Cell Biology: Nuclear Structure and Function

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Questions and Answers

What structural feature of the nuclear envelope helps regulate the transport of molecules?

  • Chromatin structure
  • Lipid bilayer composition
  • Nuclear pores (correct)
  • Nuclear lamina

Which component is directly responsible for ribosomal RNA synthesis?

  • Nuclear pores
  • Nuclear envelope
  • Nucleolus (correct)
  • Chromatin

What is the primary function of the nuclear lamina?

  • Enhances nuclear membrane permeability
  • Facilitates genetic material replication
  • Provides structural support (correct)
  • Regulates protein synthesis

How many membranes constitute the nuclear envelope?

<p>Double membrane (C)</p> Signup and view all the answers

What is the composition of chromatin?

<p>DNA and proteins (A)</p> Signup and view all the answers

Which structure is directly attached to the inner nuclear membrane?

<p>Nuclear lamina (C)</p> Signup and view all the answers

What role do nuclear pores play in relation to chromatin?

<p>Regulate chromatin accessibility (D)</p> Signup and view all the answers

Where is the nucleolus positioned within the nucleus?

<p>Within the nucleoplasm (D)</p> Signup and view all the answers

What are the small and large structures formed in the nucleolus referred to as?

<p>Ribosomal subunits (D)</p> Signup and view all the answers

In what form is the genetic material organized during interphase?

<p>Chromatin (D)</p> Signup and view all the answers

What is the primary function of chloroplasts in plant cells?

<p>Capture light energy for photosynthesis (C)</p> Signup and view all the answers

Which feature is unique to peroxisomes compared to other organelles?

<p>Involvement in oxidative stress protection (B)</p> Signup and view all the answers

Which type of fiber in the cytoskeleton is the thickest?

<p>Microtubules (D)</p> Signup and view all the answers

What is one of the primary roles of the extracellular matrix (ECM) in animal cells?

<p>Supporting cell movement and signaling (B)</p> Signup and view all the answers

How do tight junctions function in cellular structures?

<p>They prevent fluid movement between adjacent cells (B)</p> Signup and view all the answers

Which structure is primarily responsible for detoxifying chemicals in the cell?

<p>Smooth endoplasmic reticulum (D)</p> Signup and view all the answers

What is the main function of ribosomes during protein synthesis?

<p>Translating mRNA into proteins (D)</p> Signup and view all the answers

Which of the following is NOT a component of the cytoskeleton?

<p>Cellulose fibers (C)</p> Signup and view all the answers

Which cellular structure is primarily responsible for the movement of organelles?

<p>Microtubules (D)</p> Signup and view all the answers

Which component of the endomembrane system is responsible for the modification and sorting of proteins?

<p>Golgi apparatus (D)</p> Signup and view all the answers

What is the internal fluid of mitochondria called?

<p>Matrix (C)</p> Signup and view all the answers

What type of junction allows for the passage of small molecules and ions directly between neighboring cells?

<p>Gap junctions (C)</p> Signup and view all the answers

Which structure contains hydrolytic enzymes and is involved in breaking down cellular waste?

<p>Lysosomes (C)</p> Signup and view all the answers

Which of the following statements about chlorophyll is true?

<p>It gives chloroplasts their green color (A)</p> Signup and view all the answers

What structural characteristic differentiates desmosomes from tight junctions?

<p>Desmosomes anchor adjacent cells together (A)</p> Signup and view all the answers

In which location would you find bound ribosomes?

<p>On the rough endoplasmic reticulum (D)</p> Signup and view all the answers

What process allows lysosomes to recycle the cell's own organelles?

<p>Autophagy (B)</p> Signup and view all the answers

Which of the following statements is true regarding mitochondria and chloroplasts?

<p>Both contain their own DNA (B)</p> Signup and view all the answers

What type of molecules does the Golgi apparatus primarily modify and package?

<p>Macromolecules (D)</p> Signup and view all the answers

What distinguishes rough endoplasmic reticulum from smooth endoplasmic reticulum?

<p>Presence of ribosomes (D)</p> Signup and view all the answers

What is the primary difference in DNA packaging between prokaryotic and eukaryotic cells?

<p>Eukaryotic cells have DNA packaged as multiple, linear chromosomes, while prokaryotic cells have a single, circular chromosome. (B)</p> Signup and view all the answers

Which of the following statements accurately describes the cytoplasm of eukaryotic cells?

<p>Cytoplasm is located between the plasma membrane and the nucleus. (B)</p> Signup and view all the answers

Which of the following is NOT a characteristic of prokaryotic cells?

<p>Have membrane-bound organelles. (B)</p> Signup and view all the answers

In eukaryotic cells, what is the primary function of the ribosomes?

<p>To synthesize proteins. (B)</p> Signup and view all the answers

What type of reproduction do prokaryotic cells primarily use?

<p>Binary fission. (A)</p> Signup and view all the answers

Which of the following organelles is commonly found in eukaryotic cells but not in prokaryotic cells?

<p>Chloroplast. (B)</p> Signup and view all the answers

What structural component is necessary for the cell membrane of all cells, including prokaryotic and eukaryotic cells?

<p>Phospholipid bilayer. (A)</p> Signup and view all the answers

Which of the following statements correctly describes the size difference between eukaryotic and prokaryotic cells?

<p>Eukaryotic cells are generally larger than prokaryotic cells. (D)</p> Signup and view all the answers

What is the role of the nucleus in eukaryotic cells?

<p>To house most of the cell's DNA. (D)</p> Signup and view all the answers

Which of the following accurately describes the plasma membrane's function?

<p>Serves as a selective barrier for regulating entry and exit of substances. (B)</p> Signup and view all the answers

Flashcards

Cell

The basic structural and functional unit of life; the simplest collection of matter that is alive.

Cell Theory

The theory stating that all organisms are made up of cells and all cells come from pre-existing cells.

Prokaryotic Cell

A type of cell that lacks a true nucleus and other membrane-bound organelles.

Eukaryotic Cell

A type of cell that has a true nucleus and other membrane-bound organelles.

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Plasma Membrane

A double layer of phospholipids that forms the outer boundary of a cell, regulating the passage of substances.

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DNA

The genetic material of a cell, usually found in the nucleus of eukaryotic cells.

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Ribosomes

Small complexes within cells where protein synthesis takes place.

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Cytosol

The semifluid substance within the plasma membrane of a cell.

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Nucleoid Region

A non-membrane bound region in prokaryotic cells where DNA is located.

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Binary Fission

A type of asexual reproduction in prokaryotic cells where DNA is copied and the cell divides into two identical cells.

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What is the nuclear envelope?

The nuclear envelope is a double membrane that surrounds the nucleus, separating it from the cytoplasm. It plays a crucial role in controlling the movement of molecules between the nucleus and the cytoplasm.

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What is the structure of the nuclear envelope?

Each membrane of the nuclear envelope is composed of a lipid bilayer, a double layer of lipids. This structure helps to maintain the integrity of the nucleus and regulate the passage of molecules.

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What are nuclear pores?

Nuclear pores are channels or openings in the nuclear envelope that allow the selective passage of molecules into and out of the nucleus. They regulate the transport of essential substances like proteins and RNA.

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What is chromatin?

Chromatin is the complex of DNA and proteins that make up the genetic material within the nucleus. It's organized into long strands, allowing for efficient storage and access to genetic information.

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What is the nucleolus?

The nucleolus is a dense region within the nucleus where ribosomal RNA (rRNA) synthesis occurs. rRNA is essential for the construction of ribosomes, the cellular machinery responsible for protein synthesis.

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What is the nuclear lamina?

The nuclear lamina is a network of proteins that lines the inner surface of the nuclear envelope. It provides structural support for the nucleus, helps maintain its shape, and anchors chromatin.

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How is the nuclear envelope connected to the ER?

The nuclear envelope is attached to the endoplasmic reticulum (ER), a network of membranes involved in protein synthesis and transport. This connection facilitates the movement of molecules between the nucleus and the ER.

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How is the outer membrane of the nuclear envelope connected to the ER?

The outer membrane of the nuclear envelope is continuous with the endoplasmic reticulum (ER), further emphasizing the close relationship between these structures.

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What is the relationship between the nuclear envelope and the ER?

The nuclear envelope and the ER are essential for the synthesis, processing, and transport of proteins within the cell. They work together to ensure the proper functioning of the cell.

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What is the role of nuclear pores in the movement of molecules?

The nuclear pores regulate the entry and exit of molecules from the nucleus, facilitating the flow of genetic information and essential cellular components.

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What is the endoplasmic reticulum (ER)?

The endoplasmic reticulum (ER) is a network of membranes that extends throughout the cytoplasm of eukaryotic cells. It's involved in the synthesis of lipids and proteins, as well as detoxification and calcium storage.

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What does smooth ER synthesize?

Smooth ER lacks ribosomes and is involved in the synthesis of lipids, detoxification, and calcium storage in muscle cells.

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What is the role of rough ER in protein synthesis?

Rough ER has ribosomes attached to its surface and is involved in the production of proteins.

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What is the function of the Golgi apparatus?

The Golgi apparatus is a stack of flattened membranous sacs that modifies, sorts, and packages proteins and lipids for secretion or use within the cell.

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What are lysosomes and what do they do?

Lysosomes are membrane-bound organelles containing hydrolytic enzymes that break down cellular waste, worn-out organelles, and ingested substances.

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What is the role of mitochondria in the cell?

Mitochondria are double-membraned organelles responsible for cellular respiration, which produces ATP, the cell's energy currency.

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What are chloroplasts and what do they do?

Chloroplasts are double-membraned organelles found in plants and algae that carry out photosynthesis, the process of converting light energy into chemical energy.

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What are the components of the endomembrane system?

The nuclear envelope, ER, Golgi apparatus, lysosomes, and plasma membrane are all part of the endomembrane system, which works together to modify, package, and transport proteins and lipids within the cell.

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Why are mitochondria and chloroplasts not considered part of the endomembrane system?

Mitochondria and chloroplasts are not part of the endomembrane system, but they are still important organelles with double membranes. They also have their own DNA and produce proteins via ribosomes.

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What is the mechanism behind the acidic internal pH of lysosomes?

A proton pump in the lysosomal membrane imports protons from the cytosol, using ATP hydrolysis, thereby maintaining the acidic internal pH of the lysosome.

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Thylakoid

A membranous sac inside a chloroplast where light energy is captured during photosynthesis.

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Granum

A stack of thylakoids within a chloroplast.

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Stroma

The internal fluid of a chloroplast, surrounding the thylakoids.

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Peroxisomes

Organelles responsible for breaking down fatty acids, alcohol, and protecting cells from reactive oxygen species.

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Reactive Oxygen Species (ROS)

Harmful molecules produced as a byproduct of normal cellular metabolism, radiation, or environmental factors.

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Cytoskeleton

A network of protein fibers extending throughout the cytoplasm of a cell, providing structural support, aiding movement, and organizing cellular activities.

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Microtubules

The thickest components of the cytoskeleton, involved in cell shape, organelle movement, and cell division.

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Microfilaments

The thinnest components of the cytoskeleton, involved in cell shape, muscle contraction, and cytoplasmic streaming.

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Intermediate Filaments

Fibers with diameters between microtubules and microfilaments, involved in providing structural support and maintaining cell shape.

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Extracellular Matrix (ECM)

A complex network of molecules outside the plasma membrane of animal cells, providing structural support, cell movement, and cell-to-cell signaling.

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Study Notes

Cell Overview

  • Cells are the fundamental units of life, forming the simplest collection of matter that is alive
  • The cell theory states that all organisms consist of cells and all cells come from pre-existing cells

Cell Types

  • Cells are categorized as prokaryotic or eukaryotic
  • Prokaryotic cells are found in bacteria and archaea
  • Eukaryotic cells are found in protists, fungi, animals, and plants

Cell Features

  • Plasma (cell) membrane: Separates the cell's interior from its outside environment, acts as a selective barrier.
  • Biological Membranes: Generally structured as a double layer (bilayer) of phospholipids
  • DNA: The genetic material, contained within one or more chromosomes.
  • Ribosomes: Small complexes where protein synthesis takes place.
  • Cytosol (cytoplasm): A semifluid substance within the plasma membrane.

Prokaryotic Cells

  • Prokaryotic cells lack membrane-bound organelles
  • DNA is found in a non-membrane-bound nucleoid region
  • Most prokaryotes have one circular chromosome
  • Cell division occurs via binary fission

Eukaryotic Cells

  • Eukaryotic cells contain membrane-bound organelles
  • DNA is enclosed within a membrane-bound nucleus
  • DNA is packaged into multiple linear chromosomes
  • Cell division typically occurs via mitosis

Nucleus

  • The nucleus houses most of the cell's DNA, acting as the "information central"
  • Encloses the nucleus and separates it from the cytoplasm
  • The nuclear envelope is a double membrane with lipid bilayers that regulate entry/exit of molecules.
  • Chromatin is formed by DNA and proteins
  • The nucleolus is inside the nucleus and responsible for ribosome subunit production.

Ribosomes

  • Ribosomes are particles made of ribosomal RNA and protein
  • Carry out protein synthesis in two locations: free ribosomes in cytoplasm and bound ribosomes on endoplasmic reticulum or nuclear envelope.

Endomembrane System

  • The endomembrane system is a network of membranes within eukaryotic cells:
    • Includes nuclear envelope, endoplasmic reticulum (smooth and rough), Golgi apparatus, lysosomes, and plasma membrane.
  • Its role is in modifying, packaging, and transporting lipids and proteins.

Endoplasmic Reticulum

  • accounts for about 50% of the total membrane in many eukaryotic cells
  • The ER membrane continuous with (or connected to) the nuclear envelope
  • There are two distinctive regions: smooth ER and rough ER:
    • Smooth ER lacks ribosomes, involved in lipid synthesis (and steroid hormones) and detoxification.
    • Rough ER has ribosomes, involved in protein synthesis.

Golgi Apparatus

  • Consists of flattened membranous sacs (cisternae)
  • Modifies, sorts, and packages macromolecules (for secretion or use within the cell)

Lysosomes

  • Membrane-bound organelles containing hydrolytic enzymes
  • Digest macromolecules like proteins, fats, nucleic acids, and carbohydrates.
  • Involved in autophagy (recycling old organelles).

Mitochondria and Chloroplasts

  • Mitochondria: Sites of cellular respiration (generating ATP via energy conversion). Have a smooth outer membrane and folded inner membrane called cristae. Internal fluid is called matrix. Have their own DNA, proteins made by free ribosomes, and are not part of endomembrane system
  • Chloroplasts (plant and algae): Sites of photosynthesis. Have double membrane, internal thylakoids, and stroma. Contain their own DNA, proteins made by free ribosomes, and are not part of endomembrane system
  • Number of mitochondria and chloroplasts vary per cell type

Peroxisomes

  • Not part of endomembrane system; have plasma membrane and crystalline core, which contains enzymes
  • Important in the breakdown of fatty acid molecules, alcohols, and protection against reactive oxygen species (ROS).

Cytoskeleton

  • A network of protein fibers extending throughout the cytoplasm.
  • Maintains cell shape and structure.
  • Made up of three types of molecular structures: microtubules, microfilaments (actin filaments), and intermediate filaments.

Cell Motility

  • Includes: movement of cilia or flagella (microtubules) and pseudopodia (microfilaments) for cell extensions to move.

Cell Division

  • Involves microtubules (for mitosis and meiosis) and microfilaments (especially in cytokinesis for animal cells) for distributing cytoplasm into two new daughter cells.

Extracellular Components

  • Most cells secrete materials external to their plasma membrane, including:
    • Cell walls (plants, prokaryotes, fungi, some protists)
      • Protect cell, maintain shape, and prevent excessive water uptake.
      • Made of cellulose in plants, other polysaccharides and proteins.
    • Extracellular matrix (ECM) (animal cells):
      • Glycoproteins like collagen, proteoglycans, and fibronectin
      • Support structure and cell-to-cell signaling.
      • Integrins connect ECM to cell membrane.
    • Intercellular junctions (between cells):
      • Plasmodesmata: (plant cells) Perforate cell walls, allow passage of water, small solutes, and some RNA and proteins.
      • Tight junctions: (animal cells) Prevent leakage between cells
      • Desmosomes: (animal cells) Fasten cells together
      • Gap junctions: (animal cells) Provide cytoplasmic channels between adjacent cells for communication.

Plant vs. Animal Cells

  • Summary of key differences: plant cells have cell walls, chloroplasts, a large central vacuole, use starch for energy (not glycogen), and mostly lack lysosomes. Animal cells have no cell wall, lack chloroplasts, but the extracellular matrix is their structural complement, have small vacuoles, and use glycogen as primary energy source. Plant and animal cells both have lysosomes generally.

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