Cell Biology and Microscopy Quiz

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Questions and Answers

What is the purpose of using a fixative like glutaraldehyde on tissue samples?

  • To preserve the cells within the tissue (correct)
  • To make the samples more opaque
  • To dissolve the tissue for easier examination
  • To enhance the color of the tissue samples

What is fluorescence microscopy primarily used for?

  • To observe cellular movement in real-time
  • To analyze the genetic material in cells
  • To create three-dimensional images of tissues
  • To detect specific proteins or other molecules (correct)

How does a confocal microscope differ from a conventional light microscope?

  • It focuses light on a single point at specific depths (correct)
  • It cannot produce three-dimensional images
  • It requires special staining techniques
  • It uses multiple light sources

What is the role of the filters in a fluorescence microscope?

<p>To filter specific wavelengths of light (A)</p> Signup and view all the answers

What is the typical resolution limit of an electron microscope?

<p>0.002 nm (A)</p> Signup and view all the answers

What happens to the wavelength of an electron as its velocity increases?

<p>It decreases (A)</p> Signup and view all the answers

Why are tissue samples often cut into thin sections?

<p>To facilitate high-resolution examination (C)</p> Signup and view all the answers

In fluorescence microscopy, what does the first filter do?

<p>It passes only the wavelengths that excite the specific dye (D)</p> Signup and view all the answers

What type of bonds holds the two strands of DNA together?

<p>Hydrogen bonds (A)</p> Signup and view all the answers

Which term describes a DNA fragment coding for a protein or non-coding RNA?

<p>Gene (D)</p> Signup and view all the answers

What is the first process that utilizes DNA to synthesize RNA?

<p>Transcription (A)</p> Signup and view all the answers

What structure encloses each cell and acts as a selective barrier?

<p>Plasma membrane (C)</p> Signup and view all the answers

What are the molecules that make up the bilayer of the plasma membrane?

<p>Phospholipids (D)</p> Signup and view all the answers

What process involves the synthesis of proteins directed by mRNAs?

<p>Translation (A)</p> Signup and view all the answers

Which of the following domains of life has a nucleus?

<p>Eukaryotes (A)</p> Signup and view all the answers

What can mutations lead to during DNA replication?

<p>Genetic diversity (B)</p> Signup and view all the answers

What is the layer formed by amphiphilic components in water?

<p>Bilayer (A)</p> Signup and view all the answers

Which structure is NOT found in prokaryotes?

<p>Nucleus (A)</p> Signup and view all the answers

What is the primary purpose of the filament in the electron microscope?

<p>To emit electrons (A)</p> Signup and view all the answers

Why must the air be pumped out of the column in the electron microscope?

<p>To create a vacuum for the electron beam (D)</p> Signup and view all the answers

What is the usual thickness of the ultrathin sections of fixed tissue viewed in an electron microscope?

<p>25–100 nm thick (B)</p> Signup and view all the answers

What role do the magnetic coils play in the operation of the electron microscope?

<p>They focus the electron beam (B)</p> Signup and view all the answers

What process is often used to preserve living tissue before examining it with an electron microscope?

<p>Chemical fixation (A)</p> Signup and view all the answers

What must be done to the specimen before it can be viewed in the electron microscope?

<p>It must be cut into thin sections (A)</p> Signup and view all the answers

What kind of knife is used to cut the specimens in an electron microscope?

<p>A fine glass or diamond knife (A)</p> Signup and view all the answers

What happens to living tissue when it is exposed to the vacuum in an electron microscope?

<p>It is killed and preserved by chemical fixation (C)</p> Signup and view all the answers

How is the specimen prepared after dehydration for the electron microscope?

<p>It is permeated with a resin (A)</p> Signup and view all the answers

What is the purpose of the small metal grid mentioned in the context of an electron microscope?

<p>To support the specimen during viewing (B)</p> Signup and view all the answers

What is the primary function of the regulatory DNA in an organism's genome?

<p>To control gene expression (A)</p> Signup and view all the answers

Which of the following correctly describes bacteriophages?

<p>They are types of viruses that infect bacteria (A)</p> Signup and view all the answers

What are the two main approaches to analyzing gene function?

<p>Biochemistry and Genetics (B)</p> Signup and view all the answers

Which statement about model organisms is true?

<p>They aid in analyzing gene function through simplified systems (B)</p> Signup and view all the answers

How do viruses typically replicate their genetic information?

<p>By using enzymes found in the host cell (C)</p> Signup and view all the answers

What characterizes the genome of coronaviruses?

<p>It consists of large single-stranded RNA molecules (B)</p> Signup and view all the answers

What is the primary component of the hereditary information stored in cells?

<p>Double-stranded DNA (B)</p> Signup and view all the answers

Which part of a DNA monomer provides structural framework?

<p>Phosphate group (A), Sugar (D)</p> Signup and view all the answers

What is a notable feature of bacteria as model organisms?

<p>They duplicate every 20 minutes under optimal conditions (B)</p> Signup and view all the answers

In what form do all cells on Earth store their hereditary information?

<p>Double-strand molecules of DNA (D)</p> Signup and view all the answers

What is the role of enzymes in biological systems?

<p>They serve as biological catalysts. (A)</p> Signup and view all the answers

Which of the following cellular components is primarily linked to intercellular communication?

<p>Cell membranes (A)</p> Signup and view all the answers

Which process is involved in the synthesis of RNA from a DNA template?

<p>Transcription (A)</p> Signup and view all the answers

What structural form do most living organisms primarily consist of?

<p>Single cells (A)</p> Signup and view all the answers

What is necessary for the flow of genetic information to occur?

<p>DNA and RNA (B)</p> Signup and view all the answers

Which nucleotide bases are found in DNA?

<p>A, G, C, T (D)</p> Signup and view all the answers

Which polymerase is involved in the transcription process in eukaryotes?

<p>RNA polymerase (C)</p> Signup and view all the answers

Flashcards

What are tissue sections?

Tissue samples are often cut into thin, transparent slices called sections to allow for microscopic examination.

What is a fixative used for in tissue preparation?

Glutaraldehyde is a commonly used chemical that preserves the cells in a tissue sample, preventing them from degrading.

How are tissues sometimes prepared for sectioning?

Tissues are sometimes frozen in a specific medium, which later solidifies into a block that can be easily sliced.

What is the purpose of staining tissue sections?

Organic dyes with an affinity for certain cell structures are used to stain the sections, making specific components visible.

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How does fluorescence microscopy work?

Fluorescence microscopy uses fluorescent dyes that emit light when excited by specific wavelengths.

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What is a confocal microscope?

The confocal microscope is specially designed for fluorescence microscopy, allowing it to focus on single points in the specimen.

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What is the principle behind electron microscopy?

Electrons are used instead of light in electron microscopy, providing much higher resolution and allowing viewing of smaller structures.

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What is the advantage of an electron microscope?

The resolution of an electron microscope is significantly higher than a light microscope due to the shorter wavelength of electrons.

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Filament or cathode

A source of electrons that are used to illuminate the specimen in an electron microscope.

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Vacuum creation

A process where air is removed from the electron microscope's column to create a vacuum.

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Electron acceleration

The process of accelerating electrons from the filament towards the specimen.

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Electron beam

A focused beam of electrons used to illuminate the specimen in an electron microscope.

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Magnetic coils

Coils placed along the column of the electron microscope that focus the electron beam.

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Specimen preparation

The process of preparing a specimen for electron microscopy by removing water and replacing it with a resin.

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Microtome

A device that cuts extremely thin slices of a specimen for electron microscopy.

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Ultrathin sections

Extremely thin slices of a specimen, about 1/200 the thickness of a single cell, used in electron microscopy.

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Chemical fixation

The process of killing and preserving living tissue for electron microscopy.

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Metal grid

A small metal grid used to support ultrathin sections for viewing in an electron microscope.

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What is a cell?

The basic unit of life, capable of independent existence and carrying out all essential life functions.

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What information is stored within a cell?

A single cell contains all the hereditary information that defines a species. This information is passed down through generations.

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How is genetic information stored within a cell?

DNA, a double-stranded molecule composed of nucleotides, carries the genetic instructions for building and maintaining an organism.

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What are the components of DNA?

Each nucleotide in DNA consists of a sugar (deoxyribose), a phosphate group, and a base (A, G, C, T).

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How does DNA determine the characteristics of an organism?

The order of bases (A, G, C, T) in DNA determines the genetic code, which instructs the cell to produce specific proteins.

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What is a tissue?

A group of cells that work together to perform a specialized function in an organism.

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What is cell division?

The process by which a cell divides to produce two identical daughter cells, ensuring the continuity of genetic information.

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Where is DNA located in eukaryotic cells?

In eukaryotic cells, the genetic material is organized into structures called chromosomes, which contain DNA tightly packaged with proteins.

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What is DNA replication?

The process by which DNA is copied to produce two identical DNA molecules, ensuring that each daughter cell gets a complete set of genetic information.

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What is transcription?

The process by which DNA is transcribed into RNA, a molecule that carries genetic information from DNA to ribosomes, the protein synthesis machinery.

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DNA

A double-stranded molecule composed of nucleotides, it carries genetic information in the form of a sequence of bases: Adenine (A), Thymine (T), Guanine (G), and Cytosine (C).

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DNA replication

The process by which a cell creates two identical copies of its DNA before cell division, ensuring each daughter cell receives a complete genome.

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Gene

A segment of DNA that codes for a specific protein or RNA molecule.

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Transcription

The process by which DNA's genetic information is copied into a messenger RNA (mRNA) molecule.

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Translation

The process by which mRNA's instructions are decoded to assemble a chain of amino acids, forming a protein.

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mRNA (messenger RNA)

A type of RNA that carries the genetic information from DNA to ribosomes, where proteins are made.

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rRNA (ribosomal RNA)

A type of RNA that forms part of the ribosome, the cellular machinery for protein synthesis.

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Plasma membrane

A cell's outer boundary, acting as a selectively permeable barrier. Its structure is based on a phospholipid bilayer, which arranges in a specific way due to the properties of its molecules.

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Phospholipid

The building block of cell membranes, with a hydrophilic head and hydrophobic tails, forming a bilayer structure in water.

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Mutations

Changes in the DNA sequence that can lead to variations in traits. Some mutations are harmful, some neutral, and some beneficial.

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What are viruses?

These are packets of genetic material that rely on a host cell to reproduce.

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What are bacteriophages?

Bacteriophages are viruses that infect bacteria. They can be used as vectors to transfer genes between cells.

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Why are bacteria ideal model organisms?

Bacteria are single-celled organisms known for their rapid reproduction and adaptability. They are widely studied in laboratories.

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Why is yeast a good model organism?

Yeast is a single-celled organism that exhibits both sexual and asexual reproduction, making it convenient for researching eukaryotic cells.

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What are model organisms?

Model organisms are organisms chosen for research due to their suitability for studying specific biological processes.

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How can cells with identical genomes have diverse functions?

The selective expression of genes based on a cell's developmental history and external cues leads to diverse cell types while maintaining the same genome.

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How do we understand gene function?

Gene function is analyzed by two complementary approaches: studying purified molecules (biochemistry) and examining altered genes (genetics).

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Study Notes

Course Information

  • Course: Biology and Genetics I
  • Academic Year: 2024-2025
  • Instructor: Prof. Cecilia Battistelli
  • Email: [email protected]

Course Content

  • Introduction to cellular biology: diversity and similarity of living organisms, Biology and the scientific method, Origin and Evolution of Cells, Cells as experimental models, Tools of Cell Biology, The Chemistry of Life (molecular composition of cells), Enzymes as biological catalysts
  • The flow of genetic information: Nucleic acids, Chromatin and Chromosomes, DNA Replication, DNA Repair, RNA Synthesis and Processing (transcription in prokaryotes, eukaryotic RNA polymerases, general transcription factors, regulation of transcription in eukaryotes, RNA processing and turnover), Protein Synthesis, Processing, and Regulation (translation of mRNA, protein folding and processing, regulation of protein function, protein degradation)
  • Human genetics and techniques
  • Cell and Molecular Biology (Gerald Karp, Wiley & Sons, Inc)
  • World of the Cell (Becker, Kleinsmith, Hardin, Bertoni, Pearson Education)
  • The Cell, a molecular approach (Geoffrey M Cooper, Sinauer)
  • Molecular Biology of the Cell (Bruce Alberts, NCBI bookshelf online)

Exam Information

  • Written test for both biology I and genetics I, on either 12/02/2025 or 26/2/2025
  • Registration via GOMP website

Lecture Schedule

  • Lectures from October 7, 2024 to January 30, 2025
  • Time: 14:00 to 16:00

Additional Topics (from subsequent pages)

  • Cells, Genomes, and the Diversity of Life
  • Most living organisms are single cells. Others form groups linked by intercellular communication, generated from a single cell.
  • All cells store hereditary information as double-stranded DNA. DNA strands consist of sugars, phosphates, and bases (A, T, C, G).
  • DNA replication uses one strand as a template for a complementary strand.
  • DNA expresses information by guiding the synthesis of other molecules (genes for proteins/non-coding RNA). RNA synthesis (transcription) followed by protein synthesis (translation).
  • Proteins act in various cellular functions, including catalysis(speeding up chemical reactions without changing themselves). They maintain hereditary information.
  • Cells have plasma membranes that act as selective barriers for molecules. Plasma membranes are formed of amphiphilic phospholipids.
  • All membranes contain membrane transport proteins.
  • Differences in DNA sequence provide quantitative measures of evolutionary distance. The living world encompasses three domains: Eukaryotes, Bacteria, and Archaea.
  • Eukaryotes have a nucleus, Bacteria and Archaea do not. Bacteria can be spherical, rod-shaped, or spiral.
  • Mutations occur during DNA replication and repair, including gene duplication, break and rejoin of DNA segments, and transfer of DNA between cells.
  • Genes derived from a common ancestor are orthologs; duplicated genes that diverge functionally are paralogs.
  • Many genes are common to all three domains of life, involved in roles such as information processing and metabolic activities.
  • Eukaryotic cells are complex, containing organelles and a cytoskeleton.
  • Eukaryotic cells may employ endocytosis (import) and exocytosis (export) to mediate material exchange between organelles.
  • Other organelles, like mitochondria and chloroplasts, are enclosed in a double membrane. Mitochondria produce ATP from food molecules. They are similar in structure to bacteria.
  • Eukaryotic cells evolved through endosymbiosis between an anaerobic archaeon and an aerobic bacterium.
  • Some prokaryotic genes transitioned to eukaryotic nuclei.
  • Eukaryotic DNA is large, with significant non-coding regions that control gene expression,
  • Model organisms are used in gene function analyses (combined biochemistry and genetics approaches, using mutants).
  • Viruses, including bacteriophages that infect bacteria, use diverse methods of gene transfer.
  • Bacteria can adapt to variable conditions via mutation and selection.
  • Yeast (a eukaryotic organism), are easy to grow in simple medium, capable of both sexual and asexual reproduction.
  • Variety of model organisms used in biological research and studies.
  • Visualizing Cells and Their Molecules (using light microscopy variations like brightfield, darkfield, phase-contrast, and electron microscopy—scanning/transmission)
  • Techniques to visualize cell structures include staining, sectioning (for thick samples), and using fluorescent dyes. Techniques to isolate and separate proteins include column chromatography, polyacrylamide-gel electrophoresis, and mass spectrometry.
  • Analysis of large, complex macromolecules like DNA and proteins relies on techniques like x-ray crystallography and cryo-electron microscopy.

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