Podcast
Questions and Answers
What is the primary function of ribosomes?
What is the primary function of ribosomes?
- Storage of genetic material
- Energy production
- Cell replication
- Protein synthesis (correct)
Which structure is responsible for modifying, sorting, and packaging proteins?
Which structure is responsible for modifying, sorting, and packaging proteins?
- Mitochondria
- Endoplasmic Reticulum
- Golgi Apparatus (correct)
- Nucleus
What is one key difference between the rough ER and the smooth ER?
What is one key difference between the rough ER and the smooth ER?
- Rough ER synthesizes carbohydrates while smooth ER does not
- Rough ER is involved in detoxification while smooth ER is not
- Smooth ER is involved in protein folding while rough ER is not
- Rough ER contains ribosomes while smooth ER does not (correct)
Which structure in eukaryotic cells is primarily involved in assembling ribosomes?
Which structure in eukaryotic cells is primarily involved in assembling ribosomes?
What are chloroplasts primarily responsible for in plant cells?
What are chloroplasts primarily responsible for in plant cells?
Which organelle is crucial for the production of ATP?
Which organelle is crucial for the production of ATP?
What role does the cytoskeleton play in a cell?
What role does the cytoskeleton play in a cell?
What is the main function of lysosomes in animal cells?
What is the main function of lysosomes in animal cells?
What is the primary function of microtubules in a cell?
What is the primary function of microtubules in a cell?
Which type of intercellular junction prevents fluid leakage between cells?
Which type of intercellular junction prevents fluid leakage between cells?
Which transport mechanism requires energy to move substances against a concentration gradient?
Which transport mechanism requires energy to move substances against a concentration gradient?
What is a characteristic of meiosis that differentiates it from mitosis?
What is a characteristic of meiosis that differentiates it from mitosis?
In which phase of meiosis do homologous chromosomes separate?
In which phase of meiosis do homologous chromosomes separate?
What role does cholesterol play in the plasma membrane?
What role does cholesterol play in the plasma membrane?
What structure forms during Prophase I of meiosis allowing genetic recombination?
What structure forms during Prophase I of meiosis allowing genetic recombination?
Which of the following is a characteristic of the extracellular matrix (ECM) in animal cells?
Which of the following is a characteristic of the extracellular matrix (ECM) in animal cells?
Which of the following is NOT a type of passive transport?
Which of the following is NOT a type of passive transport?
What is the role of the gene in a cell?
What is the role of the gene in a cell?
What happens during crossing over in meiosis?
What happens during crossing over in meiosis?
What defines a cell as haploid?
What defines a cell as haploid?
What occurs during Telophase II of meiosis?
What occurs during Telophase II of meiosis?
What is the main function of the Golgi apparatus?
What is the main function of the Golgi apparatus?
Which structure contains digestive enzymes that help break down waste materials?
Which structure contains digestive enzymes that help break down waste materials?
What role do peroxisomes play in the cell?
What role do peroxisomes play in the cell?
Which component of the cytoskeleton provides mechanical strength and stability to the cell?
Which component of the cytoskeleton provides mechanical strength and stability to the cell?
What is the purpose of vacuoles in plant cells?
What is the purpose of vacuoles in plant cells?
How does mitosis ensure genetic consistency in daughter cells?
How does mitosis ensure genetic consistency in daughter cells?
What occurs during the G₁ checkpoint of the cell cycle?
What occurs during the G₁ checkpoint of the cell cycle?
What distinguishes Meiosis II from mitosis?
What distinguishes Meiosis II from mitosis?
Which organelle is involved in photosynthesis?
Which organelle is involved in photosynthesis?
What forms during cytokinesis in plant cells?
What forms during cytokinesis in plant cells?
During which phase do sister chromatids separate?
During which phase do sister chromatids separate?
What is the role of spindle fibers in cell division?
What is the role of spindle fibers in cell division?
What structure is primarily responsible for transporting materials within the cell?
What structure is primarily responsible for transporting materials within the cell?
What is the end product of mitosis in terms of chromosome number?
What is the end product of mitosis in terms of chromosome number?
Which process occurs in the mitochondrion to generate ATP?
Which process occurs in the mitochondrion to generate ATP?
What is the main role of the thylakoid membranes in chloroplasts?
What is the main role of the thylakoid membranes in chloroplasts?
Which of the following is not a product of the light reactions in photosynthesis?
Which of the following is not a product of the light reactions in photosynthesis?
What is the function of the ribosome in a cell?
What is the function of the ribosome in a cell?
In which cellular compartment does the Calvin cycle take place?
In which cellular compartment does the Calvin cycle take place?
What is the final electron acceptor in oxidative phosphorylation?
What is the final electron acceptor in oxidative phosphorylation?
Which component increases the surface area of the inner mitochondrial membrane?
Which component increases the surface area of the inner mitochondrial membrane?
What is the primary purpose of chemiosmosis during photosynthesis?
What is the primary purpose of chemiosmosis during photosynthesis?
Which of the following correctly describes the structure of the plasma membrane?
Which of the following correctly describes the structure of the plasma membrane?
How does the arrangement of chlorophyll in plants affect photosynthesis?
How does the arrangement of chlorophyll in plants affect photosynthesis?
What role do the ribosomal subunits play during protein synthesis?
What role do the ribosomal subunits play during protein synthesis?
Which function is not performed by the smooth endoplasmic reticulum (SER)?
Which function is not performed by the smooth endoplasmic reticulum (SER)?
What mechanism leads to the production of NADPH during photosynthesis?
What mechanism leads to the production of NADPH during photosynthesis?
What role do transcription factors play in eukaryotic gene expression?
What role do transcription factors play in eukaryotic gene expression?
What role does the nucleolus play within the nucleus?
What role does the nucleolus play within the nucleus?
During RNA processing, which of the following modifications occurs?
During RNA processing, which of the following modifications occurs?
What initiates translation at the ribosome?
What initiates translation at the ribosome?
Which of the following correctly describes the process of glycolysis?
Which of the following correctly describes the process of glycolysis?
What is the primary function of the citric acid cycle?
What is the primary function of the citric acid cycle?
How does energy coupling work in cellular processes?
How does energy coupling work in cellular processes?
What is the role of chlorophyll in photosynthesis?
What is the role of chlorophyll in photosynthesis?
Which of the following is NOT a method of regulating gene expression?
Which of the following is NOT a method of regulating gene expression?
What characterizes cancer cells in terms of metabolic processes?
What characterizes cancer cells in terms of metabolic processes?
What is the function of introns in eukaryotic mRNA?
What is the function of introns in eukaryotic mRNA?
What is the primary method of reproduction in prokaryotic cells?
What is the primary method of reproduction in prokaryotic cells?
Which component is crucial for spindle formation during mitosis?
Which component is crucial for spindle formation during mitosis?
During which phase of mitosis do chromosomes align at the metaphase plate?
During which phase of mitosis do chromosomes align at the metaphase plate?
Which process releases energy by breaking ATP bonds?
Which process releases energy by breaking ATP bonds?
What type of energy is associated with the motion of molecules?
What type of energy is associated with the motion of molecules?
Which law states that energy cannot be created or destroyed, only transformed?
Which law states that energy cannot be created or destroyed, only transformed?
Which enzyme is responsible for adding free nucleotides during DNA replication?
Which enzyme is responsible for adding free nucleotides during DNA replication?
What is produced when mRNA is translated at the ribosome?
What is produced when mRNA is translated at the ribosome?
Which structure attaches chromosomes to spindle fibers during cell division?
Which structure attaches chromosomes to spindle fibers during cell division?
During which process does DNA unwind and break hydrogen bonds between base pairs?
During which process does DNA unwind and break hydrogen bonds between base pairs?
In which part of eukaryotic cells does transcription occur?
In which part of eukaryotic cells does transcription occur?
Which term describes the process of using DNA as a template to synthesize RNA?
Which term describes the process of using DNA as a template to synthesize RNA?
What is the role of DNA ligase in DNA replication?
What is the role of DNA ligase in DNA replication?
Which of the following is involved in oxidative phosphorylation?
Which of the following is involved in oxidative phosphorylation?
What is the primary outcome of Meiosis II?
What is the primary outcome of Meiosis II?
Which statement about enzymes is true?
Which statement about enzymes is true?
What happens to some energy during energy transfer according to the second law of thermodynamics?
What happens to some energy during energy transfer according to the second law of thermodynamics?
Which molecule is known as the primary energy carrier in cells?
Which molecule is known as the primary energy carrier in cells?
How does ATP release energy?
How does ATP release energy?
What defines the Law of Segregation?
What defines the Law of Segregation?
Which of the following is a characteristic of polygenic inheritance?
Which of the following is a characteristic of polygenic inheritance?
In complete dominance, how is a recessive allele expressed?
In complete dominance, how is a recessive allele expressed?
What is the role of mitochondria in genetic inheritance?
What is the role of mitochondria in genetic inheritance?
What is the genotype represented by XᴬXᵃ?
What is the genotype represented by XᴬXᵃ?
What does aneuploidy refer to in genetics?
What does aneuploidy refer to in genetics?
Which best describes a dominant allele?
Which best describes a dominant allele?
What does the term phenotype refer to?
What does the term phenotype refer to?
Which process synthesizes proteins from mRNA?
Which process synthesizes proteins from mRNA?
What is the main function of NADH and FADH₂ in cellular respiration?
What is the main function of NADH and FADH₂ in cellular respiration?
Which type of RNA is responsible for bringing amino acids to the ribosome?
Which type of RNA is responsible for bringing amino acids to the ribosome?
What is the function of DNA ligase during DNA replication?
What is the function of DNA ligase during DNA replication?
What process generates ATP during photosynthesis?
What process generates ATP during photosynthesis?
What distinguishes homologous chromosomes?
What distinguishes homologous chromosomes?
Which genetic repair pathway fixes small DNA base alterations?
Which genetic repair pathway fixes small DNA base alterations?
Which of the following statements best describes codominance?
Which of the following statements best describes codominance?
What occurs during recombination in meiosis?
What occurs during recombination in meiosis?
Which technique is used to amplify specific DNA sequences?
Which technique is used to amplify specific DNA sequences?
What is the primary role of RNA polymerase during transcription?
What is the primary role of RNA polymerase during transcription?
At what stage in DNA replication does the DNA polymerase add new nucleotides?
At what stage in DNA replication does the DNA polymerase add new nucleotides?
Which of the following types of RNA is a structural component of ribosomes?
Which of the following types of RNA is a structural component of ribosomes?
How do epigenetic modifications affect gene expression?
How do epigenetic modifications affect gene expression?
What describes the nature of DNA replication?
What describes the nature of DNA replication?
Flashcards
Cytoplasm
Cytoplasm
The gel-like substance filling the cell between the membrane and nucleus; contains organelles and cytosol.
Ribosomes
Ribosomes
Cell structures responsible for protein synthesis, found free in the cytosol or attached to the ER.
Endomembrane System
Endomembrane System
A network of membranes (nuclear envelope, ER, Golgi, lysosomes, vacuoles, plasma membrane) that work together to modify, sort, and transport proteins.
Mitochondria
Mitochondria
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Cytoskeleton
Cytoskeleton
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Nucleus (Eukaryotic)
Nucleus (Eukaryotic)
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Plasma Membrane
Plasma Membrane
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Chromosomes
Chromosomes
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Microtubules
Microtubules
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Centrosomes and Centrioles
Centrosomes and Centrioles
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Microfilaments
Microfilaments
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Animal Extracellular Matrix (ECM)
Animal Extracellular Matrix (ECM)
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Intercellular Junctions
Intercellular Junctions
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Tight Junctions
Tight Junctions
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Desmosomes
Desmosomes
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Gap Junctions
Gap Junctions
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Phospholipids
Phospholipids
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Passive Transport
Passive Transport
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Active Transport
Active Transport
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Meiosis
Meiosis
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Binary Fission
Binary Fission
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Cell Cycle Phases
Cell Cycle Phases
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Circular vs. Linear Chromosomes
Circular vs. Linear Chromosomes
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Spindle Apparatus
Spindle Apparatus
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Centrosome
Centrosome
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Kinetochore
Kinetochore
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Cleavage Furrow
Cleavage Furrow
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Cell Plate
Cell Plate
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Prophase
Prophase
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Prometaphase
Prometaphase
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Metaphase
Metaphase
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Anaphase
Anaphase
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Telophase
Telophase
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Kinetic Energy
Kinetic Energy
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Potential Energy
Potential Energy
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Peptidyl Transferase
Peptidyl Transferase
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Stop Codon
Stop Codon
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Release Factor
Release Factor
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Aerobic Respiration
Aerobic Respiration
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Anaerobic Respiration
Anaerobic Respiration
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Mitochondrial Cristae
Mitochondrial Cristae
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Stroma
Stroma
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Thylakoid Lumen
Thylakoid Lumen
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Photosystem
Photosystem
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Excited Electron
Excited Electron
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Chemiosmosis (in Chloroplasts)
Chemiosmosis (in Chloroplasts)
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Calvin Cycle
Calvin Cycle
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Light Reactions
Light Reactions
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Photophosphorylation
Photophosphorylation
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Oxidative Phosphorylation
Oxidative Phosphorylation
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Haploid Cell
Haploid Cell
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Gametes
Gametes
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Enzymes
Enzymes
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Chemical Energy
Chemical Energy
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ATP
ATP
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What does ATP hydrolysis release?
What does ATP hydrolysis release?
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First Law of Thermodynamics
First Law of Thermodynamics
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Second Law of Thermodynamics
Second Law of Thermodynamics
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Law of Segregation
Law of Segregation
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Law of Independent Assortment
Law of Independent Assortment
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Dominant Allele
Dominant Allele
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Recessive Allele
Recessive Allele
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Genotype vs. Phenotype
Genotype vs. Phenotype
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Golgi Apparatus
Golgi Apparatus
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Peroxisomes
Peroxisomes
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Intermediate Filaments
Intermediate Filaments
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Vacuoles (Plant)
Vacuoles (Plant)
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Vesicles
Vesicles
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Centrioles
Centrioles
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Chloroplasts
Chloroplasts
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Cell Wall
Cell Wall
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G₁ Checkpoint
G₁ Checkpoint
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G₂ Checkpoint
G₂ Checkpoint
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Metaphase Checkpoint
Metaphase Checkpoint
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Polygenic Inheritance
Polygenic Inheritance
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Mitochondrial Inheritance
Mitochondrial Inheritance
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Aneuploidy
Aneuploidy
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Down Syndrome
Down Syndrome
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Genetic Mapping
Genetic Mapping
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Molecular Biology
Molecular Biology
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Gene
Gene
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Genome
Genome
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Genomics
Genomics
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mRNA
mRNA
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Genetic Code
Genetic Code
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DNA Replication
DNA Replication
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Gene Expression
Gene Expression
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Epigenetic Modifications
Epigenetic Modifications
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Transcription Factors
Transcription Factors
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RNA Polymerase
RNA Polymerase
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Introns
Introns
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Exons
Exons
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Translation
Translation
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ATP (Adenosine Triphosphate)
ATP (Adenosine Triphosphate)
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Anabolic Reactions
Anabolic Reactions
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Catabolic Reactions
Catabolic Reactions
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Glycolysis
Glycolysis
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Study Notes
Cytoplasm
- A gel-like substance filling the space between the plasma membrane and the nucleus.
- Contains cytosol (the fluid portion).
- Site of protein synthesis and metabolism.
- Holds organelles in place, providing support and structure.
- Organelles for energy, synthesis, storage, and transport are within.
Ribosomes
- Can be free or attached to membranes.
- Involved in protein synthesis.
- Made of ribosomal RNA (rRNA) and proteins.
Endomembrane System
- Coordinates protein synthesis and transport.
- Includes nuclear envelope, endoplasmic reticulum (ER), Golgi apparatus, lysosomes, and plasma membrane.
Mitochondria
- Performs cellular respiration.
- Produces ATP (adenosine triphosphate).
- Has an outer and inner membrane, cristae increasing the surface area.
Cytoskeleton
- Provides structural support, aids in movement, and organizes the cell.
- A network of fibers, including microtubules, microfilaments, and intermediate filaments.
Animal Cells
- Contain lysosomes, centrioles, and flagella.
Plant Cells
- Contain chloroplasts, central vacuole, tonoplast, and cell wall.
Nucleus in Eukaryotic Cells
- Contains most of the cell's DNA.
- Surrounded by the nuclear envelope, with pores regulating molecule passage.
- Nucleolus assembles ribosomes.
- Chromatin forms chromosomes.
Common Cell Elements
Plasma Membrane
- Surrounds the cell, regulating substance flow.
- Composed of a phospholipid bilayer.
Chromosomes
- Contain genetic material.
Cytoskeleton (details)
- Provides structural support and aids in cell movement.
Endomembrane System (details)
- Includes nuclear envelope, ER (smooth and rough), Golgi apparatus, lysosomes, vacuoles, and plasma membrane.
- Coordinates protein synthesis, modification, and transport.
Endoplasmic Reticulum (ER)
Smooth ER
- Synthesizes lipids and regulates carbohydrate metabolism.
Rough ER
- Involved in protein modification and folding, studded with ribosomes.
Golgi Apparatus
- Flattened membranous sacs (cisternae).
- Modifies ER products, synthesizes macromolecules, and sorts/packages proteins.
Chloroplasts
- Found in plant cells, conduct photosynthesis.
Peroxisomes
- Involved in lipid metabolism and detoxification.
Extracellular Structures
Plant Cell Walls
- Provide structural support.
Animal Extracellular Matrix (ECM)
- Provides structural and biochemical support to surrounding cells.
Intercellular Junctions
- Facilitate cell communication and physical contact.
Types (animal/plant)
- Tight junctions, gap junctions, desmosomes in animal cells; plasmodesmata in plant cells.
Plant Plasmodesmata
- Membrane-lined pores that allow communication and transport of small molecules. (ions, hormones, proteins)
Animal Cell Junctions
- Tight junctions, desmosomes, and gap junctions.
Plasma Membrane (Details)
- Separates cells from surroundings; selectively permeable.
- Composed of a phospholipid bilayer with embedded proteins.
Membrane Proteins
- Integral (spanning membrane) and peripheral (attached to surface) proteins.
Membrane Fluidity
- Cholesterol maintains membrane stability; varies with temperature.
Passive Transport
- Movement of substances down a concentration gradient without energy input.
- Types: simple diffusion, facilitated diffusion, osmosis
Active Transport
- Movement of substances against concentration gradients, requiring energy.
- Types: primary (direct) and secondary (indirect) active transport
- Example: Sodium-potassium pump.
Bulk Transport
- Movement of large materials.
- Exocytosis (materials out) and endocytosis (materials in). (types: phagocytosis, pinocytosis, receptor-mediated endocytosis)
Cell: The Smallest Functional Unit of Life
Nucleic Acids
- Carry genetic information (DNA/RNA).
Cell Membrane
- Regulates the internal environment of the cell.
Cell Wall (Plants)
- Provides protection and support.
Gene
- A segment of DNA that serves as a blueprint for biological traits.
Organelles
- Carry out ATP production, protein synthesis, and cellular structure maintenance. (cytosol, cytoplasm, cytoskeleton, ATP-producing structures)
Cell Cycle
- A series of events leading to cell division: Growth, DNA replication, chromosome distribution.
G1, S, G2, M Phases
- Key phases of the eukaryotic cell cycle.
Prokaryotic Cells
- Use binary fission.
Eukaryotic Cells
- Have complex cycles with distinct phases.
Spindle Apparatus
- Made of microtubules; separates chromatids during cell division.
Centrosomes and Centrioles
- Organize microtubules.
Kinetochore
- Protein complexes that attach chromosomes to spindle fibers.
Cleavage Furrow/ Cell Plate
- Structures formed during cytokinesis to divide cytoplasm (animal/plant).
Stages of Mitosis
- Prophase, Prometaphase, Metaphase, Anaphase, Telophase
Organisms
- Organisms have complex organization.
- Respond to environmental stimuli.
- Grow and develop.
- Maintain internal balance (homeostasis).
Bioenergetics
- Study of energy transformations.
Laws of Thermodynamics
- First Law: Energy conservation.
- Second Law: Entropy increases.
Role of Enzymes
- Lower activation energy; bind to substrates.
ATP (Adenosine Triphosphate)
- Primary energy carrier in cells.
- Generated through substrate-level phosphorylation and chemiosmosis.
- Releases energy via hydrolysis.
Chemiosmosis
- ATP synthesis through electron transport.
Redox Reactions
- Key to ATP production in metabolic pathways.
Catalysts
- Enzymes speed up reactions.
DNA Replication
- DNA molecule is copied to produce two identical molecules.
- Semiconservative Replication.
DNA Replication (details)
- Enzymes involved: DNA helicase, DNA polymerase, DNA ligase.
- Replication fork.
Transcription
- DNA is used as a template to produce mRNA.
Translation
- mRNA is translated into protein at ribosomes.
Central Dogma
- Information flows from genotype (DNA) to phenotype (protein) through RNA.
Translation (details)
- Ribosomes, tRNA, mRNA, codons, anticodons.
Elongation (Translation)
- Ribosome reads mRNA (codon-by-codon), forms peptide bonds.
Termination (Translation)
- Stop codon signals; polypeptide released; ribosome dissociates.
Cellular Respiration
- Breakdown of glucose to generate ATP in mitochondria.
- Aerobic and anaerobic respiration. (glycolysis, citric acid cycle, electron transport chain).
Photosynthesis
- Light-dependent and light-independent reactions. (light reactions, Calvin cycle).
Chloroplast Structure
- Thylakoids, grana, stroma.
Carbon Fixation (Calvin Cycle)
- Location, process of fixing carbon dioxide.
Products of Photosynthesis
- ATP and NADPH (light reactions), and oxygen (released as a byproduct)
- Sugars, starch, or other organic compounds (formed in the Calvin cycle)
Meiosis
- Cell division reducing chromosome number; occurs in sexually reproducing organisms.
- Resulting in genetically distinct haploid cells (gametes).
- Stages: Meiosis I and Meiosis II.
- Events like crossing over and independent assortment.
Meiosis I (details)
- Prophase I, Metaphase I, Anaphase I, Telophase I
Meiosis II (details)
- Prophase II, Metaphase II, Anaphase II, Telophase II
Gametes
- Reproductive cells (sperm and egg) with half the genetic material (haploid).
Zygote
- Fertilized egg; restores diploid number.
Bivalent/Tetrad
- Definition and significance during meiosis.
Crossing Over
Haploid/Diploid
- Definitions and significance.
Genetic Variation
- Sources of variation in meiosis
Independent Assortment
- Basis for genetic diversity.
Chiasmata, Synapsis, Synaptonemal Complex
- Definitions.
Reduction Division (Meiosis)
- Significance.
Genetic Recombination
- Importance in evolution.
Mitosis Outcomes
- Two genetically identical diploid daughter cells.
- Importance in growth and repair.
G1, G2, and Metaphase Checkpoints
- Function & importance.
Growth and Development
- Importance of mitosis.
Prophase, Prometaphase, Metaphase, Anaphase, and Telophase (Mitosis)
- Stages of mitosis
Prokaryotic Chromosomes
- Circular chromosomes
Cytokinesis (Mitosis/Meiosis)
- Cytoplasm division.
Mendelian Genetics
- Laws of Segregation and Independent Assortment.
Dominant/Recessive Alleles
- Definitions of dominance and recessiveness.
Genotype/Phenotype
- Definitions of each.
Chromosomal Genetics
- Study of genes on chromosomes.
Homologous Chromosomes
- Definition of homologous pair.
Sex Chromosomes, Sex-Linked Traits
- Example of sex-linked trait and inheritance patterns.
Incomplete Dominance/Codominance/Polygenic Inheritance
- Definitions of each.
Mitochondrial Inheritance
Chromosomal Disorders
Molecular Biology Techniques
- PCR, Gel Electrophoresis, RNA-seq, CRISPR-Cas9.
DNA Replication (detailed stages)
- Origins of replication.
- Leading and lagging strands.
- Okazaki fragments.
- Proofreading and repair mechanisms.
Transcription
- Initiation, elongation and termination
RNA processing
- Types of modifications. (5' Capping, Polyadenylation, splicing)
Gene Regulation (transcriptional)
- Regulation through transcription factors and epigenetic modifications.
Post-translational Modifications
- Further modification of protein chains.
Epigenetic Modifications
- How gene expression can be altered without changing the DNA sequence.
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