Cell Biology (PB 121) - Gene Expression and Regulation Lecture

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Questions and Answers

What is the main function of DNA ligase in DNA synthesis?

  • Adds nucleotides to the growing DNA strand
  • Separates the double helix for replication
  • Seals the single-strand nick between the nascent chain and Okazaki fragments (correct)
  • Initiates the replication process

What is the function of methylation of histones in gene expression?

  • Activates gene expression
  • Initiates transcription
  • Mutes gene expression (correct)
  • Promotes translation

Which type of RNA is involved in linking mRNA codons to corresponding amino acids?

  • Messenger RNA (mRNA)
  • Ribosomal RNA (rRNA)
  • Micro RNA (miRNA)
  • Transfer RNA (tRNA) (correct)

In which phase of the cell cycle does DNA synthesis occur?

<p>S phase (B)</p> Signup and view all the answers

What is the main function of micro RNA (miRNA) in gene regulation?

<p>Regulates protein expression (D)</p> Signup and view all the answers

Which type of cells contain 40S and 60S ribosomal subunits?

<p>Eukaryotic cells (B)</p> Signup and view all the answers

Which enzyme is responsible for initiating the synthesis of RNA primers during replication?

<p>DNA primase (C)</p> Signup and view all the answers

What is the main function of helicases in the replication process?

<p>Relieve torsional strain in DNA (C)</p> Signup and view all the answers

What role do single-strand binding proteins (SSBs) play in DNA replication?

<p>Prevent reannealing of ssDNA strands (B)</p> Signup and view all the answers

Which class of proteins is involved in deoxynucleotide polymerization during DNA replication?

<p>Helicases (D)</p> Signup and view all the answers

What is the purpose of topoisomerase in DNA replication?

<p>Relieve torsional strain in DNA (A)</p> Signup and view all the answers

Which step involves the identification of the origins of replication in eukaryotic DNA?

<p>Removal of nucleosomes &amp; unwinding of dsDNA (A)</p> Signup and view all the answers

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