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Questions and Answers
What is the primary distinction between diffusion and active transport?
What is the primary distinction between diffusion and active transport?
Diffusion involves the passive movement of substances through the membrane, while active transport requires energy to carry substances against their concentration gradient.
Explain the processes of pinocytosis and phagocytosis.
Explain the processes of pinocytosis and phagocytosis.
Pinocytosis is the ingestion of minute particles and extracellular fluid, while phagocytosis involves the ingestion of large particles like bacteria and dead cells.
How do substances like vitamin B12 and iron enter cells?
How do substances like vitamin B12 and iron enter cells?
Vitamin B12 and iron combine with specific proteins and enter cells via endocytosis through receptor-mediated mechanisms.
What cellular mechanisms are involved in phagocytosis initiation?
What cellular mechanisms are involved in phagocytosis initiation?
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Why is pinocytosis especially rapid in macrophages?
Why is pinocytosis especially rapid in macrophages?
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What role do protein structures play in active transport across cell membranes?
What role do protein structures play in active transport across cell membranes?
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In what way does endocytosis facilitate the entry of large or lipid-insoluble chemicals into cells?
In what way does endocytosis facilitate the entry of large or lipid-insoluble chemicals into cells?
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What percentage of the total macrophage membrane is typically engulfed via pinocytosis each minute?
What percentage of the total macrophage membrane is typically engulfed via pinocytosis each minute?
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What does Fick's law predict in terms of molecular movement?
What does Fick's law predict in terms of molecular movement?
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Explain the role of macromolecules in carrier-mediated diffusion.
Explain the role of macromolecules in carrier-mediated diffusion.
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How does carrier-mediated diffusion differ from Fick's law?
How does carrier-mediated diffusion differ from Fick's law?
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What is the significance of selective inhibitors in transport mechanisms?
What is the significance of selective inhibitors in transport mechanisms?
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Describe facilitated diffusion and its requirements.
Describe facilitated diffusion and its requirements.
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What distinguishes active transport from passive transport processes?
What distinguishes active transport from passive transport processes?
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How does osmosis function in terms of water movement?
How does osmosis function in terms of water movement?
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Why is it essential for cells to obtain nutrients from surrounding fluids?
Why is it essential for cells to obtain nutrients from surrounding fluids?
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What is the process called whereby antibodies facilitate the phagocytosis of pathogens?
What is the process called whereby antibodies facilitate the phagocytosis of pathogens?
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List the first step that occurs during phagocytosis.
List the first step that occurs during phagocytosis.
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What role do actin and contractile fibrils play in phagocytosis?
What role do actin and contractile fibrils play in phagocytosis?
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What substance do lysosomes release into the phagocytic vesicle to aid digestion?
What substance do lysosomes release into the phagocytic vesicle to aid digestion?
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Name two bactericidal agents found in lysosomes that help eliminate phagocytized bacteria.
Name two bactericidal agents found in lysosomes that help eliminate phagocytized bacteria.
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What happens to the indigestible substances remaining in a digestive vesicle?
What happens to the indigestible substances remaining in a digestive vesicle?
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What are the foodstuffs that enter mitochondria to react with oxygen?
What are the foodstuffs that enter mitochondria to react with oxygen?
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How do enzymes influence the reactions inside mitochondria?
How do enzymes influence the reactions inside mitochondria?
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What role does the lipid bilayer play in cellular transport?
What role does the lipid bilayer play in cellular transport?
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What distinguishes simple diffusion from facilitated diffusion?
What distinguishes simple diffusion from facilitated diffusion?
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Describe the process and requirement of active transport.
Describe the process and requirement of active transport.
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What is aqueous diffusion and how does it occur in capillaries?
What is aqueous diffusion and how does it occur in capillaries?
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What types of substances typically undergo lipid diffusion, and why?
What types of substances typically undergo lipid diffusion, and why?
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How does the structure of the membrane influence diffusion?
How does the structure of the membrane influence diffusion?
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Why is energy required for active transport but not for diffusion?
Why is energy required for active transport but not for diffusion?
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What passive mechanisms govern the process of aqueous diffusion?
What passive mechanisms govern the process of aqueous diffusion?
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Study Notes
Functional Systems of the Cell: Transport
- Cells require transport of substances across the cell membrane to maintain homeostasis and function.
- The lipid bilayer acts as a barrier, preventing the free movement of water and water-soluble substances between extracellular and intracellular fluids.
- Membrane transport occurs through passive and active mechanisms.
Passive Transport
- Diffusion: Random movement of molecules, either through membrane spaces or with carrier proteins. It's driven by the kinetic energy of molecules moving from high to low concentration.
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Types of Diffusion:
- Simple diffusion: Movement through intermolecular spaces or directly across the lipid bilayer for lipid-soluble molecules.
- Facilitated diffusion: Movement assisted by carrier proteins, essential for larger or insoluble molecules, still from high to low concentration gradient.
- Aqueous diffusion: Movement of molecules through the watery extracellular and intracellular spaces, common in capillaries. This is a passive process following Fick's Law.
- Lipid diffusion: Movement of molecules through membranes, driven by lipid solubility.
- Fick's law: Predicts the rate of movement of molecules across a barrier, influenced by concentration gradient, permeability coefficient, area, and thickness of the barrier.
Active Transport
- Active transport: Movement of ions or other substances against an energy gradient (often from low to high concentration) using carrier proteins and an energy source beyond kinetic energy. Requires ATP.
- Carrier-mediated diffusion: Substances move with a carrier protein, but does not move against the concentration gradient, utilizing no energy.
Transport by Special Carriers
- Drugs can be transported across barriers by specialized carrier mechanisms (e.g., amino acid carriers in the blood-brain barrier, weak acid carriers in the renal tubules).
- Carrier transport isn't governed by Fick's law and is capacity-limited.
- Selective inhibitors of these carriers (like probenecid) can be useful clinically (e.g., increasing uric acid excretion).
Endocytosis
- Cells take in large particles via endocytosis.
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Types of Endocytosis:
- Pinocytosis: Ingestion of minute particles, creating vesicles of extracellular fluid.
- Phagocytosis: Ingestion of large particles (bacteria, cells).
- Mechanism: Membrane engulfs particles (often via receptor binding), forming vesicles, transporting the molecules inside the cell.
- Very large or insoluble molecules need this mechanism.
- Opsonization: Bacteria attach to antibodies to assist in cell engulfment.
Phagocytosis Steps
- Cell membrane receptors attach to ligands on a particle.
- Membrane engulfs the particle.
- Cytoplasmic actin and other contractile fibrils surround the vesicle, pushing it inwards until the particle is fully inside.
- Contractile proteins pinch off the vesicle, creating another intracellular vesicle inside the cell.
Lysosomes
- Almost immediately after endocytosis, lysosomes fuse with the vesicle, releasing digestive enzymes within the particles.
- Digestive vesicle is formed with enzymes hydrolyzing proteins, carbohydrates, lipids, etc., producing basic molecules like amino acids, glucose,etc. that diffuse to the cytoplasm.
- Lysosomes contain bactericidal agents (lysozyme, lysoferrin, acid) to destroy engulfed bacteria, or other foreign matter, before causing cellular damage.
- Indigestible remains become residual bodies and are eliminated from the cell by exocytosis.
Mitochondria
- Mitochondria are organelles responsible for cellular respiration and energy production.
- Foodstuffs are oxidized with oxygen inside mitochondria, releasing energy used to make ATP (adenosine triphosphate), which is the main energy currency for cells.
- ATP is used for various cellular processes (active transport, synthesis, and mechanical work (muscle contraction)).
General Cell Concepts
- Cellular transport mechanisms are essential for maintaining homeostasis and normal cellular operation.
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Description
Test your knowledge on various cell transport mechanisms, including diffusion, active transport, pinocytosis, and phagocytosis. Delve into how substances like vitamin B12 and iron enter cells, and understand the roles of protein structures and macromolecules in these processes. Explore the differences between active and passive transport, and the significance of selective inhibitors.