Podcast
Questions and Answers
What is the fundamental concept that underlies the understanding of disease?
What is the fundamental concept that underlies the understanding of disease?
The integrity of cells.
What is the term used to describe the process of how cells communicate with each other?
What is the term used to describe the process of how cells communicate with each other?
Cellular crosstalk
What is the purpose of intercellular signals in maintaining cellular function?
What is the purpose of intercellular signals in maintaining cellular function?
To allow cells to determine their position and specialized role.
What is the result when cells no longer demonstrate a 'chemical fondness' for other cells and their environment?
What is the result when cells no longer demonstrate a 'chemical fondness' for other cells and their environment?
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How do cells maintain the integrity of the entire organism?
How do cells maintain the integrity of the entire organism?
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What is the outcome when cells adapt to changes in their environment?
What is the outcome when cells adapt to changes in their environment?
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What are the two major classes of living cells?
What are the two major classes of living cells?
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What characteristic feature do eukaryotic cells have that prokaryotes lack?
What characteristic feature do eukaryotic cells have that prokaryotes lack?
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What type of organism are fungi, protozoa, and most algae?
What type of organism are fungi, protozoa, and most algae?
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What is the term for the membrane-bound compartments found in eukaryotic cells?
What is the term for the membrane-bound compartments found in eukaryotic cells?
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What type of chromosome do prokaryotic cells have?
What type of chromosome do prokaryotic cells have?
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What is the function of histones in eukaryotic cells?
What is the function of histones in eukaryotic cells?
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How do protein synthesis mechanisms differ between prokaryotic and eukaryotic cells?
How do protein synthesis mechanisms differ between prokaryotic and eukaryotic cells?
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What is a key difference in the mechanism of transport across the outer cellular membrane between prokaryotes and eukaryotes?
What is a key difference in the mechanism of transport across the outer cellular membrane between prokaryotes and eukaryotes?
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What is a distinction between prokaryotic and eukaryotic cells in terms of enzyme content?
What is a distinction between prokaryotic and eukaryotic cells in terms of enzyme content?
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Why are eukaryotic cells generally larger than prokaryotic cells?
Why are eukaryotic cells generally larger than prokaryotic cells?
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What is the primary function of muscle cells?
What is the primary function of muscle cells?
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What is the chief function of nerve cells?
What is the chief function of nerve cells?
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What is the specialized function of intestinal epithelial cells?
What is the specialized function of intestinal epithelial cells?
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What is the main function of cells in the kidney tubules?
What is the main function of cells in the kidney tubules?
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What is the function of mucous gland cells?
What is the function of mucous gland cells?
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How do cells eliminate waste products?
How do cells eliminate waste products?
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What is the primary function of cells in the adrenal gland, testis, and ovary?
What is the primary function of cells in the adrenal gland, testis, and ovary?
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What is the main difference between specialized cells in terms of function?
What is the main difference between specialized cells in terms of function?
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What is the outcome when cells become specialized?
What is the outcome when cells become specialized?
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What is the common function shared by all cells?
What is the common function shared by all cells?
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What is the primary site of RNA processing?
What is the primary site of RNA processing?
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What is the main site for protein synthesis and degradation?
What is the main site for protein synthesis and degradation?
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What is the term for the space between the nuclear envelope and the plasma membrane?
What is the term for the space between the nuclear envelope and the plasma membrane?
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What is the purpose of biologic membranes surrounding organelles?
What is the purpose of biologic membranes surrounding organelles?
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What happens to newly synthesized proteins without a sorting signal?
What happens to newly synthesized proteins without a sorting signal?
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What is the function of the cytosol as a storage unit?
What is the function of the cytosol as a storage unit?
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What is the role of RNA in directing cellular activities?
What is the role of RNA in directing cellular activities?
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What is the percentage of the cell volume occupied by the cytosol?
What is the percentage of the cell volume occupied by the cytosol?
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What is present in the cytosol involved in intermediate metabolism?
What is present in the cytosol involved in intermediate metabolism?
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What is the purpose of ribosomes in the cytosol?
What is the purpose of ribosomes in the cytosol?
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What is the outer membrane of a eukaryotic cell called?
What is the outer membrane of a eukaryotic cell called?
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What is the function of the nuclear pore complexes (NPCs)?
What is the function of the nuclear pore complexes (NPCs)?
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What is the main function of histones in eukaryotic cells?
What is the main function of histones in eukaryotic cells?
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What is the primary function of the nucleus?
What is the primary function of the nucleus?
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What is the significance of the nuclear envelope being continuous with the membranes of the endoplasmic reticulum?
What is the significance of the nuclear envelope being continuous with the membranes of the endoplasmic reticulum?
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What is the purpose of DNA folding into chromosomes in eukaryotic cells?
What is the purpose of DNA folding into chromosomes in eukaryotic cells?
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What is the role of the nucleolus in the nucleus?
What is the role of the nucleolus in the nucleus?
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What is the result of the advances in microscopy and computer software?
What is the result of the advances in microscopy and computer software?
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What is the significance of understanding the structure and function of eukaryotic cells?
What is the significance of understanding the structure and function of eukaryotic cells?
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What is the overall impact of understanding eukaryotic cells on biology?
What is the overall impact of understanding eukaryotic cells on biology?
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What is the critical process that occurs in the endoplasmic reticulum (ER) that is vital for cellular function?
What is the critical process that occurs in the endoplasmic reticulum (ER) that is vital for cellular function?
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What is the term used to describe the cellular response to the accumulation of unfolded or misfolded proteins in the ER?
What is the term used to describe the cellular response to the accumulation of unfolded or misfolded proteins in the ER?
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What are the membranous structures that bud from the Golgi complex and contain proteins and lipids for secretion or transport to other organelles?
What are the membranous structures that bud from the Golgi complex and contain proteins and lipids for secretion or transport to other organelles?
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What is the type of vesicle that has a coat made largely of the protein clathrin and is involved in the inward endocytic pathway?
What is the type of vesicle that has a coat made largely of the protein clathrin and is involved in the inward endocytic pathway?
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What is the organelle that communicates with the Golgi complex and is involved in protein synthesis, folding, and modification?
What is the organelle that communicates with the Golgi complex and is involved in protein synthesis, folding, and modification?
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What is the process by which the ER responds to the accumulation of misfolded proteins and initiates a stress response?
What is the process by which the ER responds to the accumulation of misfolded proteins and initiates a stress response?
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What is the term used to describe the movement of proteins from the ER to the Golgi complex for further processing and modification?
What is the term used to describe the movement of proteins from the ER to the Golgi complex for further processing and modification?
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What is the function of chaperones in the ER?
What is the function of chaperones in the ER?
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What is the consequence of misfolded proteins not being repaired in the ER?
What is the consequence of misfolded proteins not being repaired in the ER?
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What is the significance of understanding the mechanisms of ER stress and the unfolded-protein response?
What is the significance of understanding the mechanisms of ER stress and the unfolded-protein response?
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What is the main function of ribosomes?
What is the main function of ribosomes?
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What is the role of signal recognition particles (SRPs) in protein synthesis?
What is the role of signal recognition particles (SRPs) in protein synthesis?
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What is the difference between rough and smooth endoplasmic reticulum?
What is the difference between rough and smooth endoplasmic reticulum?
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What is the function of the endoplasmic reticulum?
What is the function of the endoplasmic reticulum?
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What is the significance of the nuclear envelope being continuous with the endoplasmic reticulum?
What is the significance of the nuclear envelope being continuous with the endoplasmic reticulum?
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What is the role of the nucleolus in the nucleus?
What is the role of the nucleolus in the nucleus?
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What is the function of the nuclear pore complexes (NPCs)?
What is the function of the nuclear pore complexes (NPCs)?
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What is the result of the binding of signal recognition particles (SRPs) to the ribosome?
What is the result of the binding of signal recognition particles (SRPs) to the ribosome?
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What is the fate of the newly synthesized protein after it is synthesized by the ribosome?
What is the fate of the newly synthesized protein after it is synthesized by the ribosome?
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What is the significance of the endoplasmic reticulum in sensing cellular stress?
What is the significance of the endoplasmic reticulum in sensing cellular stress?
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What is the primary function of lysosomes in the cell?
What is the primary function of lysosomes in the cell?
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What type of enzymes are found in lysosomes?
What type of enzymes are found in lysosomes?
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At what pH do lysosomal enzymes function optimally?
At what pH do lysosomal enzymes function optimally?
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What is the significance of lysosomes beyond their degradative role?
What is the significance of lysosomes beyond their degradative role?
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What is the outcome of altered lysosomal function?
What is the outcome of altered lysosomal function?
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What is the role of transport proteins in the lysosome membrane?
What is the role of transport proteins in the lysosome membrane?
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What is the result of efficient lysosomal function?
What is the result of efficient lysosomal function?
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What is the relationship between lysosomes and cellular adaptation?
What is the relationship between lysosomes and cellular adaptation?
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What is the significance of lysosomal function in cellular maintenance?
What is the significance of lysosomal function in cellular maintenance?
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How do lysosomes interact with other cellular organelles?
How do lysosomes interact with other cellular organelles?
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What is the purpose of the lysosomal membrane?
What is the purpose of the lysosomal membrane?
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What happens when lysosomal enzymes leak into the cytoplasmic matrix?
What happens when lysosomal enzymes leak into the cytoplasmic matrix?
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What is the name of the disease caused by the lack of lysosomal α-1,4-glucosidase?
What is the name of the disease caused by the lack of lysosomal α-1,4-glucosidase?
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What is the name of the disease caused by the deficiency or absence of lysosomal hexosaminidase A?
What is the name of the disease caused by the deficiency or absence of lysosomal hexosaminidase A?
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What is the term for the uptake of macromolecules from extracellular fluid?
What is the term for the uptake of macromolecules from extracellular fluid?
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What is the term for the engulfment of large particles or microorganisms by phagocytic cells?
What is the term for the engulfment of large particles or microorganisms by phagocytic cells?
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What is the term for the self-digestion process that begins in the cytosol and is used to digest cytosol and ineffectual organelles?
What is the term for the self-digestion process that begins in the cytosol and is used to digest cytosol and ineffectual organelles?
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What is the method by which lysosomes maintain a low internal pH?
What is the method by which lysosomes maintain a low internal pH?
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What is the result of undigested uric acid accumulation within lysosomes in gout?
What is the result of undigested uric acid accumulation within lysosomes in gout?
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What is the term for the membrane-bound vesicle that forms when extracellular substances are taken into the cell?
What is the term for the membrane-bound vesicle that forms when extracellular substances are taken into the cell?
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What is the pH level at which hydrolytic enzymes are maximally active?
What is the pH level at which hydrolytic enzymes are maximally active?
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What is the term for lysosomes that are not active and do not maintain an acidic internal pH?
What is the term for lysosomes that are not active and do not maintain an acidic internal pH?
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What is the process called when lysosomes digest cellular debris or obsolete parts?
What is the process called when lysosomes digest cellular debris or obsolete parts?
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What is the term for lysosomes involved in autophagy?
What is the term for lysosomes involved in autophagy?
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What is the fate of indigestible material in lysosomes?
What is the fate of indigestible material in lysosomes?
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What is the term for the pigmented substance that accumulates in cells as a result of autophagy?
What is the term for the pigmented substance that accumulates in cells as a result of autophagy?
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What is the role of autophagy in cellular function?
What is the role of autophagy in cellular function?
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What happens to the products of autophagy?
What happens to the products of autophagy?
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What is the outcome of defects in autophagy?
What is the outcome of defects in autophagy?
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When do lysosomes become active?
When do lysosomes become active?
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What is the primary function of peroxisomes?
What is the primary function of peroxisomes?
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What is the role of catalase in peroxisomes?
What is the role of catalase in peroxisomes?
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What is the shape and size of peroxisomes compared to lysosomes?
What is the shape and size of peroxisomes compared to lysosomes?
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What is the major site of oxygen utilization in a cell?
What is the major site of oxygen utilization in a cell?
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What is the function of mitochondria in a cell?
What is the function of mitochondria in a cell?
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What is the structure of the mitochondrial inner membrane?
What is the structure of the mitochondrial inner membrane?
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What is the result of impairment of peroxisomes?
What is the result of impairment of peroxisomes?
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What is the role of peroxisomes in the synthesis of specialized phospholipids?
What is the role of peroxisomes in the synthesis of specialized phospholipids?
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What is the function of the enzymes in the mitochondrial inner membrane?
What is the function of the enzymes in the mitochondrial inner membrane?
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What is the purpose of the cristae in the mitochondrial inner membrane?
What is the purpose of the cristae in the mitochondrial inner membrane?
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What percentage of the total cell volume is accounted for by the cytosol?
What percentage of the total cell volume is accounted for by the cytosol?
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What type of biochemical reactions occur in the cytosol?
What type of biochemical reactions occur in the cytosol?
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What is the purpose of ribosomal protein synthesis in the cytosol?
What is the purpose of ribosomal protein synthesis in the cytosol?
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What is the term for the process of synthesizing, degrading, and transforming small organic molecules in the cytosol?
What is the term for the process of synthesizing, degrading, and transforming small organic molecules in the cytosol?
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What type of molecules are stored in the cytosol as glycogen?
What type of molecules are stored in the cytosol as glycogen?
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What is the function of secretory vesicles in the cytosol?
What is the function of secretory vesicles in the cytosol?
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What are the clusters of ribosomes in the cytosol called that synthesize identical proteins?
What are the clusters of ribosomes in the cytosol called that synthesize identical proteins?
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What is the site of protein synthesis in the cytosol?
What is the site of protein synthesis in the cytosol?
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What is the purpose of the cytosol in terms of cellular activities?
What is the purpose of the cytosol in terms of cellular activities?
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What is the primary function of microtubules in the cell?
What is the primary function of microtubules in the cell?
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What is mechanotransduction, and what is its significance in cells?
What is mechanotransduction, and what is its significance in cells?
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What is the function of cilia on cells lining the respiratory tract?
What is the function of cilia on cells lining the respiratory tract?
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What are the three main types of filaments that make up the cytoskeleton?
What are the three main types of filaments that make up the cytoskeleton?
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What is the arrangement of microtubules in a centriole?
What is the arrangement of microtubules in a centriole?
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How do cells sense their physical environment?
How do cells sense their physical environment?
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Where are actin filaments usually concentrated in the cell?
Where are actin filaments usually concentrated in the cell?
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What is the significance of the cytoskeleton in eukaryotic cells?
What is the significance of the cytoskeleton in eukaryotic cells?
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What is the significance of intermediate filaments in epithelial tissue?
What is the significance of intermediate filaments in epithelial tissue?
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What is the role of microtubules in the external movement or motility of some cells?
What is the role of microtubules in the external movement or motility of some cells?
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What is the term used to describe the arrangement of microfilaments in motile cells?
What is the term used to describe the arrangement of microfilaments in motile cells?
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What is the term used to describe the cellular processes that translate mechanical stimuli into biochemical signals?
What is the term used to describe the cellular processes that translate mechanical stimuli into biochemical signals?
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What is the function of microtubules during cellular division?
What is the function of microtubules during cellular division?
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What is the significance of the cytoskeleton in maintaining cell shape and internal organization?
What is the significance of the cytoskeleton in maintaining cell shape and internal organization?
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What is the role of actin filaments in cell junctions?
What is the role of actin filaments in cell junctions?
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What are the consequences of cell stresses that involve adaptations of mechanotransduction?
What are the consequences of cell stresses that involve adaptations of mechanotransduction?
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What are the three main components of the cytoskeleton?
What are the three main components of the cytoskeleton?
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What is the significance of the nuclear lamina?
What is the significance of the nuclear lamina?
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What is the function of microtubules in flagella?
What is the function of microtubules in flagella?
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What is the main component of the cortical actin network?
What is the main component of the cortical actin network?
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What is the main function of phospholipids in cell membranes?
What is the main function of phospholipids in cell membranes?
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What is the term for the temporary, dynamic formations of domains in the plasma membrane?
What is the term for the temporary, dynamic formations of domains in the plasma membrane?
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What is the function of inositol phospholipids in cell signaling?
What is the function of inositol phospholipids in cell signaling?
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What is the purpose of the hydrophilic region of a lipid molecule?
What is the purpose of the hydrophilic region of a lipid molecule?
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What is the result of the bilayer structure of the plasma membrane?
What is the result of the bilayer structure of the plasma membrane?
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What is the function of caveolae in cell communication?
What is the function of caveolae in cell communication?
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What is the main component of cell membranes in animals?
What is the main component of cell membranes in animals?
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What is the characteristic of lipid molecules that allows them to form a bilayer?
What is the characteristic of lipid molecules that allows them to form a bilayer?
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What is the result of the fluidity of the plasma membrane?
What is the result of the fluidity of the plasma membrane?
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What is the function of lipids along with protein assemblies in the plasma membrane?
What is the function of lipids along with protein assemblies in the plasma membrane?
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What is the main function of cell membranes in controlling the composition of the space they enclose?
What is the main function of cell membranes in controlling the composition of the space they enclose?
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How do polarized domains contribute to directional transport across cell membranes?
How do polarized domains contribute to directional transport across cell membranes?
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What is the role of protein receptors in cell-to-cell recognition?
What is the role of protein receptors in cell-to-cell recognition?
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What is the basic structure of cell membranes?
What is the basic structure of cell membranes?
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What is the function of membrane proteins in cell membranes?
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What is the significance of cell polarity in maintaining normal cell and tissue structure?
What is the significance of cell polarity in maintaining normal cell and tissue structure?
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What is the role of the plasma membrane in cellular mobility and maintenance of cellular shape?
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What is the significance of the lipid bilayer in cell membranes?
What is the significance of the lipid bilayer in cell membranes?
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How do individual lipid molecules move within the lipid bilayer?
How do individual lipid molecules move within the lipid bilayer?
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What is the overall function of cell membranes in controlling metabolic pathways?
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What is the primary function of proteins in facilitating transport across membranes?
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What is the state of cell balance referred to as in terms of protein synthesis, folding, and degradation?
What is the state of cell balance referred to as in terms of protein synthesis, folding, and degradation?
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What is the function of molecular chaperones called heat-shock proteins (hsp) in protein homeostasis?
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What is the outcome when cells lack a sorting signal for protein transport?
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What is the role of vesicles in protein transport?
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What is the significance of understanding protein trafficking and misfolded proteins in disease?
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What is the function of the proteostasis network in regulating protein homeostasis?
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What is the role of proteins in maintaining cellular shape and adhesion?
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What is the outcome when the proteostasis network fails or malfunctions?
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What is the role of proteins in energy transduction across the plasma membrane?
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How do membrane proteins associate with the lipid bilayer?
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What is the significance of the amino acid sequence in protein synthesis?
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What are the three ways proteins move from one compartment to another?
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What is the function of nuclear pore complexes in gated transport?
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What is the characteristic feature of membrane proteins?
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How do proteins fold into their functional configurations?
How do proteins fold into their functional configurations?
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What is the role of ribosomes in protein synthesis?
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What is the significance of the unique sequence of amino acids in proteins?
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What is the function of the cytosol in protein synthesis?
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What is the significance of the interaction between proteins and the lipid bilayer?
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What is the primary function of proteases in the human body?
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What is the term used to describe the carbohydrate coating on the outside of the plasma membrane?
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What are the four major proteolytic cascades with disease relevance?
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What is the function of the carbohydrate coating on the cell surface?
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What is the role of glycoproteins, proteoglycans, and glycolipids in the cell membrane?
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What is the significance of dysregulation of proteases in human diseases?
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What is the purpose of the glycocalyx in terms of cell-cell interaction?
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What is the function of proteases in the proteolytic cascade?
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What is the outcome of the dysregulation of proteases in the proteolytic cascade?
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What is the significance of the glycocalyx in terms of cell protection?
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What is the function of lectins in recognizing neutrophils at the site of bacterial infection?
What is the function of lectins in recognizing neutrophils at the site of bacterial infection?
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What is the binding site of a protein receptor, and how does it interact with a ligand?
What is the binding site of a protein receptor, and how does it interact with a ligand?
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What is the significance of plasma membrane receptors in determining cellular response to ligands?
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How do receptors for endorphins and opiates modulate pain perception?
How do receptors for endorphins and opiates modulate pain perception?
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What is the function of antigen receptors on white blood cells?
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What is the outcome of ligand binding to a receptor, and how is it regulated?
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What is the role of hormone binding in regulating protein synthesis within the cell?
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What is the significance of membrane receptors in the regulation of cellular function?
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What is the outcome of receptor binding to a ligand, and how does it influence cellular response?
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What is the significance of receptor classification based on location and function?
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What is the main purpose of the extracellular matrix in cells?
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What is the difference between the extracellular matrix and the basement membrane?
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How do cells adhere to each other and form tissues?
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What is the primary function of the plasma membrane in cells?
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What is the primary function of collagen in the extracellular matrix?
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How do cells lift heavy objects like a textbook?
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Study Notes
Cellular Biology
- Understanding cellular biology is crucial for understanding disease
- Cellular biology is integral to understanding how cells behave as a multicellular organism
- Cellular communication (cellular crosstalk) is at the heart of cellular biology
- Cellular crosstalk involves the origination, transmission, reception, interpretation, and utilization of messages between, among, and within cells
- This streamlined conversation maintains cellular function and specialization
- Intercellular signals enable each cell to determine its position and specialized role
Cellular Function and Disease
- Cells must demonstrate a "chemical fondness" for other cells and their surrounding environment to maintain the integrity of the entire organism
- When cells no longer tolerate this fondness, the conversation breaks down
- Breakdown of cellular communication can lead to cells either adapting (sometimes altering function) or becoming vulnerable to:
- Isolation
- Injury
- Disease
- Death
Cell Classification
- Living cells are divided into two major classes: eukaryotes and prokaryotes.
- Eukaryotes include cells of higher animals and plants, fungi, protozoa, and most algae.
- Prokaryotes include cyanobacteria, bacteria, and rickettsiae.
Eukaryotic Cell Characteristics
- Eukaryotes are larger and have more extensive intracellular anatomy and organization than prokaryotes.
- Eukaryotic cells have a characteristic set of membrane-bound intracellular compartments called organelles.
- Organelles include a well-defined nucleus.
- Eukaryotic cells have multiple chromosomes.
Prokaryotic Cell Characteristics
- Prokaryotes contain no organelles.
- Prokaryotic cells lack a distinct nucleus.
- The nuclear material is not encased by a nuclear membrane.
- Prokaryotes have a single circular chromosome.
- Prokaryotes lack histones, which are proteins that bind with DNA and are involved in the supercoiling of DNA.
Cellular Differences
- Chemical composition and biochemical activity differ between prokaryotic and eukaryotic cells.
- Protein production (synthesis) differs between the two classes of cells due to major structural differences in RNA-protein complexes.
- Mechanisms of transport across the outer cellular membrane differ between prokaryotic and eukaryotic cells.
- Enzyme content differs between prokaryotic and eukaryotic cells.
Cellular Specialization
- Cells become specialized through differentiation, allowing them to perform specific functions.
- Specialized cells often lack certain properties, but excel in others.
Eight Chief Cellular Functions
Movement
- Muscle cells generate forces to produce motion.
- Examples: muscle cells attached to bones produce limb movements, and smooth muscle cells surrounding blood vessels change diameter.
Conductivity
- Conduction is a response to a stimulus, manifesting as a wave of excitation.
- Nerve cells primarily function for conductivity.
Metabolic Absorption
- Cells take in and use nutrients and substances from their surroundings.
- Examples: intestinal cells reabsorb fluids and synthesize protein enzymes, and kidney cells reabsorb fluids and synthesize proteins.
Secretion
- Certain cells synthesize new substances and secrete them as needed.
- Examples: mucous gland cells, adrenal gland cells, and ovary cells secrete hormonal steroids.
Excretion
- Cells rid themselves of waste products through membrane-bound sacs (lysosomes) containing enzymes.
- Lysosomes break down large molecules into waste products released from the cell.
Respiration
- Cells absorb oxygen to transform nutrients into energy in the form of adenosine triphosphate (ATP).
- Cellular respiration occurs in mitochondria.
Reproduction
- Tissue growth occurs through cell enlargement and reproduction.
- Cellular division is necessary for tissue maintenance and replacement.
Communication
- Communication is vital for cells to survive as a society.
- Examples: pancreatic cells secrete insulin to signal muscle cells to absorb sugar from the blood.
Eukaryotic Cell Structure
- A eukaryotic cell consists of three components: plasma membrane, cytoplasm, and organelles.
- The plasma membrane, also known as the plasmalemma, is the outer membrane of the cell.
Nucleus
- The nucleus is the largest membrane-bound organelle, located in the center of the cell, surrounded by cytoplasm.
- The nuclear envelope is composed of two pliable membranes with nuclear pores, allowing molecules to move between the nucleus and cytosol.
- The nucleus contains the nucleolus, cellular DNA, and histone proteins that regulate DNA activity.
- Histones binding to DNA causes DNA to fold into chromosomes, which is essential for cell division in eukaryotes.
Functions of the Nucleus
- The primary functions of the nucleus are cell division and control of genetic information.
- The nucleus is also responsible for DNA replication and repair, and transcription of genetic information into RNA.
- RNA is processed into messenger, transport, and ribosomal RNA and introduced into the cytoplasm, where it directs cellular activities.
Cytoplasm
- Cytoplasm is an aqueous solution (cytosol) that fills the space between the nuclear envelope and the plasma membrane.
- Cytosol represents about half the volume of a eukaryotic cell and contains thousands of enzymes involved in intermediate metabolism.
- Cytosol is the main site for protein synthesis and degradation, and is crowded with ribosomes that make proteins.
Organelles
- Organelles suspended in the cytoplasm are enclosed in biologic membranes, enabling them to carry out functions that require different biochemical environments.
- Organelle functions include protein synthesis and transport, hormone synthesis, waste elimination, metabolic processes, and maintenance of cellular structure and motility.
Ribosomes
- Ribosomes are RNA-protein complexes synthesized in the nucleolus and secreted into the cytoplasm through nuclear pore complexes (NPCs)
- They can float free in the cytoplasm or attach to the outer membranes of the endoplasmic reticulum (ER)
- Their chief function is to provide sites for cellular protein synthesis
- Newly formed ribosomes synthesize a “recognition sequence,” or signal, like an address on a letter
- Signal recognition particles (SRPs) in the cytosol bind to the ribosome after recognizing the SRP
- Ribophorins, receiver proteins found on the rough sections of the ER, act as the “address” site or binding site
- The developing protein threads its way through the ER membrane into the lumen
- The SRP is removed and the new protein chain is folded into its final conformation
Endoplasmic Reticulum (ER)
- The ER is a membrane factory that specializes in the synthesis and transport of protein and lipid components of most of the cell's organelles
- It consists of a network of tubular or saclike channels (cisternae) that extend throughout the cytoplasm and are continuous with the outer nuclear membrane
- The ER may be rough (granular) or smooth (agranular)
- Rough ER (rER) is rough because ribosomes and ribonucleoprotein particles are attached to it
- Some of the proteins synthesized by these ribosomes remain in the ER, and others are used to construct membranes of other organelles
- The ER is responsible for much of a cell's protein synthesis and folding, and senses cellular stress
Protein Folding
- Protein folding in the ER is critical for us
- Proteins must fold into complex three-dimensional structures to perform vital functions
- Most secreted proteins fold and are modified in an error-free manner, but ER or cell stress, mutations, or random errors during protein synthesis can decrease the folding amount or rate
- Pathophysiologic processes, environmental toxins, and mutant protein expression can perturb the sensitive ER environment
- The ER has protective ways to help folding, such as protein chaperones
- Misfolded proteins not repaired in the ER can lead to ER stress and initiate apoptosis or cell death
Unfolded-Protein Response (UPR)
- The ER mediates intracellular signaling pathways in response to the accumulation of unfolded or misfolded proteins
- The UPR is an adaptive response to ER stress
- Investigators are studying UPR-associated inflammation and how the UPR is coupled to inflammation in health and disease
- Specific diseases associated with ER stress include Alzheimer disease, Parkinson disease, prion disease, amyotrophic lateral sclerosis, diabetes mellitus, and sepsis
Smooth Endoplasmic Reticulum (sER)
- sER does not contain ribosomes or ribonucleoprotein particles
- Membranous surfaces of the sER contain enzymes involved in the synthesis of steroid hormones
- sER is responsible for a variety of reactions required to remove toxic substances from the cell
Golgi Complex
- The Golgi complex is a network of flattened, smooth membranes and vesicles frequently located near the nucleus of the cell
- Proteins from the ER are processed and packaged into small membrane-bound sacs or vesicles called secretory vesicles
- Secretory vesicles collect at the end of the membranous folds of the Golgi bodies—called cisternae
- The vesicles then break off from the Golgi complex and migrate to a variety of intracellular and extracellular destinations, including the plasma membrane
- The vesicles fuse with the plasma membrane, and their contents are released from the cell
Lysosomes: Structure and Function
- Lysosomes are membrane-enclosed organelles that contain over 60 digestive enzymes (hydrolases) that break down macromolecules, defunct organelles, and external particles engulfed by endocytosis.
- Hydrolases function optimally at an acidic pH, catalyzing bonds in proteins, lipids, nucleic acids, and carbohydrates.
Intracellular Digestive System
- Lysosomes act as the intracellular digestive system, capable of breaking down cellular constituents to their basic components (amino acids, nucleotides, and carbohydrates).
- Transport proteins in the lysosome membrane carry these components to the cytosol, where the cell can reuse or excrete them.
Signaling Function
- Lysosomes have a newly discovered signaling function, cooperating with their degradative role to mediate basic cellular functions (nutrient-dependent signal transduction for cellular adaptation).
- Complex transcriptional programs control lysosome synthesis, composition, and quantity, regulating their activity to match cellular needs.
Cellular Health and Disease
- Lysosomes maintain cellular health by efficiently removing toxic cellular components, useless organelles, and terminating signal transduction.
- Alterations in lysosomal function are central to the pathophysiology of various conditions, including storage diseases, neurodegenerative diseases, and cancer.
- Aging leads to a progressive loss of lysosomal efficiency and decline of regenerative capacity in organs and tissues.
Lysosomes as Signaling Hubs
- Lysosomes are key signaling hubs, integrating functions such as nutrient abundance, energy levels, and cell stressors to regulate cellular metabolism.
- The signaling functions have far-reaching implications for metabolic regulation in health and disease.
Lysosomal Function
- Lysosomal membrane acts as a protective shield between the powerful digestive enzymes within the lysosome and the cytoplasm, preventing their leakage into the cytoplasmic matrix.
- Disruption of the membrane leads to a release of the lysosomal enzymes, causing cellular self-digestion.
Lysosomal Abnormalities
- Lysosomal abnormalities are involved in various conditions that involve cellular injury and death.
- Lysosomal storage diseases (LSDs) can result from a genetic defect or lack of one or more lysosomal enzymes.
Examples of Lysosomal Storage Diseases
- Pompe disease: lack of lysosomal α-1,4-glucosidase leads to an accumulation of glycogen in lysosomes.
- Tay-Sachs disease: deficiency or absence of lysosomal hexosaminidase A leads to an accumulation of GM2 ganglioside in lysosomes.
- Gout: undigested uric acid accumulates within lysosomes, damaging the lysosomal membrane.
Pathways of Degradation in Lysosomes
- Four pathways: endocytosis, phagocytosis, macropinocytosis, and autophagy.
- Endocytosis: uptake of macromolecules from extracellular fluid.
- Phagocytosis: engulfment of large particles or microorganisms in phagocytic cells.
- Macropinocytosis: nonspecific uptake of fluids, membrane, and particles attached to the plasma membrane.
- Autophagy: self-eating, begins in the cytosol, used to digest cytosol and ineffectual organelles.
Lysosomal Activation
- Lysosomes remain fully active by maintaining a low internal pH.
- Hydrolytic enzymes are only maximally active at acidic pH values.
- Primary lysosomes: not active, do not maintain acidic internal pH.
- Secondary lysosomes (or heterophagosomes): formed when primary lysosome fuses with a vacuole or other organelle, pH decreases, and hydrolytic enzymes become activated.
Autophagy and Autolysosomes
- Autophagy: promotes homeostasis, involves continuous biosynthesis and cell turnover, plays a crucial role in health.
- Autolysosomes (or autophagosomes): involved in autophagy, digest cellular debris and obsolete parts.
- Defects in autophagy may challenge disposal mechanisms, contributing to disease.
Autophagy Products
- Products of autophagy (and phagocytosis) pass out of the lysosome and are reused by the cell.
- Indigestible material is stored in residual bodies, whose contents are actively expelled from the cell.
- High concentrations of lipids may accumulate within residual bodies, eventually forming lipofuscin, a pigmented substance.
Peroxisomes
- Peroxisomes are membrane-bound organelles containing oxidative enzymes like catalase and urate oxidase.
- These enzymes detoxify compounds and fatty acids by removing hydrogen atoms from specific substrates, producing hydrogen peroxide (H2O2).
- Peroxisomes are larger and oval or irregular in shape, similar to lysosomes in microscopic appearance.
- They are major sites of oxygen utilization, similar to mitochondria.
- Catalase, an antioxidant enzyme, uses H2O2 to oxidize various substrates, breaking down H2O2 into H2O and O2.
- Peroxisomes play a crucial role in synthesizing specialized phospholipids necessary for nerve cell myelination.
- Impairment of peroxisomes can lead to disease.
Mitochondria
- Mitochondria are organelles found in large numbers in most cells, responsible for cellular respiration and energy production.
- They appear as spheres, rods, or filamentous bodies bound by a double membrane.
- The outer membrane is smooth, while the inner membrane is convoluted in the mitochondrial matrix to form cristae.
- The inner membrane contains enzymes of the respiratory chain, essential for oxidative phosphorylation and generating most of the cell's ATP.
- Metabolic pathways involved in carbohydrate, lipid, and amino acid metabolism, as well as urea and heme synthesis, are located in the mitochondrial matrix.
- The outer membrane is permeable to many substances, while the inner membrane is highly selective and contains transmembranous transport systems.
- Mitochondria contain their own DNA, which codes for enzymes needed for oxidative phosphorylation.
Cytosol Structure and Function
- Cytosol is the gelatinous, semiliquid portion of the cytoplasm, making up about 55% of the total cell volume.
Intermediary Metabolism
- Intermediary metabolism involves the synthesis, degradation, and transformation of small organic molecules (e.g., simple sugars, fatty acids, and amino acids).
- These reactions occur in the cytoplasm, with most of the metabolism taking place in the cytosol.
- Intermediary metabolism enables energy to be used for managing cellular activities and for providing substrates to maintain cell integrity.
Protein Synthesis
- Ribosomal protein synthesis takes place in free ribosomes in the cytosol.
- Cytosolic ribosomes that synthesize identical proteins are collected in "factories" known as polyribosomes.
Storage and Transport
- Excess stored nutrients not immediately used for ATP production are converted in the cytosol into storage forms (e.g., excess glucose is stored as glycogen).
- These temporary storage forms are known as inclusions.
- Secretory vesicles that have been processed and packaged by the endoplasmic reticulum and Golgi complex remain in the cytosol.
- These vesicles transport and empty their contents outside the cell through signaling.
Cytoskeleton
- The cytoskeleton is a network of protein filaments that provides the "bones and muscles" of the cell, maintaining cell shape and internal organization, and permitting movement of substances within the cell and external projections.
- The cytoskeleton is composed of three main types of filaments: actin filaments, microtubules, and intermediate filaments.
Mechanotransduction
- Mechanotransduction is the process by which cells translate mechanical stimuli into biochemical signals, allowing cells to adapt to their surroundings.
- Cell stresses involved in mechanotransduction are associated with several alterations and diseases, including loss of hearing, cardiovascular disease, muscular dystrophy, and cancer.
Microtubules
- Microtubules are small, hollow, cylindrical, unbranched tubules made of protein that add strength to the cell's structure.
- Microtubules support and move organelles within the cell, facilitate transport of impulses along nerve cells, and have roles in inflammatory and immune responses and hormone secretion.
- Microtubules are also involved in external movement, or motility, of some cells, such as the movement of sperm cells and cilia on cells lining the respiratory tract.
Centrioles and Cellular Division
- During cellular division (mitosis), microtubules involved in cellular division are arranged in a centriole, which consists of nine bundles containing three microtubules each.
- The pairs of centrioles split and migrate to opposite poles of the cell during division.
Actin Filaments (Microfilaments)
- Actin filaments are smaller fibrils that occur in bundles, concentrated in the cell cortex just beneath the plasma membrane.
- Actin filaments link the interior of the cell to adjacent cells through cell junctions and are necessary for regulating cell growth and driving cellular locomotion.
Intermediate Filaments
- Intermediate filaments are braided, ropelike fibers made of several filament proteins that form a mesh called the nuclear lamina beneath the inner nuclear membrane, creating a protective chamber for the cell's DNA.
- Intermediate filaments also crisscross the cytoplasm, promoting mechanical strength, and in epithelial tissue, they bridge the cytoplasm from one cell junction to another, supporting and strengthening the sheet of epithelium.
Cell Membrane Structure and Function
- Membranes define cell boundaries and control the composition of the compartment they enclose by allowing or excluding various molecules.
- Membranes exert a powerful influence on metabolic pathways by controlling the movement of substances from one compartment to another.
Cell Polarity and Transport
- Directional transport is facilitated by polarized domains, distinct apical and basolateral domains.
- Cell polarity maintains normal cell and tissue structure, importantly for transport of nutrients in and out of the cell.
Plasma Membrane Functions
- The plasma membrane has an important role in cell-to-cell recognition, with protein receptors for hormones and chemical signals acting as markers.
- The plasma membrane assists with cellular mobility and maintenance of cellular shape.
Cell Membrane Components
- The main components of cell membranes are lipids and proteins.
- The basic structure of cell membranes is the lipid bilayer, composed of two continuous opposing leaflets and proteins that span the bilayer.
Lipid Bilayer Structure and Function
- The lipid bilayer provides the basic fluid structure of the membrane and is mostly an impermeable barrier to water-soluble molecules.
- Individual lipid molecules can diffuse readily throughout their own monolayer.
Lipid Rafts and Domains
- Lipids and proteins are not distributed uniformly but are seen to concentrate in a temporary, dynamic way assisted by protein-protein interactions.
- Lipid rafts in living cells may be important for cell communication, where protein assemblies convert extracellular signals into intracellular signals.
Phospholipids and Lipid Classes
- Phospholipids are key for repairing the membrane and tend to spontaneously rearrange themselves to avoid a tear.
- Inositol phospholipids are a subclass of phospholipids important for cell signaling because they respond to extracellular signals in the cytosolic lipid leaflet of the bilayer.
Amphipathic Molecules and Bilayer Structure
- An amphipathic molecule has a hydrophobic (uncharged) part and a hydrophilic (charged) part.
- The membrane spontaneously organizes itself into a bilayer because of these two incompatible solubilities.
Bilayer Function and Permeability
- The bilayer is impermeable to most water-soluble molecules because they are insoluble in the oily core region.
- The bilayer serves as a barrier to the diffusion of water and hydrophilic substances while allowing lipid-soluble molecules to diffuse through it readily.
Proteins in the Plasma Membrane
- Proteins perform most of the plasma membrane's specific tasks, with varying amounts and types of proteins in a membrane.
- Proteins are made from a chain of amino acids, known as polypeptides, with 20 types of amino acids in proteins, and each type of protein has a unique sequence of amino acids.
Association of Proteins with the Lipid Bilayer
- Membrane proteins associate with the lipid bilayer in different ways, including:
- Transmembrane proteins extending across the bilayer and exposed to an aqueous environment on both sides.
- Proteins located almost entirely in the cytosol, associated with the cytosolic half of the lipid bilayer by an α helix exposed on the surface of the protein.
- Proteins existing outside the bilayer, on one side or the other, and attached to the membrane by one or more covalently attached lipid groups.
- Proteins bound indirectly to one or the other bilayer membrane face and held in place by their interactions with other proteins.
Properties of Membrane Proteins
- Membrane proteins are amphiphilic, with both hydrophobic and hydrophilic regions.
- Proteins exist in densely folded molecular configurations, with an excess of hydrophilic units at the surface of the molecule and an excess of hydrophobic units inside.
Synthesis and Trafficking of Membrane Proteins
- Membrane proteins are synthesized mainly by ribosomes in the cytosol and then travel to their destinations.
- The fate of a protein depends on its amino acid sequence, which contains sorting signals that direct its delivery to locations outside the cytosol or to organelle surfaces.
- Proteins move from one compartment to another by gated transport, protein translocation, or vesicular transport.
Functions of Membrane Proteins
- Proteins facilitate transport across membranes by serving as:
- Receptors for substances moving in and out of the cell.
- Pores or transport channels for ions, electrolytes, amino acids, and monosaccharides.
- Enzymes that drive active pumps to promote concentration of certain ions within the cell.
- Cell surface markers, such as glycoproteins, that identify a cell to its neighbor.
- Cell adhesion molecules that allow cells to hook together and form attachments to the cytoskeleton for maintaining cellular shape.
- Catalysts of chemical reactions, such as the conversion of lactose to glucose.
Proteostasis and Cellular Health
- Proteostasis is a state of cell balance of the processes of protein synthesis, folding, and degradation, which is vital to cellular health.
- The cellular protein pool is in constant change or flux, depending on how quickly proteins are made and how long they survive or are broken down.
- The proteostasis network, comprising ribosomes, chaperones, and two protein breakdown systems (lysosomes and the ubiquitin-proteasome system), regulates protein homeostasis under various conditions.
- Malfunction or failure of the proteostasis network is associated with human disease.
Proteases and Proteolytic Cascades
- Proteases are enzymes that break down proteins and can be tethered to cell membranes.
- Proteases participate in a tightly orchestrated sequence of events called a proteolytic cascade, which regulates essential physiological processes.
- Four major proteolytic cascades with disease relevance are cell death or caspase-mediated apoptosis, blood coagulation cascade, degrading membrane enzymes or matrix metalloproteinase cascade, and the complement cascade.
- Proteases within a proteolytic cascade can act as initiators, amplifiers, propagators, or executioners.
Cell Surface Carbohydrates
- Cell surface carbohydrates are short chains of sugars or oligosaccharides attached to membrane proteins (glycoproteins) and lipids (glycolipids).
- Long polysaccharide chains attached to membrane proteins are called proteoglycans.
- The carbohydrate coating on the outside of the plasma membrane is called the glycocalyx or cell coat, which helps protect the cell from mechanical damage and provides a slimy surface for cell mobility.
Cellular Recognition and Adhesion
- The glycocalyx facilitates cell-cell recognition and adhesion.
- Intercellular recognition is possible through membrane oligosaccharides that bind to specific lectins, which recognize neutrophils at the site of bacterial infection.
Cellular Receptors
- Cellular receptors are protein molecules that recognize and bind to specific smaller molecules called ligands.
- Receptors can be found on the plasma membrane, in the cytoplasm, or in the nucleus.
- Binding of ligands to receptors depends on the chemical configuration of the receptor and its ligand, and involves weak, noncovalent interactions.
Receptor Function
- Binding of ligands to receptors activates or inhibits the receptor's associated signaling or biochemical pathway.
- Receptors determine which ligands a cell will bind with and how the cell will respond to binding.
- Examples of ligands include hormones and neurotransmitters.
Cell Membrane and Adhesions
- Cells are enclosed by a flimsy membrane, but they can combine strongly to form tissues and organs.
- Plasma membranes allow cells to be held together robustly through cell-to-cell adhesions.
Extracellular Matrix (ECM)
- ECM is an intricate meshwork of interstitial fibrous proteins embedded in a watery, gel-like substance composed of complex carbohydrates.
- ECM is secreted by fibroblasts and provides a pathway for diffusion of nutrients, wastes, and other water-soluble traffic between the blood and tissue cells.
- ECM helps regulate cell growth, movement, and differentiation, and has four major functions: mechanical support, control of cell proliferation, formation of a scaffold for tissue regeneration, and establishment of tissue microenvironments.
Components of ECM
- Fibrous structural proteins: collagen and elastin
- Adhesive glycoproteins: fibronectin
- Proteoglycans and hyaluronic acid
Functions of ECM Components
- Collagen: provides tensile strength or resistance to longitudinal stress
- Elastin: provides elasticity to tissues that must be capable of stretching and recoiling
- Fibronectin: promotes cell adhesion and cell anchorage
Connective Tissue
- Connective tissue is composed of ECM and the cells that produce it (e.g., fibroblasts)
- Connective tissue connects cells together to form tissues and organs
- Examples of human connective tissues: bone, tendons, dermis of the skin, cartilage, and the jelly-like substance that fills the eye.
Cell Adhesion Molecules (CAMs)
- CAMs are cell surface proteins that bind cells to adjacent cells and components of the extracellular matrix (ECM)
- There are four protein families of CAMs: integrins, cadherins, selectins, and immunoglobulin (Ig) superfamily
Integrins
- A major class of receptors within the ECM
- Regulate cell-ECM interactions with collagen, fibronectin, vitronectin, and fibrinogen
Cadherins
- Ca++-dependent glycoproteins with unique tissue distribution patterns
- Example: epithelial (E cadherin)
Selectins
- Bind certain carbohydrates, such as mucins
Immunoglobulin Superfamily (IgSF) CAMs
- Bind integrins or other IgSF CAMs
Cell Junctions
- Specialized regions of plasma membranes that link cells together
- Classified by function:
- Tight junctions: hold cells together, forming a tight seal
- Adherens junctions, desmosomes, and hemidesmosomes: provide strong mechanical attachments
- Gap junctions: allow chemical communication between cells
- Maintain apico-basal polarity of individual epithelial cells (tight junctions)
Classification of Cell Junctions
- Symmetrical junctions: tight junctions (zonula occludens), belt desmosome (zonula adherens), desmosomes (macula adherens), and gap junctions
- Asymmetrical junction: hemidesmosome
Functions of Cell Junctions
- Make the epithelium leak-proof by mediating mechanical attachment of one cell to another
- Allow communicating tunnels between cells
- Maintain cell polarity
Cell Communication
- Cells communicate to maintain homeostasis, regulate growth and division, and coordinate functions, using hundreds of signal molecules.
- Three main ways of cell communication:
- Displaying plasma membrane-bound signaling molecules (receptors) for direct physical contact.
- Affecting receptor proteins inside target cells, requiring signal molecules to enter the cell.
- Forming protein channels (gap junctions) for direct coordination between adjacent cells.
Intercellular Signaling Modes
- Primary modes of intercellular signaling:
- Contact-dependent signaling: requires close membrane-membrane contact.
- Paracrine signaling: local chemical mediators are quickly absorbed, destroyed, or immobilized.
- Hormonal signaling: specialized endocrine cells secrete hormones that travel through tissue and bloodstream.
- Neurohormonal signaling: hormones are released by neurosecretory neurons.
- Neurotransmitter signaling: neurons release short-range neurotransmitters into a small, discrete space (synapse).
Signal Transduction
- Signal transduction involves incoming signals from extracellular chemical messengers (ligands) conveyed to the cell's interior for execution.
- Cells respond to external stimuli by activating signal transduction pathways or signaling cascades.
- Signals are passed between cells through receptor proteins that recognize and respond specifically to signal molecules.
Functions of Signal Transduction Pathways
- Physically transfer the signal from the reception site to another part of the cell.
- Amplify the signal received through a multiplying effect.
- Distribute the signal to influence several processes in parallel, creating branches in the flow.
- Modulate the signal by interfering factors inside or outside the cell.
Responses to Signaling
- Two general responses to binding of extracellular signaling messengers (ligands) to membrane receptors:
- Opening or closing specific channels in the membrane to regulate ion movement.
- Transferring the signal to an intracellular messenger (second messenger), triggering a cascade of biochemical events within the cell.
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Description
Explore the fundamental principles of cellular biology, focusing on cellular communication and its role in understanding disease. Learn how cells interact and respond to messages in a multicellular organism.