Podcast
Questions and Answers
A researcher is examining a tissue sample and wants to specifically highlight the Golgi apparatus for detailed study. Which staining method would be most appropriate for this purpose?
A researcher is examining a tissue sample and wants to specifically highlight the Golgi apparatus for detailed study. Which staining method would be most appropriate for this purpose?
- Toluidine blue staining
- Iron Hematoxylin staining
- Sudan III staining
- Silver staining (correct)
A pathologist observes a tissue sample where certain fibers stain red with Van Gieson's stain and others stain blue with Mallory trichrome. What type of fibers are being differentiated using these stains?
A pathologist observes a tissue sample where certain fibers stain red with Van Gieson's stain and others stain blue with Mallory trichrome. What type of fibers are being differentiated using these stains?
- White and yellow collagenous fibers (correct)
- Adipose and muscle fibers
- Elastic and reticular fibers
- Reticular and collagenous fibers
In a laboratory experiment, a student needs to identify glycogen deposits within liver tissue. Which of the following staining methods would best visualize glycogen?
In a laboratory experiment, a student needs to identify glycogen deposits within liver tissue. Which of the following staining methods would best visualize glycogen?
- Periodic acid Schiff (PAS) stain (correct)
- Van Gieson's staining
- Osmic acid staining
- Sudan black staining
After performing a staining procedure, a technician observes that certain structures within a cell stain dark blue using Iron Hematoxylin (Iron H). Which cellular organelles are most likely highlighted by this staining?
After performing a staining procedure, a technician observes that certain structures within a cell stain dark blue using Iron Hematoxylin (Iron H). Which cellular organelles are most likely highlighted by this staining?
A histologist is preparing a slide of adipose tissue and wants to clearly visualize the lipid droplets. Which staining technique would be most effective for this purpose?
A histologist is preparing a slide of adipose tissue and wants to clearly visualize the lipid droplets. Which staining technique would be most effective for this purpose?
A researcher is studying mast cells in a tissue sample. Which staining method would be most appropriate to specifically highlight these cells?
A researcher is studying mast cells in a tissue sample. Which staining method would be most appropriate to specifically highlight these cells?
A researcher is examining a liver biopsy and needs to identify reticular fibers. Which staining method would best highlight these fibers?
A researcher is examining a liver biopsy and needs to identify reticular fibers. Which staining method would best highlight these fibers?
While examining a stained tissue sample, a histologist observes cells with lipid droplets that appear 'signet ring'-like. Which staining technique most likely revealed these cells, and what cell type are they?
While examining a stained tissue sample, a histologist observes cells with lipid droplets that appear 'signet ring'-like. Which staining technique most likely revealed these cells, and what cell type are they?
A researcher is studying macrophages in a tissue culture and wants to visualize these cells dynamically. Which staining method would be most suitable for this purpose?
A researcher is studying macrophages in a tissue culture and wants to visualize these cells dynamically. Which staining method would be most suitable for this purpose?
A pathologist is examining a sample of elastic tissue. Which of the following staining techniques would effectively highlight the elastic fibers?
A pathologist is examining a sample of elastic tissue. Which of the following staining techniques would effectively highlight the elastic fibers?
Flashcards
Periodic acid–Schiff stain (PAS)
Periodic acid–Schiff stain (PAS)
Stain used to identify glycogen, staining it magenta.
Sudan III
Sudan III
Stain for lipids, stains orange.
Ver-Hoff's stain
Ver-Hoff's stain
stain for elastic fibers, stains black.
Toluidine blue
Toluidine blue
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Van Gieson's stain
Van Gieson's stain
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Golgi stain
Golgi stain
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Iron stain
Iron stain
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Study Notes
- Study notes on staining results for various cell/tissue types
Mitochondria
- Stain dark blue with Iron Hematoxylin stain
Golgi Apparatus
- Stains silver which turns brown to black
Centrioles
- Stain dark blue with Iron Hematoxylin stain
Glycogen
- Stains magenta with periodic acid–Schiff stain (PAS stain)
- Stains red with Best's carmine stain
Lipid
- Stains orange with Sudan III
- Stains black with Sudan black
- Stains black with osmic acid
White collagenous fibers
- Stain red with Van Gieson's stain
- Stains blue with Mallory trichrome stain
Yellow elastic fibers
- Stain yellow with Van Gieson's stain
- Stains black with Verhoeff's stain
Reticular Fibers
- Stains silver, turning black
Unilocular fat cells
- Signet ring with Hematoxylin and eosin stain (H&E stain)
- Stains black with Sudan black
- Stains black with osmic acid
- Stains orange with Sudan III
Mast Cells
- Stains purple with toluidine blue
Macrophages
- Stains with trypan blue (Vital)
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