Cell Biology Paper 1
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Questions and Answers

Which cell structure is unique to plant cells and not present in animal cells?

  • Cell wall (correct)
  • Plasma membrane
  • Chloroplasts
  • Cytoskeleton

What is the primary function of chloroplasts in plant cells?

  • Perform cellular respiration
  • Regulate transport across the cell membrane
  • Use light to create food (glucose) (correct)
  • Contain waste materials

Which process is common to both prokaryotic and eukaryotic cells?

  • Cellular respiration
  • Transcription of DNA into RNA (correct)
  • Translation of RNA into proteins
  • Cell wall formation

What is a key characteristic that distinguishes prokaryotic cells from eukaryotic cells?

<p>Presence of a membrane-bound nucleus (C)</p> Signup and view all the answers

Why is cellular diversity important in building complex organisms like the human body?

<p>To allow for better adaptation of cells (D)</p> Signup and view all the answers

Which organelle is exclusive to eukaryotic cells and plays a crucial role in generating energy?

<p>Mitochondria (C)</p> Signup and view all the answers

What is the function of pili and flagella structures in prokaryotic cells?

<p>Allow for movement (A)</p> Signup and view all the answers

What is a key characteristic of microtubules in the cytoskeleton?

<p>They consist of dimers of α- and γ-Tubulin (D)</p> Signup and view all the answers

What role do Microtubule Organizing Centres (MTOCs) play in cells?

<p>Replicate to form two distinct MTOCs before cell division starts (D)</p> Signup and view all the answers

How do the proteins forming the cytoskeleton resemble LEGO pieces?

<p>They assemble into a wide range of structures by fitting together (D)</p> Signup and view all the answers

Why is the dynamic nature of the cytoskeleton essential for its functions?

<p>For polymers and regulatory proteins to be constantly in flux (A)</p> Signup and view all the answers

Which type of microtubules is primarily involved in connecting sister chromatids to the spindle apparatus during cell division?

<p>Kinetochore Microtubules (C)</p> Signup and view all the answers

In what aspect are microfilaments different from microtubules?

<p>Microfilaments are made of actin subunits while microtubules are made of tubulin subunits. (C)</p> Signup and view all the answers

What role do intermediate filaments play in the cell's response to mechanical stresses?

<p>They cross-link with other cytoskeletal elements like microtubules and microfilaments. (B)</p> Signup and view all the answers

What is the primary function of actin filaments in endocytosis?

<p>Creating a contractile ring around the cell's 'waist' (C)</p> Signup and view all the answers

How do pathogens manipulate the host cytoskeleton?

<p>Through interactions with extracellular receptors and intracellular signaling pathways (A)</p> Signup and view all the answers

What distinguishes intermediate filaments from microtubules and microfilaments?

<p>Intermediate filaments are highly divergent in sequence and molecular weight. (C)</p> Signup and view all the answers

How does ATP provide energy in cellular reactions?

<p>By transferring a phosphate group to another molecule in phosphorylation (D)</p> Signup and view all the answers

What is the primary role of mitochondria in the cell?

<p>To generate ATP through cellular processes (B)</p> Signup and view all the answers

In cellular respiration, what is the role of the Krebs cycle?

<p>To produce ATP from glucose in the presence of oxygen (C)</p> Signup and view all the answers

How is ATP used to power cellular processes?

<p>By phosphorylating molecules to activate them (D)</p> Signup and view all the answers

What are the three major clinical features associated with Alpers’ Syndrome?

<p>Severe epilepsy, loss of developmental skills, and liver failure (A)</p> Signup and view all the answers

Which organelle is responsible for synthesizing lipids, aiding in detoxification of drugs, and metabolizing carbohydrates?

<p>Rough Endoplasmic Reticulum (A)</p> Signup and view all the answers

What is the correct sequence for the process of proteins being secreted from the cell?

<p>Proteins have a secretion signal, mRNA binds ribosome, protein starts to be produced, mRNA export (B)</p> Signup and view all the answers

Which cell organelle is responsible for receiving transport vesicles containing proteins from the Rough Endoplasmic Reticulum and modifying these proteins?

<p>Golgi Apparatus (A)</p> Signup and view all the answers

What is the key enzyme in glycolysis that regulates the speed of the pathway based on the energy needs of the cell?

<p>Phosphofructokinase (C)</p> Signup and view all the answers

Which phase of glycolysis involves the conversion of three-carbon molecules to pyruvate, generating ATP and NADH?

<p>Energy-Releasing Phase (B)</p> Signup and view all the answers

What are the net products generated from one round of glycolysis?

<p>2 ATP, 2 NADH, 2 pyruvate (A)</p> Signup and view all the answers

Which process occurs in the absence of oxygen and is essential for ATP production when oxygen is scarce?

<p>Glycolysis (D)</p> Signup and view all the answers

Which molecule is formed when acetyl-CoA combines with oxaloacetate during the Krebs cycle?

<p>Citrate (C)</p> Signup and view all the answers

What is the primary function of the Krebs cycle in cellular metabolism?

<p>Energy production through oxidation (C)</p> Signup and view all the answers

What is the primary role of protein complexes in the electron transport chain?

<p>Transfer electrons and pump protons (C)</p> Signup and view all the answers

What is the function of Ubiquinone(Q) and Cytochrome c in the electron transport chain?

<p>Shuttle electrons between complexes (C)</p> Signup and view all the answers

What happens when cyanide acts on complex IV in the electron transport chain?

<p>Stoppage of electron transfer to oxygen (C)</p> Signup and view all the answers

How does the electron transport chain utilize the energy from redox reactions?

<p>Drive the synthesis of ATP by pumping protons (A)</p> Signup and view all the answers

What is one of the major effects of poisoning by cyanide or carbon monoxide on cellular metabolism?

<p>Elevated lactate levels in the blood (B)</p> Signup and view all the answers

What is the primary function of ATP in cellular processes?

<p>Transfers a phosphate group to another molecule through phosphorylation (C)</p> Signup and view all the answers

Which statement accurately describes ATP's role as the 'energy currency' of the cell?

<p>ATP is essential for various cell functions and serves as a universal energy carrier (C)</p> Signup and view all the answers

How does ATP contribute to cellular respiration?

<p>ATP causes conformational changes in proteins (A)</p> Signup and view all the answers

In what way does ATP differ from adenosine diphosphate (ADP) in cellular energy processes?

<p>ATP is hydrolyzed to generate ADP and energy (A)</p> Signup and view all the answers

What is the primary function of the central dogma of molecular biology?

<p>Regulation of gene expression (A)</p> Signup and view all the answers

Where does the process of translation occur within a cell?

<p>Cytosol (C)</p> Signup and view all the answers

What is the primary function of the ribosome in a cell?

<p>Protein synthesis (D)</p> Signup and view all the answers

In which cellular organelle does the final stage of gene expression (translation) take place?

<p>Cytosol (B)</p> Signup and view all the answers

Which component carries the growing polypeptide chain during the elongation phase of protein synthesis?

<p>tRNA in P site (D)</p> Signup and view all the answers

What distinguishes free floating ribosomes from membrane-bound ribosomes in a cell?

<p>Synthesis of different types of proteins (D)</p> Signup and view all the answers

What provides stability to the DNA molecule and contributes to its structural integrity?

<p>Phosphodiester bonds (C)</p> Signup and view all the answers

Which groove, formed by the staggered arrangement of nitrogenous bases along the double helix, is wider and deeper?

<p>Major groove (B)</p> Signup and view all the answers

In DNA replication, what enzyme catalyzes the formation of phosphodiester bonds?

<p>DNA polymerase (A)</p> Signup and view all the answers

Which structure is made up of DNA tightly coiled many times around proteins called histones?

<p>Chromosomes (B)</p> Signup and view all the answers

What is the primary role of histones in the process of deacetylation?

<p>Inhibiting transcription (D)</p> Signup and view all the answers

Which type of microtubules is primarily involved in connecting sister chromatids to the spindle apparatus during cell division?

<p>'A' microtubules (B)</p> Signup and view all the answers

'Triplet code' refers to a sequence of how many nucleotides on a DNA or RNA molecule?

<p>Three (B)</p> Signup and view all the answers

'Huntington’s disease' primarily targets which structures in a process called deacetylation?

<p>Histones (C)</p> Signup and view all the answers

'Replication' in cellular division specifically results in the formation of how many double-stranded DNA molecules in the nucleus?

<p>2 (B)</p> Signup and view all the answers

'Triplet code' controls the production of which cellular component in the cytoplasm of the cell?

<p>'C' amino acids (B)</p> Signup and view all the answers

What is the information content more accessible in major groove because what is exposed along the double helix?

<p>Nitrogenous bases (D)</p> Signup and view all the answers

What is the primary function of the Krebs cycle in cellular metabolism?

<p>Generation of NADH and FADH2 for ATP production (D)</p> Signup and view all the answers

Which enzyme plays a crucial role in regulating the speed of glycolysis based on the energy needs of the cell?

<p>Phosphofructokinase (A)</p> Signup and view all the answers

In the absence of oxygen, what is the primary pathway for ATP production in cells?

<p>Glycolysis (B)</p> Signup and view all the answers

What happens when cyanide acts on complex IV in the electron transport chain?

<p>It stops all electrons being passed to oxygen (C)</p> Signup and view all the answers

Which phase of glycolysis involves using two ATP molecules to convert one molecule of glucose into two three-carbon molecules of glyceraldehyde-3-phosphate?

<p>Energy-Requiring Phase (B)</p> Signup and view all the answers

How do pathogens manipulate the host cytoskeleton?

<p>By altering actin filament polymerization (B)</p> Signup and view all the answers

What is the purpose of the proton gradient created in the electron transport chain?

<p>To drive ATP synthesis by ATP synthase (A)</p> Signup and view all the answers

What is the net ATP yield per molecule of glucose metabolized through glycolysis?

<p>+2 ATP (B)</p> Signup and view all the answers

What role do Microtubule Organizing Centres (MTOCs) play in cells?

<p>Cytoskeleton structure (D)</p> Signup and view all the answers

What distinguishes intermediate filaments from microtubules and microfilaments?

<p>Their structural stability and diverse protein composition (B)</p> Signup and view all the answers

Why is cellular diversity important in building complex organisms like the human body?

<p>To enable the specialization of cells for different tasks (D)</p> Signup and view all the answers

What are the three major clinical features associated with Alpers’ Syndrome?

<p>Seizures, developmental regression, liver failure (A)</p> Signup and view all the answers

Which organelle aids in the detoxification of drugs and other toxins?

<p>Rough Endoplasmic Reticulum (A)</p> Signup and view all the answers

What is the primary function of the Golgi Apparatus in a cell?

<p>Protein modification and sorting (A)</p> Signup and view all the answers

Which molecule is formed by millions of nucleotides joined together in a long chain?

<p>DNA (B)</p> Signup and view all the answers

What is the role of Pentose Sugar in a nucleotide?

<p>Provides a sugar component to the nucleotide (C)</p> Signup and view all the answers

Which pair always forms bonds in DNA by pairing up?

<p>(Guanine, Thymine) (B)</p> Signup and view all the answers

Which chemical bonds link adjacent nucleotides in a DNA strand?

<p>Phosphodiester bonds (A)</p> Signup and view all the answers

What is the major difference between purines and pyrimidines in nitrogenous bases?

<p>Number of heterocyclic rings they contain (D)</p> Signup and view all the answers

What is the primary role of Ribosomes in protein synthesis?

<p>Synthesize proteins based on mRNA instructions (A)</p> Signup and view all the answers

Which type of cells are unicellular in nature?

<p>Prokaryotic cells (D)</p> Signup and view all the answers

What is a major difference in DNA structure between prokaryotic and eukaryotic cells?

<p>Eukaryotic DNA is circular with proteins (C)</p> Signup and view all the answers

Which organelle is responsible for motility in eukaryotic cells by waving cilia and flagellin?

<p>Centrioles (C)</p> Signup and view all the answers

During which phase of the cell cycle does DNA replication occur?

<p>Interphase (A)</p> Signup and view all the answers

Which type of cell division involves binary fission?

<p>Binary fission exclusively (C)</p> Signup and view all the answers

How does DNA differ in packaging between euchromatin and heterochromatin?

<p>Euchromatin is loosely packed, while heterochromatin is tightly packed (D)</p> Signup and view all the answers

What enzyme catalyzes the formation of phosphodiester bonds during DNA replication?

<p>DNA polymerase (A)</p> Signup and view all the answers

What is the term for the process by which DNA is copied to produce an identical replica?

<p>Replication (A)</p> Signup and view all the answers

Which structure in eukaryotic cells contains the genetic material and directs cellular activities?

<p>Nucleus (D)</p> Signup and view all the answers

What is the primary function of cytoskeletons in eukaryotic cells?

<p>Cellular transport (B)</p> Signup and view all the answers

Which type of ribosomes are present in prokaryotic cells?

<p>70S (C)</p> Signup and view all the answers

In which phase of the cell cycle does DNA condense into tightly packed chromosomes?

<p>Mitosis (C)</p> Signup and view all the answers

Which organelle in plant cells contains DNA in addition to the nucleus?

<p>Chloroplast (C)</p> Signup and view all the answers

What is the primary method of cell motility in prokaryotic cells?

<p>Rigid rotating flagellum (A)</p> Signup and view all the answers

What is the primary function of the centrosome in eukaryotic cells during the late G2 phase?

<p>Serves as the main microtubule organizing center of the cell (B)</p> Signup and view all the answers

What happens to cohesin proteins during anaphase of mitosis?

<p>They lead to the separation of sister chromatids (A)</p> Signup and view all the answers

Which phase of the eukaryotic cell cycle marks the time when the cell has stopped dividing and has left the cycle?

<p>Resting phase (Gap 0) (B)</p> Signup and view all the answers

During which phase of mitosis do chromosomes begin to condense and the nucleolus disappears?

<p>Prophase (B)</p> Signup and view all the answers

During which phase of meiosis do homologous chromosomes line up at the metaphase plate and can undergo crossing over?

<p>Metaphase I (B)</p> Signup and view all the answers

What is the major difference between the process of anaphase in mitosis compared to anaphase I in meiosis?

<p>Sister chromatids separate in mitosis, homologous chromosomes separate in meiosis (A)</p> Signup and view all the answers

What structural feature allows for the formation of chiasmata during meiosis?

<p>Synaptonemal complex (C)</p> Signup and view all the answers

Which process contributes to genetic diversity among gametes during meiosis?

<p>Crossing over (D)</p> Signup and view all the answers

What is the significance of cells resulting from meiosis II being haploid?

<p>It prepares for fertilization to restore diploidy (C)</p> Signup and view all the answers

In which phase of meiosis does the nuclear envelope begin to reform around the separated chromosomes?

<p>Telophase I (A)</p> Signup and view all the answers

Which process is involved in the production of functional gene products like proteins and functional RNAs?

<p>Translation (A)</p> Signup and view all the answers

What is the discipline focused on sequencing, mapping, analyzing, and comparing genomes known as?

<p>Genomics (C)</p> Signup and view all the answers

At the molecular level, which component of a gene encodes and directs the synthesis of proteins, tRNA, rRNA, or other structural RNAs?

<p>DNA (A)</p> Signup and view all the answers

What is the primary function of the Fundamental Dogma in biology?

<p>To link DNA replication and RNA transcription (A)</p> Signup and view all the answers

What is the role of release factors in translation termination?

<p>Assisting in the recognition of stop codons (C)</p> Signup and view all the answers

What occurs in the E site of the ribosome during translation termination?

<p>Drift out of empty tRNA (C)</p> Signup and view all the answers

What defines a polyribosome?

<p>A cluster of ribosomes linked by tRNAs (B)</p> Signup and view all the answers

During which phase of translation does the A site act as the landing site for the next tRNA with an anticodon complementary to the exposed codon on mRNA?

<p>Elongation (B)</p> Signup and view all the answers

What is the primary function of the 5' capping process during RNA processing?

<p>Addition of a methyl-guanosine cap at the 5' end (C)</p> Signup and view all the answers

Which phase of gene expression involves the addition of ~200 adenines to the 3' end of pre-mRNA?

<p>RNA processing (C)</p> Signup and view all the answers

What is the role of promoters in transcription?

<p>Serving as recognition points for RNA polymerase binding (C)</p> Signup and view all the answers

Which phase of translation involves the binding of the 40S ribosomal subunit to mRNA?

<p>Initiation (A)</p> Signup and view all the answers

What is the function of aminoacyl-tRNA synthetases in protein synthesis?

<p>Adding amino acids to tRNA molecules (D)</p> Signup and view all the answers

What is the primary role of splicing in RNA processing?

<p>Removing non-coding introns and joining coding exons (D)</p> Signup and view all the answers

Which structure in tRNA is responsible for carrying a specific amino acid?

<p>'CCA' tail (B)</p> Signup and view all the answers

'Universal Genetic Code' refers to a common language for translating nucleotide sequences into what during protein synthesis?

<p>'Amino acid sequences' (C)</p> Signup and view all the answers

What is the function of ribosomes in protein synthesis?

<p>Making proteins by translating mRNA sequences (C)</p> Signup and view all the answers

What is the primary function of the operator in an operon?

<p>Halts transcription by binding to DNA (A)</p> Signup and view all the answers

In prokaryotic cells, what determines whether an operon is inducible or repressible?

<p>The ability of small molecules to turn it off (A)</p> Signup and view all the answers

Which DNA-binding protein inhibits the expression of genes by binding to the operator?

<p>Repressor (C)</p> Signup and view all the answers

How does the operon differ from a single gene in prokaryotic cells?

<p>An operon operates from a single promoter, while a single gene has multiple promoters (B)</p> Signup and view all the answers

What is the key difference between Rho-dependent and Rho-independent transcription termination strategies?

<p>Formation of a hairpin structure in the RNA transcript (C)</p> Signup and view all the answers

What is the role of the Shine-Dalgarno sequence in protein synthesis?

<p>Complementing the 16S rRNA in the ribosome (C)</p> Signup and view all the answers

How does Rho factor contribute to transcription termination in prokaryotes?

<p>Pulling the RNA transcript and DNA apart (B)</p> Signup and view all the answers

What is the function of the 16S rRNA in prokaryotic ribosomes during translation initiation?

<p>Binds to mRNA Shine-Dalgarno sequence (C)</p> Signup and view all the answers

What happens when C and G rich region on DNA template is approached during transcription?

<p>Hairpin formation in the mRNA (A)</p> Signup and view all the answers

Why does the presence of U nucleotides after the hairpin structure aid in transcription termination?

<p>Weakens mRNA-DNA interaction (D)</p> Signup and view all the answers

What happens when an inducer molecule is present in the trp operon of E.coli?

<p>It binds to the repressor protein, changing its conformation. (D)</p> Signup and view all the answers

What role does the lacY gene play in the lac operon of E.coli?

<p>It is involved in transporting lactose into the cell. (D)</p> Signup and view all the answers

Which statement accurately describes the regulation of the Trp Operon in E.coli?

<p>The repressor proteins are unable to bind to the operator when tryptophan is present. (D)</p> Signup and view all the answers

Which region of DNA is essential for initiating transcription in prokaryotes?

<p>Pribnow box (B)</p> Signup and view all the answers

What is the function of the lacI gene in the lac operon regulation?

<p>Codes for an allosteric repressor protein (C)</p> Signup and view all the answers

What happens in E.coli when lactose is absent in terms of the lac operon?

<p>The repressor protein is inactive allowing transcription. (A)</p> Signup and view all the answers

"What is the primary function of RNA polymerase during transcription initiation?"

<p>&quot;Transcribes mRNA from DNA template.&quot; (C)</p> Signup and view all the answers

"What purpose does suspending repressible enzymes serve in cells?"

<p>&quot;Allows energy allocation elsewhere by halting unnecessary processes.&quot; (C)</p> Signup and view all the answers

"What differentiates inducible enzymes from repressible enzymes regarding cellular role?"

<p>&quot;Inducible enzymes break down nutrients while repressible enzymes synthesise end products.&quot; (A)</p> Signup and view all the answers

What is the primary factor that heavily influences the movement of charged particles in an electric field during gel electrophoresis?

<p>Size of the molecules (C)</p> Signup and view all the answers

In gel electrophoresis, what is the role of the buffer used when loading the gel?

<p>Provide electrolytes to facilitate current movement (A)</p> Signup and view all the answers

What is the purpose of loading DNA ladder (reference of Molecular weight marker) in gel electrophoresis?

<p>To identify the size of DNA bands (A)</p> Signup and view all the answers

At which end of the gel is the Cathode (-) placed during electrophoresis?

<p>Top loading end (A)</p> Signup and view all the answers

What is the purpose of using 70% ethanol in the biological safety cabinet?

<p>To disinfect items inside the cabinet (D)</p> Signup and view all the answers

Why is it important to warm cell culture media and additives to 37°C?

<p>To maintain the viability of cells (B)</p> Signup and view all the answers

What is the expected outcome of using trypan blue in viability staining?

<p>Viable cells will have intact membranes and not be colorized (B)</p> Signup and view all the answers

Why should movements within and outside the biological safety cabinet not be rapid?

<p>To maintain the sterile airflow inside the cabinet (B)</p> Signup and view all the answers

What is the significance of labeling materials used for cell culture with initials, date, and additives?

<p>To track and ensure proper identification of materials (B)</p> Signup and view all the answers

How does ethanol (EtOH) contribute to viability staining using trypan blue?

<p>It kills bacteria by rupturing their cell membranes (B)</p> Signup and view all the answers

What is the primary function of sphingomyelin in the cell membrane?

<p>Participates in signaling pathways (B)</p> Signup and view all the answers

Which characteristic best describes integral membrane proteins in the cell membrane?

<p>Permanently embedded within the membrane (D)</p> Signup and view all the answers

What is the main role of carbohydrates in the cell membrane?

<p>Assist in cell recognition (A)</p> Signup and view all the answers

Which adhesion molecule is involved in cell-cell adhesion?

<p>Cadherin (D)</p> Signup and view all the answers

What is the primary function of CAMs (Cell Adhesion Molecules) in cellular communication?

<p>Maintain physical contact between cells (C)</p> Signup and view all the answers

How do phospholipids contribute to the selective permeability of the cell membrane?

<p>By allowing certain molecules to diffuse across the membrane (D)</p> Signup and view all the answers

What factor heavily influences the movement of charged particles in an electric field in gel electrophoresis?

<p>New charge of molecules (C)</p> Signup and view all the answers

In gel electrophoresis, what is the primary function of the gel matrix made out of agarose?

<p>Retard the movement of molecules based on density (C)</p> Signup and view all the answers

What is the buffer's role in gel electrophoresis?

<p>Provide electrolytes to facilitate current movement (D)</p> Signup and view all the answers

When loading the gel in gel electrophoresis, what is the purpose of mixing the DNA sample with loading buffer/dye containing a high-density molecule like sucrose?

<p>Help DNA sink to the bottom of the well (C)</p> Signup and view all the answers

What is the purpose of warming cell culture media and additives to 37ËšC in a water bath when preparing fresh media?

<p>To promote cell growth (B)</p> Signup and view all the answers

Why is it essential to label all materials used for cell culture with initials, date, and additives?

<p>For proper identification and tracking (B)</p> Signup and view all the answers

In viability staining using trypan blue, why are live cells with intact membranes not colorized?

<p>The dye does not penetrate live cells (C)</p> Signup and view all the answers

What is the function of ethanol in treating cells by rupturing the cell membrane?

<p>To sterilize by disrupting bacterial membranes (B)</p> Signup and view all the answers

Why is it important to avoid rapid movements within and outside the biological safety cabinet?

<p>To maintain sterile airflow (C)</p> Signup and view all the answers

What is the reason behind seeding low cell count/density leading to cell death?

<p>Inadequate nutrient availability (C)</p> Signup and view all the answers

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