Podcast
Questions and Answers
What is the purpose of using a fixative like glutaraldehyde on tissue samples?
What is the purpose of using a fixative like glutaraldehyde on tissue samples?
- To preserve the cells within the tissue (correct)
- To make the samples more opaque
- To dissolve the tissue for easier examination
- To enhance the color of the tissue samples
What is fluorescence microscopy primarily used for?
What is fluorescence microscopy primarily used for?
- To observe cellular movement in real-time
- To analyze the genetic material in cells
- To create three-dimensional images of tissues
- To detect specific proteins or other molecules (correct)
How does a confocal microscope differ from a conventional light microscope?
How does a confocal microscope differ from a conventional light microscope?
- It focuses light on a single point at specific depths (correct)
- It cannot produce three-dimensional images
- It requires special staining techniques
- It uses multiple light sources
What is the role of the filters in a fluorescence microscope?
What is the role of the filters in a fluorescence microscope?
What is the typical resolution limit of an electron microscope?
What is the typical resolution limit of an electron microscope?
What happens to the wavelength of an electron as its velocity increases?
What happens to the wavelength of an electron as its velocity increases?
Why are tissue samples often cut into thin sections?
Why are tissue samples often cut into thin sections?
In fluorescence microscopy, what does the first filter do?
In fluorescence microscopy, what does the first filter do?
What type of bonds holds the two strands of DNA together?
What type of bonds holds the two strands of DNA together?
Which term describes a DNA fragment coding for a protein or non-coding RNA?
Which term describes a DNA fragment coding for a protein or non-coding RNA?
What is the first process that utilizes DNA to synthesize RNA?
What is the first process that utilizes DNA to synthesize RNA?
What structure encloses each cell and acts as a selective barrier?
What structure encloses each cell and acts as a selective barrier?
What are the molecules that make up the bilayer of the plasma membrane?
What are the molecules that make up the bilayer of the plasma membrane?
What process involves the synthesis of proteins directed by mRNAs?
What process involves the synthesis of proteins directed by mRNAs?
Which of the following domains of life has a nucleus?
Which of the following domains of life has a nucleus?
What can mutations lead to during DNA replication?
What can mutations lead to during DNA replication?
What is the layer formed by amphiphilic components in water?
What is the layer formed by amphiphilic components in water?
Which structure is NOT found in prokaryotes?
Which structure is NOT found in prokaryotes?
What is the primary purpose of the filament in the electron microscope?
What is the primary purpose of the filament in the electron microscope?
Why must the air be pumped out of the column in the electron microscope?
Why must the air be pumped out of the column in the electron microscope?
What is the usual thickness of the ultrathin sections of fixed tissue viewed in an electron microscope?
What is the usual thickness of the ultrathin sections of fixed tissue viewed in an electron microscope?
What role do the magnetic coils play in the operation of the electron microscope?
What role do the magnetic coils play in the operation of the electron microscope?
What process is often used to preserve living tissue before examining it with an electron microscope?
What process is often used to preserve living tissue before examining it with an electron microscope?
What must be done to the specimen before it can be viewed in the electron microscope?
What must be done to the specimen before it can be viewed in the electron microscope?
What kind of knife is used to cut the specimens in an electron microscope?
What kind of knife is used to cut the specimens in an electron microscope?
What happens to living tissue when it is exposed to the vacuum in an electron microscope?
What happens to living tissue when it is exposed to the vacuum in an electron microscope?
How is the specimen prepared after dehydration for the electron microscope?
How is the specimen prepared after dehydration for the electron microscope?
What is the purpose of the small metal grid mentioned in the context of an electron microscope?
What is the purpose of the small metal grid mentioned in the context of an electron microscope?
What is the primary function of the regulatory DNA in an organism's genome?
What is the primary function of the regulatory DNA in an organism's genome?
Which of the following correctly describes bacteriophages?
Which of the following correctly describes bacteriophages?
What are the two main approaches to analyzing gene function?
What are the two main approaches to analyzing gene function?
Which statement about model organisms is true?
Which statement about model organisms is true?
How do viruses typically replicate their genetic information?
How do viruses typically replicate their genetic information?
What characterizes the genome of coronaviruses?
What characterizes the genome of coronaviruses?
What is the primary component of the hereditary information stored in cells?
What is the primary component of the hereditary information stored in cells?
Which part of a DNA monomer provides structural framework?
Which part of a DNA monomer provides structural framework?
What is a notable feature of bacteria as model organisms?
What is a notable feature of bacteria as model organisms?
In what form do all cells on Earth store their hereditary information?
In what form do all cells on Earth store their hereditary information?
What is the role of enzymes in biological systems?
What is the role of enzymes in biological systems?
Which of the following cellular components is primarily linked to intercellular communication?
Which of the following cellular components is primarily linked to intercellular communication?
Which process is involved in the synthesis of RNA from a DNA template?
Which process is involved in the synthesis of RNA from a DNA template?
What structural form do most living organisms primarily consist of?
What structural form do most living organisms primarily consist of?
What is necessary for the flow of genetic information to occur?
What is necessary for the flow of genetic information to occur?
Which nucleotide bases are found in DNA?
Which nucleotide bases are found in DNA?
Which polymerase is involved in the transcription process in eukaryotes?
Which polymerase is involved in the transcription process in eukaryotes?
Flashcards
What are tissue sections?
What are tissue sections?
Tissue samples are often cut into thin, transparent slices called sections to allow for microscopic examination.
What is a fixative used for in tissue preparation?
What is a fixative used for in tissue preparation?
Glutaraldehyde is a commonly used chemical that preserves the cells in a tissue sample, preventing them from degrading.
How are tissues sometimes prepared for sectioning?
How are tissues sometimes prepared for sectioning?
Tissues are sometimes frozen in a specific medium, which later solidifies into a block that can be easily sliced.
What is the purpose of staining tissue sections?
What is the purpose of staining tissue sections?
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How does fluorescence microscopy work?
How does fluorescence microscopy work?
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What is a confocal microscope?
What is a confocal microscope?
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What is the principle behind electron microscopy?
What is the principle behind electron microscopy?
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What is the advantage of an electron microscope?
What is the advantage of an electron microscope?
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Filament or cathode
Filament or cathode
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Vacuum creation
Vacuum creation
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Electron acceleration
Electron acceleration
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Electron beam
Electron beam
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Magnetic coils
Magnetic coils
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Specimen preparation
Specimen preparation
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Microtome
Microtome
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Ultrathin sections
Ultrathin sections
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Chemical fixation
Chemical fixation
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Metal grid
Metal grid
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What is a cell?
What is a cell?
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What information is stored within a cell?
What information is stored within a cell?
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How is genetic information stored within a cell?
How is genetic information stored within a cell?
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What are the components of DNA?
What are the components of DNA?
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How does DNA determine the characteristics of an organism?
How does DNA determine the characteristics of an organism?
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What is a tissue?
What is a tissue?
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What is cell division?
What is cell division?
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Where is DNA located in eukaryotic cells?
Where is DNA located in eukaryotic cells?
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What is DNA replication?
What is DNA replication?
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What is transcription?
What is transcription?
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DNA
DNA
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DNA replication
DNA replication
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Gene
Gene
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Transcription
Transcription
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Translation
Translation
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mRNA (messenger RNA)
mRNA (messenger RNA)
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rRNA (ribosomal RNA)
rRNA (ribosomal RNA)
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Plasma membrane
Plasma membrane
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Phospholipid
Phospholipid
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Mutations
Mutations
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What are viruses?
What are viruses?
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What are bacteriophages?
What are bacteriophages?
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Why are bacteria ideal model organisms?
Why are bacteria ideal model organisms?
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Why is yeast a good model organism?
Why is yeast a good model organism?
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What are model organisms?
What are model organisms?
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How can cells with identical genomes have diverse functions?
How can cells with identical genomes have diverse functions?
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How do we understand gene function?
How do we understand gene function?
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Study Notes
Course Information
- Course: Biology and Genetics I
- Academic Year: 2024-2025
- Instructor: Prof. Cecilia Battistelli
- Email: [email protected]
Course Content
- Introduction to cellular biology: diversity and similarity of living organisms, Biology and the scientific method, Origin and Evolution of Cells, Cells as experimental models, Tools of Cell Biology, The Chemistry of Life (molecular composition of cells), Enzymes as biological catalysts
- The flow of genetic information: Nucleic acids, Chromatin and Chromosomes, DNA Replication, DNA Repair, RNA Synthesis and Processing (transcription in prokaryotes, eukaryotic RNA polymerases, general transcription factors, regulation of transcription in eukaryotes, RNA processing and turnover), Protein Synthesis, Processing, and Regulation (translation of mRNA, protein folding and processing, regulation of protein function, protein degradation)
- Human genetics and techniques
Recommended Texts
- Cell and Molecular Biology (Gerald Karp, Wiley & Sons, Inc)
- World of the Cell (Becker, Kleinsmith, Hardin, Bertoni, Pearson Education)
- The Cell, a molecular approach (Geoffrey M Cooper, Sinauer)
- Molecular Biology of the Cell (Bruce Alberts, NCBI bookshelf online)
Exam Information
- Written test for both biology I and genetics I, on either 12/02/2025 or 26/2/2025
- Registration via GOMP website
Lecture Schedule
- Lectures from October 7, 2024 to January 30, 2025
- Time: 14:00 to 16:00
Additional Topics (from subsequent pages)
- Cells, Genomes, and the Diversity of Life
- Most living organisms are single cells. Others form groups linked by intercellular communication, generated from a single cell.
- All cells store hereditary information as double-stranded DNA. DNA strands consist of sugars, phosphates, and bases (A, T, C, G).
- DNA replication uses one strand as a template for a complementary strand.
- DNA expresses information by guiding the synthesis of other molecules (genes for proteins/non-coding RNA). RNA synthesis (transcription) followed by protein synthesis (translation).
- Proteins act in various cellular functions, including catalysis(speeding up chemical reactions without changing themselves). They maintain hereditary information.
- Cells have plasma membranes that act as selective barriers for molecules. Plasma membranes are formed of amphiphilic phospholipids.
- All membranes contain membrane transport proteins.
- Differences in DNA sequence provide quantitative measures of evolutionary distance. The living world encompasses three domains: Eukaryotes, Bacteria, and Archaea.
- Eukaryotes have a nucleus, Bacteria and Archaea do not. Bacteria can be spherical, rod-shaped, or spiral.
- Mutations occur during DNA replication and repair, including gene duplication, break and rejoin of DNA segments, and transfer of DNA between cells.
- Genes derived from a common ancestor are orthologs; duplicated genes that diverge functionally are paralogs.
- Many genes are common to all three domains of life, involved in roles such as information processing and metabolic activities.
- Eukaryotic cells are complex, containing organelles and a cytoskeleton.
- Eukaryotic cells may employ endocytosis (import) and exocytosis (export) to mediate material exchange between organelles.
- Other organelles, like mitochondria and chloroplasts, are enclosed in a double membrane. Mitochondria produce ATP from food molecules. They are similar in structure to bacteria.
- Eukaryotic cells evolved through endosymbiosis between an anaerobic archaeon and an aerobic bacterium.
- Some prokaryotic genes transitioned to eukaryotic nuclei.
- Eukaryotic DNA is large, with significant non-coding regions that control gene expression,
- Model organisms are used in gene function analyses (combined biochemistry and genetics approaches, using mutants).
- Viruses, including bacteriophages that infect bacteria, use diverse methods of gene transfer.
- Bacteria can adapt to variable conditions via mutation and selection.
- Yeast (a eukaryotic organism), are easy to grow in simple medium, capable of both sexual and asexual reproduction.
- Variety of model organisms used in biological research and studies.
- Visualizing Cells and Their Molecules (using light microscopy variations like brightfield, darkfield, phase-contrast, and electron microscopy—scanning/transmission)
- Techniques to visualize cell structures include staining, sectioning (for thick samples), and using fluorescent dyes. Techniques to isolate and separate proteins include column chromatography, polyacrylamide-gel electrophoresis, and mass spectrometry.
- Analysis of large, complex macromolecules like DNA and proteins relies on techniques like x-ray crystallography and cryo-electron microscopy.
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