3 Questions
What is the purpose of the special fluid used in the cell counting method described in the text?
To dilute the blood sample
What is the function of the Neubauer counting chamber in the cell counting method described in the text?
To count the cells
What is the difference between the white cells pipette and the red cells pipette in the cell counting method described in the text?
The white cells pipette dilutes blood 1/20, while the red cells pipette dilutes blood 1/200
Study Notes
- Method involves diluting blood with special fluid
- Special pipette with white bead used for white cells
- Counting chamber used to count cells
- Neubauer counting chamber has square area with fine lines
- Dilution fluid made of glacial acetic acid and methyl blue/gentian violet
- Acetic acid hemolysis red cells but not white cells
- Gentian violet stains nuclei of white cells for easier recognition
- Special coverslip used
- White cells pipette dilutes blood 1/20, red cells pipette dilutes 1/200
- Accurate dilution important for accurate cell count
Test your knowledge on Hemocytometer, the tool used to count blood cells. Learn about the dilution process involving a special fluid, pipettes, and counting chambers. Understand the importance of accurate dilution and the use of acetic acid and gentian violet in the process. This quiz will cover the technical aspects of Hemocytometer and help you improve your skills in counting blood cells accurately.
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