Cell Banking and Cell Line Establishment Quiz

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Which of the following is NOT a type of biological product manufactured through biomanufacturing?

Vaccines

Antibiotics (correct)

Hormones

Enzymes

What is the first step in Research and Development for manufacturing a therapeutic protein?

Identifying the gene of interest

Identifying the protein that interacts with the drug target

Identifying the drug target (correct)

Creating a cell line

What is the purpose of creating a cell line in the process of biomanufacturing?

To produce the therapeutic protein (correct)

To develop a vaccine

To interact with the drug target

To identify the gene of interest

Which of the following is NOT one of the sections in the Biomanufacturing class?

Downstream Bioprocessing Signup and view all the answers

What are biological products made from living cells called?

Biologic product Signup and view all the answers

Which type of cell is used to manufacture insulin?

Prokaryotic cell Signup and view all the answers

Which type of cell is used to manufacture Humira?

Eukaryotic cell Signup and view all the answers

What is the purpose of using an antibiotic-resistant gene in the plasmid?

To kill off the bacteria that do not contain the plasmid Signup and view all the answers

How are plasmids transferred into bacterial cells?

Through heat shock Signup and view all the answers

Why are liposomes used to transfer plasmids into mammalian cells?

Mammalian cells are more sensitive to environmental changes Signup and view all the answers

During upstream bioprocessing, what is the goal of growing production cells in increasingly larger bioreactors?

To maximize the number of cells producing the maximum amount of product Signup and view all the answers

What is the purpose of the production cells in the bioreactor?

To produce most products Signup and view all the answers

What happens when the bioreactor operates at maximum capacity?

The maximum number of cells are producing the maximum amount of product Signup and view all the answers

What is the purpose of upstream bioprocessing?

To grow production cells in increasingly larger bioreactors Signup and view all the answers

What is the result of the steps taken in upstream bioprocessing?

The maximum number of cells are producing the maximum amount of product Signup and view all the answers

Which type of cell is used to manufacture small, simple biologics?

Prokaryotic cell Signup and view all the answers

What is the reproduction time difference between bacterial cells and mammalian cells?

Bacterial cells divide every 20 to 30 minutes, while mammalian cells divide once a day on average. Signup and view all the answers

Why are mammalian cells more difficult to work with compared to bacterial cells?

Mammalian cells are more sensitive to changes in the cell culture environment. Signup and view all the answers

What type of cell line must be used to create therapeutic monoclonal antibodies?

Eukaryotic cell line Signup and view all the answers

Which mammalian cell line is currently the most common in biomanufacturing?

Chines Hamster Ovary (CHO) cell line Signup and view all the answers

Which of the following is NOT a goal of upstream bioprocessing?

Optimizing media composition Signup and view all the answers

What is a bioreactor used for in upstream bioprocessing?

Growing organisms under controlled conditions Signup and view all the answers

What is the purpose of scale-up in upstream bioprocessing?

To obtain optimum cell growth and protein production Signup and view all the answers

What is the role of bioreactors in upstream bioprocessing?

Growing cell cultures in large volumes Signup and view all the answers

Which of the following is the purpose of cell banking?

To store cells for future use in the production of biologic drugs Signup and view all the answers

Why is it important to go back to the original cell line?

Cells grow and change with time, so the original cell line must be accessed for consistency Signup and view all the answers

What is the difference between a master cell bank and a working cell bank?

Master cell banks are used for long-term storage, while working cell banks are used for starting manufacturing campaigns Signup and view all the answers

What is upstream bioprocessing?

The process of growing biological materials under controlled conditions to manufacture certain types of products Signup and view all the answers

Which of the following is NOT a step in Research and Development for manufacturing a therapeutic protein?

Creating a cell line that will produce the protein Signup and view all the answers

What is the purpose of a cell line in the process of biomanufacturing?

To produce the therapeutic protein Signup and view all the answers

Which of the following is NOT a type of biological product manufactured through biomanufacturing?

Antibiotics Signup and view all the answers

What are biological products made from living cells called?

Biologics Signup and view all the answers

What is the purpose of cell banking in biomanufacturing?

To store cell lines Signup and view all the answers

Which type of cells are used in upstream bioprocessing?

Mammalian cells Signup and view all the answers

What is the purpose of downstream bioprocessing?

To produce the maximum amount of product Signup and view all the answers

What is the goal of upstream bioprocessing?

To grow production cells in increasingly larger bioreactors Signup and view all the answers

What is the maximum capacity of the bioreactor?

The maximum number of cells Signup and view all the answers

What occurs during the first half of a campaign?

Upstream bioprocessing Signup and view all the answers

Which of the following is NOT a consideration when designing growth media for upstream bioprocessing?

concentration of carbohydrates Signup and view all the answers

What is the purpose of a bioreactor in upstream bioprocessing?

To optimize cell density and viability Signup and view all the answers

What is the purpose of scale-up in upstream bioprocessing?

To produce large volumes of protein-producing cells Signup and view all the answers

Which of the following is NOT a requirement for growth media in upstream bioprocessing?

Containing chemicals, viruses, and bacteria Signup and view all the answers

What is the purpose of optimizing cell density and viability in upstream bioprocessing?

To obtain consistent product Signup and view all the answers

Which of the following methods is used to transfer a plasmid into bacterial cells?

Heat shock and electroporation Signup and view all the answers

What is the purpose of the antibiotic-resistant gene in the plasmid?

To kill off bacteria that do not contain the plasmid Signup and view all the answers

Why are mammalian cells more difficult to work with compared to bacterial cells?

They are more sensitive to environmental changes Signup and view all the answers

What is the difference between prokaryotic and eukaryotic cells?

Prokaryotic cells do not have a nucleus, while eukaryotic cells do Signup and view all the answers

What is the purpose of cell lines in biomanufacturing?

To produce a particular product, such as therapeutic proteins Signup and view all the answers

Which of the following is a reason why cell banking is necessary in biomanufacturing?

To ensure the same therapeutic protein is made each time Signup and view all the answers

What is the purpose of creating a master cell bank in biomanufacturing?

To create a few hundred vials of cells Signup and view all the answers

What is the purpose of creating a working cell bank in biomanufacturing?

To create a few million cells Signup and view all the answers

Why is it important to access the original cell line in biomanufacturing?

To ensure the same therapeutic protein is made each time Signup and view all the answers

What is upstream bioprocessing in biomanufacturing?

The process of growing biological materials under controlled conditions Signup and view all the answers

Which type of cell is commonly used in biomanufacturing to produce monoclonal antibodies?

CHO cells Signup and view all the answers

What is the main difference between bacterial cells and mammalian cells in terms of growth and division?

Bacterial cells divide every 20 to 30 minutes, while mammalian cells divide once a day on average. Signup and view all the answers

Why are mammalian cells more sensitive to changes in the cell culture environment compared to bacterial cells?

Mammalian cells have a more complex structure than bacterial cells. Signup and view all the answers

Why are mammalian cells used to create large, complex proteins that require post-translational modifications?

Mammalian cells can perform post-therapeutic modifications. Signup and view all the answers

What is the purpose of adding an essential nutrient gene to the plasmid used in mammalian cell biomanufacturing?

To ensure only cells that produce the nutrient can survive. Signup and view all the answers

Cell Banking and Cell Line Establishment Quiz

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Questions and Answers

Which of the following is NOT a type of biological product manufactured through biomanufacturing?

What is the first step in Research and Development for manufacturing a therapeutic protein?

What is the purpose of creating a cell line in the process of biomanufacturing?

Which of the following is NOT one of the sections in the Biomanufacturing class?

What are biological products made from living cells called?

Which type of cell is used to manufacture insulin?

Which type of cell is used to manufacture Humira?

What is the purpose of using an antibiotic-resistant gene in the plasmid?

How are plasmids transferred into bacterial cells?

Why are liposomes used to transfer plasmids into mammalian cells?

During upstream bioprocessing, what is the goal of growing production cells in increasingly larger bioreactors?

What is the purpose of the production cells in the bioreactor?

What happens when the bioreactor operates at maximum capacity?

What is the purpose of upstream bioprocessing?

What is the result of the steps taken in upstream bioprocessing?

Which type of cell is used to manufacture small, simple biologics?

What is the reproduction time difference between bacterial cells and mammalian cells?

Why are mammalian cells more difficult to work with compared to bacterial cells?

What type of cell line must be used to create therapeutic monoclonal antibodies?

Which mammalian cell line is currently the most common in biomanufacturing?

Which of the following is NOT a goal of upstream bioprocessing?

What is a bioreactor used for in upstream bioprocessing?

What is the purpose of scale-up in upstream bioprocessing?

What is the role of bioreactors in upstream bioprocessing?

Which of the following is the purpose of cell banking?

Why is it important to go back to the original cell line?

What is the difference between a master cell bank and a working cell bank?

What is upstream bioprocessing?

Which of the following is NOT a step in Research and Development for manufacturing a therapeutic protein?

What is the purpose of a cell line in the process of biomanufacturing?

Which of the following is NOT a type of biological product manufactured through biomanufacturing?

What are biological products made from living cells called?

What is the purpose of cell banking in biomanufacturing?

Which type of cells are used in upstream bioprocessing?

What is the purpose of downstream bioprocessing?

What is the goal of upstream bioprocessing?

What is the maximum capacity of the bioreactor?

What occurs during the first half of a campaign?

Which of the following is NOT a consideration when designing growth media for upstream bioprocessing?

What is the purpose of a bioreactor in upstream bioprocessing?

What is the purpose of scale-up in upstream bioprocessing?

Which of the following is NOT a requirement for growth media in upstream bioprocessing?

What is the purpose of optimizing cell density and viability in upstream bioprocessing?

Which of the following methods is used to transfer a plasmid into bacterial cells?

What is the purpose of the antibiotic-resistant gene in the plasmid?

Why are mammalian cells more difficult to work with compared to bacterial cells?

What is the difference between prokaryotic and eukaryotic cells?

What is the purpose of cell lines in biomanufacturing?

Which of the following is a reason why cell banking is necessary in biomanufacturing?

What is the purpose of creating a master cell bank in biomanufacturing?

What is the purpose of creating a working cell bank in biomanufacturing?

Why is it important to access the original cell line in biomanufacturing?

What is upstream bioprocessing in biomanufacturing?

Which type of cell is commonly used in biomanufacturing to produce monoclonal antibodies?

What is the main difference between bacterial cells and mammalian cells in terms of growth and division?

Why are mammalian cells more sensitive to changes in the cell culture environment compared to bacterial cells?

Why are mammalian cells used to create large, complex proteins that require post-translational modifications?

What is the purpose of adding an essential nutrient gene to the plasmid used in mammalian cell biomanufacturing?

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