Cell Biology: Membrane Structure

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Questions and Answers

What direction does water move during osmosis?

  • From hypertonic to isotonic
  • From hypotonic to hypertonic (correct)
  • From isotonic to hypertonic
  • From hypertonic to hypotonic

What happens to a cell placed in a hypotonic solution?

  • The cell swells and may burst (correct)
  • The cell remains unchanged in volume
  • The cell shrinks
  • The cell becomes shriveled

Which process allows large molecules to move out of a cell?

  • Endocytosis
  • Facilitated diffusion
  • Passive transport
  • Exocytosis (correct)

What characterizes an isotonic solution?

<p>Equal solute concentration as the cell (C)</p> Signup and view all the answers

What is a key characteristic of active transport?

<p>Requires energy to move substances against their concentration gradient (B)</p> Signup and view all the answers

In which part of the cell does glycolysis occur?

<p>Cytoplasm (B)</p> Signup and view all the answers

During which stage of cellular respiration is the electron transport chain located?

<p>Mitochondria (B)</p> Signup and view all the answers

What type of endocytosis involves the uptake of large particles?

<p>Phagocytosis (D)</p> Signup and view all the answers

What is the primary function of NADH and FADH2 in cellular respiration?

<p>They carry high energy electrons to the electron transport chain. (A)</p> Signup and view all the answers

Where in the cell does the electron transport chain take place?

<p>In the inner mitochondrial membrane (B)</p> Signup and view all the answers

What are the two main parts of photosynthesis?

<p>Light reactions and Calvin cycle (A)</p> Signup and view all the answers

What is the end product of the electron transport chain?

<p>Water (C)</p> Signup and view all the answers

Where is chlorophyll primarily located in plant cells?

<p>In thylakoid membranes (D)</p> Signup and view all the answers

Which protein complex is essential for ATP production during cellular respiration?

<p>ATP synthase (B)</p> Signup and view all the answers

What type of reactions occur in the electron transport chain?

<p>Redox reactions (C)</p> Signup and view all the answers

What is the function of pigment molecules in photosynthesis?

<p>To absorb and reflect certain wavelengths of light (A)</p> Signup and view all the answers

Which of the following substances can be used to generate ATP through cellular respiration?

<p>Carbohydrates, fats, and proteins (C)</p> Signup and view all the answers

What occurs when a pigment molecule absorbs a photon of light energy?

<p>The energy is transferred to an electron, exciting it (C)</p> Signup and view all the answers

What role does the enzyme play in the process of photosynthesis related to chlorophyll a?

<p>It splits water to replace lost electrons in chlorophyll a. (B)</p> Signup and view all the answers

Which photosystem absorbs light best at a wavelength of 700 nm?

<p>Photosystem I (C)</p> Signup and view all the answers

What is the regulatory mechanism that controls metabolic pathways like glycolysis?

<p>Feedback inhibition (C)</p> Signup and view all the answers

What happens to the chlorophyll a molecule that loses its high energy electron to the primary electron acceptor?

<p>It is oxidized and needs to be replaced (B)</p> Signup and view all the answers

What is the primary role of the electron transport chain connecting photosystem II to photosystem I?

<p>It generates a hydrogen ion gradient for ATP synthesis. (A)</p> Signup and view all the answers

What distinguishes autotrophs from heterotrophs?

<p>Autotrophs make organic molecules from carbon dioxide and water. (D)</p> Signup and view all the answers

What is the role of a spectrophotometer in photosynthesis studies?

<p>To measure the amount of light absorbed or transmitted (A)</p> Signup and view all the answers

What occurs to the electron from photosystem II after it passes through the electron transport chain?

<p>It replaces an activated electron in photosystem I. (D)</p> Signup and view all the answers

In the overall photosynthesis equation, where do the carbon atoms from carbon dioxide end up?

<p>As glucose in the products (A)</p> Signup and view all the answers

What is the product of the light reactions of photosynthesis?

<p>ATP and NADPH (D)</p> Signup and view all the answers

In what situation might the high energy electron from photosystem I be passed back to the electron transport chain instead of down to NADP+ reductase?

<p>When there is insufficient ATP being produced. (B)</p> Signup and view all the answers

Which of the following is NOT a phase of the Calvin cycle?

<p>Oxidation (D)</p> Signup and view all the answers

What molecule directly carries high energy electrons to the Calvin cycle?

<p>NADPH (C)</p> Signup and view all the answers

Which component is part of the electron transport chain connecting photosystem II to photosystem I?

<p>Plastoquinone (B)</p> Signup and view all the answers

What is the direct product of the Calvin cycle?

<p>Glyceraldehyde 3 phosphate (C)</p> Signup and view all the answers

What happens to the terminal phosphate group when ATP loses it?

<p>It is transferred to another molecule (A)</p> Signup and view all the answers

Which statement correctly describes oxidation in biochemical reactions?

<p>A molecule loses an electron (C)</p> Signup and view all the answers

What is the difference between catabolic and anabolic reactions?

<p>Catabolic reactions involve the breakdown of molecules while anabolic reactions build molecules (B)</p> Signup and view all the answers

What defines free energy in a system?

<p>Energy available to perform work at uniform temperature (C)</p> Signup and view all the answers

What characterizes exergonic reactions?

<p>They release energy as products have less free energy than reactants (B)</p> Signup and view all the answers

What is energy coupling in metabolic processes?

<p>Linking energy release from exergonic reactions to the energy requirements of endergonic reactions (D)</p> Signup and view all the answers

What is the central role of ATP in cellular energy needs?

<p>It provides short-term energy for cellular processes (C)</p> Signup and view all the answers

Which lipid is crucial for the structure of cell membranes due to its amphipathic nature?

<p>Phospholipid (A)</p> Signup and view all the answers

What is the main reason for membrane fluidity?

<p>Hydrophobic interactions between molecules (D)</p> Signup and view all the answers

What distinguishes integral proteins from peripheral proteins in cell membranes?

<p>Integral proteins are embedded within the membrane. (C)</p> Signup and view all the answers

What role do carbohydrates play in cell membranes?

<p>Cell-cell recognition (B)</p> Signup and view all the answers

How do hydrophilic substances typically cross cell membranes?

<p>Using protein channels and carriers (D)</p> Signup and view all the answers

What is selective permeability in the context of cell membranes?

<p>Hydrophobic molecules can pass, but polar molecules cannot. (C)</p> Signup and view all the answers

Which statement correctly describes the effect of cholesterol on membrane fluidity at body temperature?

<p>Cholesterol decreases fluidity. (B)</p> Signup and view all the answers

What characterizes active transport compared to passive transport?

<p>Active transport moves molecules from low concentration to high concentration. (C)</p> Signup and view all the answers

Flashcards

Passive Transport

Passive movement of molecules across a cell membrane from an area of high concentration to an area of low concentration, driven by the inherent kinetic energy of molecules, known as thermal motion.

Hypertonic Solution

A solution with a higher concentration of solutes compared to another solution.

Hypotonic Solution

A solution with a lower concentration of solutes compared to another solution.

Osmosis

The movement of water across a selectively permeable membrane from a region of higher water concentration to a region of lower water concentration.

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Vesicular Transport

The process by which cells transport large molecules across their membranes using vesicles.

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Phagocytosis

A type of vesicular transport where cells take in large particles, such as bacteria.

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Glycolysis

The first stage of cellular respiration, where glucose is broken down into pyruvate, producing ATP and NADH.

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Krebs Cycle

The second stage of cellular respiration, where pyruvate is oxidized in the mitochondria, producing ATP, NADH, and FADH2.

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What is Coenzyme A?

A derivative of a B vitamin that contains sulfur and is involved in various metabolic reactions, especially the breakdown of carbohydrates.

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What are the electron carriers formed during the Krebs cycle?

NADH and FADH2 carry high-energy electrons from glycolysis and the Krebs cycle to the electron transport chain.

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Photosynthesis

The process where plants capture sunlight and convert it into chemical energy in the form of glucose, releasing oxygen as a byproduct.

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Where is the electron transport chain located?

The inner mitochondrial membrane is the site of the electron transport chain.

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Photosystem

A cluster of light-absorbing pigments, proteins, and other organic molecules within the thylakoid membrane of chloroplasts.

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What type of reactions occur in the electron transport chain?

Reactions where electrons are transferred from one molecule to another. In the electron transport chain, electrons are passed from molecule to molecule, releasing energy.

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Light-gathering antenna

A group of chlorophyll and carotenoid pigment molecules that capture light energy and transfer it to a reaction center chlorophyll molecule.

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What is chemiosmosis?

The movement of protons across the inner mitochondrial membrane, driven by the electron transport chain, creating a concentration gradient that is used by ATP synthase to produce ATP.

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Light Reactions

The initial stage of photosynthesis, where light energy is converted to chemical energy.

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What is ATP synthase?

A complex of proteins in the inner mitochondrial membrane that uses the proton gradient created by the electron transport chain to synthesize ATP.

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Calvin Cycle

The second stage of photosynthesis, where carbon dioxide is used to synthesize sugars.

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What happens to electrons at the end of the electron transport chain?

Oxygen accepts the final electron in the Electron transport chain and combines with hydrogen ions to form water.

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What substances can be used in cellular respiration to generate ATP?

Proteins, carbohydrates, and fats can all be broken down and used in cellular respiration to generate ATP.

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Reaction Center Chlorophyll

The central chlorophyll molecule in a photosystem that transfers an excited electron to a primary electron acceptor.

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Primary Electron Acceptor

A substance that accepts an excited electron from the reaction center chlorophyll, initiating the electron transport chain.

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Pigment Absorption & Reflection

The process where pigment molecules absorb wavelengths of light and reflect others, resulting in the colors we perceive.

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What is the direct product of the Calvin cycle?

The main product of the Calvin cycle, a three-carbon sugar used to make glucose and other organic molecules.

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What is ATP?

The energy currency of the cell, used to power various cellular processes.

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What is a catabolic reaction?

The breakdown of complex molecules into simpler ones, releasing energy. Think of digestion.

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What is an anabolic reaction?

The synthesis of complex molecules from simpler ones, requiring energy input. Think of building a protein.

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What is energy?

The capacity to do work. It can exist in different forms, like kinetic and potential energy.

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What is kinetic energy?

The energy of motion. Think of a moving car.

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What is potential energy?

Energy that matter has due to its location or structure. Think of a ball held high up, ready to fall.

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What is free energy?

The portion of a system's energy that can be used to do work when temperature is uniform. Think of how much energy is actually usable.

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What is the basic structure of cell membranes?

The phospholipid bilayer, with its hydrophilic heads facing outward and hydrophobic tails facing inward, creates a selective barrier.

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What is meant by the 'fluid mosaic model' of cell membrane structure?

The arrangement of phospholipids and proteins allows for movement and flexibility, contributing to the membrane's fluid nature.

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What are the two main types of membrane proteins and how do they differ?

Integral proteins extend through the membrane and act as channels or carriers, allowing hydrophilic molecules to cross. Peripheral proteins are attached to the membrane surface, often aiding in cell signaling.

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What is meant by the 'selective permeability' of cell membranes?

Membranes allow small, hydrophobic molecules to pass through easily, but larger, hydrophilic molecules like ions and polar molecules require protein channels or carriers to cross.

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What is the key difference between active and passive transport?

Active transport moves substances against their concentration gradient, requiring energy from ATP. Passive transport moves substances down their concentration gradient, without requiring energy.

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What are the primary functions of membrane proteins?

Membrane proteins play a vital role in cellular function by facilitating transport, catalyzing chemical reactions, relaying signals, connecting cells, providing identification markers, and anchoring the cytoskeleton and extracellular matrix.

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How do membrane fluidity and composition relate?

Phospholipids with double bonds in their fatty acid tails increase membrane fluidity, making it easier for molecules to move around. Cholesterol molecules help to maintain membrane fluidity by preventing it from becoming too rigid or too fluid.

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What are oligosaccharides and why are they important for cell function?

Oligosaccharides are short chains of sugar molecules attached to lipids (glycolipids) or proteins (glycoproteins) on the cell surface, playing a critical role in cell-cell recognition and interactions.

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What happens to the lost electron in Photosystem II?

Photosystem II creates a high-energy electron by using light energy, losing its own electron in the process. To replace this electron, water is split, producing molecular oxygen (O2) as a byproduct.

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What happens to the high-energy electron generated by Photosystem II?

The energized electron from Photosystem II is passed down an electron transport chain to Photosystem I. This chain involves various components, including plastoquinone, cytochrome proteins, and plastocyanin. The energy released during this transfer helps generate a hydrogen ion gradient across the thylakoid membrane, which is later used by ATP synthase to produce ATP.

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What happens to the electron after the electron transport chain between Photosystem II and Photosystem I?

The electron that traveled through the electron transport chain from Photosystem II to Photosystem I arrives as low-energy. It is then utilized to replace the activated electron generated by Photosystem I.

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What happens to the high-energy electron generated by Photosystem I?

Photosystem I absorbs light energy, creating a high-energy electron. This electron is passed to the reaction center chlorophyll a molecule, then to the primary electron acceptor. It then continues down a different electron transport chain to ferredoxin. Ferredoxin then transfers it to the enzyme NADP+ reductase, which converts NADP+ to NADPH.

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Why is sometimes the high-energy electron from Photosystem I passed back to the cytochrome complex?

Sometimes, instead of passing the high-energy electron to NADP+ reductase, Photosystem I passes it to ferredoxin, sending it back to the cytochrome complex of the electron transport chain between Photosystem II and Photosystem I. This occurs because the Calvin cycle needs more ATP than NADPH.

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What molecules are used by the Calvin cycle and what does it generate?

The Calvin cycle utilizes ATP and NADPH produced during the light reactions. It uses carbon dioxide from the air to produce a three-carbon sugar molecule called glyceraldehyde 3-phosphate (G3P).

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What are the three phases of the Calvin cycle?

The Calvin cycle has three phases: Carbon fixation: CO2 is attached to a 5-carbon molecule called RuBP, which is catalyzed by the enzyme rubisco. Reduction: ATP is used to add phosphate groups, and NADPH is used to reduce the molecules, leading to G3P production. Regeneration: Some G3P is used to make sugars, and the rest regenerate RuBP.

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Study Notes

Membrane Structure

  • Three key molecules for cell membrane structure are lipids, proteins, and carbohydrates.
  • Phospholipids are a crucial type of lipid found in membranes.
  • Phospholipids are amphipathic, meaning they have both hydrophilic (water-loving) and hydrophobic (water-fearing) regions.
  • The current membrane model is the fluid-mosaic model. Membranes are fluid due to component movement and held together by hydrophobic interactions.
  • Factors affecting membrane fluidity include phospholipid double bonds and cholesterol content.
  • Membranes contain two main protein types: integral and peripheral proteins. Integral proteins are embedded within the membrane and often span it. Peripheral proteins are attached to the membrane's interior or exterior but are not embedded.
  • Membrane carbohydrates are important for cell-cell recognition.
  • Oligosaccharides, typically short chains, are attached to membrane lipids (glycolipids) and proteins (glycoproteins).

Membrane Transport

  • Selective permeability of membranes means hydrophobic molecules can pass through, but ions and polar molecules cannot without assistance.
  • Hydrophilic substances cross membranes using protein channels or carriers.
  • Active transport moves substances against concentration gradients using ATP. Passive transport moves substances from high to low concentration without energy.
  • Passive transport is driven by thermal motion.
  • Hypertonic solutions have higher solute concentrations, hypotonic solutions have lower solute concentrations, and isotonic solutions have equal solute concentrations.
  • Osmosis is the movement of water across a selectively permeable membrane, from a hypotonic to hypertonic solution.
  • Cells in fresh water may have contractile vacuoles, while plant cells have cell walls to maintain stable volume, reacting differently to isotonic, hypertonic, and hypotonic solutions.
  • Facilitated diffusion uses transport proteins to move substances passively, while active transport uses energy to move substances against concentration gradients.
  • Large molecules like polysaccharides and proteins cross membranes via vesicles (exocytosis and endocytosis).

Cellular Respiration

  • Cellular respiration occurs in three stages: glycolysis (cytoplasm), Krebs cycle (mitochondria), and electron transport chain (mitochondria).
  • Glycolysis starts with glucose and produces pyruvate, ATP, and NADH.
  • The Krebs cycle uses acetyl CoA and produces NADH, FADH2, and ATP.
  • The electron transport chain uses high-energy electrons from glycolysis and the Krebs cycle to generate ATP via chemiosmosis.
  • The final electron acceptor in the electron transport chain is oxygen. Water is a by-product of this process.

Photosynthesis

  • Photosynthesis is the process by which autotrophs convert carbon dioxide and water into glucose and oxygen using light energy.
  • Chlorophyll, located in thylakoid membranes within chloroplasts, captures light energy.
  • Photosynthesis has two main stages: light-dependent reactions (thylakoid membranes) and the Calvin cycle (stroma).
  • Light reactions convert light energy into chemical energy in the form of ATP and NADPH.
  • The Calvin cycle uses ATP and NADPH to convert carbon dioxide into glucose.
  • Photosynthesis involves the use of pigments that absorb specific wavelengths of light while reflecting others.

Energy Harvesting (ATP), Enzymes, Metabolism

  • ATP (adenosine triphosphate) is the central molecule for cellular energy needs.
  • ATP is important because its phosphate groups store energy. Losing the terminal phosphate releases energy.
  • Enzymes are proteins that act as catalysts, reducing the activation energy needed for chemical reactions.
  • Activation energy is the initial energy required to break chemical bonds in reactants.
  • Enzymes have active sites that bind substrates and lower activation energy, altering their shape to speed up the reaction.
  • Factors affecting enzyme activity include temperature, pH, and ionic concentrations.
  • Anabolic reactions build complex molecules, consuming energy. Catabolic reactions break complex molecules, releasing energy.

Additional Cellular Concepts / Metabolism

  • Co-factors are non-protein helpers (ions like zinc, copper, iron). Co-enzymes are organic molecules (vitamins or vitamin-derived).
  • Enzyme inhibitors can be reversible or irreversible and bind to the active site, affecting substrate binding or shape-altering.
  • Feedback inhibition is a common metabolic control mechanism where the product of a pathway inhibits the enzyme controlling an earlier reaction.
  • Enzyme cooperativity is when one substrate molecule facilitates the binding of additional substrate molecules to the enzyme.

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