Cell Biology Chapter Overview

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Questions and Answers

What effect does a decreased surface area to volume (SA/V) ratio have on a cell's efficiency?

  • It makes nutrient and waste exchange less efficient. (correct)
  • It enhances nutrient uptake and waste disposal.
  • It decreases the need for the cell to enter the cell cycle.
  • It increases the rate of DNA replication.

During DNA replication, what role does primase play?

  • It brings RNA nucleotides complementary to the DNA template. (correct)
  • It unwinds the DNA double helix.
  • It synthesizes new DNA in a 3’ to 5’ direction.
  • It connects Okazaki fragments together.

What is the correct order of steps in the transcription process?

  • RNA polymerase movement, binding mRNA, RNA synthesis.
  • RNA synthesis, binding to promoter, RNA polymerase movement.
  • Initiation, elongation, termination.
  • Binding to promoter, RNA synthesis, RNA polymerase movement. (correct)

What is the role of tRNA in translation?

<p>To bring amino acids to the ribosome. (C)</p> Signup and view all the answers

What is the significance of the stop codon in the translation process?

<p>It halts the translation process. (B)</p> Signup and view all the answers

What does the leading strand do during DNA replication?

<p>It is synthesized continuously toward the replication fork. (B)</p> Signup and view all the answers

Which component of the ribosome plays a critical role in linking amino acids during protein synthesis?

<p>rRNA (D)</p> Signup and view all the answers

Which phase of the cell cycle is primarily associated with DNA replication?

<p>S phase (D)</p> Signup and view all the answers

What initiates the transcription process at the promoter region?

<p>RNA polymerase (C)</p> Signup and view all the answers

What is the first amino acid added to a growing polypeptide chain during translation?

<p>Methionine (D)</p> Signup and view all the answers

Flashcards

SA/V Ratio

The ratio of a cell's surface area to its volume. It's important because it affects how efficiently nutrients can enter and waste can exit a cell.

Cell Cycle

The process where a larger cell divides into two smaller cells. It's triggered when the SA/V ratio gets too small, making it hard for the cell to function efficiently.

Primase

An enzyme crucial for DNA replication. It helps in creating short RNA sequences (primers) that act as starting points for DNA polymerase to begin copying DNA.

Leading Strand

The newly synthesized DNA strand that's made continuously during replication. It's made in the 5' to 3' direction, following DNA polymerase.

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Okazaki Fragments

Small DNA fragments created on the lagging strand during replication. They are synthesized in the 5' to 3' direction but in short pieces.

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Lagging Strand

The newly synthesized DNA strand that's made discontinuously during replication. It's made in the 5' to 3' direction, but against the direction of the replication fork.

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Translation

The process of converting RNA into proteins. It uses the genetic code from mRNA to assemble amino acids into a specific protein sequence.

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tRNA

A type of RNA that carries specific amino acids to the ribosome during translation. It acts as a bridge between mRNA's code and the protein sequence.

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rRNA

A type of RNA that forms the structural core of ribosomes. It plays a crucial role in linking amino acids together during protein synthesis.

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mRNA

A type of RNA that carries a copy of the gene's genetic message from the nucleus to the ribosome for protein synthesis.

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Study Notes

Surface Area to Volume Ratio (SA/V) and Cell Cycle

  • A decreasing SA/V ratio in a cell makes nutrient and waste transport less efficient due to lower surface area relative to volume.
  • A small SA/V ratio in large cells triggers signals for cell division.
  • Conversely, an increasing SA/V ratio (smaller cells) simplifies nutrient/waste management.

DNA Replication

  • Primase: An enzyme crucial in DNA replication, adding RNA nucleotides complementary to the DNA template.
  • Leading Strand: Synthesized continuously from the 5' to 3' direction on the template strand.
  • Okazaki Fragments: Short DNA segments formed discontinuously on the lagging strand, synthesised from the 5' to 3' direction in separate sections.
  • Lagging Strand: New DNA synthesized discontinuously in short segments opposite the replication fork.

Translation (RNA to Protein)

  • tRNA (Transfer RNA): Carries amino acids to ribosomes for protein synthesis.
  • rRNA (Ribosomal RNA): Forms ribosomes, aiding in protein synthesis and amino acid linking.
  • mRNA (Messenger RNA): Carries the genetic code from DNA to ribosomes for protein synthesis.
  • Methionine: The initial amino acid in protein synthesis.
  • Translation Steps:
    • mRNA binds to the small ribosomal subunit.
    • Ribosome formation and subsequent tRNA entry.
    • Peptide bond formation.
    • Translocation (ribosome movement along mRNA).
    • Stop codon signals translation termination.

Transcription (DNA to RNA)

  • Promoter: DNA region where transcription begins.
  • Transcription Steps:
    • RNA polymerase constructs RNA.
    • RNA polymerase moves along the DNA in a 3' to 5' direction, producing a 3' to 5' RNA molecule.

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