Cell Membrane Structure and Function

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Questions and Answers

Which characteristic of phospholipids is most responsible for their spontaneous assembly into bilayers?

  • The presence of glycerol backbone
  • The presence of phosphate groups
  • Their high molecular weight
  • Their amphiphilic nature (correct)

The two leaflets of the plasma membrane always maintain a symmetrical distribution of phospholipids to ensure uniform membrane properties.

False (B)

Briefly explain how the presence of cholesterol affects the fluidity of an animal cell membrane at different temperatures.

Cholesterol acts as a fluidity buffer, reducing fluidity at high temperatures and preventing rigidification at low temperatures.

In plant cells, the structural strength provided in addition to the plasma membrane is from a carbohydrate-based cell __________.

<p>wall</p> Signup and view all the answers

Match the following lipids with their primary function or characteristic within cell membranes:

<p>Phospholipids = Main structural component of cell membranes, forming a bilayer. Cholesterol = Modulates membrane fluidity and stability in animal cells. Sphingolipids = Participate in cell signaling and membrane organization. Phytosterols = Analogous to cholesterol in plant cells.</p> Signup and view all the answers

Which of the following factors contributes most significantly to the formation of lipid rafts within the plasma membrane?

<p>Specific lipid-lipid and lipid-protein interactions (C)</p> Signup and view all the answers

Saturated fatty acid tails in phospholipids increase membrane fluidity due to their inability to pack tightly together.

<p>False (B)</p> Signup and view all the answers

Explain how the asymmetric distribution of phospholipids in the plasma membrane leaflets contributes to cell signaling.

<p>Specific phospholipids, like phosphatidylserine, are normally located on the inner leaflet but can be flipped to the outer leaflet to signal apoptosis or other cellular events.</p> Signup and view all the answers

The presence of a cis double bond in the unsaturated fatty acid tail of a phospholipid introduces a __________ in the tail, enhancing membrane fluidity.

<p>kink</p> Signup and view all the answers

How do plant cells compensate for the lack of cholesterol in their plasma membranes to maintain membrane integrity?

<p>By utilizing phytosterols, which are structurally similar to cholesterol (C)</p> Signup and view all the answers

Which of the following is the MOST direct consequence of amphotericin B and nystatin binding to ergosterol in fungal cells?

<p>Formation of ion pores in the membrane, disrupting ion gradients. (B)</p> Signup and view all the answers

The endoplasmic reticulum (ER) membrane is initially asymmetric, and this asymmetry is directly inherited by the plasma membrane.

<p>False (B)</p> Signup and view all the answers

Briefly explain how the amphiphilic nature of phospholipids drives the self-assembly of lipid bilayers in aqueous environments.

<p>The polar head groups of phospholipids interact favorably with water, while the hydrophobic tails cluster together to avoid water, leading to the formation of a bilayer structure.</p> Signup and view all the answers

In the fluid mosaic model, the 'fluid' aspect refers to the ability of membrane components to move _________ within the plane of the membrane.

<p>laterally</p> Signup and view all the answers

Match each antifungal drug with its mechanism of action:

<p>Amphotericin B = Forms ion pores by binding to ergosterol Nystatin = Forms ion pores by binding to ergosterol Miconazole = Inhibits ergosterol synthesis Lamisil = Inhibits ergosterol synthesis</p> Signup and view all the answers

Which of the following lipid components is predominantly found in the outer leaflet of the plasma membrane?

<p>Sphingomyelin (A)</p> Signup and view all the answers

The presence of cholesterol in the plasma membrane always decreases membrane fluidity, regardless of temperature.

<p>False (B)</p> Signup and view all the answers

Describe a specific function of membrane proteins that contributes to cell communication or interaction with the external environment.

<p>Membrane proteins act as receptors for signaling molecules, allowing cells to respond to external stimuli and coordinate their activities.</p> Signup and view all the answers

The conversion of a symmetric ER membrane into an asymmetric plasma membrane involves the action of enzymes called _________ that selectively transfer specific phospholipids to different leaflets.

<p>flippases</p> Signup and view all the answers

Which of the following BEST explains why ergosterol is an effective target for antifungal drugs?

<p>Ergosterol is uniquely present in fungal cells and is critical for their membrane integrity. (D)</p> Signup and view all the answers

Which of the following characteristics distinguishes integral membrane proteins from peripheral membrane proteins?

<p>Integral proteins are permanently embedded within the lipid bilayer, whereas peripheral proteins are only temporarily associated with the membrane surface. (B)</p> Signup and view all the answers

What is the primary function of cellulose in the plant cell wall, and what property does it provide?

<p>Cellulose provides tensile strength to the plant cell wall.</p> Signup and view all the answers

The structural rigidity of the plant cell wall allows for the generation of a large internal pressure known as ______ pressure.

<p>turgor</p> Signup and view all the answers

Match the following components of the plant cell wall with their primary function:

<p>Cellulose = Provides tensile strength Pectin = Provides resistance to compression Lignin = Waterproofing</p> Signup and view all the answers

What would happen to a plant cell without a cell wall when placed in a hypotonic solution?

<p>It would swell and eventually burst. (C)</p> Signup and view all the answers

Which of the following is NOT a component of the plant cell wall?

<p>Chitin (A)</p> Signup and view all the answers

Animal cells possess a cell wall that provides structural support and prevents them from bursting in hypotonic environments.

<p>False (B)</p> Signup and view all the answers

Describe the role of turgor pressure in plant cell expansion, especially during growth.

<p>Turgor pressure drives cell expansion by exerting force on the cell wall, allowing the cell to increase in size.</p> Signup and view all the answers

Which lipid type is most abundant in plasma membranes?

<p>Phospholipids (D)</p> Signup and view all the answers

The presence of a cis double-bond in the hydrocarbon tail of a phospholipid decreases membrane fluidity.

<p>False (B)</p> Signup and view all the answers

What is the structural characteristic that defines a molecule as amphipathic?

<p>having both polar and non-polar regions</p> Signup and view all the answers

In animal cell membranes, the major sterol present is ________.

<p>cholesterol</p> Signup and view all the answers

What is the primary function of sterols, such as cholesterol, in the plasma membrane?

<p>To stiffen portions of the phospholipids, enhancing structural integrity (C)</p> Signup and view all the answers

How do plant cells differ from animal cells regarding membrane sterols?

<p>Plant cells use a combination of phytosterols. (B)</p> Signup and view all the answers

Saturated hydrocarbon tails in phospholipids generally increase membrane fluidity compared to unsaturated tails.

<p>False (B)</p> Signup and view all the answers

Which of the following molecules would you expect to diffuse freely through the plasma membrane?

<p>Steroid hormones (D)</p> Signup and view all the answers

Why is ergosterol a useful target for antifungal drugs?

<p>It is found in fungi but not in animal cells. (B)</p> Signup and view all the answers

Amphotericin B and nystatin inhibit ergosterol synthesis, blocking fungal cell growth.

<p>False (B)</p> Signup and view all the answers

Describe how the amphiphilic nature of phospholipids contributes to the self-assembly of lipid bilayer membranes.

<p>The polar head groups associate with water on the outside of the membrane, while hydrocarbon tails associate with each other on the inside, forming a bilayer structure.</p> Signup and view all the answers

According to the fluid mosaic model, the plasma membrane is considered a fluid because of the ability of its components to move ________ within the membrane.

<p>laterally</p> Signup and view all the answers

What is the origin of asymmetry in the plasma membrane?

<p>It is derived from the symmetric ER membrane and then modified. (D)</p> Signup and view all the answers

Match the membrane lipid with its location:

<p>Sphingomyelin = Outer leaflet Ganglioside = Outer leaflet Phosphatidylserine = Inner leaflet Phosphatidylethanolamine = Inner leaflet</p> Signup and view all the answers

Which of the following is NOT a key function provided by membrane proteins?

<p>Cell Wall Synthesis (C)</p> Signup and view all the answers

Describe how the asymmetric distribution of lipids in the plasma membrane contributes to cell function. Provide a specific example.

<p>Lipid asymmetry affects membrane curvature, protein function, and signaling. For example, phosphatidylserine on the inner leaflet has a negative charge, which recruits positively charged signaling molecules.</p> Signup and view all the answers

Which type of membrane protein is embedded within the lipid bilayer, potentially spanning both leaflets?

<p>Integral protein (A)</p> Signup and view all the answers

What is the main component of the plant cell wall that provides tensile strength?

<p>cellulose</p> Signup and view all the answers

In plant cells, turgor pressure is generated due to the influx of water via ______.

<p>osmosis</p> Signup and view all the answers

Match the following membrane structures with their descriptions:

<p>Liposomes = Spherical vesicles formed by phospholipids in water Black membranes = Planar lipid bilayers formed across a hole in a partition Cellulose microfibrils = Provides tensile strength to plant cell walls Pectin = Provides resistance to compression in plant cell walls</p> Signup and view all the answers

Animal cells rely on turgor pressure for structural rigidity, similar to plant cells.

<p>False (B)</p> Signup and view all the answers

Which component of the plant cell wall provides waterproofing and is often found in woody tissues?

<p>Lignin (C)</p> Signup and view all the answers

Explain how turgor pressure contributes to plant cell growth.

<p>Turgor pressure provides the driving force for cell expansion by exerting pressure against the cell wall, allowing the cell to stretch and grow.</p> Signup and view all the answers

The tensile strength of cellulose in the plant cell wall is comparable to ______.

<p>steel</p> Signup and view all the answers

Explain how the amphipathic nature of phospholipids contributes to the formation of the plasma membrane's bilayer structure.

<p>Phospholipids have a polar (hydrophilic) head and non-polar (hydrophobic) tails. In an aqueous environment, the hydrophobic tails aggregate to exclude water, forming a bilayer with the hydrophilic heads facing outwards towards the water.</p> Signup and view all the answers

Why do unsaturated fatty acids increase membrane fluidity?

<p>Unsaturated fatty acids have <em>cis</em> double bonds that introduce kinks in the hydrocarbon tails. These kinks prevent tight packing of the lipids, increasing the fluidity of the membrane.</p> Signup and view all the answers

How would a plasma membrane adapt to maintain its fluidity in colder temperatures?

<p>The membrane will incorporate more unsaturated fatty acids within the phospholipids. The <em>cis</em> double bonds introduce kinks, preventing the lipids from packing tightly together, thus maintaining fluidity.</p> Signup and view all the answers

What is the role of sterols, such as cholesterol, in the plasma membrane?

<p>Sterols like cholesterol insert themselves into the lipid bilayer and stiffen the membrane. They reduce the mobility of the phospholipids, making the membrane less deformable and decreasing its permeability to small water-soluble molecules.</p> Signup and view all the answers

Predict what would happen to a cell if its plasma membrane suddenly lost its selective permeability.

<p>If the plasma membrane lost its selective permeability, the cell would lose its ability to maintain distinct internal and external environments. Essential molecules could leak out, while harmful substances could freely enter, disrupting cellular functions and potentially leading to cell death.</p> Signup and view all the answers

What are lipid rafts and how do they contribute to the function of the plasma membrane?

<p>Lipid rafts are microdomains within the plasma membrane that are enriched in cholesterol and sphingolipids. They serve as platforms for organizing membrane proteins and lipids, influencing membrane fluidity, protein trafficking, and signal transduction.</p> Signup and view all the answers

Explain why the distribution of phospholipids is asymmetric in the plasma membrane.

<p>The asymmetric distribution of phospholipids in the plasma membrane is due to the specific functions carried out by different phospholipids in each leaflet (inner and outer layers). Some phospholipids play a greater role interacting with cytosolic proteins, while others are important for cell signaling or protection against the external environment.</p> Signup and view all the answers

Contrast the main function of the plasma membrane in animal cells versus the function of the plant cell wall in plant cells.

<p>The plasma membrane in animal cells primarily regulates the transport of substances in and out of the cell and facilitates cell signaling. The plant cell wall, on the other hand, primarily provides structural support and protection to the plant cell, maintaining its shape and withstanding turgor pressure.</p> Signup and view all the answers

Explain why the amphiphilic nature of phospholipids is crucial for the self-assembly of biological membranes, and what would happen if phospholipids were purely hydrophobic?

<p>The amphiphilic nature, having both polar and nonpolar regions, allows phospholipids to form bilayers in aqueous environments spontaneously, with the polar heads interacting with water and the hydrophobic tails clustering together. If phospholipids were purely hydrophobic, they would likely aggregate irregularly, without forming a bilayer structure.</p> Signup and view all the answers

How does the 'fluid mosaic' model describe the structure of the plasma membrane, and what components contribute to its fluidity and mosaic nature?

<p>The fluid mosaic model describes the plasma membrane as a fluid structure with a mosaic of various proteins embedded in a phospholipid bilayer. The fluidity is mainly due to the movement of lipids and proteins within the membrane, and the mosaic nature is due to the diverse proteins and other molecules scattered throughout the lipid matrix.</p> Signup and view all the answers

Describe how the asymmetry of the plasma membrane is established, starting from the symmetric ER membrane, and provide one functional implication of this asymmetry.

<p>The asymmetry of the plasma membrane is established through the action of flippases, floppases, and scramblases, which selectively transfer specific lipids to either the cytosolic or exoplasmic face of the membrane as it moves from the ER to the Golgi and finally the plasma membrane. One functional implication is in cell signaling, where certain lipids (e.g., phosphatidylserine) are exposed on the outer leaflet to signal apoptosis.</p> Signup and view all the answers

Based on the information provided, how do antifungal drugs like amphotericin B and miconazole target fungal cells, and why are these drugs relatively safe for animal cells?

<p>Amphotericin B and nystatin target fungal cells by binding to ergosterol and forming ion pores, leading to leakage of ions. Miconazole and lamisil inhibit ergosterol synthesis. They are relatively safe because animal cells do not contain ergosterol, so the drugs selectively affect fungal cells.</p> Signup and view all the answers

Outline the various functions that membrane proteins carry out within the plasma membrane, giving at least three distinct examples.

<p>Membrane proteins perform functions such as molecular transport (e.g., channels and pumps), signal transduction (e.g., receptors), and structural support (e.g., anchoring to the cytoskeleton).</p> Signup and view all the answers

Imagine a cell needs to increase its membrane fluidity in response to colder temperatures. Describe two specific changes it could make to the lipid composition of its plasma membrane to achieve this.

<p>To increase membrane fluidity, the cell could increase the proportion of unsaturated fatty acids in the phospholipids, which introduce kinks and prevent tight packing. It could also incorporate more cholesterol, which acts as a fluidity buffer; at low temperatures, it prevents the membrane from solidifying.</p> Signup and view all the answers

If a researcher introduces a protein that disrupts lipid rafts within a cell membrane, what potential effects might this have on cellular processes, and why?

<p>Disrupting lipid rafts could affect cell signaling, membrane trafficking, and protein sorting because these rafts concentrate specific proteins and lipids involved in these processes. Disrupting them disperses these components, reducing the efficiency of associated functions.</p> Signup and view all the answers

Explain how the structure of a transmembrane protein determines its integration into the lipid bilayer, and what types of amino acids would be most likely found within the region of the protein that spans the membrane?

<p>Transmembrane proteins have hydrophobic amino acid side chains in the region spanning the lipid bilayer, allowing them to interact favorably with the hydrophobic core. This hydrophobic region anchors the protein in the membrane, while hydrophilic regions are exposed to the aqueous environment.</p> Signup and view all the answers

Describe two distinct ways integral membrane proteins can interact with the lipid bilayer.

<p>Integral membrane proteins can either span both leaflets of the bilayer via a transmembrane region, or they can be anchored to one leaflet through the attachment of lipid groups.</p> Signup and view all the answers

Briefly describe the composition of the plant cell wall.

<p>The plant cell wall is composed primarily of cellulose, a glucose polymer providing tensile strength. It also includes pectin, a complex polysaccharide that provides resistance to compression, as well as other cross-linking polysaccharides and lignin, which provides waterproofing.</p> Signup and view all the answers

Explain how turgor pressure is generated in plant cells and why it is important.

<p>Turgor pressure is generated when water flows into the cell via osmosis due to a higher solute concentration inside. The cell wall resists swelling, creating internal pressure. This sustains cell rigidity preventing wilting and provides the driving force for cell expansion during growth.</p> Signup and view all the answers

How does the plant cell wall prevent a plant cell from bursting in a hypotonic environment?

<p>In a hypotonic environment, water enters the cell due to osmosis. The rigid cell wall resists the expansion of the cell, preventing it from swelling excessively and bursting. Instead, the cell builds up turgor pressure.</p> Signup and view all the answers

What role does cellulose play in the plant cell wall, and what property does it provide?

<p>Cellulose is the major component of the plant cell wall. It provides tensile strength, which is comparable to steel, allowing the cell wall to withstand pulling forces without breaking.</p> Signup and view all the answers

Explain how the arrangement of cellulose and pectin contributes to the overall strength and flexibility of the plant cell wall.

<p>Cellulose microfibrils provide tensile strength, resisting pulling forces. Pectin, interwoven with the cellulose, provides resistance to compression. Together, they create a matrix that is strong yet flexible, allowing the cell wall to withstand various stresses.</p> Signup and view all the answers

Explain why animal cells do not have turgor pressure like plant cells.

<p>Animal cells lack a cell wall, which is essential for withstanding the osmotic pressure caused by water influx. Without a cell wall, animal cells would swell and burst in a hypotonic environment, preventing the development of stable turgor pressure.</p> Signup and view all the answers

Flashcards

Plasma Membrane

The outer boundary of a cell, composed mainly of phospholipids and proteins.

Phospholipids

Lipids with a polar head and two non-polar tails, forming the main structure of cell membranes.

Lipid Bilayer Formation

The spontaneous assembly of phospholipids into a double-layered structure in water.

Membrane Fluidity

The ability of lipids and proteins to move laterally within the membrane.

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Lipid Rafts

Specialized regions within the plasma membrane with distinct lipid and protein compositions.

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Membrane Asymmetry

The different compositions of phospholipids between the inner and outer layers of the plasma membrane.

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Sterols

Molecules with rigid ring structures found in membranes, like cholesterol in animal cells.

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Amphipathic

Having both polar (water-attracting) and non-polar (water-repelling) parts

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Phosphoglycerides

Lipids based on glycerol, common in membranes.

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Sphingolipids

Lipids with a sphingosine backbone, found in cell membranes.

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Ergosterol

A sterol found in fungi, serving as a target for antifungal drugs.

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Amphotericin B & Nystatin

Antifungal drugs that bind to ergosterol and create ion pores in fungal membranes, causing leakage.

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Miconazole & Lamisil

Antifungal drugs that inhibit ergosterol synthesis, thus stopping fungal cell growth.

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Self-Assembly of Phospholipid Membranes

The spontaneous assembly of phospholipids into a bilayer structure in aqueous solution.

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Fluid Mosaic Model

Describes the plasma membrane as a dynamic structure where proteins and lipids move freely.

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ER membrane (symmetric)

The plasma membrane derives its symmetry from this organelle membrane.

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Functions of Membrane Proteins

Transport molecules, signal transduction, anchorage and cell interaction.

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Membrane Proteins

The plasma membrane contains these, as well as lipids.

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Molecular transport (Membrane Proteins)

An important function is molecular transport across the membrane.

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Peripheral Membrane Proteins

Proteins bound to the membrane surface.

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Integral Membrane Proteins

Proteins inserted into the membrane interior.

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Liposomes

Spherical vesicles of phospholipids that assemble spontaneously in water.

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Black Membranes

Planar lipid bilayers formed across a hole between two aqueous compartments.

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Plant Cell Wall

Carbohydrate matrix surrounding plant cells, outside the plasma membrane.

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Cellulose

A glucose polymer that provides tensile strength to the plant cell wall.

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Pectin

Mixture of polysaccharides in the plant cell wall that provides resistance to compression.

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Turgor Pressure

Internal pressure in plant cells due to water influx.

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Osmosis in Plant Cells

Water moves into the cell because of high solute concentration.

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Transmembrane proteins

Proteins with hydrophobic domains that pass through the lipid bilayer.

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Cholesterol

Lipid component of animal cell membranes that stiffens the membrane.

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Lateral Diffusion

Lateral movement of lipids and proteins within the membrane.

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Asymmetric Distribution

Non-symmetrical distribution of different phospholipids.

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Phospholipid Self-Assembly

Spontaneous formation of a bilayer due to hydrophobic/philic properties.

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Membrane Orientation

Heads point to water; tails hide inside.

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Membrane Protein Functions

Proteins perform transport, signaling, and anchoring functions.

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Transmembrane Region

A region of a protein that spans the cell membrane.

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Membrane Lipids

The most abundant lipids in plasma membranes.

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Unsaturated Tail

Increases membrane fluidity due to the kink in tails.

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Steroid Hormones

Diffuse freely through the plasma membrane.

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Phosphatidylcholine

Common phospholipid found mainly on the outer leaflet of the plasma membrane.

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Phosphatidylserine

A phospholipid typically found on the inner leaflet of the plasma membrane.

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Sphingomyelin

A common sphingolipid found in animal cell membranes.

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Ganglioside

Glycolipid with a sialic acid residue, found on the outer leaflet of the plasma membrane.

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Study Notes

  • Cells are bounded by the plasma membrane, composed mainly of phospholipids with sterols like cholesterol in animal cells.
  • Phospholipids spontaneously form bilayers, the fundamental structure of cellular membranes.
  • Lipid bilayers are 2D fluids allowing lateral diffusion of components but contain microdomains known as lipid rafts with varying compositions.
  • The two leaflets of the plasma membrane have an asymmetric distribution of phospholipids.
  • The plasma membrane contains many proteins that perform critical functions.
  • Plant cells have a carbohydrate based cell wall for additional structural support.

Phospholipids Composition

  • Phospholipids are the most abundant lipids found in plasma membranes.
  • Phospholipids are amphiphilic (amphipathic), possessing a polar head group and two non-polar hydrocarbon tails.
  • One tail is generally saturated, lacking double bonds, while the other is unsaturated with one cis double bond which increases membrane fluidity.

Sterols and Membrane Function

  • Sterols are another important lipid component of membranes.
  • Rigid ring structures within sterols stiffen phospholipids, which is essential for structural integrity.
  • Cholesterol is the major sterol in animal cell membranes.
  • Phytosterols are used by plant cells, while ergosterol is used by fungi.

Ergosterol and Antifungal Drugs

  • Ergosterol is a useful target for antifungal drugs to block fungal growth since it is found in fungi but not animal cells.
  • Amphotericin B and nystatin bind specifically to ergosterol and create ion pores, causing ions to leak out of the cell.
  • Miconazole and lamisil inhibit ergosterol synthesis.

Self-Assembly of Phospholipid Membranes

  • Due to their amphiphilic nature, phospholipids self-assemble into lipid bilayer membranes.
  • Polar head groups interact with water on the outer surfaces of the membrane.
  • Hydrocarbon tails interact with each other in the membrane's interior.

Studying Lipid Membrane Properties

  • Researchers can investigate the properties of artificial lipid bilayers in the lab.
  • Liposomes are spherical vesicles that are self assembled from phospholipids in water.
  • Liposome size varies with conditions like lipid concentration and specific phospholipids.
  • Black membranes are planar lipid bilayers formed across a hole separating two aqueous compartments.
  • Black membranes can be used to examine motion of individual lipid molecules, or other molecules incorporated into the membranes.

Plant Cell Wall Composition

  • Every plant cell is surrounded by a carbohydrate matrix referred to as the plant cell wall.
  • The plant cell wall is separate from, and outside of the plasma membrane of the plant cell
  • Cellulose, a glucose polymer, is the main component that provides tensile strength comparable to steel.
  • Cellulose microfibrils are interwoven with pectin (a complex mixture of polysaccharides) that provides resistance to compression.
  • Other components include additional cross-linking polysaccharides and lignin (waterproofing).

Plant Cell Turgor

  • The structural rigidity of the plant cell wall allows the generation of a large internal pressure known as turgor pressure.
  • Water will flow into the cell via osmosis when the intracellular environment has an excess of solutes.
  • Without a cell wall, the cell would swell until equilibrium is reached, or until the cell burst
  • The cell wall provides resistance to swelling, even under hydrostatic pressure of 10 or more atmospheres.
  • This provides rigidity to the cells and is the reason plants wilt when dehydrated. It is also the driving force for cell expansion during growth.

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