents the leakage of small molecules between adjacent cells.</p> Signup and view all the answers

What is the role of the zonula adherens in epithelial cells?

<p>It is a part of the junctional complex that helps to unite cells and maintain structural stability.</p> Signup and view all the answers

What is the function of the macula adherens in epithelial cells?

<p>It is a part of the junctional complex that helps to unite cells and maintain structural stability.</p> Signup and view all the answers

What is the primary function of gap junctions in cellular communication?

<p>To directly coordinate the activities of adjacent cells.</p> Signup and view all the answers

What type of signaling requires cells to be in close membrane-membrane contact?

<p>Contact-dependent signaling</p> Signup and view all the answers

What type of signaling involves the secretion of local chemical mediators that are quickly absorbed, destroyed, or immobilized?

<p>Paracrine signaling</p> Signup and view all the answers

What is the term for the signaling mechanism where cells respond to signals they produce themselves?

<p>Autocrine signaling</p> Signup and view all the answers

What is the result of alterations in cellular communication, leading to disease onset and progression?

<p>Disease onset and progression</p> Signup and view all the answers

What is the primary function of receptors in cellular communication?

<p>To affect the cell itself and other cells in direct physical contact</p> Signup and view all the answers

What is the term for the protein channels that directly coordinate the activities of adjacent cells?

<p>Gap junctions</p> Signup and view all the answers

What is the result of a cell's inability to perform gap junctional intercellular communication?

<p>Compromised normal growth control and cell differentiation, favoring cancerous tumor development</p> Signup and view all the answers

What is the primary mode of cellular communication that involves secreted chemical signals?

<p>Hormonal signaling</p> Signup and view all the answers

What is the term for the primary modes of intercellular signaling?

<p>Contact-dependent, paracrine, hormonal, neurohormonal, and neurotransmitter signaling</p> Signup and view all the answers

What is the primary mechanism by which hormones communicate with their target cells?

<p>Hormones are released by endocrine cells and travel through the bloodstream to produce a response in other sets of cells.</p> Signup and view all the answers

What is the key difference between neurohormonal signaling and ordinary neuronal signaling?

<p>Neurohormonal signaling involves the release of blood-borne chemical messengers, whereas ordinary neuronal signaling involves the release of short-range neurotransmitters at synapses.</p> Signup and view all the answers

What is the role of signal transduction pathways in cellular communication?

<p>Signal transduction pathways transmit signals from the cell surface to the cell interior, amplifying and distributing the signal to multiple targets.</p> Signup and view all the answers

What is the outcome when cells are deprived of appropriate signals?

<p>Cells undergo programmed cell death, or apoptosis.</p> Signup and view all the answers

How do signal transduction pathways amplify the original signal?

<p>Signal transduction pathways amplify the signal through a multiplying effect, where one ligand molecule can activate multiple downstream targets.</p> Signup and view all the answers

What is the primary function of plasma membrane receptors?

<p>Plasma membrane receptors recognize and respond specifically to extracellular signal molecules.</p> Signup and view all the answers

What is the outcome when cells receive a signal through binding of an extracellular signaling messenger to a membrane receptor?

<p>The signal can either open or close specific channels in the membrane, or transfer the signal to an intracellular messenger.</p> Signup and view all the answers

What is the significance of signal transduction pathways in cellular responses?

<p>Signal transduction pathways allow cells to respond to their environment, influencing processes such as cell growth, survival, and differentiation.</p> Signup and view all the answers

What is the role of protein kinases in signal transduction pathways?

<p>Protein kinases activate a path of intracellular signaling molecules, leading to cellular responses such as growth, survival, or differentiation.</p> Signup and view all the answers

What is the primary function of hormones in intercellular signaling?

<p>Hormones are chemical messengers that allow cells to communicate with each other.</p> Signup and view all the answers

Study Notes

Cellular Biology

  • Understanding cellular biology is crucial for understanding disease
  • Cellular biology is integral to understanding how cells behave as a multicellular organism
  • Cellular communication (cellular crosstalk) is at the heart of cellular biology
  • Cellular crosstalk involves the origination, transmission, reception, interpretation, and utilization of messages between, among, and within cells
  • This streamlined conversation maintains cellular function and specialization
  • Intercellular signals enable each cell to determine its position and specialized role

Cellular Function and Disease

  • Cells must demonstrate a "chemical fondness" for other cells and their surrounding environment to maintain the integrity of the entire organism
  • When cells no longer tolerate this fondness, the conversation breaks down
  • Breakdown of cellular communication can lead to cells either adapting (sometimes altering function) or becoming vulnerable to:
    • Isolation
    • Injury
    • Disease
    • Death

Cell Classification

  • Living cells are divided into two major classes: eukaryotes and prokaryotes.
  • Eukaryotes include cells of higher animals and plants, fungi, protozoa, and most algae.
  • Prokaryotes include cyanobacteria, bacteria, and rickettsiae.

Eukaryotic Cell Characteristics

  • Eukaryotes are larger and have more extensive intracellular anatomy and organization than prokaryotes.
  • Eukaryotic cells have a characteristic set of membrane-bound intracellular compartments called organelles.
  • Organelles include a well-defined nucleus.
  • Eukaryotic cells have multiple chromosomes.

Prokaryotic Cell Characteristics

  • Prokaryotes contain no organelles.
  • Prokaryotic cells lack a distinct nucleus.
  • The nuclear material is not encased by a nuclear membrane.
  • Prokaryotes have a single circular chromosome.
  • Prokaryotes lack histones, which are proteins that bind with DNA and are involved in the supercoiling of DNA.

Cellular Differences

  • Chemical composition and biochemical activity differ between prokaryotic and eukaryotic cells.
  • Protein production (synthesis) differs between the two classes of cells due to major structural differences in RNA-protein complexes.
  • Mechanisms of transport across the outer cellular membrane differ between prokaryotic and eukaryotic cells.
  • Enzyme content differs between prokaryotic and eukaryotic cells.

Cellular Specialization

  • Cells become specialized through differentiation, allowing them to perform specific functions.
  • Specialized cells often lack certain properties, but excel in others.

Eight Chief Cellular Functions

Movement

  • Muscle cells generate forces to produce motion.
  • Examples: muscle cells attached to bones produce limb movements, and smooth muscle cells surrounding blood vessels change diameter.

Conductivity

  • Conduction is a response to a stimulus, manifesting as a wave of excitation.
  • Nerve cells primarily function for conductivity.

Metabolic Absorption

  • Cells take in and use nutrients and substances from their surroundings.
  • Examples: intestinal cells reabsorb fluids and synthesize protein enzymes, and kidney cells reabsorb fluids and synthesize proteins.

Secretion

  • Certain cells synthesize new substances and secrete them as needed.
  • Examples: mucous gland cells, adrenal gland cells, and ovary cells secrete hormonal steroids.

Excretion

  • Cells rid themselves of waste products through membrane-bound sacs (lysosomes) containing enzymes.
  • Lysosomes break down large molecules into waste products released from the cell.

Respiration

  • Cells absorb oxygen to transform nutrients into energy in the form of adenosine triphosphate (ATP).
  • Cellular respiration occurs in mitochondria.

Reproduction

  • Tissue growth occurs through cell enlargement and reproduction.
  • Cellular division is necessary for tissue maintenance and replacement.

Communication

  • Communication is vital for cells to survive as a society.
  • Examples: pancreatic cells secrete insulin to signal muscle cells to absorb sugar from the blood.

Eukaryotic Cell Structure

  • A eukaryotic cell consists of three components: plasma membrane, cytoplasm, and organelles.
  • The plasma membrane, also known as the plasmalemma, is the outer membrane of the cell.

Nucleus

  • The nucleus is the largest membrane-bound organelle, located in the center of the cell, surrounded by cytoplasm.
  • The nuclear envelope is composed of two pliable membranes with nuclear pores, allowing molecules to move between the nucleus and cytosol.
  • The nucleus contains the nucleolus, cellular DNA, and histone proteins that regulate DNA activity.
  • Histones binding to DNA causes DNA to fold into chromosomes, which is essential for cell division in eukaryotes.

Functions of the Nucleus

  • The primary functions of the nucleus are cell division and control of genetic information.
  • The nucleus is also responsible for DNA replication and repair, and transcription of genetic information into RNA.
  • RNA is processed into messenger, transport, and ribosomal RNA and introduced into the cytoplasm, where it directs cellular activities.

Cytoplasm

  • Cytoplasm is an aqueous solution (cytosol) that fills the space between the nuclear envelope and the plasma membrane.
  • Cytosol represents about half the volume of a eukaryotic cell and contains thousands of enzymes involved in intermediate metabolism.
  • Cytosol is the main site for protein synthesis and degradation, and is crowded with ribosomes that make proteins.

Organelles

  • Organelles suspended in the cytoplasm are enclosed in biologic membranes, enabling them to carry out functions that require different biochemical environments.
  • Organelle functions include protein synthesis and transport, hormone synthesis, waste elimination, metabolic processes, and maintenance of cellular structure and motility.

Ribosomes

  • Ribosomes are RNA-protein complexes synthesized in the nucleolus and secreted into the cytoplasm through nuclear pore complexes (NPCs)
  • They can float free in the cytoplasm or attach to the outer membranes of the endoplasmic reticulum (ER)
  • Their chief function is to provide sites for cellular protein synthesis
  • Newly formed ribosomes synthesize a “recognition sequence,” or signal, like an address on a letter
  • Signal recognition particles (SRPs) in the cytosol bind to the ribosome after recognizing the SRP
  • Ribophorins, receiver proteins found on the rough sections of the ER, act as the “address” site or binding site
  • The developing protein threads its way through the ER membrane into the lumen
  • The SRP is removed and the new protein chain is folded into its final conformation

Endoplasmic Reticulum (ER)

  • The ER is a membrane factory that specializes in the synthesis and transport of protein and lipid components of most of the cell's organelles
  • It consists of a network of tubular or saclike channels (cisternae) that extend throughout the cytoplasm and are continuous with the outer nuclear membrane
  • The ER may be rough (granular) or smooth (agranular)
  • Rough ER (rER) is rough because ribosomes and ribonucleoprotein particles are attached to it
  • Some of the proteins synthesized by these ribosomes remain in the ER, and others are used to construct membranes of other organelles
  • The ER is responsible for much of a cell's protein synthesis and folding, and senses cellular stress

Protein Folding

  • Protein folding in the ER is critical for us
  • Proteins must fold into complex three-dimensional structures to perform vital functions
  • Most secreted proteins fold and are modified in an error-free manner, but ER or cell stress, mutations, or random errors during protein synthesis can decrease the folding amount or rate
  • Pathophysiologic processes, environmental toxins, and mutant protein expression can perturb the sensitive ER environment
  • The ER has protective ways to help folding, such as protein chaperones
  • Misfolded proteins not repaired in the ER can lead to ER stress and initiate apoptosis or cell death

Unfolded-Protein Response (UPR)

  • The ER mediates intracellular signaling pathways in response to the accumulation of unfolded or misfolded proteins
  • The UPR is an adaptive response to ER stress
  • Investigators are studying UPR-associated inflammation and how the UPR is coupled to inflammation in health and disease
  • Specific diseases associated with ER stress include Alzheimer disease, Parkinson disease, prion disease, amyotrophic lateral sclerosis, diabetes mellitus, and sepsis

Smooth Endoplasmic Reticulum (sER)

  • sER does not contain ribosomes or ribonucleoprotein particles
  • Membranous surfaces of the sER contain enzymes involved in the synthesis of steroid hormones
  • sER is responsible for a variety of reactions required to remove toxic substances from the cell

Golgi Complex

  • The Golgi complex is a network of flattened, smooth membranes and vesicles frequently located near the nucleus of the cell
  • Proteins from the ER are processed and packaged into small membrane-bound sacs or vesicles called secretory vesicles
  • Secretory vesicles collect at the end of the membranous folds of the Golgi bodies—called cisternae
  • The vesicles then break off from the Golgi complex and migrate to a variety of intracellular and extracellular destinations, including the plasma membrane
  • The vesicles fuse with the plasma membrane, and their contents are released from the cell

Lysosomes: Structure and Function

  • Lysosomes are membrane-enclosed organelles that contain over 60 digestive enzymes (hydrolases) that break down macromolecules, defunct organelles, and external particles engulfed by endocytosis.
  • Hydrolases function optimally at an acidic pH, catalyzing bonds in proteins, lipids, nucleic acids, and carbohydrates.

Intracellular Digestive System

  • Lysosomes act as the intracellular digestive system, capable of breaking down cellular constituents to their basic components (amino acids, nucleotides, and carbohydrates).
  • Transport proteins in the lysosome membrane carry these components to the cytosol, where the cell can reuse or excrete them.

Signaling Function

  • Lysosomes have a newly discovered signaling function, cooperating with their degradative role to mediate basic cellular functions (nutrient-dependent signal transduction for cellular adaptation).
  • Complex transcriptional programs control lysosome synthesis, composition, and quantity, regulating their activity to match cellular needs.

Cellular Health and Disease

  • Lysosomes maintain cellular health by efficiently removing toxic cellular components, useless organelles, and terminating signal transduction.
  • Alterations in lysosomal function are central to the pathophysiology of various conditions, including storage diseases, neurodegenerative diseases, and cancer.
  • Aging leads to a progressive loss of lysosomal efficiency and decline of regenerative capacity in organs and tissues.

Lysosomes as Signaling Hubs

  • Lysosomes are key signaling hubs, integrating functions such as nutrient abundance, energy levels, and cell stressors to regulate cellular metabolism.
  • The signaling functions have far-reaching implications for metabolic regulation in health and disease.

Lysosomal Function

  • Lysosomal membrane acts as a protective shield between the powerful digestive enzymes within the lysosome and the cytoplasm, preventing their leakage into the cytoplasmic matrix.
  • Disruption of the membrane leads to a release of the lysosomal enzymes, causing cellular self-digestion.

Lysosomal Abnormalities

  • Lysosomal abnormalities are involved in various conditions that involve cellular injury and death.
  • Lysosomal storage diseases (LSDs) can result from a genetic defect or lack of one or more lysosomal enzymes.

Examples of Lysosomal Storage Diseases

  • Pompe disease: lack of lysosomal α-1,4-glucosidase leads to an accumulation of glycogen in lysosomes.
  • Tay-Sachs disease: deficiency or absence of lysosomal hexosaminidase A leads to an accumulation of GM2 ganglioside in lysosomes.
  • Gout: undigested uric acid accumulates within lysosomes, damaging the lysosomal membrane.

Pathways of Degradation in Lysosomes

  • Four pathways: endocytosis, phagocytosis, macropinocytosis, and autophagy.
  • Endocytosis: uptake of macromolecules from extracellular fluid.
  • Phagocytosis: engulfment of large particles or microorganisms in phagocytic cells.
  • Macropinocytosis: nonspecific uptake of fluids, membrane, and particles attached to the plasma membrane.
  • Autophagy: self-eating, begins in the cytosol, used to digest cytosol and ineffectual organelles.

Lysosomal Activation

  • Lysosomes remain fully active by maintaining a low internal pH.
  • Hydrolytic enzymes are only maximally active at acidic pH values.
  • Primary lysosomes: not active, do not maintain acidic internal pH.
  • Secondary lysosomes (or heterophagosomes): formed when primary lysosome fuses with a vacuole or other organelle, pH decreases, and hydrolytic enzymes become activated.

Autophagy and Autolysosomes

  • Autophagy: promotes homeostasis, involves continuous biosynthesis and cell turnover, plays a crucial role in health.
  • Autolysosomes (or autophagosomes): involved in autophagy, digest cellular debris and obsolete parts.
  • Defects in autophagy may challenge disposal mechanisms, contributing to disease.

Autophagy Products

  • Products of autophagy (and phagocytosis) pass out of the lysosome and are reused by the cell.
  • Indigestible material is stored in residual bodies, whose contents are actively expelled from the cell.
  • High concentrations of lipids may accumulate within residual bodies, eventually forming lipofuscin, a pigmented substance.

Peroxisomes

  • Peroxisomes are membrane-bound organelles containing oxidative enzymes like catalase and urate oxidase.
  • These enzymes detoxify compounds and fatty acids by removing hydrogen atoms from specific substrates, producing hydrogen peroxide (H2O2).
  • Peroxisomes are larger and oval or irregular in shape, similar to lysosomes in microscopic appearance.
  • They are major sites of oxygen utilization, similar to mitochondria.
  • Catalase, an antioxidant enzyme, uses H2O2 to oxidize various substrates, breaking down H2O2 into H2O and O2.
  • Peroxisomes play a crucial role in synthesizing specialized phospholipids necessary for nerve cell myelination.
  • Impairment of peroxisomes can lead to disease.

Mitochondria

  • Mitochondria are organelles found in large numbers in most cells, responsible for cellular respiration and energy production.
  • They appear as spheres, rods, or filamentous bodies bound by a double membrane.
  • The outer membrane is smooth, while the inner membrane is convoluted in the mitochondrial matrix to form cristae.
  • The inner membrane contains enzymes of the respiratory chain, essential for oxidative phosphorylation and generating most of the cell's ATP.
  • Metabolic pathways involved in carbohydrate, lipid, and amino acid metabolism, as well as urea and heme synthesis, are located in the mitochondrial matrix.
  • The outer membrane is permeable to many substances, while the inner membrane is highly selective and contains transmembranous transport systems.
  • Mitochondria contain their own DNA, which codes for enzymes needed for oxidative phosphorylation.

Cytosol Structure and Function

  • Cytosol is the gelatinous, semiliquid portion of the cytoplasm, making up about 55% of the total cell volume.

Intermediary Metabolism

  • Intermediary metabolism involves the synthesis, degradation, and transformation of small organic molecules (e.g., simple sugars, fatty acids, and amino acids).
  • These reactions occur in the cytoplasm, with most of the metabolism taking place in the cytosol.
  • Intermediary metabolism enables energy to be used for managing cellular activities and for providing substrates to maintain cell integrity.

Protein Synthesis

  • Ribosomal protein synthesis takes place in free ribosomes in the cytosol.
  • Cytosolic ribosomes that synthesize identical proteins are collected in "factories" known as polyribosomes.

Storage and Transport

  • Excess stored nutrients not immediately used for ATP production are converted in the cytosol into storage forms (e.g., excess glucose is stored as glycogen).
  • These temporary storage forms are known as inclusions.
  • Secretory vesicles that have been processed and packaged by the endoplasmic reticulum and Golgi complex remain in the cytosol.
  • These vesicles transport and empty their contents outside the cell through signaling.

Cytoskeleton

  • The cytoskeleton is a network of protein filaments that provides the "bones and muscles" of the cell, maintaining cell shape and internal organization, and permitting movement of substances within the cell and external projections.
  • The cytoskeleton is composed of three main types of filaments: actin filaments, microtubules, and intermediate filaments.

Mechanotransduction

  • Mechanotransduction is the process by which cells translate mechanical stimuli into biochemical signals, allowing cells to adapt to their surroundings.
  • Cell stresses involved in mechanotransduction are associated with several alterations and diseases, including loss of hearing, cardiovascular disease, muscular dystrophy, and cancer.

Microtubules

  • Microtubules are small, hollow, cylindrical, unbranched tubules made of protein that add strength to the cell's structure.
  • Microtubules support and move organelles within the cell, facilitate transport of impulses along nerve cells, and have roles in inflammatory and immune responses and hormone secretion.
  • Microtubules are also involved in external movement, or motility, of some cells, such as the movement of sperm cells and cilia on cells lining the respiratory tract.

Centrioles and Cellular Division

  • During cellular division (mitosis), microtubules involved in cellular division are arranged in a centriole, which consists of nine bundles containing three microtubules each.
  • The pairs of centrioles split and migrate to opposite poles of the cell during division.

Actin Filaments (Microfilaments)

  • Actin filaments are smaller fibrils that occur in bundles, concentrated in the cell cortex just beneath the plasma membrane.
  • Actin filaments link the interior of the cell to adjacent cells through cell junctions and are necessary for regulating cell growth and driving cellular locomotion.

Intermediate Filaments

  • Intermediate filaments are braided, ropelike fibers made of several filament proteins that form a mesh called the nuclear lamina beneath the inner nuclear membrane, creating a protective chamber for the cell's DNA.
  • Intermediate filaments also crisscross the cytoplasm, promoting mechanical strength, and in epithelial tissue, they bridge the cytoplasm from one cell junction to another, supporting and strengthening the sheet of epithelium.

Cell Membrane Structure and Function

  • Membranes define cell boundaries and control the composition of the compartment they enclose by allowing or excluding various molecules.
  • Membranes exert a powerful influence on metabolic pathways by controlling the movement of substances from one compartment to another.

Cell Polarity and Transport

  • Directional transport is facilitated by polarized domains, distinct apical and basolateral domains.
  • Cell polarity maintains normal cell and tissue structure, importantly for transport of nutrients in and out of the cell.

Plasma Membrane Functions

  • The plasma membrane has an important role in cell-to-cell recognition, with protein receptors for hormones and chemical signals acting as markers.
  • The plasma membrane assists with cellular mobility and maintenance of cellular shape.

Cell Membrane Components

  • The main components of cell membranes are lipids and proteins.
  • The basic structure of cell membranes is the lipid bilayer, composed of two continuous opposing leaflets and proteins that span the bilayer.

Lipid Bilayer Structure and Function

  • The lipid bilayer provides the basic fluid structure of the membrane and is mostly an impermeable barrier to water-soluble molecules.
  • Individual lipid molecules can diffuse readily throughout their own monolayer.

Lipid Rafts and Domains

  • Lipids and proteins are not distributed uniformly but are seen to concentrate in a temporary, dynamic way assisted by protein-protein interactions.
  • Lipid rafts in living cells may be important for cell communication, where protein assemblies convert extracellular signals into intracellular signals.

Phospholipids and Lipid Classes

  • Phospholipids are key for repairing the membrane and tend to spontaneously rearrange themselves to avoid a tear.
  • Inositol phospholipids are a subclass of phospholipids important for cell signaling because they respond to extracellular signals in the cytosolic lipid leaflet of the bilayer.

Amphipathic Molecules and Bilayer Structure

  • An amphipathic molecule has a hydrophobic (uncharged) part and a hydrophilic (charged) part.
  • The membrane spontaneously organizes itself into a bilayer because of these two incompatible solubilities.

Bilayer Function and Permeability

  • The bilayer is impermeable to most water-soluble molecules because they are insoluble in the oily core region.
  • The bilayer serves as a barrier to the diffusion of water and hydrophilic substances while allowing lipid-soluble molecules to diffuse through it readily.

Proteins in the Plasma Membrane

  • Proteins perform most of the plasma membrane's specific tasks, with varying amounts and types of proteins in a membrane.
  • Proteins are made from a chain of amino acids, known as polypeptides, with 20 types of amino acids in proteins, and each type of protein has a unique sequence of amino acids.

Association of Proteins with the Lipid Bilayer

  • Membrane proteins associate with the lipid bilayer in different ways, including:
  • Transmembrane proteins extending across the bilayer and exposed to an aqueous environment on both sides.
  • Proteins located almost entirely in the cytosol, associated with the cytosolic half of the lipid bilayer by an α helix exposed on the surface of the protein.
  • Proteins existing outside the bilayer, on one side or the other, and attached to the membrane by one or more covalently attached lipid groups.
  • Proteins bound indirectly to one or the other bilayer membrane face and held in place by their interactions with other proteins.

Properties of Membrane Proteins

  • Membrane proteins are amphiphilic, with both hydrophobic and hydrophilic regions.
  • Proteins exist in densely folded molecular configurations, with an excess of hydrophilic units at the surface of the molecule and an excess of hydrophobic units inside.

Synthesis and Trafficking of Membrane Proteins

  • Membrane proteins are synthesized mainly by ribosomes in the cytosol and then travel to their destinations.
  • The fate of a protein depends on its amino acid sequence, which contains sorting signals that direct its delivery to locations outside the cytosol or to organelle surfaces.
  • Proteins move from one compartment to another by gated transport, protein translocation, or vesicular transport.

Functions of Membrane Proteins

  • Proteins facilitate transport across membranes by serving as:
  • Receptors for substances moving in and out of the cell.
  • Pores or transport channels for ions, electrolytes, amino acids, and monosaccharides.
  • Enzymes that drive active pumps to promote concentration of certain ions within the cell.
  • Cell surface markers, such as glycoproteins, that identify a cell to its neighbor.
  • Cell adhesion molecules that allow cells to hook together and form attachments to the cytoskeleton for maintaining cellular shape.
  • Catalysts of chemical reactions, such as the conversion of lactose to glucose.

Proteostasis and Cellular Health

  • Proteostasis is a state of cell balance of the processes of protein synthesis, folding, and degradation, which is vital to cellular health.
  • The cellular protein pool is in constant change or flux, depending on how quickly proteins are made and how long they survive or are broken down.
  • The proteostasis network, comprising ribosomes, chaperones, and two protein breakdown systems (lysosomes and the ubiquitin-proteasome system), regulates protein homeostasis under various conditions.
  • Malfunction or failure of the proteostasis network is associated with human disease.

Proteases and Proteolytic Cascades

  • Proteases are enzymes that break down proteins and can be tethered to cell membranes.
  • Proteases participate in a tightly orchestrated sequence of events called a proteolytic cascade, which regulates essential physiological processes.
  • Four major proteolytic cascades with disease relevance are cell death or caspase-mediated apoptosis, blood coagulation cascade, degrading membrane enzymes or matrix metalloproteinase cascade, and the complement cascade.
  • Proteases within a proteolytic cascade can act as initiators, amplifiers, propagators, or executioners.

Cell Surface Carbohydrates

  • Cell surface carbohydrates are short chains of sugars or oligosaccharides attached to membrane proteins (glycoproteins) and lipids (glycolipids).
  • Long polysaccharide chains attached to membrane proteins are called proteoglycans.
  • The carbohydrate coating on the outside of the plasma membrane is called the glycocalyx or cell coat, which helps protect the cell from mechanical damage and provides a slimy surface for cell mobility.

Cellular Recognition and Adhesion

  • The glycocalyx facilitates cell-cell recognition and adhesion.
  • Intercellular recognition is possible through membrane oligosaccharides that bind to specific lectins, which recognize neutrophils at the site of bacterial infection.

Cellular Receptors

  • Cellular receptors are protein molecules that recognize and bind to specific smaller molecules called ligands.
  • Receptors can be found on the plasma membrane, in the cytoplasm, or in the nucleus.
  • Binding of ligands to receptors depends on the chemical configuration of the receptor and its ligand, and involves weak, noncovalent interactions.

Receptor Function

  • Binding of ligands to receptors activates or inhibits the receptor's associated signaling or biochemical pathway.
  • Receptors determine which ligands a cell will bind with and how the cell will respond to binding.
  • Examples of ligands include hormones and neurotransmitters.

Cell Membrane and Adhesions

  • Cells are enclosed by a flimsy membrane, but they can combine strongly to form tissues and organs.
  • Plasma membranes allow cells to be held together robustly through cell-to-cell adhesions.

Extracellular Matrix (ECM)

  • ECM is an intricate meshwork of interstitial fibrous proteins embedded in a watery, gel-like substance composed of complex carbohydrates.
  • ECM is secreted by fibroblasts and provides a pathway for diffusion of nutrients, wastes, and other water-soluble traffic between the blood and tissue cells.
  • ECM helps regulate cell growth, movement, and differentiation, and has four major functions: mechanical support, control of cell proliferation, formation of a scaffold for tissue regeneration, and establishment of tissue microenvironments.

Components of ECM

  • Fibrous structural proteins: collagen and elastin
  • Adhesive glycoproteins: fibronectin
  • Proteoglycans and hyaluronic acid

Functions of ECM Components

  • Collagen: provides tensile strength or resistance to longitudinal stress
  • Elastin: provides elasticity to tissues that must be capable of stretching and recoiling
  • Fibronectin: promotes cell adhesion and cell anchorage

Connective Tissue

  • Connective tissue is composed of ECM and the cells that produce it (e.g., fibroblasts)
  • Connective tissue connects cells together to form tissues and organs
  • Examples of human connective tissues: bone, tendons, dermis of the skin, cartilage, and the jelly-like substance that fills the eye.

Cell Adhesion Molecules (CAMs)

  • CAMs are cell surface proteins that bind cells to adjacent cells and components of the extracellular matrix (ECM)
  • There are four protein families of CAMs: integrins, cadherins, selectins, and immunoglobulin (Ig) superfamily

Integrins

  • A major class of receptors within the ECM
  • Regulate cell-ECM interactions with collagen, fibronectin, vitronectin, and fibrinogen

Cadherins

  • Ca++-dependent glycoproteins with unique tissue distribution patterns
  • Example: epithelial (E cadherin)

Selectins

  • Bind certain carbohydrates, such as mucins

Immunoglobulin Superfamily (IgSF) CAMs

  • Bind integrins or other IgSF CAMs

Cell Junctions

  • Specialized regions of plasma membranes that link cells together
  • Classified by function:
    • Tight junctions: hold cells together, forming a tight seal
    • Adherens junctions, desmosomes, and hemidesmosomes: provide strong mechanical attachments
    • Gap junctions: allow chemical communication between cells
    • Maintain apico-basal polarity of individual epithelial cells (tight junctions)

Classification of Cell Junctions

  • Symmetrical junctions: tight junctions (zonula occludens), belt desmosome (zonula adherens), desmosomes (macula adherens), and gap junctions
  • Asymmetrical junction: hemidesmosome

Functions of Cell Junctions

  • Make the epithelium leak-proof by mediating mechanical attachment of one cell to another
  • Allow communicating tunnels between cells
  • Maintain cell polarity

Cell Communication

  • Cells communicate to maintain homeostasis, regulate growth and division, and coordinate functions, using hundreds of signal molecules.
  • Three main ways of cell communication:
  • Displaying plasma membrane-bound signaling molecules (receptors) for direct physical contact.
  • Affecting receptor proteins inside target cells, requiring signal molecules to enter the cell.
  • Forming protein channels (gap junctions) for direct coordination between adjacent cells.

Intercellular Signaling Modes

  • Primary modes of intercellular signaling:
  • Contact-dependent signaling: requires close membrane-membrane contact.
  • Paracrine signaling: local chemical mediators are quickly absorbed, destroyed, or immobilized.
  • Hormonal signaling: specialized endocrine cells secrete hormones that travel through tissue and bloodstream.
  • Neurohormonal signaling: hormones are released by neurosecretory neurons.
  • Neurotransmitter signaling: neurons release short-range neurotransmitters into a small, discrete space (synapse).

Signal Transduction

  • Signal transduction involves incoming signals from extracellular chemical messengers (ligands) conveyed to the cell's interior for execution.
  • Cells respond to external stimuli by activating signal transduction pathways or signaling cascades.
  • Signals are passed between cells through receptor proteins that recognize and respond specifically to signal molecules.

Functions of Signal Transduction Pathways

  • Physically transfer the signal from the reception site to another part of the cell.
  • Amplify the signal received through a multiplying effect.
  • Distribute the signal to influence several processes in parallel, creating branches in the flow.
  • Modulate the signal by interfering factors inside or outside the cell.

Responses to Signaling

  • Two general responses to binding of extracellular signaling messengers (ligands) to membrane receptors:
  • Opening or closing specific channels in the membrane to regulate ion movement.
  • Transferring the signal to an intracellular messenger (second messenger), triggering a cascade of biochemical events within the cell.

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Explore the fundamental principles of cellular biology, focusing on cellular communication and its role in understanding disease. Learn how cells interact and respond to messages in a multicellular organism.

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