Cell and Tissue Staining Techniques

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

A researcher is examining a tissue sample and wants to specifically highlight the Golgi apparatus for detailed study. Which staining method would be most appropriate for this purpose?

  • Toluidine blue staining
  • Iron Hematoxylin staining
  • Sudan III staining
  • Silver staining (correct)

A pathologist observes a tissue sample where certain fibers stain red with Van Gieson's stain and others stain blue with Mallory trichrome. What type of fibers are being differentiated using these stains?

  • White and yellow collagenous fibers (correct)
  • Adipose and muscle fibers
  • Elastic and reticular fibers
  • Reticular and collagenous fibers

In a laboratory experiment, a student needs to identify glycogen deposits within liver tissue. Which of the following staining methods would best visualize glycogen?

  • Periodic acid Schiff (PAS) stain (correct)
  • Van Gieson's staining
  • Osmic acid staining
  • Sudan black staining

After performing a staining procedure, a technician observes that certain structures within a cell stain dark blue using Iron Hematoxylin (Iron H). Which cellular organelles are most likely highlighted by this staining?

<p>Mitochondria and centrioles (A)</p> Signup and view all the answers

A histologist is preparing a slide of adipose tissue and wants to clearly visualize the lipid droplets. Which staining technique would be most effective for this purpose?

<p>Sudan III staining (C)</p> Signup and view all the answers

A researcher is studying mast cells in a tissue sample. Which staining method would be most appropriate to specifically highlight these cells?

<p>Toluidine blue staining (C)</p> Signup and view all the answers

A researcher is examining a liver biopsy and needs to identify reticular fibers. Which staining method would best highlight these fibers?

<p>Silver staining (A)</p> Signup and view all the answers

While examining a stained tissue sample, a histologist observes cells with lipid droplets that appear 'signet ring'-like. Which staining technique most likely revealed these cells, and what cell type are they?

<p>H&amp;E staining, indicating unilocular fat cells (C)</p> Signup and view all the answers

A researcher is studying macrophages in a tissue culture and wants to visualize these cells dynamically. Which staining method would be most suitable for this purpose?

<p>Trypan blue (Vital) staining (A)</p> Signup and view all the answers

A pathologist is examining a sample of elastic tissue. Which of the following staining techniques would effectively highlight the elastic fibers?

<p>Ver-Hoff's staining (D)</p> Signup and view all the answers

Flashcards

Periodic acid–Schiff stain (PAS)

Stain used to identify glycogen, staining it magenta.

Sudan III

Stain for lipids, stains orange.

Ver-Hoff's stain

stain for elastic fibers, stains black.

Toluidine blue

Stain used to identify mast cells, staining them purple.

Signup and view all the flashcards

Van Gieson's stain

A method used to stain white collagenous fibers, staining red.

Signup and view all the flashcards

Golgi stain

Golgi stains a silver color, stains brown to black.

Signup and view all the flashcards

Iron stain

Iron accumulation stains dark blue.

Signup and view all the flashcards

Study Notes

  • Study notes on staining results for various cell/tissue types

Mitochondria

  • Stain dark blue with Iron Hematoxylin stain

Golgi Apparatus

  • Stains silver which turns brown to black

Centrioles

  • Stain dark blue with Iron Hematoxylin stain

Glycogen

  • Stains magenta with periodic acid–Schiff stain (PAS stain)
  • Stains red with Best's carmine stain

Lipid

  • Stains orange with Sudan III
  • Stains black with Sudan black
  • Stains black with osmic acid

White collagenous fibers

  • Stain red with Van Gieson's stain
  • Stains blue with Mallory trichrome stain

Yellow elastic fibers

  • Stain yellow with Van Gieson's stain
  • Stains black with Verhoeff's stain

Reticular Fibers

  • Stains silver, turning black

Unilocular fat cells

  • Signet ring with Hematoxylin and eosin stain (H&E stain)
  • Stains black with Sudan black
  • Stains black with osmic acid
  • Stains orange with Sudan III

Mast Cells

  • Stains purple with toluidine blue

Macrophages

  • Stains with trypan blue (Vital)

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Quiz Team

Related Documents

More Like This

Histology Staining Techniques Quiz
5 questions
Histology Staining Techniques
40 questions
Staining Techniques in Histology
24 questions
Histology Staining Techniques
5 questions
Use Quizgecko on...
Browser
Browser