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Questions and Answers
What is the purpose of the special fluid used in the cell counting method described in the text?
What is the purpose of the special fluid used in the cell counting method described in the text?
- To stain the nuclei of white cells
- To hemolyze red cells
- To count the cells more accurately
- To dilute the blood sample (correct)
What is the function of the Neubauer counting chamber in the cell counting method described in the text?
What is the function of the Neubauer counting chamber in the cell counting method described in the text?
- To stain the nuclei of white cells
- To hemolyze red cells
- To dilute the blood sample
- To count the cells (correct)
What is the difference between the white cells pipette and the red cells pipette in the cell counting method described in the text?
What is the difference between the white cells pipette and the red cells pipette in the cell counting method described in the text?
- The white cells pipette dilutes the blood sample less than the red cells pipette
- The white cells pipette is used to count red cells, while the red cells pipette is used to count white cells
- The white cells pipette dilutes blood 1/20, while the red cells pipette dilutes blood 1/200 (correct)
- The white cells pipette dilutes the blood sample more than the red cells pipette
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Study Notes
- Method involves diluting blood with special fluid
- Special pipette with white bead used for white cells
- Counting chamber used to count cells
- Neubauer counting chamber has square area with fine lines
- Dilution fluid made of glacial acetic acid and methyl blue/gentian violet
- Acetic acid hemolysis red cells but not white cells
- Gentian violet stains nuclei of white cells for easier recognition
- Special coverslip used
- White cells pipette dilutes blood 1/20, red cells pipette dilutes 1/200
- Accurate dilution important for accurate cell count
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