Cell Biology: Intermediate Filaments and Junctions

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Questions and Answers

What is the main function of communicating junctions?

  • To prevent the movement of proteins between cells
  • To provide structural support to cells
  • To facilitate nutrient absorption
  • To permit movement of ions or signaling molecules between cells (correct)

What type of proteins form a seal in tight junctions?

  • Claudins and occludins (correct)
  • Selectins and desmogleins
  • Cadherins and integrins
  • Catenins and adherens

Which junction type has a Ca2+-dependent mechanism?

  • Tight junctions
  • Zonula adherentes (correct)
  • Desmosomes
  • Gap junctions

Which is a characteristic feature of desmosomes?

<p>Create small punctate attachments (B)</p> Signup and view all the answers

What structure is formed by the connection of two connexons?

<p>A gap junction channel (D)</p> Signup and view all the answers

What is the composition of the plaques in desmosomes?

<p>A series of attachment proteins (A)</p> Signup and view all the answers

What dimensions are gap junctions' intercellular channels typically characterized by?

<p>About 1.5 nm (A)</p> Signup and view all the answers

Which type of junction provides a mechanical connection between cells through intermediate filaments?

<p>Desmosomes (B)</p> Signup and view all the answers

What are the main structural components of intermediate filaments?

<p>They consist of tetramers of rod-like proteins. (B)</p> Signup and view all the answers

Which type of intermediate filament is primarily found in epithelial cells?

<p>Keratins (C)</p> Signup and view all the answers

What is the role of occluding junctions in cell biology?

<p>To join cells and form an impermeable barrier. (C)</p> Signup and view all the answers

What type of junctions are macula adherens?

<p>Spot-like junctions providing mechanical stability. (B)</p> Signup and view all the answers

Which statement is true regarding the individual subunits of intermediate filaments?

<p>The subunits vary considerably between different types. (B)</p> Signup and view all the answers

Which type of junction functions to maintain cell-to-cell or cell-to-basal lamina adherence?

<p>Anchoring junctions (D)</p> Signup and view all the answers

Which intermediate filament is associated with glial cells?

<p>Glial fibrillary acidic proteins (D)</p> Signup and view all the answers

What type of structure do fascia junctions create between cells?

<p>Broad areas of contact. (D)</p> Signup and view all the answers

Flashcards

Intermediate Filaments

Rope-like protein structures composed of tetramers, tightly bundled into helical arrays, providing structural support to cells. Different types exist, including keratins, desmin, vimentin, and more.

Keratins

A type of intermediate filament found in epithelial cells (skin, lining of organs).

Desmin

Intermediate filament found in muscle cells, providing stability to muscle tissue.

Intercellular Junctions

Specialized areas where cells connect, providing structural support and controlling the passage of materials between cells.

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Zonula (Belt-like) Junctions

A type of intercellular junction that encircles the cell like a belt.

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Anchoring Junctions

Cell junctions that provide mechanical stability to cells, holding cells together and to the extracellular matrix.

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Occluding Junctions

Cell junctions that form an impermeable barrier, preventing materials from passing between cells.

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Fascia (Sheet-like) Junctions

Intercellular junctions that form extensive sheets of contact between cells.

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Macula (Disc-like) Junctions

Intercellular junctions formed by spot-welds anchoring cells closely together.

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Tight Junctions

A type of communicating junction that forms a seal around cells, preventing the passage of molecules between them.

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Zonula adherens

A type of communicating junction that provides strong adhesion between cells, but allows substances to pass through.

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Claudins and Occludins

Transmembrane proteins that are crucial components of tight junctions, binding to each other to create a seal.

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Cadherins

Transmembrane linker proteins in zonula adherens that bind to each other via calcium ions.

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Desmosomes

Cell junctions that provide strong mechanical strength and resistance to stress, like spot welds.

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Gap Junctions

Cell junctions that allow the direct passage of small molecules and ions between cells.

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Intermediate Filaments

Cytoskeletal filaments that anchor desmosomes to the cell's internal structure.

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Connexins

Transmembrane proteins that form channels called connexons, permitting passage of small molecules between cells.

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Connexons

Structures formed by the assembly of connexin proteins, forming channels for passage of ions and small molecules between adjacent cells.

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Study Notes

Intermediate Filaments

  • Constructed from tetramers of rod-like proteins
  • Tightly bundled into helical arrays
  • Individual subunits vary based on filament type
  • Types include keratins, desmin, vimentin, glial fibrillary acidic protein, neurofilaments, and nuclear lamins

Intercellular Junctions

  • Specialized areas where cells connect
  • Classified morphologically into three types:
    • Zonula (belt-like) junctions: Encircle cells completely
    • Fascia (sheet-like) junctions: Form broad contact areas
    • Macula (disc-like) junctions: Like spot welds on cell surfaces
  • Functionally classified into types:
    • Occluding junctions: Form impermeable barriers
    • Anchoring junctions: Provide mechanical stability
    • Communicating junctions: Allow ion/molecule transfer

Cellular Junctions

  • Tight junctions (Zonula occludens):
    • Encircle cells
    • Formed by fusion of outer membrane layers
    • Seal prevents paracellular transport
    • Utilize claudins, occludins, and cadherins
  • Adherens junctions (Zonula adherens):
    • Located below tight junctions
    • Bind cells through cadherins
    • Link to actin filaments
  • Desmosomes (Macula adherens):
    • Spot welds connecting cells
    • Have plaques that bind intermediate filaments
    • Important for tissues under stress
  • Gap junctions (Nexus):
    • Specialized channels between cells
    • Made of connexons for ion/small molecule exchange

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