Podcast
Questions and Answers
Which of the following best describes the primary function of electron microscopy in cell biology?
Which of the following best describes the primary function of electron microscopy in cell biology?
- To measure the electrical activity of cells
- To observe living cells in real-time
- To identify the chemical composition of cellular components
- To visualize cellular ultrastructure at high resolution (correct)
Which cellular component is typically NOT visible using light microscopy without specific staining or treatments?
Which cellular component is typically NOT visible using light microscopy without specific staining or treatments?
- Nucleus
- Mitochondria
- Ribosomes (correct)
- Cell membrane
What is the role of chromatin in the nucleus?
What is the role of chromatin in the nucleus?
- To produce ribosomes
- To generate energy for the cell
- To synthesize proteins
- To package DNA and regulate gene expression (correct)
Which of the following is a function of the cell membrane?
Which of the following is a function of the cell membrane?
Euchromatin differs from heterochromatin in that euchromatin is:
Euchromatin differs from heterochromatin in that euchromatin is:
Which of the following structures is NOT directly involved in protein synthesis?
Which of the following structures is NOT directly involved in protein synthesis?
Which type of microscopy is most suitable for observing the 3D surface structure of a cell?
Which type of microscopy is most suitable for observing the 3D surface structure of a cell?
What property of lipids allows them to spontaneously form bilayers in water?
What property of lipids allows them to spontaneously form bilayers in water?
A cell with a high density of ribosomes is most likely specialized for:
A cell with a high density of ribosomes is most likely specialized for:
Which unit of measurement is most appropriate for describing the size of a typical ribosome?
Which unit of measurement is most appropriate for describing the size of a typical ribosome?
What is the functional significance of nuclear pores?
What is the functional significance of nuclear pores?
Which of the following most accurately describes the appearance of the cell membrane under TEM?
Which of the following most accurately describes the appearance of the cell membrane under TEM?
How does the structure of the rough endoplasmic reticulum (RER) support its function?
How does the structure of the rough endoplasmic reticulum (RER) support its function?
Consider a cell that is actively secreting large quantities of proteins. Which organelle would you expect to be particularly prominent in this cell?
Consider a cell that is actively secreting large quantities of proteins. Which organelle would you expect to be particularly prominent in this cell?
What is the primary function of the nucleolus?
What is the primary function of the nucleolus?
Which of the following is a key difference between transmission electron microscopy (TEM) and scanning electron microscopy (SEM)?
Which of the following is a key difference between transmission electron microscopy (TEM) and scanning electron microscopy (SEM)?
If a researcher is studying the dynamic movements of proteins within the cell membrane, which technique would be most appropriate?
If a researcher is studying the dynamic movements of proteins within the cell membrane, which technique would be most appropriate?
A cell is observed to have an unusually large amount of smooth endoplasmic reticulum (SER). This likely indicates that the cell is specialized for:
A cell is observed to have an unusually large amount of smooth endoplasmic reticulum (SER). This likely indicates that the cell is specialized for:
What is the structural relationship between the nuclear envelope and the endoplasmic reticulum?
What is the structural relationship between the nuclear envelope and the endoplasmic reticulum?
Which of the following is the correct order of steps involved in preparing a sample for transmission electron microscopy?
Which of the following is the correct order of steps involved in preparing a sample for transmission electron microscopy?
Which property primarily defines the 'electron-dense' regions seen in a transmission electron micrograph?
Which property primarily defines the 'electron-dense' regions seen in a transmission electron micrograph?
What would be the effect on a cell if its nuclear pores were non-functional?
What would be the effect on a cell if its nuclear pores were non-functional?
Under a light microscope, a tissue sample stained with hematoxylin and eosin (H&E) shows cells with intensely basophilic nuclei. What does this suggest about the cells?
Under a light microscope, a tissue sample stained with hematoxylin and eosin (H&E) shows cells with intensely basophilic nuclei. What does this suggest about the cells?
If a cell's membrane suddenly became permeable to large ions, which of the following processes would be most directly affected?
If a cell's membrane suddenly became permeable to large ions, which of the following processes would be most directly affected?
A researcher is studying the interaction between a transmembrane protein and the extracellular matrix. Which microscopic technique would provide the MOST direct visualization of this interaction?
A researcher is studying the interaction between a transmembrane protein and the extracellular matrix. Which microscopic technique would provide the MOST direct visualization of this interaction?
What is the functional consequence of having glycoproteins and glycolipids on the cell surface?
What is the functional consequence of having glycoproteins and glycolipids on the cell surface?
Which of the following best explains why electron microscopes have a much higher resolution than light microscopes?
Which of the following best explains why electron microscopes have a much higher resolution than light microscopes?
A cell is exposed to a toxin that disrupts the function of the Golgi apparatus. Which of the following cellular processes would be most directly affected?
A cell is exposed to a toxin that disrupts the function of the Golgi apparatus. Which of the following cellular processes would be most directly affected?
Which of the following is most likely to be observed in cells specialized for detoxification?
Which of the following is most likely to be observed in cells specialized for detoxification?
Which type of cells can nerve cells be up to 100µm in diameter?
Which type of cells can nerve cells be up to 100µm in diameter?
Which processes take place in the cytoplasm?
Which processes take place in the cytoplasm?
Protein synthesis takes place in the
Protein synthesis takes place in the
1000 nanometers = ?
1000 nanometers = ?
Which of the following can be seen using light and electron microscopy?
Which of the following can be seen using light and electron microscopy?
Which of the following is NOT a component of the nucleus?
Which of the following is NOT a component of the nucleus?
Which microscope does not require a lens?
Which microscope does not require a lens?
What is the function of the Golgi apparatus?
What is the function of the Golgi apparatus?
When viewing a cell under a light microscope, which of the following structures provides the most important clue to the cell's overall shape and function?
When viewing a cell under a light microscope, which of the following structures provides the most important clue to the cell's overall shape and function?
Which of these microscopes uses electrons?
Which of these microscopes uses electrons?
What type of cell modification is cell to cell attachments?
What type of cell modification is cell to cell attachments?
Which property does not belong to a cell?
Which property does not belong to a cell?
Which of the following statements about the cell membrane under the light microscope (LM) is most accurate?
Which of the following statements about the cell membrane under the light microscope (LM) is most accurate?
What distinguishes transmission electron microscopy (TEM) from scanning electron microscopy (SEM) in terms of sample preparation?
What distinguishes transmission electron microscopy (TEM) from scanning electron microscopy (SEM) in terms of sample preparation?
A researcher notices that a particular cell type has a nucleus with a very irregular shape and a high proportion of heterochromatin. What can the researcher infer about cells with this type of nucleus?
A researcher notices that a particular cell type has a nucleus with a very irregular shape and a high proportion of heterochromatin. What can the researcher infer about cells with this type of nucleus?
Flashcards
Electron Microscopy
Electron Microscopy
A technique that uses electrons to create a magnified image of a specimen, revealing ultrastructure.
Transmission Electron Microscopy (TEM)
Transmission Electron Microscopy (TEM)
A type of microscopy where electrons pass through a specimen to form an image.
Scanning Electron Microscopy (SEM)
Scanning Electron Microscopy (SEM)
A type of microscopy where electrons scan the surface of a specimen to create an image.
Cell Membrane
Cell Membrane
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Cytoskeleton
Cytoskeleton
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Nucleus
Nucleus
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Mitochondria
Mitochondria
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Golgi Apparatus
Golgi Apparatus
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Endoplasmic Reticulum
Endoplasmic Reticulum
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Ribosomes
Ribosomes
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Vesicles
Vesicles
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Microvilli
Microvilli
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Cilia
Cilia
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Nuclear Envelope
Nuclear Envelope
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Chromatin
Chromatin
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Nucleolus
Nucleolus
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Nanometer (nm)
Nanometer (nm)
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Micrometer (µm)
Micrometer (µm)
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Millimeter (mm)
Millimeter (mm)
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Lipid Bilayer
Lipid Bilayer
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Amphipathic
Amphipathic
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Integral Membrane Proteins
Integral Membrane Proteins
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Peripheral Membrane Proteins
Peripheral Membrane Proteins
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Glycoproteins
Glycoproteins
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Glycolipids
Glycolipids
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Transmembrane Channel Protein
Transmembrane Channel Protein
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Intracellular Space
Intracellular Space
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Extracellular Space
Extracellular Space
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DNA
DNA
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mRNA
mRNA
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Transcription
Transcription
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Translation
Translation
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Nuclear Pores
Nuclear Pores
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Heterochromatin
Heterochromatin
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Euchromatin
Euchromatin
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Study Notes
Introduction to Electron Microscopy
- Electron microscopy is explored and how to interpret electron micrographs.
- Light and electron microscopy can visualize different cell components.
- Part 1 describes how light and electron microscopes work in simple terms.
Cell Components
- Key cell components include the cell membrane, cytoskeleton, and nucleus.
- The cytoskeleton consists of microfilaments, intermediate filaments, and microtubules.
- The nucleus contains chromosomes as chromatin, including euchromatin and heterochromatin, and the nucleolus.
- Other components: mitochondria, Golgi apparatus, endoplasmic reticulum (smooth and rough), ribosomes, and vesicles.
- Modifications to the cell membrane include microvilli, cilia, and cell-to-cell attachments.
Microscopy
- An electron micrograph of an eosinophil reveals details compared to a light micrograph.
- Light microscope utilizes a lamp, condenser lens, specimen, objective lens, ocular lens, and projection lens to produce an image.
- The transmission electron microscope uses a cathode, anode, condenser lens, scanning coil, scanning beam, electron detector, and television screen to create an image.
- The scanning electron microscope also includes an electronic amplifier.
Transmission Electron Microscopy
- Electrons hitting the phosphor screen glow brightly and are classified as electron lucent.
- Areas where electrons do not hit have no glow and are classified as electron dense.
Scanning Electron Microscopy
- Scanning electron microscopy involves an electron gun, condenser lenses, scan coils, objective lens, specimen stub, X-ray detector, and secondary electron detector.
Units of Measurement
- Units of measurement:
- 10 Angstroms = 1.0 nanometer (nm)
- 1000 nanometers = 1.0 micrometer (µm)
- 1000 micrometers (microns) = 1.0 millimeter (mm)
- A red blood cell (RBC) is approximately 7.5 µm in diameter.
- Neuron cell bodies can be up to 100 µm in diameter.
Electron Micrographs
- Electron micrographs help understand what organelles exist, their appearance, individual functions, and collective function.
- Understanding size and magnification is important for interpreting electron micrographs.
Cell Membrane
- Part 2 focuses on histology of the cell membrane: structure, function, and composition.
- Lipid bilayers form plasma membranes and surround cellular organelles.
- Lipids contain a hydrophobic end and a hydrophilic end, making them "amphipathic."
- Lipids spontaneously form lipid bilayers in water.
- The cell membrane regulates the passage of material into and out of the cell.
Proteins/Glycoproteins within Cell Membranes
- Proteins/Glycoproteins mean they appear eosinophilic under light microscopy and electron-dense under electron microscopy.
- Resolution limits the ability to see membranes under light microscopy.
Histology of the Nucleus
- Part 3 examines the histology of the nucleus, covering normal microscopic appearance, structure, and function.
- Under normal light microscopy, you can expect to find the nucleus, chromatin (pale or densely staining basophilia), chromosomes, and nucleolus.
- The shape of the nucleus serves as a guide to the shape of the cell under light microscopy, important when examining epithelia as a tissue.
- LM staining patterns distinguish and indicate cell types and activities.
Nucleus Composition Under EM
- Under normal electron microscopy, expect to find the nucleus and nuclear membrane.
- There are also nuclear pores.
- Chromatin, including euchromatin and heterochromatin is visible.
- Chromosomes and the nucleolus can also be found under EM.
Nuclear Pores
- Nuclear pores exist for transcription and translation.
- During transcription, DNA is converted to mRNA inside the nucleus.
- The mRNA then exits through the nuclear pores into the cytoplasm for translation.
- During translation, the mRNA combines with ribosomes to synthesize proteins.
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