Cell Division: Meiosis vs. Mitosis

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Questions and Answers

How many nuclear divisions are required in meiosis?

  • One
  • Three
  • Two (correct)
  • Four

Which statement is true regarding the chromosome behavior during mitosis?

  • Produces daughter cells genetically different.
  • Centromeres survive through anaphase.
  • Centromeres dissolve in mitotic anaphase. (correct)
  • Chromosomes synapse and cross over.

What is the primary function of meiosis?

  • Cloning
  • Sexual reproduction (correct)
  • Asexual reproduction
  • Growth and repair

Which of the following accurately describes the products of meiosis?

<p>Four haploid cells that are non-identical (B)</p> Signup and view all the answers

What occurs at the G1 checkpoint of the cell cycle?

<p>Apoptosis is initiated if DNA is damaged. (D)</p> Signup and view all the answers

Which type of cells can stem cells produce?

<p>Various types of blood cells (A)</p> Signup and view all the answers

In which phase of the cell cycle does DNA replication occur?

<p>S phase (C)</p> Signup and view all the answers

What is the outcome if chromosomes are not properly aligned during mitosis?

<p>Mitosis will not continue. (D)</p> Signup and view all the answers

Which of the following statements about centromeres is true?

<p>They survive anaphase I in meiosis. (D)</p> Signup and view all the answers

What is one purpose of therapeutic cloning?

<p>To create therapeutic human tissues. (B)</p> Signup and view all the answers

What is the primary function of mitosis during the M stage of the cell cycle?

<p>Nuclear division (B)</p> Signup and view all the answers

In incomplete dominance, what is the expected phenotype of a heterozygote?

<p>A blend of the two homozygote phenotypes (D)</p> Signup and view all the answers

How many versions of a gene exist for a trait in a population where multiple alleles are present?

<p>At least three versions (D)</p> Signup and view all the answers

In a dihybrid cross, which of the following is essential?

<p>Crossing plants with different traits (D)</p> Signup and view all the answers

What term describes the phenotype resulting from codominance?

<p>The full expression of both alleles equally (B)</p> Signup and view all the answers

What best describes the genetic makeup of a homozygous individual?

<p>Identical alleles for a specific trait (A)</p> Signup and view all the answers

Which of the following statements is true regarding sex linkage?

<p>Traits influenced by sex chromosomes can be dominant or recessive (A)</p> Signup and view all the answers

What aspect is crucial when writing the methods section of a scientific lab report?

<p>Providing repeatable and detailed procedures (B)</p> Signup and view all the answers

Which of the following describes the outcome of cytokinesis?

<p>Division of cytoplasm (B)</p> Signup and view all the answers

Which statement best reflects the principles of a monohybrid cross?

<p>It focuses on the inheritance of a single trait (D)</p> Signup and view all the answers

Which of the following best describes the mechanism of action for β-lactam antibiotics?

<p>They covalently bond to Penicillin Binding Proteins. (A)</p> Signup and view all the answers

What is the primary role of β-lactamases in bacterial resistance?

<p>To deactivate β-lactam antibiotics. (B)</p> Signup and view all the answers

When extracting soil samples, what must one ensure before scooping up the sample?

<p>The container is sterile and closed. (A)</p> Signup and view all the answers

What sequence of actions is correct for using a micropipette?

<p>Adjust dial, place a clean tip, press plunger down, then release. (A)</p> Signup and view all the answers

Which of the following components is NOT required to be labeled on an agar plate?

<p>The type of bacteria tested (C)</p> Signup and view all the answers

Which method is used to create a 'soil smoothie' for bacterial extraction?

<p>Stirring soil with distilled water to create a suspension. (D)</p> Signup and view all the answers

What is the significance of the BLA, BLA-TEM, and Bla SHV genes in soil samples?

<p>They encode for antibiotic resistance mechanisms. (C)</p> Signup and view all the answers

What happens during the preparation of an agar plate after transferring the soil sample?

<p>The plate must be flipped upside down before incubation. (A)</p> Signup and view all the answers

What is the role of the yellow rod when plating bacteria on an agar plate?

<p>To spread the liquid evenly over the surface of the plate. (B)</p> Signup and view all the answers

What defines a β-lactam antibiotic?

<p>It contains a β-lactam ring structure. (B)</p> Signup and view all the answers

What is the primary purpose of DNA isolation?

<p>To remove cellular proteins and RNA (C)</p> Signup and view all the answers

Which component does NOT belong in a master mix used for PCR?

<p>Ethidium bromide (B)</p> Signup and view all the answers

What is the significance of Taq polymerase in PCR applications?

<p>It is heat-resistant and can function at high temperatures (C)</p> Signup and view all the answers

Which step in the PCR process involves the separation of DNA strands?

<p>Denaturing stage (D)</p> Signup and view all the answers

What is the main function of a PCR machine?

<p>To replicate DNA through cycles of temperature changes (B)</p> Signup and view all the answers

How is gel electrophoresis used in molecular biology?

<p>To separate DNA fragments by size (D)</p> Signup and view all the answers

Why is a DNA ladder used in gel electrophoresis?

<p>To calibrate the size of DNA fragments (B)</p> Signup and view all the answers

What does a thinner band in electrophoresis indicate regarding DNA samples?

<p>There are fewer DNA molecules present (D)</p> Signup and view all the answers

What must be done to load samples into gel electrophoresis wells accurately?

<p>Keep the plunger depressed while placing the tip in the well (C)</p> Signup and view all the answers

What happens during the annealing stage of PCR?

<p>Primers attach to the template DNA (C)</p> Signup and view all the answers

Flashcards

M Stage

The stage in the cell cycle where the cell divides. It includes two processes: mitosis and cytokinesis.

Mitosis

The process of nuclear division where the duplicated chromosomes are distributed to two daughter nuclei, resulting in two identical sets.

Incomplete Dominance

A genetic inheritance pattern where the heterozygote phenotype is a blend of the two homozygous phenotypes.

Codominance

A genetic inheritance pattern where both alleles are expressed equally, resulting in both traits being visible.

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Sex Linkage

Traits influenced by genes located on sex chromosomes (X or Y), leading to different inheritance patterns in males and females.

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True-breeding

Organisms that always produce offspring with the same trait when self-fertilized.

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Monohybrid Cross

A cross between two individuals differing in only one trait.

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Allele

An alternative form of a gene.

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Homozygous

Having two identical alleles for a specific trait.

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Heterozygous

Having two different alleles for a specific trait.

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Gene Locus

The specific location of a gene on a chromosome.

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Antibiotics

Natural or synthetic substances that slow or stop bacterial growth. They can target different bacterial processes like enzyme activity, cell wall formation, protein synthesis, or DNA replication.

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β-lactam Antibiotics

A type of antibiotic that works by interfering with bacterial cell wall formation. They contain a β-lactam ring, which is crucial for their function.

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β-lactamases

Enzymes produced by bacteria that break down β-lactam antibiotics, making them ineffective.

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BLA Genes

Genes responsible for producing β-lactamases, allowing bacteria to resist β-lactam antibiotics.

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Agar Plate Labeling

Labeling the bottom of the agar plate with information such as your name, date, medium type, and organism.

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Soil Sample Extraction

Collecting soil samples using a sterile container to prevent contamination. Scoop up a small amount of soil using the container lip.

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Bacteria Plating

Transferring a small amount of bacterial suspension onto an agar plate and spreading it evenly using a sterile rod.

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Micropipette Use

A precise tool used to transfer small, specific volumes of liquid. It involves setting the desired volume, attaching a sterile tip, and carefully transferring the liquid.

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Soil Smoothie

A mixture of distilled water and soil, used to suspend bacteria for further analysis.

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Supernatant

The liquid layer that forms on top of the soil suspension after it settles.

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DNA Isolation

A technique for isolating DNA by removing cellular proteins and RNA.

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Master Mix

A mixture containing enzymes and precursors used in polymerase chain reaction (PCR) techniques.

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Taq DNA Polymerase

A heat-resistant enzyme used in PCR that helps build new DNA strands.

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PCR (Polymerase Chain Reaction)

A technique for rapidly copying DNA fragments by repeatedly cycling through denaturation, annealing, and extension.

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PCR Machine

A device used to amplify DNA for various applications like DNA fingerprinting and pathogen detection.

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Gel Electrophoresis

A technique used to separate DNA fragments based on their size using an electric current.

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DNA Ladder

A standard mixture of DNA fragments of known sizes used to determine the size of unknown DNA fragments in gel electrophoresis.

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Loading Samples into Gel Electrophoresis Wells

The process of carefully placing DNA samples into wells of a gel electrophoresis apparatus using a micropipette.

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Interpreting Gel Electrophoresis Results

Analyzing the banding patterns of DNA fragments in a gel electrophoresis experiment to determine their size and presence.

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Controls in Gel Electrophoresis

Samples of known DNA fragments used to ensure the experiment is working properly and to provide a reference point for comparison.

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Haploid

Having only one set of chromosomes, typically found in gametes (sperm and egg cells) produced during meiosis.

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Diploid

Having two sets of chromosomes, typically found in most body cells (somatic cells).

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Synapsis

The pairing of homologous chromosomes during meiosis. This allows for crossing over, exchanging genetic material.

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Crossing Over

The exchange of genetic material between homologous chromosomes during synapsis. This creates new combinations of genes and contributes to genetic diversity.

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Centromere

The region of a chromosome where sister chromatids are attached, playing a crucial role in chromosome movement during cell division.

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Stem Cells

Undifferentiated cells that have the potential to develop into specialized cell types. They are vital for tissue growth and repair, and they hold promise for therapeutic applications.

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Therapeutic Cloning

The process of producing human tissues using stem cells, potentially for treating diseases or injuries.

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Cell Cycle Checkpoints

Control points within the cell cycle that ensure the proper execution of each phase, preventing errors and ensuring cell division occurs only under appropriate conditions.

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Study Notes

Meiosis

  • Produces four haploid cells, which are non-identical.
  • Requires two nuclear divisions.
  • Chromosomes synapse and cross over.
  • Centromeres survive Anaphase I.
  • Chromosome number is halved.
  • Produces four daughter nuclei.
  • Daughter cells are genetically different from the parent and each other.
  • Used only for sexual reproduction.

Mitosis

  • Requires one nuclear division.
  • Chromosomes do not synapse or cross over.
  • Centromeres dissolve in mitotic anaphase.
  • Chromosome number is preserved.
  • Produces two daughter nuclei.
  • Daughter cells are genetically identical to the parent and each other.
  • Used for asexual reproduction and growth.
  • Found in mammalian organs and stem cells.
  • Allows cells to retain the ability to divide.
  • Occurs in red bone marrow to produce various blood cells.

Cell Cycle and Checkpoints: Interphase

  • Cell performs usual functions during interphase.
  • Time varies by cell type.
  • Interphase consists of G1, S, and G2 phases.
  • G1 checkpoint : Checks for proper DNA replication before continuing. Apoptosis occurs if DNA is damaged and cannot be repaired.
  • Mitosis checkpoint : Checks for proper duplication of DNA. Apoptosis occurs if DNA is damaged and cannot be repaired.
  • G2 checkpoint : Checks for proper DNA duplication. Apoptosis occurs if DNA is damaged and cannot be repaired.
  • M checkpoint : Checks if chromosomes are properly aligned before mitosis proceeds.

M (Mitotic) Stage

  • Includes Mitosis (nuclear division).
  • Daughter chromosomes are distributed by mitotic spindle to two daughter nuclei.
  • Includes Cytokinesis (division of cytoplasm).
  • Results in two genetically identical daughter cells.

Incomplete Dominance

  • Heterozygote phenotype is intermediate between either homozygote.
  • Homozygous red = red phenotype.
  • Homozygous white = white phenotype.
  • Phenotype reveals genotype without a test cross.

Dihybrid Cross

  • Multiple alleles occur when there are more than two versions of a gene (alleles) for a trait in a population.
  • Each trait is controlled by two alleles.

Dominant and Recessive Alleles

  • Dominant allele masks expression of recessive allele.
  • Alleles occur on homologous pairs of chromosomes at particular gene loci.
  • Homozygous = identical alleles.
  • Heterozygous = different alleles.
  • Used "true-breeding" (homozygous) plants.
  • Chose varieties that differed in only one trait (monohybrid cross).

Codominance

  • Two versions of a gene/alleles are expressed equally resulting in the appearance of both traits.

Sex Linkage

  • Traits influenced by sex chromosomes (X or Y).

Antibiotics

  • Antibiotics (e.g., penicillin, streptomycin) are natural or synthetic molecules which stop bacterial growth and survival.
  • They inhibit enzymes in metabolic pathways, cell wall synthesis, protein synthesis, or DNA synthesis.
  • B-lactams inhibit bacterial activity by bonding to penicillin binding proteins.
  • B-Lactamase enzymes are produced by bacteria to deactivate B-lactam antibiotics.

B-lactam Antibiotics in Soil Samples

  • Labelling agar plates with samples, date, medium type, and sample information.
  • Extracting soil samples using various tools.
  • Plating soil bacteria on agar plates.
  • Extracting bacteria from agar plates.

DNA Isolation

  • DNA Isolation is a technique used to remove cellular proteins, and RNA to isolate DNA.
  • Master mix contains precursors, enzymes, dNTPs, MgCl2, Taq polymerase, pH buffer, in nuclease-free water.

PCR (Polymerase Chain Reaction)

  • Used to replicate DNA, detect DNA, DNA fingerprinting, forensic analysis, and pathogen detection.
  • Steps include Denaturing, Annealing, and Extending.

Gel Electrophoresis

  • Separates DNA fragments based on size using an electric current through gel.
  • DNA ladder is used to determine the size of DNA molecules.

Antibiotic Resistance

  • Bacteria develop resistance due to gene changes. This change allows bacteria to resist antibiotic drugs.

Lab Handout Questions

  • All questions from lab handouts are fair game.

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