Histology Tissue Quiz: Cell and Tissue Structure

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the main reason histology depends on microscopes and molecular methods?

The smooth texture of cells and matrix components

Cells and matrix components are too large to be seen with the naked eye

The bright colors of cells and matrix components

The small size of cells and matrix components (correct)

Why are thin translucent sections prepared from tissues for microscopic examination?

To make tissues opaque

To make tissues larger

To allow light to pass through tissues (correct)

To enhance the thickness of tissues

What is the main purpose of preparing tissue slices or sections in histologic research?

To increase the thickness of tissues

To remove all internal structures from tissues

To visualize internal structures of tissues (correct)

To examine tissues with electron microscopes

Which branch of science is essential for a proper understanding of tissue biology?

Biochemistry Signup and view all the answers

Why are advances in biochemistry, molecular biology, physiology, immunology, and pathology considered essential for a better knowledge of tissue biology?

To provide insights into tissue functions Signup and view all the answers

Which method is commonly used to study cells and tissues according to the text?

Preparation of tissue slices for microscopic examination Signup and view all the answers

What is the primary focus of the study of histology?

The arrangement and interactions of different tissues within organs Signup and view all the answers

What are the two main interacting components of tissues?

Cells and the extracellular matrix Signup and view all the answers

What is the primary function of the extracellular matrix (ECM)?

All of the above Signup and view all the answers

How are cells and the extracellular matrix connected?

All of the above Signup and view all the answers

How do cells and their associated matrix become specialized during development?

They become functionally specialized and give rise to fundamental types of tissues with characteristic structural features. Signup and view all the answers

Which of the following is NOT a key component of tissues?

Mitochondria Signup and view all the answers

What is the primary function of chemical fixatives in the preparation of tissues for histological study?

To preserve the natural structure and composition of the tissue Signup and view all the answers

What is the purpose of dehydrating and clearing the fixed tissue before embedding?

To remove water and replace it with a medium that can be easily sectioned Signup and view all the answers

What is the primary purpose of staining tissue sections mounted on glass slides?

To reveal specific cellular and tissue components under the microscope Signup and view all the answers

What is the significance of the terms "basophilic" and "acidophilic" in the context of tissue staining?

They refer to the ability of a tissue component to absorb basic or acidic stains Signup and view all the answers

What is the primary advantage of using fluorescence microscopy compared to bright-field microscopy?

Fluorescence microscopy can visualize specific molecules or structures labeled with fluorescent probes Signup and view all the answers

Which of the following is the most commonly used staining method in histology?

Hematoxylin and eosin (H&E) staining Signup and view all the answers

Study Notes

Histology and Its Methods of Study

Histology is the study of the tissues of the body and how these tissues are arranged to constitute organs.
Tissues have two interacting components: cells and extracellular matrix (ECM).
The ECM consists of many kinds of macromolecules, such as collagen fibrils, which support the cells and contain fluids transporting nutrients to the cells and carrying away their wastes and secretory products.

Preparation of Tissues for Study

Chemical fixatives like formalin are used to preserve tissue structure by cross-linking and denaturing proteins, inactivating enzymes, and preventing cell autolysis or self-digestion.
Dehydration of the fixed tissue in alcohol and clearing in organic solvents prepare it for embedding and sectioning.
Embedding in paraffin wax or epoxy resin allows the tissue to be cut into very thin sections (slices) with a microtome.
Sections are mounted on glass slides for staining, which is required to reveal specific cellular and tissue components with the microscope.

Staining and Microscopy

The most commonly used staining method is a combination of the stains H&E, which act as basic and acidic dyes, respectively.
Cell substances with a net negative (anionic) charge, such as DNA and RNA, react strongly with hematoxylin and basic stains and are said to be “basophilic.”
Cationic substances, such as collagen and many cytoplasmic proteins react with eosin and other acidic stains and are said to be “acidophilic.”
Bright-field microscopy uses ordinary light and the colors are imparted by tissue staining.
Fluorescence microscopy uses UV light, under which only fluorescent molecules are visible, allowing localization of fluorescent probes which can be much more specific than routine stains.

Other Microscopy Techniques

Phase-Contrast Microscopy
Confocal Microscopy
Polarizing Microscopy
Autoradiography
Transmission Electron Microscopy
Scanning Electron Microscopy

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Description

Test your knowledge on the formation and arrangement of tissues to create organs, as well as the study methods and advancements in histology. Explore topics like cell and tissue culture, enzyme histochemistry, and visualizing specific molecules.

Cell and Tissue Histology Quiz

Choose a study mode