Cartilage and Bone Quiz
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Questions and Answers

What is the primary characteristic of the matrix in cartilage?

  • It is rigid and brittle.
  • It is entirely solid and non-flexible.
  • It is rubbery and flexible. (correct)
  • It is fluid-like and unstable.
  • Which of the following cell types are found in cartilage?

  • Adipocytes and macrophages.
  • Osteoblasts and osteocytes.
  • Fibroblasts and mast cells.
  • Chondroblasts and chondrocytes. (correct)
  • What is the role of the perichondrium in cartilage?

  • It is responsible for the formation of hyaline cartilage.
  • It acts as a rigid outer layer.
  • It provides nerve supply to the cartilage.
  • It serves as a nutrient source via diffusion. (correct)
  • What are the three types of cartilage based on?

    <p>Amount of ground substance and type of fibers. (D)</p> Signup and view all the answers

    How does cartilage provide support to soft tissues?

    <p>By offering some flexibility. (D)</p> Signup and view all the answers

    What is the primary function of chondrocytes in cartilage?

    <p>To form small nests within lacunae (C)</p> Signup and view all the answers

    What type of cartilage is found in the intervertebral disc?

    <p>Fibrocartilage (C)</p> Signup and view all the answers

    Which structure primarily provides strength and limited mobility at joints?

    <p>Annulus fibrosus (B)</p> Signup and view all the answers

    What occurs when there is herniation of the nucleus pulposus?

    <p>Compression of nerve roots (A)</p> Signup and view all the answers

    Which of the following is a characteristic of cartilage?

    <p>It is flexible and can recover its shape after deformation (B)</p> Signup and view all the answers

    What occurs at the primary ossification center?

    <p>Increased chondrogenic activity leads to the formation of periosteum. (B), Blood vessels invade irregular cavities to create compact bone. (D)</p> Signup and view all the answers

    In which part of a long bone does the secondary ossification center develop?

    <p>Epiphysis (C)</p> Signup and view all the answers

    Which zone of the epiphyseal cartilage involves the accumulation of glycogen and alkaline phosphatase enzyme?

    <p>Zone of hypertrophy (C)</p> Signup and view all the answers

    What is the function of osteoblasts during the ossification process?

    <p>To form a layer of periosteal bone collar. (D)</p> Signup and view all the answers

    What occurs in the zone of invasion of the epiphyseal cartilage?

    <p>Blood vessels and U.M.C form a vascular bud. (A)</p> Signup and view all the answers

    What is the appearance of chondrocytes during tissue preparation?

    <p>They shrink, making lacunae appear empty. (C)</p> Signup and view all the answers

    What is formed by clusters of dividing chondrocytes?

    <p>Isogenous groups (D)</p> Signup and view all the answers

    What is the primary function of protein-forming cells in cartilage?

    <p>To maintain the matrix through continuous secretion. (A)</p> Signup and view all the answers

    What type of growth does cartilage undergo when young chondrocytes divide?

    <p>Interstitial growth (C)</p> Signup and view all the answers

    What is a characteristic of elastic cartilage?

    <p>Contains a large number of branching elastic fibers. (A)</p> Signup and view all the answers

    What changes occur in old chondrocytes?

    <p>They accumulate large lipid droplets and glycogen. (A)</p> Signup and view all the answers

    What type of collagen is primarily formed in cartilage?

    <p>Collagen type II (B)</p> Signup and view all the answers

    What distinguishes tough cartilage from hyaline cartilage?

    <p>It is opaque and not flexible. (C)</p> Signup and view all the answers

    What initiates the acute inflammatory response during the healing process of a fracture?

    <p>Cytokines from platelet degranulation (C)</p> Signup and view all the answers

    Which method allows for the demonstration of bone cells and soft tissue?

    <p>Decalcification method (A)</p> Signup and view all the answers

    What is characteristic of the grinding method used on bones?

    <p>Ground sections show bone lamellae and lacunae (C)</p> Signup and view all the answers

    Which type of bone is classified as compact bone?

    <p>Long bone (B)</p> Signup and view all the answers

    What is found under the periosteum of a long bone?

    <p>External circumferential lamellae (C)</p> Signup and view all the answers

    What are Haversian systems also known as?

    <p>Osteons (C)</p> Signup and view all the answers

    Which of the following statement regarding Volkmann's canal is correct?

    <p>It runs perpendicular to Haversian canals (A)</p> Signup and view all the answers

    Which anatomical classification refers to bones shaped like cubes?

    <p>Short bones (A)</p> Signup and view all the answers

    What is the tubular system formed with T-tubules called in cardiac muscle at the level of Z-line?

    <p>Diad (B)</p> Signup and view all the answers

    Where are glycogen granules mainly concentrated in cardiac muscle cells?

    <p>Between myofibrils (D)</p> Signup and view all the answers

    Which hormone is contained in atrial granules within the heart?

    <p>Atrial natriuretic hormone (C)</p> Signup and view all the answers

    What do desmosomes and adherent junctions do in cardiac muscle cells?

    <p>Prevent separation during contractions (C)</p> Signup and view all the answers

    What shape do the discs of cardiac muscle cells resemble?

    <p>Stairway (B)</p> Signup and view all the answers

    What is located in the right ventricle and contains Purkinje muscle fibers?

    <p>Moderator band (C)</p> Signup and view all the answers

    What is a characteristic feature of Purkinje muscle fibers compared to ordinary cardiac muscle fibers?

    <p>Larger diameter (C)</p> Signup and view all the answers

    What prevents gap junctions in cardiac muscle cells from being damaged during contractions?

    <p>Lateral position within the disc (D)</p> Signup and view all the answers

    Flashcards

    What is cartilage?

    Cartilage is a specialized type of connective tissue with a rubbery matrix, allowing it to withstand mechanical stress.

    How does cartilage receive nutrients?

    Cartilage is avascular, meaning it lacks blood vessels. It receives oxygen and nutrients from the surrounding connective tissue (perichondrium) or synovial fluid in joints.

    What cells make up cartilage?

    Cartilage is made up of chondroblasts and chondrocytes, cells responsible for producing the matrix.

    What is hyaline cartilage?

    Hyaline cartilage is the most common type, translucent, and has a glassy appearance. It's found in various parts of the body, including the fetal skeleton, articular surfaces of bones, costal cartilage, epiphyseal plate, and upper respiratory passages.

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    What is the perichondrium?

    The perichondrium is a capsule-like layer surrounding hyaline cartilage, except at the articular surface of joints. It consists of an outer fibrous layer and an inner cellular layer.

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    Chondrocytes

    Cells found individually within small spaces called lacunae in cartilage tissue.

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    Chondroblasts

    Young cartilage cells that actively produce and secrete the extracellular matrix of cartilage.

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    Interstitial Growth

    The process of cartilage growth from the inside, where existing chondrocytes divide and produce new matrix around themselves.

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    Appositional Growth

    The process of cartilage growth from the outside, where new chondroblasts are added to the perichondrium and produce matrix.

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    Elastic Cartilage

    A type of cartilage that is tough and resilient, containing a dense network of collagen fibers.

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    Hyaline Cartilage

    A type of cartilage that is smooth and flexible, with a glassy appearance, containing a fine network of collagen fibers.

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    Fibrocartilage

    A type of cartilage that is strong and dense, containing thick bundles of collagen fibers, found in areas of high stress.

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    Perichondrium

    The thin layer of connective tissue that surrounds most cartilage, providing nutrients and support.

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    What are chondrocytes?

    Cartilage cells that reside in small cavities called lacunae. These cells often form clusters of 2 or more, known as isogenous groups, due to their division.

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    What is elastic cartilage?

    A type of cartilage with high flexibility and resilience. It returns to its original shape after being deformed. Found in ear pinna, nose, trachea, and some laryngeal cartilages.

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    What is the annulus fibrosus?

    The outer ring of an intervertebral disc composed of white fibrocartilage, rich in collagen type I fibers.

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    What is the nucleus pulposus?

    The inner, jelly-like core of an intervertebral disc composed of collagen type II. If it protrudes through the annulus fibrosus, it can cause nerve compression, leading to pain.

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    Endochondral ossification

    The process where cartilage is replaced by bone, occurring in long, short and irregular bones.

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    Primary Ossification Center

    A primary ossification center appears in the middle of the diaphysis of the cartilage model, where chondrogenic cells transform into osteogenic cells, eventually forming osteoblasts.

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    Secondary Ossification Center

    A secondary ossification center forms in the epiphysis of the bone, following similar steps to the primary center, ultimately leading to the replacement of cartilage with spongy bone.

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    Periosteal bone collar

    The perichondrium, the connective tissue surrounding cartilage, transforms into the periosteum, which then forms a layer of bone called the periosteal bone collar.

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    Zone of Calcification

    This zone in the epiphyseal cartilage contains chondrocytes that deposit calcium in the surrounding matrix, ultimately leading to the calcification of the cartilage.

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    Intercalated Discs

    Specialized junctions between adjacent cardiac muscle cells, enabling coordinated contractions.

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    Gap Junctions (in Intercalated Discs)

    Gap junctions located in the lateral segment of intercalated discs, allowing electrical signals to pass rapidly between cardiac muscle cells.

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    Transverse Component of Intercalated Discs

    A transverse component of the intercalated discs that crosses cardiac myofibers at a right angle, like the risers of a staircase.

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    Lateral Component of Intercalated Discs

    A lateral component of the intercalated discs that lies parallel to myofibers, like the steps of a staircase.

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    Desmosomes & Adherent Junctions

    Desmosomes and adherent junctions found in the transverse region of intercalated discs, firmly binding cardiac myocytes together.

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    Purkinje Fibers

    Specialized cardiac muscle fibers in the atrioventricular (AV) bundle, quickly transmitting contractile impulses to ventricular cells.

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    Moderator Band

    A band of Purkinje fibers in the right ventricle.

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    Purkinje Fibers' Sarcoplasm

    Cardiac muscle cells with a pale, vacuolated sarcoplasm due to high glycogen content.

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    Decalcification: How does it work?

    A decalcification method used in bone histology where calcium is removed from bone samples using mineral acids (e.g., nitric acid 10%). This softens the bone, making it suitable for sectioning and staining.

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    Grinding method: What does it show?

    A histological technique where bone samples are dried and then progressively thinned using a carborundum wheel. This method reveals the bone matrix structure, including lamellae, lacunae, and canaliculi, but does not show bone cells.

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    Periosteum: What is it?

    The outer layer covering the surface of a bone, composed of dense connective tissue. It plays a crucial role in bone growth, repair, and blood vessel attachment.

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    External circumferential lamellae: What are they?

    Concentrically arranged layers of calcified collagen fibers that form the basic structural unit of compact bone. Each osteon has a central canal (containing blood vessels and nerves) surrounded by these lamellae.

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    Haversian system (osteon): What is it?

    The functional unit of compact bone, a cylindrical structure consisting of a central canal (Haversian canal), containing blood vessels and nerves, surrounded by concentric lamellae. Osteocytes are located within lacunae in the lamellae.

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    Volkmann's canal: What is it?

    Transverse or oblique canals that connect Haversian canals, allowing for communication between different osteons. They also serve as pathways for blood vessels and nerves.

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    Lacunae: What do they contain?

    Small cavities within the bone matrix that house osteocytes. These cavities are interconnected by tiny channels called canaliculi, allowing for nutrient exchange and communication between osteocytes.

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    Canaliculi: What are they?

    Tiny channels that connect lacunae, allowing for communication and nutrient exchange between osteocytes. They form a network within the bone matrix.

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    Study Notes

    Cartilage

    • Specialized connective tissue with a rubbery matrix, designed to withstand mechanical stress.
    • Composed of chondroblasts (cells that produce matrix) and chondrocytes (cells within matrix).
    • Matrix includes ground substance (abundant, firm, and rubbery), and collagen and elastic fibers.
    • Avascular; relies on diffusion of oxygen and nutrients from surrounding connective tissues (perichondrium) or synovial fluid in joints.
    • Lacks blood vessels and nerves.
    • Three types: hyaline, elastic, and fibrocartilage, distinguished by the type and amount of fibers in the matrix.

    Hyaline Cartilage

    • Most common type, appearing translucent and glassy.
    • Found in fetal skeletons, articular surfaces of bones, costal cartilage, and respiratory passages (nose, larynx, trachea, and bronchi).
    • Matrix is mostly collagen type II.
    • Chondrocytes are arranged in lacunae and are typically in small groups.
    • Surrounded by perichondrium (a layer of dense connective tissue).
    • Supports soft tissues with flexibility, helps with weight-bearing and shock absorption, and facilitates easy movement at joints.

    Perichondrium

    • Capsule-like covering around hyaline cartilage (except at articular surfaces).

    • Consists of two layers: outer fibrous (white fibrous connective tissue) containing fibroblasts that secrete collagen type I, as well as blood vessels and nerves; inner chondrogenic (cellular) with chondroblasts, crucial for cartilage nutrition (by diffusion).

    • Essential for cartilage nutrition and growth, and also for attachment of muscles.

    • Collagen fibers: Type II, not visible by light microscopy (LM) due to their thinness and similar refractive index to the ground substance. Visible after digestion of the matrix by enzymes.

    • Ground substance: produced by chondrocytes and chondroblasts, rubbery, homogeneous, and transparent; characterized by its high content of proteoglycans, glycoproteins, and water; stained metachromatically (e.g., with toluidine blue).

    Cartilage Cells

    • Chondroblasts: young cells that actively produce cartilage matrix, flattened or oval with a basophilic cytoplasm and pale nucleus; located on the surface of cartilage; give rise to chondrocytes.
    • Chondrocytes: mature cells maintained within spaces (lacunae) located within the matrix, rounded cells; typically seen within groups, with pale cytoplasm and a darker-staining nucleus; become trapped as the matrix hardens around the cells.

    Cartilage Function

    • Supports soft tissues with flexibility
    • Keeps airway patent (open)
    • Tissue attachment & weight bearing, shock absorbing
    • Smooth surface for easy movement of bones
    • Development and growth of bones

    Bone

    • Highly vascularized, hard connective tissue composed of a calcified matrix.
    • Provides support, protection (e.g., brain, bone marrow), and mineral storage (mainly calcium).
    • Matrix: organic (collagen fibers, ground substance) and inorganic (calcium salts).
    • Covers bone surface: periosteum (outer) and endosteum (inner).
    • Three types of cells (in order): osteogenic, osteoblasts, and osteocytes, and osteoclasts.

    Bone Composition

    • Organic Parts (35%): Type I collagen fibers in thick bundles and ground substance (glycosaminoglycans, glycoproteins).
    • Inorganic Parts (65%): Calcium salts (mainly calcium phosphate and carbonate) deposited within and on surface of collagen bundles. These give the bone its hardness.

    Bone Coverings

    • Periosteum: Tough, fibrous outer layer of dense irregular connective tissue; contains blood vessels and nerves; covers outer surface of bone, except at articular surfaces. Essential for bone nutrition, growth, and repair, and attachment of tendons and ligaments.
    • Endosteum: Delicate inner layer of connective tissue; lining the bone's inner surface, including the medullary cavity and trabeculae; contains osteogenic cells that aid in bone growth by adding from inside (internal growth), in bone repair, and nutrition of the bone surface

    Bone Covering Function

    • Periosteum:
    • Protection of bone
    • Attachment for muscles, tendons, ligaments.
    • Bone nutrition (through blood vessels)
    • Growth and repair
    • Endosteum:
    • Protection of bone surface
    • Bone growth from the inside (internal growth)
    • Essential part of bone remodeling

    Bone Cells

    • Osteogenic cells: Undifferentiated mesenchymal stem cells from the periosteum or endosteum. Give rise to osteoblasts.
    • Osteoblasts: Immature bone-forming cells; found on bone surfaces. Secrete a matrix composed mainly of collagen and proteins, which later calcifies to make bone.
    • Osteocytes: Mature osteoblasts that are encased in the bone matrix; maintain bone tissue. Connect to each other through canaliculi - allowing them to communicate via processes in the hard matrix.
    • Osteoclasts: Bone-resorbing cells; participate in remodeling and regulate calcium homeostasis. Large, multinucleated cells that breakdown bone tissue by decalcification.

    Bone Classifications

    • Anatomical: Long, short, flat, irregular

    • Histological: Compact, spongy

    • Compact Bone: Dense, outer layer of bone; composed of osteons (Haversian systems), cylindrical units of calcified bone lamellae surrounding a central canal with nerves and blood vessels. Interstitial lamellae fill gaps between osteons. Outer circumferential and inner circumferential lamellae surround the entire outer/inner surface of compact bone respectively.

    • Spongy Bone: Porous inner layer of bone, found in the interior of flat bones, and within epiphyses of long bones; consists of trabeculae, interlocking bony struts, filled with bone marrow; Provides strength and lightness.

    • Volkmann's canals: Canals that traverse compact bone and connect Haversian canals with bone marrow and periosteum.

    • Perforating fibers of Sharpey: Collagen fibers that attach tendons and ligaments to the bone matrix.


    Muscle Tissue

    • Muscle tissue specialized for contraction, facilitating movement.
    • Divided into three types - skeletal, cardiac, and smooth - each possessing distinct structural and functional characteristics.

    Skeletal Muscle Tissue

    • Attaches to bones, enabling voluntary movement.
    • Composed of elongated muscle cells called fibers.
    • Striated appearance due to orderly arrangement of contractile proteins.
    • Multinucleated, with nuclei located peripherally.
    • Requires signal from the nervous system to contract.

    Cardiac Muscle Tissue

    • Found exclusively in the heart.
    • Branched, striated appearance.
    • Intercalated discs (specialized junctions) connect adjacent cells, facilitating rapid transmission of signals for coordinated contraction.
    • Involuntary movement.
    • Single nucleus located centrally.

    Smooth Muscle Tissue

    • Located in walls of internal organs and blood vessels.
    • Non-striated appearance, with a spindle-shaped cell form.
    • Single nucleus in the cell's center.
    • Involuntary contraction, controlled by autonomic nervous system and hormones.

    Skin

    • Protective covering of the body.
    • Two main layers - epidermis, dermis and hypodermis
    • Hypodermis - important for insulation.
    • The function of the skin is protection.
    • Skin repairs through various cells.
    • Also a site for many receptors and cell communication

    Epidermis

    • Outermost layer of skin, mostly keratinized stratified squamous epithelium.
    • Avascular, nutrition by diffusion from dermis.
    • Consists of five layers — Stratum basale, Stratum spinosum, Stratum granulosum, Stratum lucidum, and Stratum corneum.

    Dermis

    • Deep layer of skin, consisting of connective tissue (CT), rich in collagen and elastin fibers.
    • Responsible for skin's strength, flexibility, and elasticity.
    • Contains blood vessels, nerves, and specialized sensory receptors.
    • Two sublayers - papillary layer and reticular layer.

    Hypodermis

    • Subcutaneous tissue, not a part of skin.
    • Layer below dermis, highly vascular, mostly adipose CT, providing insulation and cushioning.
    • Connects skin to underlying tissues.

    Skin Appendages

    • (Hair Follicles):
    • Epithelial tube extending into the dermis or hypodermis.
    • Contains hair shaft (outer, part of the hair shaft).
    • Muscle bundles which attached to hair follicle.
    • (Sebaceous glands):
    • Simple alveolar glands associated with hair follicles; secrete sebum (oily substance) that keeps hair and skin soft and waterproof.
    • (Sweat glands):
    • Produce sweat (water, salts, urea); responsible for thermoregulation and excretion. Two types — eccrine (most abundant) and apocrine (associated with hair follicles; larger secretion volume).

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