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Which of the following is NOT a recommended aspect for the title of a BIOL 110 lab report?
Which of the following is NOT a recommended aspect for the title of a BIOL 110 lab report?
In the context of a BIOL 110 lab report, what is the primary purpose of the 'Materials and Methods' section?
In the context of a BIOL 110 lab report, what is the primary purpose of the 'Materials and Methods' section?
According to the provided content, which section of the lab report is considered the main section?
According to the provided content, which section of the lab report is considered the main section?
What is the minimum number of figures required in the 'Results' section of a BIOL 110 lab report?
What is the minimum number of figures required in the 'Results' section of a BIOL 110 lab report?
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Which of the following is NOT a characteristic of a well-written 'Results' section in a BIOL 110 lab report?
Which of the following is NOT a characteristic of a well-written 'Results' section in a BIOL 110 lab report?
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Which of the following statements accurately reflects the expectation for the 'Abstract' section of the BIOL 110 lab report?
Which of the following statements accurately reflects the expectation for the 'Abstract' section of the BIOL 110 lab report?
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What is the primary function of the 'Introduction' section in a BIOL 110 lab report?
What is the primary function of the 'Introduction' section in a BIOL 110 lab report?
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According to the content, what is one key element of the 'Introduction' section that should be discussed?
According to the content, what is one key element of the 'Introduction' section that should be discussed?
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Which of the following statements about the use of microscopes in studying cells is FALSE?
Which of the following statements about the use of microscopes in studying cells is FALSE?
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Why are microscopes crucial to studying the behavior of cells?
Why are microscopes crucial to studying the behavior of cells?
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What is the fundamental difference between light microscopes (LMs) and electron microscopes (EMs) in visualizing cells?
What is the fundamental difference between light microscopes (LMs) and electron microscopes (EMs) in visualizing cells?
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Which of the following statements accurately describes the relationship between cell size and the need for microscopes?
Which of the following statements accurately describes the relationship between cell size and the need for microscopes?
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Which aspect of cell biology is NOT directly facilitated by the use of microscopes?
Which aspect of cell biology is NOT directly facilitated by the use of microscopes?
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What is the primary purpose of cell fractionation?
What is the primary purpose of cell fractionation?
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Which of the following processes is involved in the initial step of cell fractionation?
Which of the following processes is involved in the initial step of cell fractionation?
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During differential centrifugation, which pellet would be obtained from a centrifugation step at 80,000 g for 60 minutes?
During differential centrifugation, which pellet would be obtained from a centrifugation step at 80,000 g for 60 minutes?
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What does the supernatant contain after the initial centrifugation step at 1,000 g for 10 minutes?
What does the supernatant contain after the initial centrifugation step at 1,000 g for 10 minutes?
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Which characteristic of centrifugation is primarily utilized in cell fractionation?
Which characteristic of centrifugation is primarily utilized in cell fractionation?
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What is the primary function of contractile vacuoles in freshwater protists?
What is the primary function of contractile vacuoles in freshwater protists?
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Which of the following is NOT a feature shared between mitochondria and chloroplasts?
Which of the following is NOT a feature shared between mitochondria and chloroplasts?
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What is the primary function of the cristae in mitochondria?
What is the primary function of the cristae in mitochondria?
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Where does the Calvin cycle take place in chloroplasts?
Where does the Calvin cycle take place in chloroplasts?
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How do the thylakoids contribute to photosynthesis?
How do the thylakoids contribute to photosynthesis?
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What is the main evidence supporting the endosymbiotic theory?
What is the main evidence supporting the endosymbiotic theory?
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Which of the following statements accurately describes the relationship between the engulfed cell and the host cell in the endosymbiotic theory?
Which of the following statements accurately describes the relationship between the engulfed cell and the host cell in the endosymbiotic theory?
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What is the role of the inner membrane in both mitochondria and chloroplasts?
What is the role of the inner membrane in both mitochondria and chloroplasts?
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Which type of cell is characterized by the presence of a cell wall?
Which type of cell is characterized by the presence of a cell wall?
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Which of the following correctly describes the relationship between the nucleus and endoplasmic reticulum (ER)?
Which of the following correctly describes the relationship between the nucleus and endoplasmic reticulum (ER)?
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How do the ribosomes in mitochondria and chloroplasts differ from those found in the cytoplasm of eukaryotic cells?
How do the ribosomes in mitochondria and chloroplasts differ from those found in the cytoplasm of eukaryotic cells?
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What is the main difference between the structure of prokaryotic and eukaryotic cells?
What is the main difference between the structure of prokaryotic and eukaryotic cells?
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Which of the following is NOT a true statement about prokaryotic cells?
Which of the following is NOT a true statement about prokaryotic cells?
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What is the primary function of the Golgi apparatus in eukaryotic cells?
What is the primary function of the Golgi apparatus in eukaryotic cells?
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What is the main difference between phagocytosis and autophagy?
What is the main difference between phagocytosis and autophagy?
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Which of the following is NOT a true statement about cellular respiration?
Which of the following is NOT a true statement about cellular respiration?
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What is the primary limitation of standard light microscopy?
What is the primary limitation of standard light microscopy?
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Which microscope type is best for examining the external structure of specimens?
Which microscope type is best for examining the external structure of specimens?
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What structural detail can a transmission electron microscope (TEM) reveal?
What structural detail can a transmission electron microscope (TEM) reveal?
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Which of the following sizes corresponds to typical bacteria observed under a microscope?
Which of the following sizes corresponds to typical bacteria observed under a microscope?
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How does the resolution of an electron microscope compare to that of a light microscope?
How does the resolution of an electron microscope compare to that of a light microscope?
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Which component can be best visualized using electron microscopy?
Which component can be best visualized using electron microscopy?
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In what context are SEMs and TEMs utilized in cell biology?
In what context are SEMs and TEMs utilized in cell biology?
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What smallest size can be effectively resolved using electron microscopy?
What smallest size can be effectively resolved using electron microscopy?
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What is the most likely reason why insulin, a protein produced by the pancreas, is absent in a bacterial cell, even though both cells possess DNA that codes for proteins?
What is the most likely reason why insulin, a protein produced by the pancreas, is absent in a bacterial cell, even though both cells possess DNA that codes for proteins?
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While insulin mRNA is found in the cytoplasm and not the nucleus, where would you expect to find the corresponding insulin-encoding DNA in a pancreatic cell?
While insulin mRNA is found in the cytoplasm and not the nucleus, where would you expect to find the corresponding insulin-encoding DNA in a pancreatic cell?
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Based on the information provided, after being synthesized on the ribosomes, which organelle would insulin most likely travel to next in order to be modified and packaged for secretion?
Based on the information provided, after being synthesized on the ribosomes, which organelle would insulin most likely travel to next in order to be modified and packaged for secretion?
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Comparing the information presented about eukaryotic and prokaryotic cells, what is the most significant difference that would make the insulin-encoding DNA inaccessible in a bacterial cell?
Comparing the information presented about eukaryotic and prokaryotic cells, what is the most significant difference that would make the insulin-encoding DNA inaccessible in a bacterial cell?
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Imagine a hypothetical scenario where the Golgi apparatus in a pancreatic cell becomes dysfunctional. What would be the most immediate consequence for insulin production and secretion?
Imagine a hypothetical scenario where the Golgi apparatus in a pancreatic cell becomes dysfunctional. What would be the most immediate consequence for insulin production and secretion?
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Study Notes
Cell Structure and Function
- Biologists use microscopes and biochemistry tools to study cells.
- Cells are too small to be seen with the naked eye.
- Microscopes magnify images using glass lenses to focus light or electrons through an object.
- Light microscopes (LM) create magnified images using a series of glass lenses to focus light through an object.
- Electron microscopes (EM) use a beam of electrons to visualize subcellular structures.
- Scanning electron microscopes (SEMs) are used to study the outside surface of structures.
- Transmission electron microscopes (TEMs) are used to study the internal structure of cells.
Cell Fractionation
- Fractionates cells to separate major organelles.
- Centrifuges fractionate cells into their component parts based on density.
- The process starts with homogenizing the cells, which is the process of breaking apart the cells.
- The material is split into two fractions; a supernatant (liquid) and pellet (solid).
- Various amounts of force are used to separate the material into the different organelles according to their densities.
Eukaryotic Cells
- Eukaryotic cells have internal membranes that compartmentalize their functions.
- The fundamental unit of all organisms is a cell, either prokaryotic or eukaryotic.
- Prokaryotic cells are only found in the domains Bacteria and Archaea.
- Eukaryotic cells are found in protists, fungi, animals, and plants.
Nucleus
- The nucleus is the control center of the cell.
- It contains most of the DNA.
- The nucleus (organelle) is membrane-bound, keeping it separate from the cytoplasm.
- The nuclear envelope encloses the nucleus, controlling what enters or exits.
- It's a double membrane, each with its own lipid bilayer.
- Ribosomes in the nucleolus produce ribosomal RNA (rRNA).
- Chromatin is condensed DNA and proteins.
- Chromosomes are distinct DNA structures, a condensed form of chromatin.
Ribosomes
- Ribosomes are made of ribosomal RNA (rRNA) and protein.
- They synthesize proteins.
- Ribosomes can be free within the cytoplasm of the cell (free ribosome), attached to the endoplasmic reticulum or the nuclear envelope (bound ribosome).
Endomembrane System
- The endomembrane system regulates protein traffic
- The endomembrane system consists of:
- Nuclear envelope
- Endoplasmic reticulum (ER)
- Golgi apparatus
- Lysosomes
- Vacuoles
- Plasma membrane
- These components are either continuous or connected via transfer by vesicles.
Endoplasmic Reticulum (ER)
- The ER is composed of two distinct regions:
- Smooth ER which lacks ribosomes
- Rough ER which is studded with ribosomes.
- The ER plays a critical role in the biosynthesis of proteins, lipids, and carbohydrates.
Golgi Apparatus
- The Golgi apparatus modifies, sorts, and packages materials into transport vesicles.
Lysosomes
- Contains hydrolytic enzymes that digest macromolecules.
- Lysosomes recycle the cell's own organelles and macromolecules through autophagy.
Peroxisomes
- Specialized metabolic compartments, bounded by a single membrane.
- Involved in detoxification of oxidative stress.
Vacuoles
- Vesicles derived from the ER and Golgi.
- Contractile vacuoles pump excess water out of cells.
- Central vacuoles hold organic compounds in many mature plant cells.
Mitochondria
- Sites of cellular respiration.
- The inner membrane creates two compartments: an intermembrane space and mitochondrial matrix.
- Cristae present a large surface area for enzymes that synthesize ATP.
Chloroplasts
- Contain the green pigment chlorophyll for photosynthesis
- Chloroplasts are found in leaves and other green organs of plants and in algae.
Evolutionary Origins of Mitochondria and Chloroplasts
- Mitochondria and chloroplasts have similarities with bacteria.
- Enveloped by a double membrane.
- Contain free ribosomes and circular DNA molecules
- Grow and reproduce somewhat independently in cells.
- These similarities suggests an endosymbiotic origin.
Prokaryotes vs. Eukaryotes
- Prokaryotic cells lack a nucleus and membrane-bound organelles.
- Eukaryotic cells have a nucleus and membrane-bound organelles.
- Other distinctions are in the size, arrangement of DNA, presence of certain organelles and cell division process.
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Description
Test your knowledge on the structure and function of cells, including microscopy techniques and cell fractionation processes. This quiz covers various methods used to visualize cells and their components, essential for understanding biological systems.