Cell Organelles and Their Functions

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Questions and Answers

What is the primary use of a centrifuge in cell fractionation?

  • To measure the pH levels of cell organelles
  • To separate cell structures based on their relative density (correct)
  • To increase the temperature of the cell components
  • To enhance the visibility of proteins under a microscope

Which organelle is responsible for the synthesis of proteins?

  • Chloroplast
  • Golgi apparatus
  • Mitochondria
  • Ribosomes (correct)

Why are membranous organelles important in a cell?

  • They store cellular waste until disposal
  • They enhance the flow of cytoplasm
  • They allow for optimum pH and specific functions detached from the cytoplasm (correct)
  • They facilitate the movement of nutrients in and out of the cell

Which of the following is a double membrane-bound organelle?

<p>Chloroplast (D)</p> Signup and view all the answers

What role do radioactive labelled chemical substances play in cellular studies?

<p>They allow tracing of the pathway taken by secreted proteins. (D)</p> Signup and view all the answers

Which of the following is NOT a non-membranous organelle?

<p>Golgi apparatus (B)</p> Signup and view all the answers

Which type of endoplasmic reticulum is covered with ribosomes?

<p>Rough ER (D)</p> Signup and view all the answers

What is a function of lysosomes?

<p>They contain hydrolytic enzymes for digestion. (B)</p> Signup and view all the answers

What process occurs in the Golgi apparatus to modify proteins?

<p>Glycosylation (B)</p> Signup and view all the answers

Which of the following correctly describes the primary function of lysosomes?

<p>Digest engulfed bacteria and worn-out organelles (B)</p> Signup and view all the answers

What best describes the structure of mitochondria?

<p>Double membrane with rod shape (B)</p> Signup and view all the answers

What is the primary function of the rough endoplasmic reticulum (RER)?

<p>Modification and transport of proteins (D)</p> Signup and view all the answers

What is the role of the cristae in mitochondria?

<p>Increase surface area for ATP synthesis (A)</p> Signup and view all the answers

Lysosomes bud off from which organelle?

<p>Golgi apparatus (D)</p> Signup and view all the answers

What key role do ribosomes play in the cell?

<p>Site of translation and protein synthesis (B)</p> Signup and view all the answers

Which type of ribosome is found in prokaryotes?

<p>70S ribosomes (C)</p> Signup and view all the answers

What adaptations allow mitochondria to meet energy demands?

<p>Variable number according to cellular activity (C)</p> Signup and view all the answers

What is the primary composition of the interior solution of mitochondria known as the matrix?

<p>Circular DNA and ribosomes (A)</p> Signup and view all the answers

Which organelle is responsible for the synthesis of lipids, such as cholesterol?

<p>Smooth endoplasmic reticulum (A)</p> Signup and view all the answers

What type of enzymes do lysosomes contain?

<p>Hydrolytic enzymes such as proteases (B)</p> Signup and view all the answers

How does the Golgi apparatus function in the cell?

<p>It modifies and packages proteins for secretion (C)</p> Signup and view all the answers

What structural characteristic distinguishes the smooth endoplasmic reticulum from the rough endoplasmic reticulum?

<p>Tubular structure with no ribosomes (C)</p> Signup and view all the answers

What is the primary function of thylakoids in chloroplasts?

<p>Site of light-dependent reactions (D)</p> Signup and view all the answers

What is the role of shuttle vesicles in relation to the endoplasmic reticulum?

<p>They bud off from the ER and transport proteins to the Golgi apparatus (A)</p> Signup and view all the answers

Which component of chloroplasts connects the stacks of thylakoids?

<p>Inter granal lamellae (A)</p> Signup and view all the answers

What is a unique feature of both chloroplasts and mitochondria?

<p>Double membrane structure (D)</p> Signup and view all the answers

What do we call the end of the Golgi apparatus where vesicles bud off to transport materials?

<p>Maturing face (D)</p> Signup and view all the answers

Which statement accurately describes the stroma of chloroplasts?

<p>Fluid containing DNA and enzymes (A)</p> Signup and view all the answers

Which of the following statements about chloroplasts is true?

<p>Involved in photosynthesis (D)</p> Signup and view all the answers

What distinguishes plant cells from animal cells concerning organelles?

<p>Presence of chloroplasts (A)</p> Signup and view all the answers

Which statement about chloroplast structure is correct?

<p>Includes an outer membrane and inner folded membrane (D)</p> Signup and view all the answers

What role do ribosomes in chloroplasts play?

<p>Assist in protein synthesis (B)</p> Signup and view all the answers

Which of the following statements about mitochondria is true?

<p>Mitochondria contain circular DNA and replicate through binary fission. (C)</p> Signup and view all the answers

What role do centrioles play during nuclear division?

<p>They act as microtubule organizing centers. (B)</p> Signup and view all the answers

Which of the following functions is associated with the nucleus?

<p>Control of cell activities via gene expression. (B)</p> Signup and view all the answers

What structural feature distinguishes centrioles from other organelles?

<p>They are made of 9 groups of triplet microtubules. (B)</p> Signup and view all the answers

How do the membranes of the nuclear envelope contribute to nuclear function?

<p>They control material exchange with the cytoplasm. (C)</p> Signup and view all the answers

What is the primary function of mitochondria in cells?

<p>Production of ATP through aerobic respiration. (C)</p> Signup and view all the answers

Which of the following is an example of a function that mitochondria contribute to?

<p>Exocytosis of hormones. (D)</p> Signup and view all the answers

Which of the following features is true regarding centrioles?

<p>They align at poles during prophase of mitosis. (C)</p> Signup and view all the answers

Flashcards

Cell Fractionation

Separating cell structures based on their density using a centrifuge.

Radioactive Labeling

A method to track the movement of molecules within a cell by using radioactive isotopes.

Membranous Organelles

Organelles enclosed by membranes, allowing for specific functions and optimal conditions.

Non-Membranous Organelles

Organelles lacking a membrane, directly interacting with the cytoplasm.

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Endoplasmic Reticulum (ER)

A network of interconnected membranes forming flattened sacs or tubules.

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Rough ER

ER studded with ribosomes, involved in protein synthesis and modification.

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Nucleus

A double-membrane bound organelle that contains genetic material (DNA) and controls cell activity.

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Mitochondria

A double-membrane bound organelle responsible for cellular respiration, generating energy (ATP).

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Rough Endoplasmic Reticulum (RER)

A network of interconnected membranous sacs (cisternae) studded with ribosomes, involved in protein synthesis, modification and transport.

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Smooth Endoplasmic Reticulum (SER)

A network of interconnected membranous tubules lacking ribosomes, involved in lipid synthesis, detoxification and calcium storage.

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Ribosomes

These are small, non-membranous organelles composed of two subunits (large and small) responsible for protein synthesis.

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RER-Bound Ribosomes

Ribosomes attached to the RER synthesize proteins that are destined to be transported outside the cell or incorporated into membranes.

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Free Ribosomes

Ribosomes free in the cytoplasm synthesize proteins that are used inside the cell for intracellular functions.

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Golgi Apparatus

A stack of flattened, membrane-enclosed sacs (cisternae) involved in modifying, sorting, and packaging proteins and lipids.

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Shuttle Vesicle

A small sac that buds off from the ER and transports proteins to the Golgi apparatus.

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Golgi Vesicle

A small sac that buds off from the Golgi apparatus and delivers proteins to the cell membrane for secretion.

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What is the Golgi apparatus?

A network of flattened sacs (cisternae) and associated vesicles involved in packaging, modifying, and transporting proteins and lipids.

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What is glycosylation?

Glycosylation is the process of adding carbohydrates to proteins, creating glycoproteins. The Golgi apparatus is responsible for this process.

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What is the function of Golgi vesicles?

The Golgi apparatus produces vesicles that carry synthesized proteins and lipids to other parts of the cell or outside the cell.

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What are lysosomes?

Lysosomes are membrane-bound organelles that contain hydrolytic enzymes. These enzymes are responsible for breaking down waste materials, worn-out organelles, and engulfed viruses.

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What are primary lysosomes?

Primary lysosomes are formed from the Golgi apparatus and contain hydrolytic enzymes synthesized on the ER.

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What are mitochondria?

Mitochondria are double-membrane bound organelles responsible for producing ATP through aerobic respiration.

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What is the function of the cristae in mitochondria?

The inner membrane of mitochondria forms folds called cristae, which increase the surface area available for ATP production.

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What is contained within the matrix of mitochondria?

The matrix of mitochondria contains circular DNA and ribosomes, allowing mitochondria to synthesize their own proteins and replicate independently.

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What is the endosymbiont theory?

Mitochondria are double-membrane bound organelles with their own DNA and ribosomes. They are thought to have originated from free-living bacteria engulfed by eukaryotic cells.

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What are centrioles?

Centrioles are non-membranous organelles found in animal cells, but not plant cells. They are made of microtubules arranged in triplets and play a crucial role in cell division.

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What is the function of centrioles during cell division?

Centrioles are involved in the formation of spindle fibers during cell division, which helps separate chromosomes.

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What is the nucleus?

The nucleus is the largest organelle in a cell, enclosed by a double membrane called the nuclear envelope. It contains DNA and controls all cell activities, including cell division.

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What are nuclear pores?

The nuclear envelope has pores that regulate the movement of molecules between the nucleus and cytoplasm, ensuring proper communication and function.

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What does the nucleus contain?

The nucleus contains DNA, organized into chromatin, which carries genes responsible for controlling cell division, activity, and inheritance.

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How does the nucleus control cell division?

The nucleus is responsible for controlling cell division, as it contains genetic information that determines the characteristics of the offspring cells.

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What are ribosomes?

A small, non-membranous organelle that's involved in making proteins. They're made up of two subunits, a large one and a small one.

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What is mRNA?

A type of RNA (ribonucleic acid) that carries the genetic code from the nucleus to the ribosomes, where protein synthesis occurs.

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What are chloroplasts?

They are double-membrane bound organelles found in plants that contain chlorophyll and carry out photosynthesis, the process of converting light energy into chemical energy.

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What are Grana?

Stacks of disc-shaped sacs (thylakoids) in a chloroplast, where the light-dependent reactions of photosynthesis occur.

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What is the stroma?

The fluid inside a chloroplast that contains the chloroplast's own DNA, ribosomes, and enzymes. It's the site of the light-independent reactions of photosynthesis.

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What are thylakoids?

A flat, disc-shaped compartment within a chloroplast's grana. They contain chlorophyll and are the sites of the light-dependent reactions of photosynthesis.

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Study Notes

Cell Organelles and Their Functions

  • Cell fractionation uses centrifugation to separate cell structures based on their density. Higher centrifuge speeds result in greater separation ability.
  • Radioactive labeled chemicals are used to track specific cell components. Radioactively labeled amino acids can identify protein synthesis pathways.

Cell Organelle Structure and Function

  • Cell organelles are grouped into membrane-bound and non-membrane-bound organelles.
  • Membrane-bound organelles are separated from cytoplasm by a membrane, allowing for specific pH and function. Examples include mitochondria, chloroplasts, lysosomes, ER, Golgi apparatus, vacuoles, and secretory vesicles.
  • Single membrane bound organelles include large vacuoles, endoplasmic reticulum, Golgi apparatus, lysosomes, and secretory vesicles.
  • Double membrane bound organelles include nucleus, mitochondria, chloroplast, amyloplast.

Endoplasmic Reticulum

  • The endoplasmic reticulum is a membrane-bound organelle connected to the nuclear envelope.
  • RER has ribosomes bound to its surface, performing protein synthesis and protein folding.
  • Proteins are transported in vesicles from RER to Golgi apparatus.
  • SER does not have ribosomes, synthesizes lipids, carbohydrates (e.g., cholesterol, testosterone).

Ribosomes

  • Ribosomes are non-membrane-bound organelles.
  • They are made of rRNA and proteins.
  • Two types of ribosomes: 70S (prokaryotes, mitochondria, chloroplasts) and 80S (eukaryotes - cytoplasm).
  • Ribosomes synthesize proteins in the cytoplasm or on the RER.

Golgi Apparatus

  • Golgi apparatus modifies proteins from RER, packages them for secretion or transport to other cell parts.
  • Involved in processing and packaging lipids, and producing components of lysosomes.

Lysosomes

  • Lysosomes are single-membrane bound, spherical sacs.
  • These contain digestive enzymes (proteases, lipases) to break down excess or worn-out cell components, food particles, and engulfed viruses/bacteria.

Mitochondria

  • Mitochondria are double membrane-bound, rod-shaped organelles.
  • The inner membrane is folded into cristae, increasing surface area for ATP production during cellular respiration.
  • Mitochondria are responsible for aerobic respiration and lipid synthesis.

Nucleus

  • The nucleus is a double membrane-bound, spherical organelle.
  • The nuclear envelope regulates the passage of mRNA and proteins in and out.
  • Contains chromatin (DNA, proteins), and nucleolus (where ribosomes are synthesized).
  • Controls cell activities and carries inheritance information.

Chloroplast

  • Chloroplasts are double membrane bound organelles.
  • Found in plant cells, responsible for photosynthesis.
  • Consists of thylakoids that are stacked into grana, and stroma that surround the grana
  • Has own DNA and ribosomes (70S).

Centrioles

  • Centrioles are non-membrane-bound organelles.
  • Found in animal cells, involved in cell division by organizing microtubules during prophase.
  • Centrioles are part of a larger centrosome structure.

Notice:

  • The number of mitochondria in a cell depends on the cell's energy demand(eg., liver cells have many mitochondria for energy needs).
  • The endosymbiotic theory suggests that mitochondria and chloroplasts were once free-living prokaryotes.
  • Plant cells have no centrioles or lysosomes.

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