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Questions and Answers
What are the two components that constitute the process of fluidization in packed bed bioreactors?
What are the two components that constitute the process of fluidization in packed bed bioreactors?
The two components are the solid particles that are fluidized and the fluidizing medium, which is either a liquid or gas.
Describe the role of the nutrient broth in a packed bed bioreactor.
Describe the role of the nutrient broth in a packed bed bioreactor.
The nutrient broth flows continuously over the immobilized biocatalyst, providing the necessary nutrients for biochemical reactions.
What is the purpose of downstream processing in bioprocessing?
What is the purpose of downstream processing in bioprocessing?
Downstream processing is aimed at isolating and purifying the biotechnological product to meet required purity and quality standards.
List three stages involved in downstream processing and briefly describe one.
List three stages involved in downstream processing and briefly describe one.
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What is one method used for cell disruption in downstream processing?
What is one method used for cell disruption in downstream processing?
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What are the environmental benefits of solid state fermentation?
What are the environmental benefits of solid state fermentation?
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How does solid state fermentation utilize agro-industrial residues?
How does solid state fermentation utilize agro-industrial residues?
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What advantages does solid state fermentation offer in terms of technology and investment?
What advantages does solid state fermentation offer in terms of technology and investment?
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Describe the fluidization process in fluidized bed bioreactors.
Describe the fluidization process in fluidized bed bioreactors.
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What is the purpose of expanding the top position in the design of fluidized bed bioreactors?
What is the purpose of expanding the top position in the design of fluidized bed bioreactors?
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Study Notes
Solid State Fermentation (SSF) Advantages
- Produces minimal waste and liquid effluents, reducing environmental impact.
- Utilizes simple natural solids as media, promoting sustainability.
- Requires low technology and energy, resulting in lower capital investment.
- No sterilization needed, reducing microbial contamination risks.
- Simplified downstream processing enhances efficiency.
- Leverages agro-industrial residues as substrates, adding value to underutilized materials.
- High product yield is achievable with SSF methodologies.
- Bioreactor designs and aeration methods are straightforward and manageable.
- Diverse domestic, industrial, and agricultural wastes can be utilized effectively in SSF.
Fluidized Bed Bioreactors
- Structure allows for the expansion of the top to decrease fluid velocity.
- Solids are retained while liquid is permitted to flow out, maintaining separation.
- Fluidization happens when small solid particles are suspended in an upward fluid stream.
- Fluid velocity is calibrated to suspend particles without expelling them from the reactor.
- High mixing efficiency is achieved as solid particles rapidly swirl in the fluidized state.
- The solid typically fluidized is either a liquid or gas medium.
Packed Bed Bioreactors
- Comprises a column packed with solid particles containing biocatalysts.
- Solids may be porous or non-porous gels, facilitating the reaction.
- Used solids can be compressible or rigid, influencing bioreactor performance.
- Nutrient broth circulates continuously over immobilized biocatalysts to support growth and activity.
Downstream Processing (DSP)
- Refers to the stage of bioprocessing where cell mass is processed for purity and quality.
- DSP involves isolating and purifying biotechnological products for intended applications.
- Different stages of downstream processing include:
- Harvest: Solid-liquid separation through centrifugation or filtration.
- Concentration: Techniques such as ultrafiltration, diafiltration, and precipitation.
- Purification: Achieved via chromatography to enhance product purity.
- Formulation: Includes crystallization and freeze drying for product stability.
- Filling: Packaged products are prepared for market distribution.
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Description
Explore the principles of Solid State Fermentation (SSF) in Industrial Microbiology. This quiz covers the advantages of SSF, including environmental benefits and cost-effectiveness. Test your knowledge on how SSF minimizes waste and microbial contamination.
