Cell Biology (PB 121) - Gene Expression and Regulation Lecture
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Questions and Answers

What is the main function of DNA ligase in DNA synthesis?

  • Adds nucleotides to the growing DNA strand
  • Separates the double helix for replication
  • Seals the single-strand nick between the nascent chain and Okazaki fragments (correct)
  • Initiates the replication process
  • What is the function of methylation of histones in gene expression?

  • Activates gene expression
  • Initiates transcription
  • Mutes gene expression (correct)
  • Promotes translation
  • Which type of RNA is involved in linking mRNA codons to corresponding amino acids?

  • Messenger RNA (mRNA)
  • Ribosomal RNA (rRNA)
  • Micro RNA (miRNA)
  • Transfer RNA (tRNA) (correct)
  • In which phase of the cell cycle does DNA synthesis occur?

    <p>S phase</p> Signup and view all the answers

    What is the main function of micro RNA (miRNA) in gene regulation?

    <p>Regulates protein expression</p> Signup and view all the answers

    Which type of cells contain 40S and 60S ribosomal subunits?

    <p>Eukaryotic cells</p> Signup and view all the answers

    Which enzyme is responsible for initiating the synthesis of RNA primers during replication?

    <p>DNA primase</p> Signup and view all the answers

    What is the main function of helicases in the replication process?

    <p>Relieve torsional strain in DNA</p> Signup and view all the answers

    What role do single-strand binding proteins (SSBs) play in DNA replication?

    <p>Prevent reannealing of ssDNA strands</p> Signup and view all the answers

    Which class of proteins is involved in deoxynucleotide polymerization during DNA replication?

    <p>Helicases</p> Signup and view all the answers

    What is the purpose of topoisomerase in DNA replication?

    <p>Relieve torsional strain in DNA</p> Signup and view all the answers

    Which step involves the identification of the origins of replication in eukaryotic DNA?

    <p>Removal of nucleosomes &amp; unwinding of dsDNA</p> Signup and view all the answers

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