Carbohydrate Fermentation Tests

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What is the primary function of carbohydrate fermentation tests?

To identify the type of bacteria

To determine the presence of enzymes in bacteria

To provide energy to bacteria (correct)

To break down gelatin into glucose

What is the indicator used in the carbohydrate fermentation tests?

Amylase

Gelatin

Gram's Iodine

Phenol Red (correct)

What is the result of a positive gelatinase test?

No liquefaction of gelatin

Production of acid

Production of gas

Liquefaction of gelatin (correct)

What is the purpose of adding Gram's Iodine solution to the starch agar plate in the amylase test?

To detect the presence of starch Signup and view all the answers

What is the result of a negative amylase test?

No clear halo around the colony Signup and view all the answers

What is the medium used in the carbohydrate fermentation tests?

Glucose PRB Signup and view all the answers

What is the purpose of the peptone in the carbohydrate fermentation tests?

To change the color of the medium Signup and view all the answers

What is the significance of a pH decrease in the carbohydrate fermentation tests?

The bacteria have produced acid Signup and view all the answers

What is the result of a positive carbohydrate fermentation test?

Acid or gas is produced Signup and view all the answers

What is the purpose of using the '2/3 of needle' technique in the exoenzyme tests?

To inoculate the medium with bacteria Signup and view all the answers

What is the primary function of the enzyme urease in the Urease Test?

To produce ammonia Signup and view all the answers

What is the purpose of adding 3% H2O2 in the Oxidase Test?

To detect the production of cytochrome C oxidase Signup and view all the answers

In the Catalase Test, what is the purpose of rubbing the applicator tip on a well-isolated colony?

To release the enzyme catalase from the bacteria Signup and view all the answers

What is the significance of a positive Indole Test result?

The bacteria can produce tryptophanase Signup and view all the answers

What is the primary function of the enzyme citritase in the Citrate Test?

To utilize citrate as a carbon source Signup and view all the answers

What is the purpose of the Methyl Red Test?

To detect mixed acid fermentation Signup and view all the answers

What is the significance of a positive Hydrogen Sulfide Test result?

The bacteria can produce hydrogen sulfide gas Signup and view all the answers

What is the purpose of the TSA Plate in the Catalase Test?

To provide a medium for the growth of bacteria Signup and view all the answers

What is the purpose of the IMViC tests?

To determine the genus of bacteria Signup and view all the answers

What is the significance of a negative Oxidase Test result?

The bacteria cannot produce cytochrome C oxidase Signup and view all the answers