Cell Biology Quiz

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Questions and Answers

What is the primary function of ribosomes?

  • Storage of genetic material
  • Energy production
  • Cell replication
  • Protein synthesis (correct)

Which structure is responsible for modifying, sorting, and packaging proteins?

  • Mitochondria
  • Endoplasmic Reticulum
  • Golgi Apparatus (correct)
  • Nucleus

What is one key difference between the rough ER and the smooth ER?

  • Rough ER synthesizes carbohydrates while smooth ER does not
  • Rough ER is involved in detoxification while smooth ER is not
  • Smooth ER is involved in protein folding while rough ER is not
  • Rough ER contains ribosomes while smooth ER does not (correct)

Which structure in eukaryotic cells is primarily involved in assembling ribosomes?

<p>Nucleolus (A)</p> Signup and view all the answers

What are chloroplasts primarily responsible for in plant cells?

<p>Photosynthesis (A)</p> Signup and view all the answers

Which organelle is crucial for the production of ATP?

<p>Mitochondria (C)</p> Signup and view all the answers

What role does the cytoskeleton play in a cell?

<p>Structural support and movement (A)</p> Signup and view all the answers

What is the main function of lysosomes in animal cells?

<p>Digestion of waste materials (B)</p> Signup and view all the answers

What is the primary function of microtubules in a cell?

<p>Help in cell division and movement (D)</p> Signup and view all the answers

Which type of intercellular junction prevents fluid leakage between cells?

<p>Tight junctions (D)</p> Signup and view all the answers

Which transport mechanism requires energy to move substances against a concentration gradient?

<p>Active transport (D)</p> Signup and view all the answers

What is a characteristic of meiosis that differentiates it from mitosis?

<p>It reduces the chromosome number by half (B)</p> Signup and view all the answers

In which phase of meiosis do homologous chromosomes separate?

<p>Anaphase I (B)</p> Signup and view all the answers

What role does cholesterol play in the plasma membrane?

<p>Helps maintain membrane stability (C)</p> Signup and view all the answers

What structure forms during Prophase I of meiosis allowing genetic recombination?

<p>Tetrad (C)</p> Signup and view all the answers

Which of the following is a characteristic of the extracellular matrix (ECM) in animal cells?

<p>Provides structural and biochemical support (D)</p> Signup and view all the answers

Which of the following is NOT a type of passive transport?

<p>Endocytosis (A)</p> Signup and view all the answers

What is the role of the gene in a cell?

<p>Serves as a blueprint for biological traits (B)</p> Signup and view all the answers

What happens during crossing over in meiosis?

<p>Homologous chromosomes exchange genetic material (C)</p> Signup and view all the answers

What defines a cell as haploid?

<p>Contains one set of chromosomes (B)</p> Signup and view all the answers

What occurs during Telophase II of meiosis?

<p>Four genetically distinct haploid cells are formed (D)</p> Signup and view all the answers

What is the main function of the Golgi apparatus?

<p>Modification, sorting, and packaging of proteins (C)</p> Signup and view all the answers

Which structure contains digestive enzymes that help break down waste materials?

<p>Lysosomes (D)</p> Signup and view all the answers

What role do peroxisomes play in the cell?

<p>Breakdown of fatty acids and detoxification (B)</p> Signup and view all the answers

Which component of the cytoskeleton provides mechanical strength and stability to the cell?

<p>Intermediate filaments (C)</p> Signup and view all the answers

What is the purpose of vacuoles in plant cells?

<p>Storage of water and nutrients, and maintaining turgor pressure (B)</p> Signup and view all the answers

How does mitosis ensure genetic consistency in daughter cells?

<p>By maintaining the same number of chromosomes as the parent cell (B)</p> Signup and view all the answers

What occurs during the G₁ checkpoint of the cell cycle?

<p>Resource evaluation for cell division (D)</p> Signup and view all the answers

What distinguishes Meiosis II from mitosis?

<p>Involvement of haploid cells without DNA replication (D)</p> Signup and view all the answers

Which organelle is involved in photosynthesis?

<p>Chloroplast (D)</p> Signup and view all the answers

What forms during cytokinesis in plant cells?

<p>Cell plate (C)</p> Signup and view all the answers

During which phase do sister chromatids separate?

<p>Anaphase (D)</p> Signup and view all the answers

What is the role of spindle fibers in cell division?

<p>Attaching to chromosomes to ensure proper alignment and separation (C)</p> Signup and view all the answers

What structure is primarily responsible for transporting materials within the cell?

<p>Vesicles (C)</p> Signup and view all the answers

What is the end product of mitosis in terms of chromosome number?

<p>Two daughter cells with the same number of chromosomes as the parent cell (B)</p> Signup and view all the answers

Which process occurs in the mitochondrion to generate ATP?

<p>Cellular Respiration (A)</p> Signup and view all the answers

What is the main role of the thylakoid membranes in chloroplasts?

<p>Light energy absorption and ATP synthesis (C)</p> Signup and view all the answers

Which of the following is not a product of the light reactions in photosynthesis?

<p>Glucose (C)</p> Signup and view all the answers

What is the function of the ribosome in a cell?

<p>Translation of mRNA into proteins (C)</p> Signup and view all the answers

In which cellular compartment does the Calvin cycle take place?

<p>Stroma of chloroplasts (B)</p> Signup and view all the answers

What is the final electron acceptor in oxidative phosphorylation?

<p>Oxygen (O₂) (B)</p> Signup and view all the answers

Which component increases the surface area of the inner mitochondrial membrane?

<p>Cristae (C)</p> Signup and view all the answers

What is the primary purpose of chemiosmosis during photosynthesis?

<p>pumping protons to create a gradient (B)</p> Signup and view all the answers

Which of the following correctly describes the structure of the plasma membrane?

<p>Bilayer of phospholipids with embedded proteins (A)</p> Signup and view all the answers

How does the arrangement of chlorophyll in plants affect photosynthesis?

<p>Excites electrons and transfers energy efficiently (D)</p> Signup and view all the answers

What role do the ribosomal subunits play during protein synthesis?

<p>Facilitate the assembly of amino acids into proteins (B)</p> Signup and view all the answers

Which function is not performed by the smooth endoplasmic reticulum (SER)?

<p>Synthesis of proteins (B)</p> Signup and view all the answers

What mechanism leads to the production of NADPH during photosynthesis?

<p>Electron transport and photophosphorylation (D)</p> Signup and view all the answers

What role do transcription factors play in eukaryotic gene expression?

<p>They help RNA polymerase bind to DNA. (A)</p> Signup and view all the answers

What role does the nucleolus play within the nucleus?

<p>Synthesis of ribosomal RNA (rRNA) (B)</p> Signup and view all the answers

During RNA processing, which of the following modifications occurs?

<p>Addition of a protective cap only. (A)</p> Signup and view all the answers

What initiates translation at the ribosome?

<p>The first tRNA pairing with the start codon. (D)</p> Signup and view all the answers

Which of the following correctly describes the process of glycolysis?

<p>It breaks down glucose into pyruvate, producing ATP. (C)</p> Signup and view all the answers

What is the primary function of the citric acid cycle?

<p>To oxidize pyruvate and release carbon dioxide. (D)</p> Signup and view all the answers

How does energy coupling work in cellular processes?

<p>It involves linking exergonic and endergonic reactions. (A)</p> Signup and view all the answers

What is the role of chlorophyll in photosynthesis?

<p>It absorbs sunlight to produce ATP and NADPH. (D)</p> Signup and view all the answers

Which of the following is NOT a method of regulating gene expression?

<p>Epigenetic changes affecting DNA sequence. (C)</p> Signup and view all the answers

What characterizes cancer cells in terms of metabolic processes?

<p>They often alter their energy metabolism for rapid growth. (A)</p> Signup and view all the answers

What is the function of introns in eukaryotic mRNA?

<p>They are removed during splicing to form mature mRNA. (B)</p> Signup and view all the answers

What is the primary method of reproduction in prokaryotic cells?

<p>Binary fission (C)</p> Signup and view all the answers

Which component is crucial for spindle formation during mitosis?

<p>Centrosome (C)</p> Signup and view all the answers

During which phase of mitosis do chromosomes align at the metaphase plate?

<p>Metaphase (D)</p> Signup and view all the answers

Which process releases energy by breaking ATP bonds?

<p>Hydrolysis (B)</p> Signup and view all the answers

What type of energy is associated with the motion of molecules?

<p>Kinetic energy (D)</p> Signup and view all the answers

Which law states that energy cannot be created or destroyed, only transformed?

<p>First Law of Thermodynamics (D)</p> Signup and view all the answers

Which enzyme is responsible for adding free nucleotides during DNA replication?

<p>DNA Polymerase (D)</p> Signup and view all the answers

What is produced when mRNA is translated at the ribosome?

<p>Polypeptide (C)</p> Signup and view all the answers

Which structure attaches chromosomes to spindle fibers during cell division?

<p>Kinetochore (D)</p> Signup and view all the answers

During which process does DNA unwind and break hydrogen bonds between base pairs?

<p>Replication (C)</p> Signup and view all the answers

In which part of eukaryotic cells does transcription occur?

<p>Nucleus (B)</p> Signup and view all the answers

Which term describes the process of using DNA as a template to synthesize RNA?

<p>Transcription (A)</p> Signup and view all the answers

What is the role of DNA ligase in DNA replication?

<p>Join Okazaki fragments (B)</p> Signup and view all the answers

Which of the following is involved in oxidative phosphorylation?

<p>Electron transport chain (D)</p> Signup and view all the answers

What is the primary outcome of Meiosis II?

<p>Four genetically distinct haploid daughter cells (C)</p> Signup and view all the answers

Which statement about enzymes is true?

<p>Enzymes are biological catalysts that speed up reactions. (B)</p> Signup and view all the answers

What happens to some energy during energy transfer according to the second law of thermodynamics?

<p>It becomes unusable, often as heat. (D)</p> Signup and view all the answers

Which molecule is known as the primary energy carrier in cells?

<p>ATP (D)</p> Signup and view all the answers

How does ATP release energy?

<p>By hydrolyzing a phosphate bond resulting in ADP and Pi. (C)</p> Signup and view all the answers

What defines the Law of Segregation?

<p>Alleles segregate during the formation of gametes. (A)</p> Signup and view all the answers

Which of the following is a characteristic of polygenic inheritance?

<p>Traits are influenced by multiple genes. (C)</p> Signup and view all the answers

In complete dominance, how is a recessive allele expressed?

<p>Only when two copies are present. (C)</p> Signup and view all the answers

What is the role of mitochondria in genetic inheritance?

<p>They contain their own DNA, inherited maternally. (B)</p> Signup and view all the answers

What is the genotype represented by XᴬXᵃ?

<p>Heterozygous (A)</p> Signup and view all the answers

What does aneuploidy refer to in genetics?

<p>An abnormal number of chromosomes. (A)</p> Signup and view all the answers

Which best describes a dominant allele?

<p>Can mask the presence of a recessive allele. (A)</p> Signup and view all the answers

What does the term phenotype refer to?

<p>The observable traits of an organism. (D)</p> Signup and view all the answers

Which process synthesizes proteins from mRNA?

<p>Translation (A)</p> Signup and view all the answers

What is the main function of NADH and FADH₂ in cellular respiration?

<p>They carry energy in the form of electrons and protons. (C)</p> Signup and view all the answers

Which type of RNA is responsible for bringing amino acids to the ribosome?

<p>tRNA (A)</p> Signup and view all the answers

What is the function of DNA ligase during DNA replication?

<p>Joins Okazaki fragments together. (C)</p> Signup and view all the answers

What process generates ATP during photosynthesis?

<p>Light-dependent reactions (D)</p> Signup and view all the answers

What distinguishes homologous chromosomes?

<p>They pair during meiosis and have different alleles. (D)</p> Signup and view all the answers

Which genetic repair pathway fixes small DNA base alterations?

<p>Base excision repair (BER) (D)</p> Signup and view all the answers

Which of the following statements best describes codominance?

<p>Both alleles are expressed equally in the phenotype. (C)</p> Signup and view all the answers

What occurs during recombination in meiosis?

<p>Exchange of genetic material between homologous chromosomes. (D)</p> Signup and view all the answers

Which technique is used to amplify specific DNA sequences?

<p>Polymerase Chain Reaction (PCR) (A)</p> Signup and view all the answers

What is the primary role of RNA polymerase during transcription?

<p>Copy DNA into messenger RNA (mRNA). (D)</p> Signup and view all the answers

At what stage in DNA replication does the DNA polymerase add new nucleotides?

<p>Elongation (B)</p> Signup and view all the answers

Which of the following types of RNA is a structural component of ribosomes?

<p>rRNA (D)</p> Signup and view all the answers

How do epigenetic modifications affect gene expression?

<p>By modifying the interaction of DNA with proteins. (A)</p> Signup and view all the answers

What describes the nature of DNA replication?

<p>Semiconservative, with one old and one new strand. (C)</p> Signup and view all the answers

Flashcards

Cytoplasm

The gel-like substance filling the cell between the membrane and nucleus; contains organelles and cytosol.

Ribosomes

Cell structures responsible for protein synthesis, found free in the cytosol or attached to the ER.

Endomembrane System

A network of membranes (nuclear envelope, ER, Golgi, lysosomes, vacuoles, plasma membrane) that work together to modify, sort, and transport proteins.

Mitochondria

Organelles that perform cellular respiration, producing energy (ATP).

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Cytoskeleton

A network of protein fibers that provides cell structure, movement, and organization.

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Nucleus (Eukaryotic)

The cell's control center, containing DNA and directing cellular activities.

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Plasma Membrane

The outer boundary of the cell composed of a phospholipid bilayer, regulating what enters and leaves.

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Chromosomes

Structures that contain genetic information (DNA) in the nucleus.

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Microtubules

Protein filaments that help with cell division and movement.

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Centrosomes and Centrioles

Organelles that organize microtubules and aid in cell division.

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Microfilaments

Actin filaments involved in muscle movement, cell division, and cell shape change.

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Animal Extracellular Matrix (ECM)

Support structure outside the animal cell, offering both structural and biochemical support.

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Intercellular Junctions

Structures facilitating cell-to-cell contact and communication.

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Tight Junctions

Animal cell junctions preventing fluid leakage between cells.

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Desmosomes

Animal cell junctions that anchor cells together, providing strength.

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Gap Junctions

Animal cell junctions providing channels for communication.

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Phospholipids

Key components of cell membranes, with hydrophilic heads and hydrophobic tails.

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Passive Transport

Movement of substances across the membrane without energy input.

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Active Transport

Movement of substances against their concentration gradient.

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Meiosis

Cell division producing four genetically unique haploid cells.

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Binary Fission

A simple process where a single-celled prokaryote splits into two identical cells.

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Cell Cycle Phases

The distinct stages of eukaryotic cell division: G1 (growth), S (DNA replication), G2 (preparation), and M (mitosis/meiosis).

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Circular vs. Linear Chromosomes

Prokaryotes have single, circular DNA molecules. Eukaryotes have linear chromosomes, with multiple molecules.

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Spindle Apparatus

Microtubule structure formed during cell division, pulling replicated chromosomes apart.

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Centrosome

Structure in eukaryotic cells that organizes microtubules for spindle formation and cell division.

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Kinetochore

Protein complex on chromosomes that connects to spindle fibers for chromosome movement during mitosis.

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Cleavage Furrow

Indentation of the cell membrane in animal cells that pinches in, dividing the cytoplasm during cytokinesis.

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Cell Plate

Structure formed in plant cells during cytokinesis, separating daughter cells and forming a new cell wall.

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Prophase

The first stage of mitosis, where chromosomes condense and the spindle apparatus starts forming.

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Prometaphase

The stage where the nuclear envelope breaks down and spindle fibers attach to chromosomes.

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Metaphase

The stage where chromosomes align at the center of the cell, forming the metaphase plate.

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Anaphase

The stage where sister chromatids separate and are pulled to opposite poles of the cell.

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Telophase

The final stage of mitosis, where chromosomes decondense, the nuclear envelope reforms around each set, and the cell prepares for cytokinesis.

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Kinetic Energy

Energy associated with motion, like molecules moving or water flowing.

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Potential Energy

Stored energy, like chemical bonds in molecules or gravitational potential.

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Peptidyl Transferase

An enzyme in the large ribosomal subunit that forms peptide bonds between amino acids during protein synthesis.

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Stop Codon

Three-base sequences (UAA, UGA, or UAG) on mRNA that signal the end of protein synthesis.

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Release Factor

A protein that binds to a stop codon in the A site of the ribosome, triggering the release of the completed polypeptide chain.

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Aerobic Respiration

Cellular respiration that uses oxygen to break down glucose and produce ATP.

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Anaerobic Respiration

Cellular respiration that occurs without oxygen, producing less ATP than aerobic respiration.

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Mitochondrial Cristae

Highly folded inner membrane of mitochondria, increasing surface area for ATP production during cellular respiration.

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Stroma

The fluid-filled space within a chloroplast, containing enzymes for the Calvin cycle.

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Thylakoid Lumen

The space inside a thylakoid, where protons accumulate during photophosphorylation.

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Photosystem

Clusters of pigments and proteins in the thylakoid membrane that capture light energy and transfer it to electrons.

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Excited Electron

An electron that has absorbed energy from light and moved to a higher energy level.

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Chemiosmosis (in Chloroplasts)

The process of ATP production in chloroplasts, driven by the movement of protons across the thylakoid membrane.

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Calvin Cycle

A metabolic pathway in the stroma of chloroplasts that uses ATP and NADPH from the light reactions to fix carbon dioxide into organic molecules.

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Light Reactions

The first stage of photosynthesis, occurring in the thylakoid membranes, converting light energy into chemical energy (ATP and NADPH).

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Photophosphorylation

The process of ATP production during light reactions in photosynthesis, involving electron transport and proton gradient.

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Oxidative Phosphorylation

The process of ATP production in mitochondria, involving electron transport and proton gradient.

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Haploid Cell

A cell containing only one set of chromosomes (n), found in gametes (sperm and egg cells).

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Gametes

Sex cells (sperm and egg cells) that fuse during fertilization to form a zygote.

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Enzymes

Biological catalysts that speed up chemical reactions without being consumed in the process.

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Chemical Energy

A form of potential energy stored in the bonds of molecules.

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ATP

The primary energy carrier in cells, used to power various cellular processes.

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What does ATP hydrolysis release?

ATP hydrolysis releases energy, ADP, and inorganic phosphate (Pi).

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First Law of Thermodynamics

Energy cannot be created or destroyed, only transformed from one form to another.

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Second Law of Thermodynamics

In any energy transfer, some energy becomes unusable, increasing entropy (disorder) in the system.

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Law of Segregation

Each individual has two alleles for each trait that separate during gamete formation, with each gamete receiving only one allele per gene.

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Law of Independent Assortment

The inheritance of one trait is independent of the inheritance of another trait, assuming genes are on different chromosomes or far apart.

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Dominant Allele

An allele whose effect is expressed even when only one copy is present (heterozygous).

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Recessive Allele

An allele whose effect is only expressed when two copies are present (homozygous).

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Genotype vs. Phenotype

Genotype is the genetic makeup of an organism, while phenotype is the observable traits resulting from the genotype and environment.

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Golgi Apparatus

A series of flattened, membrane-bound sacs (cisternae) that modify, sort, package, and ship proteins and lipids from the ER to their final destinations.

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Peroxisomes

Small, membrane-bound organelles containing enzymes for breaking down fatty acids and detoxifying harmful substances.

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Intermediate Filaments

Provide mechanical strength and stability to the cell, helping maintain its shape and providing support.

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Vacuoles (Plant)

Large, membrane-bound sacs in plants, storing water, nutrients, and waste products, and maintaining turgor pressure.

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Vesicles

Small, membrane-bound sacs involved in transporting and storing materials like proteins, lipids, and waste products within the cell.

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Centrioles

Structures made of microtubules in animal cells involved in organizing spindle fibers during cell division.

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Chloroplasts

Membrane-bound organelles containing chlorophyll, responsible for photosynthesis in plants.

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Cell Wall

A rigid outer layer made of cellulose (plants), chitin (fungi), or peptidoglycan (bacteria), providing support, protection, and shape maintenance.

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G₁ Checkpoint

Assesses whether the cell has sufficient resources to divide and checks DNA integrity before replication.

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G₂ Checkpoint

Verifies that DNA replication has been completed accurately and the cell is ready for mitosis.

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Metaphase Checkpoint

Ensures that chromosomes are correctly aligned and attached to spindle fibers before proceeding to anaphase.

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Polygenic Inheritance

Traits controlled by many genes, resulting in a range of phenotypes, like height or skin color.

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Mitochondrial Inheritance

Inheritance of genes from mitochondria, which have their own DNA. These genes are only passed down from the mother.

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Aneuploidy

An abnormal number of chromosomes, often resulting in genetic disorders.

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Down Syndrome

A genetic disorder caused by an extra copy of chromosome 21 (trisomy 21).

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Genetic Mapping

The process of locating genes on chromosomes, helping diagnose genetic conditions.

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Molecular Biology

The study of the structure, function, and regulation of biological molecules, like DNA, RNA, and proteins.

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Gene

A segment of DNA that contains instructions for building proteins or RNA molecules.

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Genome

The complete set of genetic material in an organism.

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Genomics

The study of the structure, function, and evolution of genomes.

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mRNA

Messenger RNA, carries genetic information from DNA to the ribosome for protein synthesis.

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Genetic Code

The set of rules that translates the mRNA sequence into an amino acid sequence.

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DNA Replication

The process of copying DNA, ensuring each new cell receives a complete set of genetic instructions.

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Gene Expression

The process of using the genetic code in DNA to synthesize proteins or RNA molecules.

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Epigenetic Modifications

Changes in gene expression without alterations to the DNA sequence, influenced by environmental factors.

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Transcription Factors

Proteins that help RNA polymerase bind to the DNA and initiate transcription.

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RNA Polymerase

An enzyme that synthesizes RNA using a DNA template.

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Introns

Non-coding sequences within a gene that are removed during RNA processing.

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Exons

Coding sequences within a gene that are spliced together to form mature mRNA.

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Translation

The process of converting mRNA into a protein.

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ATP (Adenosine Triphosphate)

The primary energy currency of the cell.

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Anabolic Reactions

Chemical reactions that build molecules, requiring energy.

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Catabolic Reactions

Chemical reactions that break down molecules, releasing energy.

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Glycolysis

The breakdown of glucose into pyruvate, producing a small amount of ATP.

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Study Notes

Cytoplasm

  • A gel-like substance filling the space between the plasma membrane and the nucleus.
  • Contains cytosol (the fluid portion).
  • Site of protein synthesis and metabolism.
  • Holds organelles in place, providing support and structure.
  • Organelles for energy, synthesis, storage, and transport are within.

Ribosomes

  • Can be free or attached to membranes.
  • Involved in protein synthesis.
  • Made of ribosomal RNA (rRNA) and proteins.

Endomembrane System

  • Coordinates protein synthesis and transport.
  • Includes nuclear envelope, endoplasmic reticulum (ER), Golgi apparatus, lysosomes, and plasma membrane.

Mitochondria

  • Performs cellular respiration.
  • Produces ATP (adenosine triphosphate).
  • Has an outer and inner membrane, cristae increasing the surface area.

Cytoskeleton

  • Provides structural support, aids in movement, and organizes the cell.
  • A network of fibers, including microtubules, microfilaments, and intermediate filaments.

Animal Cells

  • Contain lysosomes, centrioles, and flagella.

Plant Cells

  • Contain chloroplasts, central vacuole, tonoplast, and cell wall.

Nucleus in Eukaryotic Cells

  • Contains most of the cell's DNA.
  • Surrounded by the nuclear envelope, with pores regulating molecule passage.
  • Nucleolus assembles ribosomes.
  • Chromatin forms chromosomes.

Common Cell Elements

Plasma Membrane

  • Surrounds the cell, regulating substance flow.
  • Composed of a phospholipid bilayer.

Chromosomes

  • Contain genetic material.

Cytoskeleton (details)

  • Provides structural support and aids in cell movement.

Endomembrane System (details)

  • Includes nuclear envelope, ER (smooth and rough), Golgi apparatus, lysosomes, vacuoles, and plasma membrane.
  • Coordinates protein synthesis, modification, and transport.

Endoplasmic Reticulum (ER)

Smooth ER

  • Synthesizes lipids and regulates carbohydrate metabolism.

Rough ER

  • Involved in protein modification and folding, studded with ribosomes.

Golgi Apparatus

  • Flattened membranous sacs (cisternae).
  • Modifies ER products, synthesizes macromolecules, and sorts/packages proteins.

Chloroplasts

  • Found in plant cells, conduct photosynthesis.

Peroxisomes

  • Involved in lipid metabolism and detoxification.

Extracellular Structures

Plant Cell Walls

  • Provide structural support.

Animal Extracellular Matrix (ECM)

  • Provides structural and biochemical support to surrounding cells.

Intercellular Junctions

  • Facilitate cell communication and physical contact.

Types (animal/plant)

  • Tight junctions, gap junctions, desmosomes in animal cells; plasmodesmata in plant cells.

Plant Plasmodesmata

  • Membrane-lined pores that allow communication and transport of small molecules. (ions, hormones, proteins)

Animal Cell Junctions

  • Tight junctions, desmosomes, and gap junctions.

Plasma Membrane (Details)

  • Separates cells from surroundings; selectively permeable.
  • Composed of a phospholipid bilayer with embedded proteins.

Membrane Proteins

  • Integral (spanning membrane) and peripheral (attached to surface) proteins.

Membrane Fluidity

  • Cholesterol maintains membrane stability; varies with temperature.

Passive Transport

  • Movement of substances down a concentration gradient without energy input.
  • Types: simple diffusion, facilitated diffusion, osmosis

Active Transport

  • Movement of substances against concentration gradients, requiring energy.
  • Types: primary (direct) and secondary (indirect) active transport
  • Example: Sodium-potassium pump.

Bulk Transport

  • Movement of large materials.
  • Exocytosis (materials out) and endocytosis (materials in). (types: phagocytosis, pinocytosis, receptor-mediated endocytosis)

Cell: The Smallest Functional Unit of Life

Nucleic Acids

  • Carry genetic information (DNA/RNA).

Cell Membrane

  • Regulates the internal environment of the cell.

Cell Wall (Plants)

  • Provides protection and support.

Gene

  • A segment of DNA that serves as a blueprint for biological traits.

Organelles

  • Carry out ATP production, protein synthesis, and cellular structure maintenance. (cytosol, cytoplasm, cytoskeleton, ATP-producing structures)

Cell Cycle

  • A series of events leading to cell division: Growth, DNA replication, chromosome distribution.

G1, S, G2, M Phases

  • Key phases of the eukaryotic cell cycle.

Prokaryotic Cells

  • Use binary fission.

Eukaryotic Cells

  • Have complex cycles with distinct phases.

Spindle Apparatus

  • Made of microtubules; separates chromatids during cell division.

Centrosomes and Centrioles

  • Organize microtubules.

Kinetochore

  • Protein complexes that attach chromosomes to spindle fibers.

Cleavage Furrow/ Cell Plate

  • Structures formed during cytokinesis to divide cytoplasm (animal/plant).

Stages of Mitosis

  • Prophase, Prometaphase, Metaphase, Anaphase, Telophase

Organisms

  • Organisms have complex organization.
  • Respond to environmental stimuli.
  • Grow and develop.
  • Maintain internal balance (homeostasis).

Bioenergetics

  • Study of energy transformations.

Laws of Thermodynamics

  • First Law: Energy conservation.
  • Second Law: Entropy increases.

Role of Enzymes

  • Lower activation energy; bind to substrates.

ATP (Adenosine Triphosphate)

  • Primary energy carrier in cells.
  • Generated through substrate-level phosphorylation and chemiosmosis.
  • Releases energy via hydrolysis.

Chemiosmosis

  • ATP synthesis through electron transport.

Redox Reactions

  • Key to ATP production in metabolic pathways.

Catalysts

  • Enzymes speed up reactions.

DNA Replication

  • DNA molecule is copied to produce two identical molecules.
  • Semiconservative Replication.

DNA Replication (details)

  • Enzymes involved: DNA helicase, DNA polymerase, DNA ligase.
  • Replication fork.

Transcription

  • DNA is used as a template to produce mRNA.

Translation

  • mRNA is translated into protein at ribosomes.

Central Dogma

  • Information flows from genotype (DNA) to phenotype (protein) through RNA.

Translation (details)

  • Ribosomes, tRNA, mRNA, codons, anticodons.

Elongation (Translation)

  • Ribosome reads mRNA (codon-by-codon), forms peptide bonds.

Termination (Translation)

  • Stop codon signals; polypeptide released; ribosome dissociates.

Cellular Respiration

  • Breakdown of glucose to generate ATP in mitochondria.
  • Aerobic and anaerobic respiration. (glycolysis, citric acid cycle, electron transport chain).

Photosynthesis

  • Light-dependent and light-independent reactions. (light reactions, Calvin cycle).

Chloroplast Structure

  • Thylakoids, grana, stroma.

Carbon Fixation (Calvin Cycle)

  • Location, process of fixing carbon dioxide.

Products of Photosynthesis

  • ATP and NADPH (light reactions), and oxygen (released as a byproduct)
  • Sugars, starch, or other organic compounds (formed in the Calvin cycle)

Meiosis

  • Cell division reducing chromosome number; occurs in sexually reproducing organisms.
  • Resulting in genetically distinct haploid cells (gametes).
  • Stages: Meiosis I and Meiosis II.
  • Events like crossing over and independent assortment.

Meiosis I (details)

  • Prophase I, Metaphase I, Anaphase I, Telophase I

Meiosis II (details)

  • Prophase II, Metaphase II, Anaphase II, Telophase II

Gametes

  • Reproductive cells (sperm and egg) with half the genetic material (haploid).

Zygote

  • Fertilized egg; restores diploid number.

Bivalent/Tetrad

  • Definition and significance during meiosis.

Crossing Over

Haploid/Diploid

  • Definitions and significance.

Genetic Variation

  • Sources of variation in meiosis

Independent Assortment

  • Basis for genetic diversity.

Chiasmata, Synapsis, Synaptonemal Complex

  • Definitions.

Reduction Division (Meiosis)

  • Significance.

Genetic Recombination

  • Importance in evolution.

Mitosis Outcomes

  • Two genetically identical diploid daughter cells.
  • Importance in growth and repair.

G1, G2, and Metaphase Checkpoints

  • Function & importance.

Growth and Development

  • Importance of mitosis.

Prophase, Prometaphase, Metaphase, Anaphase, and Telophase (Mitosis)

  • Stages of mitosis

Prokaryotic Chromosomes

  • Circular chromosomes

Cytokinesis (Mitosis/Meiosis)

  • Cytoplasm division.

Mendelian Genetics

  • Laws of Segregation and Independent Assortment.

Dominant/Recessive Alleles

  • Definitions of dominance and recessiveness.

Genotype/Phenotype

  • Definitions of each.

Chromosomal Genetics

  • Study of genes on chromosomes.

Homologous Chromosomes

  • Definition of homologous pair.

Sex Chromosomes, Sex-Linked Traits

  • Example of sex-linked trait and inheritance patterns.

Incomplete Dominance/Codominance/Polygenic Inheritance

  • Definitions of each.

Mitochondrial Inheritance

Chromosomal Disorders

Molecular Biology Techniques

  • PCR, Gel Electrophoresis, RNA-seq, CRISPR-Cas9.

DNA Replication (detailed stages)

  • Origins of replication.
  • Leading and lagging strands.
  • Okazaki fragments.
  • Proofreading and repair mechanisms.

Transcription

  • Initiation, elongation and termination

RNA processing

  • Types of modifications. (5' Capping, Polyadenylation, splicing)

Gene Regulation (transcriptional)

  • Regulation through transcription factors and epigenetic modifications.

Post-translational Modifications

  • Further modification of protein chains.

Epigenetic Modifications

  • How gene expression can be altered without changing the DNA sequence.

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