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Questions and Answers
What are the two major parts of a cell?
What are the two major parts of a cell?
Nucleus and cytoplasm
What is the historic scientific theory that living organisms are made up of cells called?
What is the historic scientific theory that living organisms are made up of cells called?
Cell theory
Which of the following scientists contributed to cell theory?
Which of the following scientists contributed to cell theory?
The cell is the basic unit of structure and organization of organisms.
The cell is the basic unit of structure and organization of organisms.
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Cells arise from pre-existing cells.
Cells arise from pre-existing cells.
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Energy flow (metabolism and biochemistry) occurs outside of cells.
Energy flow (metabolism and biochemistry) occurs outside of cells.
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All cells are basically the same in chemical composition in organisms of similar species.
All cells are basically the same in chemical composition in organisms of similar species.
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What allowed the classification of cells into eukaryotes and prokaryotes?
What allowed the classification of cells into eukaryotes and prokaryotes?
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Which of the following belong to the eukaryotes?
Which of the following belong to the eukaryotes?
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Prokaryotes have a typical nucleus and subcellular components.
Prokaryotes have a typical nucleus and subcellular components.
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A cell has three major components. Which of the following is NOT a component?
A cell has three major components. Which of the following is NOT a component?
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What is the thin membrane that envelops a cell called?
What is the thin membrane that envelops a cell called?
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The plasma membrane defines the periphery of the cell separating the cell contents from the surrounding environment.
The plasma membrane defines the periphery of the cell separating the cell contents from the surrounding environment.
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The plasma membrane is composed of a lipid bilayer and protein embedded in it.
The plasma membrane is composed of a lipid bilayer and protein embedded in it.
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The plasma membrane has highly permeable properties.
The plasma membrane has highly permeable properties.
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Receptor proteins on the membrane transmit signals into the cell.
Receptor proteins on the membrane transmit signals into the cell.
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What is the fluid portion of the cytoplasm in which particles are suspended called?
What is the fluid portion of the cytoplasm in which particles are suspended called?
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Which of the following is NOT an important organelle suspended in the cytoplasm?
Which of the following is NOT an important organelle suspended in the cytoplasm?
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The endoplasmic reticulum is a network of interconnecting membranes enclosing channels or cisternae.
The endoplasmic reticulum is a network of interconnecting membranes enclosing channels or cisternae.
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The endoplasmic reticulum is continuous from the outer nuclear envelope to the inner plasma membrane.
The endoplasmic reticulum is continuous from the outer nuclear envelope to the inner plasma membrane.
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The rough endoplasmic reticulum is where complex lipid and steroid are synthesized.
The rough endoplasmic reticulum is where complex lipid and steroid are synthesized.
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The rough endoplasmic reticulum appears rough due to the attachment of ribosomes.
The rough endoplasmic reticulum appears rough due to the attachment of ribosomes.
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Drug detoxification occurs in the endoplasmic reticulum.
Drug detoxification occurs in the endoplasmic reticulum.
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The nucleus is the most important organelle.
The nucleus is the most important organelle.
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All mammalian cells contain a nucleus except mature red blood cells (RBCs).
All mammalian cells contain a nucleus except mature red blood cells (RBCs).
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The nucleus is where the DNA, the genetic material of the cell, is stored.
The nucleus is where the DNA, the genetic material of the cell, is stored.
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The DNA consists of the complete set of genes (genome) of the organism only in prokaryotes.
The DNA consists of the complete set of genes (genome) of the organism only in prokaryotes.
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The nucleus governs the cell because it contains the DNA which is the chemical basis of the genes.
The nucleus governs the cell because it contains the DNA which is the chemical basis of the genes.
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The genetic material, DNA, forms chromatin by complexing with histone protein.
The genetic material, DNA, forms chromatin by complexing with histone protein.
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Chromatin is further organized into chromosomes.
Chromatin is further organized into chromosomes.
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The nucleolus is where protein synthesis takes place.
The nucleolus is where protein synthesis takes place.
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DNA replication takes place in the nucleolus.
DNA replication takes place in the nucleolus.
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Mitochondria are spherical oval or rod-shaped bodies in the cytoplasm.
Mitochondria are spherical oval or rod-shaped bodies in the cytoplasm.
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Mitochondria are involved in the breakdown of food stuff and release energy.
Mitochondria are involved in the breakdown of food stuff and release energy.
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Mitochondria store energy as ATP for the use of the cell in other metabolic processes.
Mitochondria store energy as ATP for the use of the cell in other metabolic processes.
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Mitochondria have only one membrane.
Mitochondria have only one membrane.
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The enzymes of the electron transport chain (ETC), where energy is trapped, are found in the inner membrane folds of mitochondria.
The enzymes of the electron transport chain (ETC), where energy is trapped, are found in the inner membrane folds of mitochondria.
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Cytochrome P450, involved in steroid synthesis, is found in the mitochondria.
Cytochrome P450, involved in steroid synthesis, is found in the mitochondria.
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Mature red blood cells contain mitochondria.
Mature red blood cells contain mitochondria.
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Mammalian cells have specific DNA called mitochondrial DNA, distinct from the DNA in the nucleus.
Mammalian cells have specific DNA called mitochondrial DNA, distinct from the DNA in the nucleus.
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Lysosomes are large organelles where foreign particles are digested.
Lysosomes are large organelles where foreign particles are digested.
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Lysosomes are comparable to incinerators where waste is burnt.
Lysosomes are comparable to incinerators where waste is burnt.
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Enzymes released from lysosomes can cause tissue damage.
Enzymes released from lysosomes can cause tissue damage.
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Lysosomes help in the destruction of bacteria and other foreign bodies and the removal of excessive secretory products in the cells of the glands.
Lysosomes help in the destruction of bacteria and other foreign bodies and the removal of excessive secretory products in the cells of the glands.
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The Golgi complex or Golgi apparatus is a network of flat, smooth membranes and vesicles.
The Golgi complex or Golgi apparatus is a network of flat, smooth membranes and vesicles.
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The main function of the Golgi apparatus is to sort, package, mature, and release protein already synthesized.
The main function of the Golgi apparatus is to sort, package, mature, and release protein already synthesized.
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The Golgi aparattus is present in all cells, including red blood cells.
The Golgi aparattus is present in all cells, including red blood cells.
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Peroxisomes contain enzymes such as peroxidases and catalases, which are concerned with the metabolism of peroxides.
Peroxisomes contain enzymes such as peroxidases and catalases, which are concerned with the metabolism of peroxides.
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Peroxisomes are also capable of carrying out β-oxidation of fatty acids.
Peroxisomes are also capable of carrying out β-oxidation of fatty acids.
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The cytoskeleton of eukaryotic cells contains networks of protein filaments.
The cytoskeleton of eukaryotic cells contains networks of protein filaments.
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The cytoskeleton helps fix organelles in the cell.
The cytoskeleton helps fix organelles in the cell.
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The cytoskeleton is a rigid permanent framework of the cell.
The cytoskeleton is a rigid permanent framework of the cell.
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Which of the following is NOT a primary protein filament of the cytoskeleton?
Which of the following is NOT a primary protein filament of the cytoskeleton?
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Microfilaments are about 5 nm in diameter and are made up of protein actin.
Microfilaments are about 5 nm in diameter and are made up of protein actin.
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Microfilaments form a meshwork just underlying the plasma membrane of cells, referred to as the cell cortex.
Microfilaments form a meshwork just underlying the plasma membrane of cells, referred to as the cell cortex.
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Microfilaments disappear as cell motility increases but do not reappear when motility stops.
Microfilaments disappear as cell motility increases but do not reappear when motility stops.
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Microfilaments help maintain the shape of the cell.
Microfilaments help maintain the shape of the cell.
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Microfilaments help cellular movement.
Microfilaments help cellular movement.
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Microtubules are cylindrical tubes, 20 to 25 nm in diameter, made up of protein tubulin.
Microtubules are cylindrical tubes, 20 to 25 nm in diameter, made up of protein tubulin.
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Microtubules are necessary for the formation and function of the mitotic spindle.
Microtubules are necessary for the formation and function of the mitotic spindle.
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Microtubules prevent tubules of the endoplasmic reticulum from collapsing.
Microtubules prevent tubules of the endoplasmic reticulum from collapsing.
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Microtubules are major components of axons and dendrites.
Microtubules are major components of axons and dendrites.
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Intermediate filaments are so called because their diameter (10 nm) is intermediate between that of microfilaments (5 nm) and microtubules (25 nm).
Intermediate filaments are so called because their diameter (10 nm) is intermediate between that of microfilaments (5 nm) and microtubules (25 nm).
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Intermediate filaments are formed from fibrous proteins which varies with different tissue type.
Intermediate filaments are formed from fibrous proteins which varies with different tissue type.
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Intermediate filaments play a role in cell-to-cell attachment.
Intermediate filaments play a role in cell-to-cell attachment.
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Intermediate filaments help stabilize the epithelium.
Intermediate filaments help stabilize the epithelium.
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Intermediate filaments provide strength and rigidity to axons.
Intermediate filaments provide strength and rigidity to axons.
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The cytoskeleton gives cells their characteristic shape and form.
The cytoskeleton gives cells their characteristic shape and form.
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The cytoskeleton provides attachment points for organelles, fixing their location in cells.
The cytoskeleton provides attachment points for organelles, fixing their location in cells.
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The cytoskeleton makes communication between parts of the cell possible.
The cytoskeleton makes communication between parts of the cell possible.
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The cytoskeleton helps separate chromosomes during cell division.
The cytoskeleton helps separate chromosomes during cell division.
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The internal movement of cell organelles and cell locomotion would be possible without the cytoskeleton.
The internal movement of cell organelles and cell locomotion would be possible without the cytoskeleton.
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The cytoskeleton acts as a track on which cells can move organelles, chromosomes, and other things.
The cytoskeleton acts as a track on which cells can move organelles, chromosomes, and other things.
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One of the functions of the plasma membrane is to regulate the passage of a variety of small molecules across it.
One of the functions of the plasma membrane is to regulate the passage of a variety of small molecules across it.
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Biological membranes are permeable membranes.
Biological membranes are permeable membranes.
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The movement of molecules across a membrane is restricted because of size, charge, or solubility.
The movement of molecules across a membrane is restricted because of size, charge, or solubility.
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The permeability of substances across the cell membrane is dependent on their solubility in lipids and not on their molecular size.
The permeability of substances across the cell membrane is dependent on their solubility in lipids and not on their molecular size.
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Water-soluble compounds are generally permeable and do not require carrier-mediated transport.
Water-soluble compounds are generally permeable and do not require carrier-mediated transport.
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An important function of the membrane is to withhold unwanted molecules while permitting the entry of molecules necessary for cellular metabolism.
An important function of the membrane is to withhold unwanted molecules while permitting the entry of molecules necessary for cellular metabolism.
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Transport mechanisms are classified into two types. Which of the following is NOT a transport mechanism?
Transport mechanisms are classified into two types. Which of the following is NOT a transport mechanism?
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Passive transport requires energy in the form of ATP.
Passive transport requires energy in the form of ATP.
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The direction of passive transport is always from a region of higher concentration to one of lower concentration.
The direction of passive transport is always from a region of higher concentration to one of lower concentration.
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Active transport is always from a region of lower concentration to one of higher concentration.
Active transport is always from a region of lower concentration to one of higher concentration.
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Active transport is classified into two types according to the source of energy used.
Active transport is classified into two types according to the source of energy used.
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Primary active transport is directly coupled with hydrolysis of ATP.
Primary active transport is directly coupled with hydrolysis of ATP.
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Secondary active transport uses an energy generated by an electrochemical gradient.
Secondary active transport uses an energy generated by an electrochemical gradient.
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Secondary active transport is classified into two types: co-transport and counter-transport.
Secondary active transport is classified into two types: co-transport and counter-transport.
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Co-transport is when both substances move simultaneously across the membrane in the same direction.
Co-transport is when both substances move simultaneously across the membrane in the same direction.
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Counter-transport is when both substances move simultaneously across the membrane in opposite directions.
Counter-transport is when both substances move simultaneously across the membrane in opposite directions.
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The process by which cells take up large molecules is called exocytosis, and the process by which cells release large molecules from the cells to the outside is called endocytosis.
The process by which cells take up large molecules is called exocytosis, and the process by which cells release large molecules from the cells to the outside is called endocytosis.
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There are two types of endocytosis: pinocytosis (cellular drinking) and phagocytosis (cellular eating).
There are two types of endocytosis: pinocytosis (cellular drinking) and phagocytosis (cellular eating).
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Pinocytosis is the only process by which most macromolecules, such as proteins, polysaccharides, and polynucleotides, can enter cells.
Pinocytosis is the only process by which most macromolecules, such as proteins, polysaccharides, and polynucleotides, can enter cells.
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Molecules first attach to specific receptors on the surface of the membrane before undergoing pinocytosis.
Molecules first attach to specific receptors on the surface of the membrane before undergoing pinocytosis.
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Phagocytosis involves the ingestion of large particles such as viruses, bacteria, cells, tissue debris, or a dead cell.
Phagocytosis involves the ingestion of large particles such as viruses, bacteria, cells, tissue debris, or a dead cell.
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Phagocytosis only occurs in specialized cells, such as macrophages and white blood cells.
Phagocytosis only occurs in specialized cells, such as macrophages and white blood cells.
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Phagocytosis occurs in the same way as pinocytosis.
Phagocytosis occurs in the same way as pinocytosis.
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To obtain purified preparations of organelles, tissue is first carefully broken up in a homogenizing apparatus using a hypertonic solution.
To obtain purified preparations of organelles, tissue is first carefully broken up in a homogenizing apparatus using a hypertonic solution.
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Sucrose solution is used because it is not metabolized in most tissues.
Sucrose solution is used because it is not metabolized in most tissues.
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Sucrose solution does not pass through membranes readily.
Sucrose solution does not pass through membranes readily.
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Sucrose solution does not cause interorganelles to swell.
Sucrose solution does not cause interorganelles to swell.
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The homogenate is centrifuged at a series of increasing centrifugal force.
The homogenate is centrifuged at a series of increasing centrifugal force.
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The subcellular organelles, which differ in size and specific gravity, sediment at different rates and can be isolated from the homogenate by differential centrifugation.
The subcellular organelles, which differ in size and specific gravity, sediment at different rates and can be isolated from the homogenate by differential centrifugation.
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The dense nuclei are sedimented last, followed by the mitochondria.
The dense nuclei are sedimented last, followed by the mitochondria.
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After all the particulate matter has been removed, the soluble remnant is the cytosol.
After all the particulate matter has been removed, the soluble remnant is the cytosol.
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Organelles of similar sedimentation coefficients can be easily separated by differential centrifugation.
Organelles of similar sedimentation coefficients can be easily separated by differential centrifugation.
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Mitochondria isolated by this method are not contaminated with lysosomes and peroxisomes.
Mitochondria isolated by this method are not contaminated with lysosomes and peroxisomes.
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Organelles can be separated by isopycnic centrifugation technique.
Organelles can be separated by isopycnic centrifugation technique.
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The purity of isolated subcellular fractions is assessed by analyzing marker enzymes.
The purity of isolated subcellular fractions is assessed by analyzing marker enzymes.
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Marker enzymes are the enzymes located exclusively in a particular fraction.
Marker enzymes are the enzymes located exclusively in a particular fraction.
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Analysis of marker enzymes confirms the identity of the isolated fraction and indicates the degree of contamination with other organelles.
Analysis of marker enzymes confirms the identity of the isolated fraction and indicates the degree of contamination with other organelles.
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Isolated mitochondria have a high specific activity of cytochrome oxidase, but low catalase and acid phosphatase.
Isolated mitochondria have a high specific activity of cytochrome oxidase, but low catalase and acid phosphatase.
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The low activity of catalase and acid phosphatase in isolated mitochondria is due to contamination with peroxisomes and lysosomes.
The low activity of catalase and acid phosphatase in isolated mitochondria is due to contamination with peroxisomes and lysosomes.
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The plasma membrane contains a variety of enzymes, including 5'-nucleotidase and Na+-K+ ATPase.
The plasma membrane contains a variety of enzymes, including 5'-nucleotidase and Na+-K+ ATPase.
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The nucleus contains DNA polymerase, RNA polymerase, and protein synthesis enzymes.
The nucleus contains DNA polymerase, RNA polymerase, and protein synthesis enzymes.
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The endoplasmic reticulum contains high levels of glucose-6-phosphatase.
The endoplasmic reticulum contains high levels of glucose-6-phosphatase.
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Golgi bodies contain galactosyl transferase.
Golgi bodies contain galactosyl transferase.
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Lysosomes contain high levels of acid phosphatase.
Lysosomes contain high levels of acid phosphatase.
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Mitochondria contain succinate dehydrogenase and cytochrome C oxidase.
Mitochondria contain succinate dehydrogenase and cytochrome C oxidase.
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Peroxisomes contain high levels of catalase.
Peroxisomes contain high levels of catalase.
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The cytosol contains lactate dehydrogenase and glucose-6-phosphate dehydrogenase.
The cytosol contains lactate dehydrogenase and glucose-6-phosphate dehydrogenase.
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Study Notes
Cell Biology: Structure & Function
- Cells are the fundamental structural and functional units of all living organisms.
- The major components of a cell are the nucleus and cytoplasm.
- Cell theory postulates that all living organisms are composed of one or more cells, cells are the basic unit of structure and organization of organisms, cells arise from pre-existing cells, and the activity of an organism depends on the total activity of independent cells.
Cell Definition and Theory
- Cells are the basic units of structure and function for all living things.
- Energy flow (metabolism and biochemistry) happens within cells.
- Cells contain DNA within their nucleus and cytoplasm.
- Cells in similar species have basically the same chemical composition.
- Eukaryotes have a nucleus enclosed by a nuclear membrane.
- Eukaryotic cells are generally larger than prokaryotic cells.
- Prokaryotes lack a membrane-bound nucleus. Examples include bacteria and blue-green algae.
Structure and Functions of a Cell and its Subcellular Components
- A typical eukaryotic cell has three main components: the plasma membrane, cytoplasm, and nucleus.
- The plasma membrane separates the cell's contents from the surrounding environment.
- It has selective permeability which is regulated by membrane transport proteins.
- Membrane also acts as barrier to many charged or polar compounds.
- Proteins and other components are interspersed within the lipid bilayer.
The Fluid Mosaic Model of Cell Membrane
- Membrane structure is described as a fluid mosaic model.
- Proposed by Nicholson and Singer in 1972.
- The bilayer is fluid due to an appropriate mixture of saturated and unsaturated fatty acids in the phospholipids.
- Proteins are interspersed within the lipid bilayer.
- Peripheral proteins are on the membrane surface and connected by ionic bonds.
- Integral membrane proteins span the bilayer, connected by hydrophobic bonds (Van der Waals forces).
- The cholesterol content influences membrane fluidity.
Cytoplasm and its Organelles
- Cytoplasm is the internal volume enclosed by the plasma membrane.
- Cytosol is the clear fluid in which other cell components are suspended.
- Six vital organelles in the cytoplasm are: endoplasmic reticulum, Golgi apparatus, lysosomes, peroxisomes, mitochondria, and the nucleus.
Endoplasmic Reticulum
- The endoplasmic reticulum is a network of interconnected membranes.
- It extends from the outer nuclear membrane to the plasma membrane.
- Consists of rough ER (with ribosomes, protein synthesis) and smooth ER (lipid and steroid synthesis; drug detoxification).
Nucleus
- All cells (except mature red blood cells) have a nucleus.
- The nucleus houses DNA (genetic material).
- DNA organizes into chromatin, then chromosomes.
- Nucleolus is an area of the nucleus involved in RNA synthesis.
- DNA Replication occurs in the nucleus.
Mitochondria
- Mitochondria are the powerhouses of the cell.
- They release energy from food oxidation.
- The process stores energy as ATP.
- Inner membrane has folded cristae containing enzymes of the electron transport chain (ETC).
- Some DNA is found within mitochondria.
Lysosomes
- These are organelles involved in digesting foreign substances.
- They contain enzymes that break down substances.
Golgi Complex
- The Golgi apparatus packages and processes proteins for secretion.
- A network of flat, smooth membranes and vesicles.
Peroxisome
- Peroxisomes house enzymes to metabolize peroxides and some fatty acids.
The Cytoskeleton
- The cytoskeleton gives cells their shape and structure.
- Interacts with other components, including the plasma membrane.
- Contains three filament types: microfilaments, microtubules, and intermediate filaments.
- Microfilaments are involved in cell contractility and support of the cell cortex.
- Microtubules maintain cell shape.
- Intermediate filaments provide strength.
Functions of Cytoskeleton
- Maintains cell shape and form.
- Provides attachment points for organelles.
- Facilitates communication between cell parts.
- Necessary for cell division processes.
- Supports cellular movement and muscle contraction,
- Carries organelles, chromosomes and other things
Membrane Transport
- Plasma membranes regulate the passage of molecules.
- Permeability depends on size, charge, and solubility.
- Water-soluble compounds often require carrier-mediated transport.
- Transport mechanisms classified into passive and active transport.
Passive Transport
- Passive transport (e.g., diffusion) moves molecules down a concentration gradient without energy input.
- Simple diffusion refers to lipid-soluble molecules passing directly through the membrane.
- Facilitated diffusion requires carrier proteins to transport molecules across the membrane.
Active Transport
- Active transport moves molecules against a concentration gradient which requires energy.
- Primary active transport uses ATP directly to drive the transport. An example is the Na+-K+ pump (sodium-potassium pump).
- Secondary active transport uses the electrochemical gradient created by primary active transport to move molecules. (Symport or Antiport)
Transport of Macromolecules Across the Plasma Membrane
- Endocytosis involves taking in large molecules into the cell.
- Pinocytosis is a process of cellular uptake of fluid and fluid contents.
- Phagocytosis involves ingestion of large particles.
- Exocytosis is the release of particles from the cell.
Cell Fractionation
- Separating organelles for study by differential centrifugation (using different speeds and forces).
- Isopycnic methods can separate similar density components.
- Purity of obtained parts is verified with analyses of marker enzymes. These characteristic enzymes provide identification of the components.
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Description
Explore the fundamental concepts of cell biology, including the definitions and theories surrounding cell structure and function. This quiz covers important topics such as cell theory, eukaryotic and prokaryotic cells, and the components of cells that contribute to their organization and energy flow.