Compendium 5
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Questions and Answers

What is the primary process that allows neurotransmitter release at the synapse?

Exocytosis allows neurotransmitter release by secretory vesicles fusing with the plasma membrane.

Explain the difference between phagocytosis and pinocytosis.

Phagocytosis involves engulfing large particles, while pinocytosis is the engulfing of small particles and fluids.

What drives the movement of water in osmosis?

Osmosis is driven by the concentration of solutes, as water moves from low solute concentration to high solute concentration.

Describe what happens to red blood cells in a hypertonic solution.

<p>In a hypertonic solution, red blood cells lose water, leading to crenation and deformation.</p> Signup and view all the answers

What is facilitated diffusion and how does it differ from active transport?

<p>Facilitated diffusion is the process where solutes bind to specific transporters and move down a concentration gradient without energy, while active transport moves solutes against a gradient and requires energy, usually from ATP.</p> Signup and view all the answers

Describe the role of ion channels in facilitated diffusion.

<p>Ion channels are specific proteins that allow selective ions to pass through the membrane, facilitating their movement in accordance with electrochemical gradients.</p> Signup and view all the answers

How do aquaporins facilitate the movement of water across the plasma membrane?

<p>Aquaporins are transmembrane proteins that function as channels, allowing water to pass through the lipid bilayer efficiently.</p> Signup and view all the answers

Explain how gated protein channels function in the cell membrane.

<p>Gated protein channels only open in response to specific signals, allowing ions to pass when active, thus regulating ion flow based on cellular needs.</p> Signup and view all the answers

What is the relationship between osmotic pressure and solute concentration?

<p>Osmotic pressure is proportional to the concentration of solute particles that cannot cross the membrane.</p> Signup and view all the answers

What is exocytosis, and in which types of cells is it primarily observed?

<p>Exocytosis is the process by which large molecules are expelled from the cell, primarily observed in secretory cells such as those in glands.</p> Signup and view all the answers

What occurs during receptor-mediated endocytosis?

<p>Receptor-mediated endocytosis involves receptor molecules binding to specific substances, leading to vesicle formation and transport into the cell.</p> Signup and view all the answers

Define isosmotic and its effect on cell volume.

<p>Isosmotic solutions do not change cell volume as there is no net movement of water across the membrane.</p> Signup and view all the answers

How does the sodium-potassium pump operate, and why is it important?

<p>The sodium-potassium pump transports Na+ out and K+ into the cell against their concentration gradients, using ATP, and is crucial for maintaining cellular ion balance.</p> Signup and view all the answers

What is the primary energy source for primary active transport?

<p>The primary energy source for primary active transport is ATP, which is directly utilized during the process of ATP hydrolysis.</p> Signup and view all the answers

What is facilitated diffusion and how does it differ from active transport?

<p>Facilitated diffusion is the passive transport of molecules across the membrane via carrier proteins, while active transport requires energy to move substances against their concentration gradient.</p> Signup and view all the answers

What happens to cells in a hypotonic solution?

<p>Cells placed in a hypotonic solution will gain water, risking haemolysis or bursting.</p> Signup and view all the answers

Describe secondary active transport and its reliance on primary active transport.

<p>Secondary active transport uses the energy stored in the concentration gradients of ions, established by primary active transport (like the sodium-potassium pump), to move other substances against their gradients.</p> Signup and view all the answers

Identify the factors that influence the rate of movement during facilitated diffusion.

<p>The rate of movement during facilitated diffusion depends on the steepness of the concentration gradient and the number of available transporter proteins in the membrane.</p> Signup and view all the answers

How do antiporters and symporters operate in secondary active transport?

<p>Antiporters move one ion in one direction while transporting another ion in the opposite direction, whereas symporters move two ions in the same direction, both using gradients created by primary active transport.</p> Signup and view all the answers

What is the significance of having transport proteins that are selective for specific ions?

<p>Selective transport proteins ensure that only specific ions can pass through the membrane, thereby maintaining the essential electrochemical gradients vital for cellular functions.</p> Signup and view all the answers

What role do ion channels play in the plasma membrane?

<p>Ion channels allow specific ions to pass through the plasma membrane, facilitating the transport of charged particles across the lipid bilayer.</p> Signup and view all the answers

How does exocytosis differ from endocytosis?

<p>Exocytosis involves the expulsion of substances from the cell, while endocytosis is the process of engulfing materials into the cell.</p> Signup and view all the answers

What is the primary energy source for active transport mechanisms?

<p>Active transport mechanisms primarily use ATP as their energy source to move substances against their concentration gradient.</p> Signup and view all the answers

Describe facilitated diffusion and its requirements.

<p>Facilitated diffusion is a passive transport process that requires integral membrane proteins to help move water-soluble substances across the membrane.</p> Signup and view all the answers

What is osmosis and how does it differ from diffusion?

<p>Osmosis is the movement of water from an area of low solute concentration to an area of high solute concentration, while diffusion pertains to the movement of any substance from high to low concentration.</p> Signup and view all the answers

What substances typically pass through the lipid bilayer via simple diffusion?

<p>Lipid-soluble substances, such as respiratory gases (O2, CO2), small alcohols, and urea, can pass through the lipid bilayer via simple diffusion.</p> Signup and view all the answers

Explain the differences between the three types of passive transport.

<p>The three types of passive transport include simple diffusion (lipid soluble), channel-mediated diffusion (water soluble), and facilitated diffusion (water soluble with carriers).</p> Signup and view all the answers

What function do aquaporins serve in the plasma membrane?

<p>Aquaporins are transmembrane proteins that function as channels specifically for the rapid transport of water molecules across the plasma membrane.</p> Signup and view all the answers

How do integral proteins differ from peripheral proteins in the plasma membrane?

<p>Integral proteins span the entire width of the membrane and are involved in transport, whereas peripheral proteins are located on the inside or outside surface and provide structural support.</p> Signup and view all the answers

What factors influence the permeability of the plasma membrane?

<p>Permeability of the plasma membrane is influenced by factors such as lipid solubility, molecular size, and the presence of driving forces like concentration gradients.</p> Signup and view all the answers

What is the primary role of ion channels in cellular function?

<p>Ion channels facilitate the passive movement of ions across the cell membrane, maintaining electrochemical gradients essential for processes like action potentials.</p> Signup and view all the answers

Describe how exocytosis differs from endocytosis in terms of cellular transport.

<p>Exocytosis is the process by which cells expel materials, while endocytosis involves the uptake of substances into the cell.</p> Signup and view all the answers

What is active transport, and why is it essential for cellular homeostasis?

<p>Active transport is the process of moving substances against their concentration gradient using energy, typically in the form of ATP.</p> Signup and view all the answers

In what way does facilitated diffusion differ from simple diffusion?

<p>Facilitated diffusion requires specific transport proteins to help move molecules across the membrane, while simple diffusion directly relies on the concentration gradient.</p> Signup and view all the answers

Explain how osmosis is crucial for maintaining cell integrity.

<p>Osmosis is the movement of water across a semipermeable membrane from an area of lower solute concentration to higher, helping maintain cellular turgor pressure.</p> Signup and view all the answers

What role do ATP and ion pumps play in active transport mechanisms?

<p>ATP is used as an energy source to power ion pumps that move ions against their concentration gradients.</p> Signup and view all the answers

How does the concentration gradient influence facilitated diffusion?

<p>Facilitated diffusion occurs along the concentration gradient, meaning substances move from areas of high concentration to low without energy input.</p> Signup and view all the answers

Outline the key difference between isotonic, hypertonic, and hypotonic solutions.

<p>Isotonic solutions have equal solute concentrations on both sides of the membrane, hypertonic solutions have higher solute concentrations outside the cell, and hypotonic solutions have lower concentrations outside.</p> Signup and view all the answers

What is the significance of the sodium-potassium pump in cellular function?

<p>The sodium-potassium pump actively transports sodium out and potassium into the cell, maintaining the electrochemical gradient necessary for nerve impulse transmission.</p> Signup and view all the answers

How do cells utilize endocytosis for nutrient uptake?

<p>Cells use endocytosis to engulf extracellular substances, forming vesicles that bring nutrients into the cell for energy production.</p> Signup and view all the answers

What are the main components of the plasma membrane?

<p>The plasma membrane is primarily composed of phospholipids, proteins, and cholesterol.</p> Signup and view all the answers

How does cholesterol affect the structure of the plasma membrane?

<p>Cholesterol molecules provide rigidity to the membrane by inserting themselves between phospholipid molecules.</p> Signup and view all the answers

What is the role of integral proteins in the plasma membrane?

<p>Integral proteins span the entire width of the membrane and facilitate the transport of substances across it.</p> Signup and view all the answers

Define osmosis in the context of the plasma membrane.

<p>Osmosis is the movement of water from a low solute concentration to a high solute concentration across a selectively permeable membrane.</p> Signup and view all the answers

What type of transport does water undergo when passing through the plasma membrane?

<p>Water can pass through the plasma membrane by diffusion through the lipid bilayer or through aquaporins.</p> Signup and view all the answers

Explain the difference between passive and active transport.

<p>Passive transport does not require energy and occurs along a concentration gradient, while active transport requires ATP and moves substances against a gradient.</p> Signup and view all the answers

What are the three types of passive transport mentioned?

<p>The three types of passive transport are simple diffusion, channel-mediated diffusion, and facilitated diffusion.</p> Signup and view all the answers

How does molecular size influence permeability in the plasma membrane?

<p>Molecular size affects permeability as smaller molecules can more easily diffuse through the lipid bilayer or protein channels.</p> Signup and view all the answers

What driving force is commonly responsible for diffusion across the lipid bilayer?

<p>A concentration gradient is typically the driving force for diffusion across the lipid bilayer.</p> Signup and view all the answers

What role do peripheral proteins play in relation to the plasma membrane?

<p>Peripheral proteins are attached to the inner or outer surface of the membrane and assist in signaling and structural support.</p> Signup and view all the answers

What is a key characteristic of transporter proteins involved in facilitated diffusion?

<p>Transporter proteins bind specifically to a solute on one side of the membrane and release it on the other side.</p> Signup and view all the answers

How does the steepness of the concentration gradient affect the rate of facilitated diffusion?

<p>A steeper concentration gradient increases the rate of facilitated diffusion, allowing more solutes to be transported quickly.</p> Signup and view all the answers

What distinguishes gated protein channels from continuously open ion channels?

<p>Gated protein channels open and close in response to specific signals, whereas continuously open ion channels remain available for ion passage.</p> Signup and view all the answers

Explain the role of ATP in primary active transport mechanisms.

<p>ATP provides the energy required for primary active transport by undergoing hydrolysis to propel solutes against their concentration gradient.</p> Signup and view all the answers

What occurs during secondary active transport, and how does it relate to primary active transport?

<p>Secondary active transport utilizes the concentration gradients established by primary active transport to move other substances against their gradients.</p> Signup and view all the answers

Describe the process of exocytosis and its significance in cellular function.

<p>Exocytosis involves the movement of large molecules out of the cell via vesicles that fuse with the cell membrane.</p> Signup and view all the answers

In what ways do antiporters and symporters function during secondary active transport?

<p>Antiporters transport two different ions or molecules in opposite directions, while symporters move them in the same direction across the membrane.</p> Signup and view all the answers

What is the primary function of the sodium-potassium pump in cellular transport?

<p>The sodium-potassium pump maintains low sodium and high potassium concentrations inside cells, crucial for cell function.</p> Signup and view all the answers

What types of solutes are typically transported using facilitated diffusion?

<p>Solutes like glucose and fructose are commonly transported into and out of cells via facilitated diffusion.</p> Signup and view all the answers

How do concentration gradients influence the direction and rate of solute movement in facilitated diffusion?

<p>Solutes move from areas of higher concentration to lower concentration, with the rate increasing along a steeper gradient.</p> Signup and view all the answers

What initiates the process of neurotransmitter release at the synapse?

<p>The movement of a secretory vesicle towards the plasma membrane initiates neurotransmitter release.</p> Signup and view all the answers

What is the primary function of endocytosis?

<p>Endocytosis primarily facilitates the movement of large molecules and particles into the cell.</p> Signup and view all the answers

What is osmosis and how does it differ from simple diffusion?

<p>Osmosis is the movement of water from low to high solute concentration, while simple diffusion involves the movement of solutes across a membrane.</p> Signup and view all the answers

Explain what happens to cells in a hypotonic solution.

<p>In a hypotonic solution, cells gain water and may swell or burst due to excess water intake.</p> Signup and view all the answers

How do receptors function in receptor-mediated endocytosis?

<p>Receptor molecules on the cell's surface bind to specific substances, forming a vesicle that is taken into the cell.</p> Signup and view all the answers

What is the role of osmotic pressure in the movement of water?

<p>Osmotic pressure encourages water to move towards the solution with the highest concentration of solutes.</p> Signup and view all the answers

What characterizes an isotonic solution in relation to cell volume?

<p>In an isotonic solution, there is no net movement of water, so cells maintain their normal shape.</p> Signup and view all the answers

Describe the consequence of placing red blood cells in a hypertonic solution.

<p>Red blood cells in a hypertonic solution lose water, leading to crenation or cell dehydration.</p> Signup and view all the answers

What happens during the fusion of a secretory vesicle with the plasma membrane?

<p>The fusion results in the release of the vesicle's contents into the extracellular fluid.</p> Signup and view all the answers

How does the concentration of solutes affect the direction of water movement in osmosis?

<p>Water moves from areas of lower solute concentration to areas of higher solute concentration.</p> Signup and view all the answers

What process involves the breaking down of glucose to release energy in the absence of oxygen?

<p>Glycolysis is the process that breaks down glucose anaerobically.</p> Signup and view all the answers

Why is the breakdown of ATP considered crucial for cellular functions?

<p>ATP provides the energy necessary for muscle contraction, active transport, and cellular movement.</p> Signup and view all the answers

What major products result from one cycle of the Krebs cycle using acetyl CoA?

<p>Each cycle produces 2 CO2, 3 NADH, 1 ATP, and 1 FADH2.</p> Signup and view all the answers

What is the primary difference between aerobic and anaerobic cellular respiration?

<p>Aerobic respiration requires oxygen and produces more ATP, while anaerobic respiration occurs without oxygen and yields less ATP.</p> Signup and view all the answers

What role do NADH and FADH2 play in cellular respiration?

<p>NADH and FADH2 act as electron carriers, transporting electrons to the electron transport chain.</p> Signup and view all the answers

What molecule is formed when pyruvic acid undergoes decarboxylation in the mitochondria?

<p>Acetyl coenzyme A (acetyl CoA) is formed after pyruvic acid is decarboxylated.</p> Signup and view all the answers

How is energy produced during the electron transport chain?

<p>Energy is produced as electrons move through cytochromes, pumping H+ ions to create a proton gradient.</p> Signup and view all the answers

What byproducts are generated during the complete oxidation of glucose?

<p>The byproducts of glucose oxidation are CO2 and H2O.</p> Signup and view all the answers

What is the net energy gain from glycolysis, including produced and used ATP?

<p>The net energy gain from glycolysis is 2 ATP and 2 NADH.</p> Signup and view all the answers

What determines whether pyruvic acid is converted to lactic acid or enters the Krebs cycle?

<p>The availability of oxygen determines its fate; if O2 is present, it enters the Krebs cycle; if not, it is converted to lactic acid.</p> Signup and view all the answers

Study Notes

Membrane Transport

  • Transport across the membrane:
    • Passive transport: movement of substances across a membrane without using cellular energy
      • Simple diffusion: movement of substances across a membrane down a concentration gradient (e.g. lipids, respiratory gases)
      • Facilitated diffusion: movement of substances across a membrane down a concentration gradient with the help of carrier or channel proteins (e.g. glucose, fructose, ions, water)
    • Active transport: movement of substances across a membrane against a concentration gradient, requiring cellular energy (ATP)
  • Facilitated diffusion:
    • A solute binds to a specific transporter on one side of the membrane and is released on the other side.
    • Rate of movement depends on steepness of the concentration gradient and the number of transporter proteins in the membrane (transport maximum).
    • Examples: glucose (out of the cell) and fructose (into the cell).
  • Gated protein channels:
    • Some membrane proteins are ion channels that allow the passage of specific ions.
    • Electrochemical gradient is often the driving force.
    • Some channels are continuously open, while others are gated and open transiently.
    • Transport occurs at a faster rate than facilitated diffusion.
  • Active transport:
    • Requires energy to move solutes against their concentration gradient.
      • Primary active transport: Energy is derived directly from ATP (e.g. sodium-potassium pump).
        • The sodium-potassium pump maintains a low concentration of Na+ and a high concentration of K+ in the cytosol.
        • Accounts for 40% of cellular ATP usage.
      • Secondary active transport: Energy is derived indirectly from ATP, using the concentration gradient of another molecule (e.g. cotransport of Na+ or H+ ions).
        • The concentration gradient of Na+ or H+ is used to drive other substances against their own concentration gradient.

Osmosis and Tonicity

  • Osmosis: Movement of water across a selectively permeable membrane from an area of low solute concentration to an area of high solute concentration.
    • Driving force is the concentration of solutes, not water concentration.
    • Osmotic pressure: The drawing power of a solution to encourage water to move towards it.
    • Water moves to the solution with the highest osmotic pressure (highest solute concentration).
  • Tonicity: A measure of a solution's ability to change the volume of cells by altering their water content.
    • Isotonic solution: No net movement of water; cells maintain their normal shape.
    • Hypertonic solution: Cells lose water and shrink or become dehydrated.
    • Hypotonic solution: Cells gain water and swell, potentially bursting.

Cellular Respiration

  • Metabolism: All chemical reactions in the body.
    • Catabolism: Breakdown of complex molecules into simpler ones.
    • Anabolism: Building up of simple molecules into complex ones.
  • ATP: Adenosine triphosphate, a molecule for temporary energy storage.
    • Used for muscle contraction, active transport, and movement of structures within a cell.
    • Energy is released when the terminal phosphate bond is hydrolyzed.
  • Stages in energy generation:
    • Stage 1: Large molecules are broken down into smaller units (e.g. proteins to amino acids, fats to glycerol and fatty acids, polysaccharides to simple sugars)
    • Stage 2: Smaller units are degraded into key simple compounds important for metabolism.
    • Stage 3: Citric acid cycle (Krebs cycle) and oxidative phosphorylation.
  • Carbohydrate metabolism:
    • Glucose is the primary fuel for energy production.
    • Glucose catabolism occurs in three stages: glycolysis, Krebs cycle, and electron transport chain.
    • Glycolysis: Glucose is broken down into pyruvic acid, producing 2 net ATP and 2 NADH molecules.
    • Krebs cycle: Acetyl CoA (from pyruvic acid) enters the cycle, yielding 2 CO2, 3 NADH, 1 FADH2, and 1 ATP per acetyl CoA molecule.
    • Electron transport chain: NADH and FADH2 deliver electrons, which are passed through a series of carriers, generating ATP via oxidative phosphorylation.

Summary of Cellular Respiration

  • Aerobic respiration produces ATP (energy) from glucose using oxygen.
    • Net ATP production: 36-38 ATP per glucose molecule.
  • Anaerobic respiration occurs in the absence of oxygen and produces much less ATP than aerobic respiration.
    • Glycolysis is the main anaerobic pathway.

Glycolysis

  • Occurs in the cytoplasm.
  • Glucose is broken down into two pyruvate molecules.
  • Net gain of 2 ATP and 2 NADH molecules.
  • Requires 2 ATP molecules to initiate the process.
  • Phases of glycolysis:
    • Phase 1: Sugar activation. Glucose is phosphorylated (adding phosphate groups) using ATP.
    • Phase 2: Sugar cleavage. The 6-carbon sugar is split into two 3-carbon molecules.
    • Phase 3: Oxidation and ATP formation. Phosphate groups are removed from the 3-carbon molecules, forming 4 ATP molecules. The remaining 3-carbon molecules are pyruvate.
  • Fate of pyruvate:
    • In the presence of oxygen: Pyruvate enters the mitochondria for further oxidation in the Krebs cycle.
    • In the absence of oxygen: Pyruvate is converted to lactate (lactic acid fermentation).

Acetyl CoA Formation

  • Pyruvate is converted to acetyl CoA before entering the Krebs cycle.
  • Occurs in the mitochondria.
  • Pyruvate is decarboxylated (loses a carbon atom as CO2).
  • The remaining 2-carbon fragment is attached to Coenzyme A, forming acetyl CoA.

Krebs Cycle (Citric Acid Cycle)

  • Occurs in the matrix of the mitochondria.
  • Acetyl CoA enters the cycle and combines with oxaloacetate to form citric acid (a 6-carbon molecule).
  • Citric acid is broken down through a series of reactions, releasing CO2, NADH, FADH2, and ATP.
  • Oxaloacetate is regenerated at the end of the cycle.

Electron Transport Chain and Oxidative Phosphorylation

  • Occurs on the inner membrane of the mitochondria.
  • Electrons are passed from NADH and FADH2 to a series of electron carriers.
  • Energy is released as electrons move through the chain, creating a proton gradient.
  • Protons (H+) flow back across the membrane through ATP synthase, generating ATP.
  • Oxygen is the final electron acceptor, forming water (H2O).

Lecture 2: Composition of the Plasma Membrane

  • Lipid bilayer: Forms the basic structure of the plasma membrane.
    • Composed of phospholipids, with hydrophilic heads facing the aqueous environment and hydrophobic tails facing each other.
    • Acts as a barrier to most "charged (polar)" and "non lipid soluble" substances.
  • Cholesterol: Inserted between phospholipid molecules to provide rigidity and stability.
  • Proteins:
    • Integral proteins: Span the entire width of the membrane and can function as channels, carriers, or receptors.
    • Peripheral proteins: Located on the inner or outer surface of the membrane and can function as enzymes or anchors.
  • Selective permeability: The membrane allows some substances to pass through while blocking others.
    • Factors affecting permeability: solubility in lipids, driving forces (gradients), and molecular size.

Water Transport

  • Aquaporins: Transmembrane proteins that function as water channels, facilitating water movement across the membrane.
  • Osmosis: The movement of water from an area of low solute concentration to an area of high solute concentration across a selectively permeable membrane.
    • Osmotic pressure: The force exerted by a solution to draw water across the membrane.
    • Tonicity: A measure of a solution's ability to change cell volume by altering water content.

Lecture 3: Introduction to Glycolysis

  • Glycolysis is the first stage of cellular respiration.
  • Occurs in the cytoplasm.
  • Glucose is broken down into two pyruvate molecules.
  • Net gain of 2 ATP molecules and 2 NADH molecules.
  • Key steps in Glycolysis:
    • Glucose activation: Glucose is phosphorylated, using ATP.
    • Sugar cleavage: The 6-carbon sugar is split into two 3-carbon molecules.
    • Oxidation and ATP formation: The 3-carbon molecules are oxidized, generating 4 ATP molecules.

Lecture 4: Formation of Acetyl CoA

  • Pyruvate (3-carbon molecule) is converted to Acetyl CoA (2-carbon molecule) before entering the Krebs Cycle.
  • Occurs in the mitochondria.
  • Pyruvate is decarboxylated (loses a carbon as CO2).
  • The remaining 2-carbon fragment is attached to Coenzyme A, forming Acetyl CoA.

Krebs Cycle (Citric Acid Cycle)

  • Occurs in the mitochondrial matrix.
  • Acetyl CoA combines with oxaloacetate (a 4-carbon molecule) to form citric acid (a 6-carbon molecule).
  • **Citric acid is broken down step-by-step, releasing CO2, NADH, FADH2, and ATP. **
  • Oxaloacetate is regenerated at the end of the cycle.

Electron Transport Chain and Oxidative Phosphorylation

  • Occurs in the inner mitochondrial membrane.
  • NADH and FADH2 deliver electrons to a series of protein carriers.
  • Electrons are passed from one carrier to another, releasing energy to pump protons (H+) across the inner membrane.
  • A proton gradient is established.
  • Protons flow back across the membrane through ATP synthase, generating ATP.
  • Oxygen acts as the final electron acceptor, forming water.

Summary of Cellular Respiration

  • Aerobic respiration produces ATP (energy) from glucose using oxygen.
    • Produces significantly higher ATP per glucose molecule than anaerobic respiration.
  • Anaerobic respiration occurs in the absence of oxygen and produces much less ATP than aerobic respiration.
    • Glycolysis is the main anaerobic pathway.

Plasma Membrane

  • Forms a complete boundary around the cell.
  • Composed of phospholipids, proteins, and cholesterol.
  • The bi-molecular layer of phospholipids forms the basic structure.
  • Cholesterol molecules are inserted between the phospholipid molecules to provide rigidity.
  • Integrates both integral and peripheral proteins.

Membrane Transport

  • Lipid bilayer serves as a highly impermeable barrier to most charged (polar) and non-lipid soluble substances.
  • Integral proteins act as pores, channels, or carriers allowing substances to cross the membrane.
  • Plasma membranes are selectively permeable; some things can pass, while others can't.
  • Permeability is a function of solubility in lipids, driving forces (up or down gradient), and molecular size.
  • Transport can be active (usually against gradient) or passive (usually moving down a gradient).
  • Water-soluble substances require specialised transmembrane proteins to function as channels or carriers.

Types of Passive Transport

  • Diffusion through the lipid bilayer (lipid-soluble) (simple diffusion).
  • Diffusion through the pores/ion channels (water-soluble) (channel mediated).
  • Facilitated diffusion (water-soluble) (carrier mediated).

Active Transport

  • Requires ATP (cellular energy).
  • Water molecules penetrate the membrane by diffusion through the lipid bilayer or through aquaporins (transmembrane proteins) that function as water channels.
  • The movement of water through the membrane is called osmosis, defined as "the movement of water from a low solute concentration to a high solute concentration."

Diffusion Through The Lipid Bilayer

  • Lipid-soluble substances, such as respiratory gases, lipids, small alcohols, and urea, can diffuse across the lipid bilayer (O2/CO2).
  • A concentration gradient is usually the driving force for this type of transport.

Diffusion Across the Lipid Bilayer

  • Water-soluble substances like ions, small sugars, amino acids, and water need integral membrane proteins to move across the cell membrane.
  • Small ions (channels)
  • Water (channels)
  • Sugars and amino acids (facilitated diffusion)
  • A concentration or electrical gradient is often the driving force of this type of transport.

Facilitated Diffusion

  • In facilitated diffusion, a solute binds to a specific transporter on the side of the membrane and is released on the other side.
  • Solutes that move this way include glucose (out of the cell) and fructose (into the cell).
  • The rate of movement depends on the steepness of the concentration gradient and the number of transporter proteins in the membrane (transport maximum).

Gated Protein Channels

  • Some membrane proteins are ion channels.
  • An electrochemical gradient is often the driving force.
  • Ion channels are selective and specific (usually specific to an ion and will only let that ion pass through).
  • Some channels formed by transport proteins are continuously open, but others only open transiently (gated protein channels).
  • Transport occurs at a faster rate compared to facilitated diffusion.

Active Transport

  • Active transport moves solutes against a concentration gradient, requiring energy.
  • Primary active transport derives energy directly from ATP (metabolic ATP hydrolysis).
  • The sodium-potassium ion pump is the most common primary active transport mechanism:
    • It uses 40% of cellular ATP.
    • All cells have thousands of them.
    • Maintains a low concentration of Na+ and a high concentration of K+ in the cytosol.
    • Operates continually.
  • Secondary active transport derives energy indirectly from ATP through cotransport of Na+ or H+ ions.
    • Energy is stored in Na+ or H+ concentration gradients, used to drive other substances against their own concentration gradients.
    • Plasma membranes contain several antiporters and symporters powered by the sodium ion gradient created by the sodium potassium pump.

Membrane Transport of Complex Molecules

  • Exocytosis: movement of large molecules out of the cell; occurs in secretory cells; secretions in vesicles (membrane packets); vesicles fuse with the cell membrane (e.g., neurotransmitter secretion at the synapse).
  • Endocytosis: movement of large molecules and particles into the cell; pinocytosis - engulfing small particles and fluids; phagocytosis - engulfing large particles; receptor-mediated endocytosis - movement of specific substances into the cell involving the caveolae regions of the cell membrane.

Osmosis

  • Water molecules penetrate the membrane by diffusion through the lipid bilayer or through aquaporins (transmembrane proteins) that function as water channels.
  • The movement of water through the membrane is called osmosis, which is defined as the movement of water from a low solute concentration to a high solute concentration.

Osmosis: Driving Forces

  • The driving force is not the water concentration but the concentration of the solutes dissolved within it.
  • Water is the solvent for all solutes and is present at a very high concentration (56 molar).
  • This means that when solutes are dissolved in water, their concentration changes very little.
  • When a solute dissolves in water, it displays an osmotic pressure or drawing power to encourage water to move towards it.
  • Therefore, where possible, water always moves to the solution with the highest osmotic pressure (highest solute concentration).
  • Osmosis is the net movement of water through a selectively semipermeable membrane. Osmosis occurs only when the membrane is permeable to water but not to certain solutes.
  • The osmotic pressure that a solution exerts is proportional to the number of osmotically active particles in the solution.
  • The osmotic pressure of a solution is proportional to the concentration of the solute particles that cannot cross the membrane.

Tonicity

  • Tonicity is a measure of a solution's ability to change the volume of cells by altering their water contents.
  • In an isotonic solution, there is no net movement of water, so cells maintain their normal shape.
  • In a hypertonic solution, cells lose water and are in danger of shrinking or becoming dehydrated.
  • In a hypotonic solution, cells gain water and are in danger of swelling and bursting.
  • There are important medical uses of isotonic, hypertonic, and hypotonic solutions.
  • Tonicity can be demonstrated with red blood cells when placed in different saline solutions.
    • In an isotonic solution, they maintain their shape.
    • In a hypotonic solution, they undergo haemolysis (water floods the cell until the cell bursts).
    • In a hypertonic solution, they undergo crenation (water leaves the cell leading to dehydration and deformity).

Introduction to Glycolysis

  • Three major destinations for the nutrients we eat: Energy, structural or functional molecules, and storage compounds.
  • Most energy is derived from the oxidation of CHO, fat, and protein.
    • CHO
    • Fat
    • Protein
  • About 60-70% of the energy produced is lost as heat.
  • The remainder is stored as chemical energy (ATP).

Metabolic Reactions

  • Metabolism - all chemical reactions in the body
  • Catabolism - chemical reactions that break down complex organic molecules
  • Anabolism - chemical reactions that build up simple molecules into complex ones.
  • All molecules have energy stored between their bonds.
  • All chemical reactions depend on the transfer of a small amount of energy from one molecule to another.
  • This transfer is usually performed by ATP.

Adenosine Triphosphate (ATP)

  • A molecule for the temporary storage of energy.
  • Three phosphate groups attached to an adenine base and a 5c sugar (ribose).
  • ATP is used for:
    • Muscle contraction
    • Active transport
    • Movement of structures within a cell.
  • Large amounts of energy are released when the terminal phosphate bond is hydrolysed (broken).

Stages in Energy Generation

  • First stage: large molecules are broken into smaller units.
    • Proteins -- peptides and amino acids
    • Fats -- glycerol and fatty acids
    • Polysaccharides -- simple sugars.
  • Second stage: smaller units are degraded into a few key simple compounds that play a central role in metabolism.
  • Third stage: citric acid (Krebs cycle), oxidative phosphorylation.

Carbohydrate Metabolism

  • During digestion, polysaccharides and disaccharides are converted into monosaccharides (primarily glucose).
  • CHO metabolism is mostly concerned with glucose metabolism.
  • The oxidation of glucose is shown by the following reaction: C6H12O6 + 6O2 -> H2O + CO2 + 36ATP + heat
  • Glucose is catabolised in three different ways:
    • Glycolysis - Glucose -> pyruvic acid -> mitochondria -> processed through the Krebs cycle.
    • Krebs cycle
    • The electron transport chain and oxidative phosphorylation

Cellular Respiration

  • Metabolic pathways synthesise ATP.
    • Anaerobic - ATP production in the absence of O2 is glycolysis:
      • Glycolysis
      • Production of acetyl CoA as a transitional step.
    • Aerobic - ATP production using O2 is oxidative phosphorylation:
      • Krebs cycle
      • Electron transport chain.
  • Fuel + O2 -> CO2 + H2O + Energy (ATP + Heat)

Glycolysis

  • Overall equation: Glucose + 2ADP + 2Pi + 2NAD+ -> 2Pyruvate + 2ATP + 2NADH + 2H+ + 2H2O
  • Initial steps: Activate the glucose (2 phosphate groups); uses 2 molecules of ATP.
  • Later in glycolysis: 4 ATP (+ 2 NADH2) energy is liberated.
  • Energy Yield: {4 - 2} = 2 ATP + 2 NADH2 (you made 4 but required 2 to do it).
  • Although it doesn't seem like much, it's been made in the absence of O2 so it's 'budget friendly.'
  • Phase 1: Sugar activation: 2 ATP molecules are used to activate the glucose (fructose [type of sugar] - 1, 6 [referring to the carbons on the ring] -- bisphosphate [has phosphates on it]).
  • Phase 2: Sugar cleavage: 6C sugar is split into 2 x 3C sugars.
    • Each 3C sugar has a phosphate group.
    • Inorganic phosphate groups (Pi) are attached to each oxidised sugar fragment.
  • Phase 3: Oxidation and ATP formation:
    • The phosphates are split from the sugar and captured by ADP to form 4 ATP molecules.
    • The remaining 3C sugars are pyruvic acid.
    • The final products include 2 pyruvic acid molecules, 2NADH and H molecules (reduced NAD).
    • A net gain of 2 ATP molecules
    • If O2 is available, the pyruvic acid prepares to enter the Krebs cycle.
    • If O2 is not available, the pyruvic acid accepts H2 from NADH2 to form lactic acid (maintains supplies of NAD for glycolysis to continue).

Pyruvic Acid

  • The fate of pyruvic acid depends on the availability of O2.
  • When O2 is not available:
    • Pyruvic acid is reduced to lactic acid.
    • Lactic acid rapidly diffuses out of the cell and into the blood.
    • Liver cells remove lactic acid from the blood and convert it back to pyruvic acid.
  • When O2 is available:
    • Pyruvic acid proceeds to the Krebs cycle in the mitochondrion.

Formation of Acetyl Coenzyme A

  • Pyruvic acid enters the mitochondria and undergoes decarboxylation (remove CO2).
    • Pyruvate dehydrogenase converts 3C pyruvic acid to the compound 2C acetyl group plus CO2.
  • 2C acetyl group is attached to coenzyme A to form acetyl coenzyme A, which enters the Krebs cycle.
    • Coenzyme A is derived from vitamin B.
    • It behaves as a carrier/taxi for the 2C acetyl group.
  • The acetyl group is the active part of the molecule.
  • It is important to note that, despite the name, acetyl coenzyme A is not an acid in the strict chemical sense.

The Krebs Cycle

  • The Krebs cycle is also called the citric acid cycle or the tricarboxylic acid cycle.
  • It's a series of biochemical reactions that occur in the matrix of mitochondria.
    • Acetyl CoA (2C) enters the cycle and combines with a 4C compound to form citric acid.
  • The 2C component of acetyl CoA is pulled apart bit by bit to release CO2 and H+.
  • The H+ are sent to the electron transport chain (ETC) as NADH2 and FADH2 to be converted into energy to be converted into ATP.
  • Potential energy in the chemical bonds is released step by step to reduce the coenzymes (NAD+ -> NADH2, FAD+ -> FADH2) which temporarily store this energy.
  • NAD+ and FAD+ are the H2 carriers.
  • 2C Acetyl CoA + (4C) oxalo-acetic acid -> 6C (citric acid)
  • The series of reactions involving the removal of 2C and 4O as (in the form of) 2CO2 and the removal of hydrogen occurs.
  • 6C citric acid becomes 4C oxalo-acetic acid to complete the cyclic pathway.
  • Summary:
    • Each acetyl CoA molecule that enters the Krebs cycle produces:
      • 2 molecules of CO2
      • 3 molecules of NADH2
      • 1 molecule of ATP
      • 1 molecule of FADH2
    • Each glucose produces 2 acetyl CoA molecules.
    • Total yield = above products x 2.

Electron Transport Chain

  • The electron transport chain is located in the mitochondria.
  • Integral membrane proteins (cytochromes) form a chain located in the inner mitochondrial membrane.
  • Each cytochrome picks up electrons and passes them to the next in the chain.
  • Small amounts of energy are released as this occurs, using the energy to form ATP.
  • Oxidative phosphorylation produces the vast majority of ATP in the cell.

Summary of Aerobic Cellular Respiration

  • Glucose (+O2) is broken down into CO2 + H2O + Energy (ATP):
    • 2 ATP's are formed during glycolysis.
    • 2 NADH2 are formed during glycolysis.
    • 2 NADH2 are formed when converting pyruvate to acetyl CoA.
    • 2 ATP's are formed directly during the Krebs cycle.
    • 6 NADH2 are formed during the Krebs cycle.
    • 2 FADH2 are formed during the Krebs cycle.
  • For each NADH2, the proton gradient generates 3 ATP (10 NADH2 generates 10 x 3 ATP = 30 ATP).
  • For each FADH2, the proton gradient generates 2 ATP (2 FADH2 generates 2x2 ATP = 4 ATP)
  • From each glucose molecule, 4 ATP are created.
  • Oxidative phosphorylation generates 36-38 ATPs from one glucose molecule.
  • 2 x NADH2 formed during glycolysis produce less ATP via OP.
  • The complete oxidation of glucose can be represented as follows: C6H12O6 + 6O2 -> 6CO2 + 6H2O + 36-38 ATP.
  • Benefit: H2 obtained from a wide variety of organic molecules can be funneled through this process to form a common energy carrier -- ATP.
  • Involves a complex series of reactions in the mitochondrion.
  • Oxidative phosphorylation produces the vast majority of ATP in the cell.

Tutorial Experiment

  • The experiment is designed to test tonicity by observing the movement of water across a semi-permeable membrane (the bag).
  • Solution C is hypertonic to the solution inside the bag, causing water to move out of the bag into the cylinder, thus decreasing the volume of the bag and increasing the volume of the cylinder.
  • Solution A is hypotonic to the solution inside the bag, causing water to move into the bag from the cylinder, increasing the volume of the bag and decreasing the volume of the cylinder.
  • Solution B is isotonic to the solution inside the bag, resulting in no net movement of water, and thus no change in volume for either the bag or the cylinder.
  • The experiment shows the relationship between tonicity and the movement of water across a semi-permeable membrane.
  • The table provides data on the initial volume of the bag, the volume of the cylinder, and the final volume of both after the experiment.
  • The results show that the movement of water is determined by the tonicity of the solution in relation to the solution inside the bag.
  • The different tonicity solutions cause different changes in the volume of the bag and cylinder, illustrating the principles of osmosis.
  • The data in the table can be used to determine the relationship between tonicity and the movement of water molecules across a semi-permeable membrane.

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