Podcast
Questions and Answers
Which of the following statements about the Nernst equation is TRUE?
Which of the following statements about the Nernst equation is TRUE?
Which of the following is NOT a characteristic of active transport?
Which of the following is NOT a characteristic of active transport?
Which of the following statements accurately describes the role of F1F0-ATP synthase in oxidative phosphorylation?
Which of the following statements accurately describes the role of F1F0-ATP synthase in oxidative phosphorylation?
What is the primary driving force behind protein folding?
What is the primary driving force behind protein folding?
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Which of the following processes is NOT directly coupled to the electron transport chain in mitochondria?
Which of the following processes is NOT directly coupled to the electron transport chain in mitochondria?
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What is primarily studied within the context of light energy transduction in chloroplasts?
What is primarily studied within the context of light energy transduction in chloroplasts?
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Which of the following is a recommended reading that focuses on the principles of bioenergetics?
Which of the following is a recommended reading that focuses on the principles of bioenergetics?
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Which edition of 'Molecular Biology of the Cell' is listed as a recommended reading resource?
Which edition of 'Molecular Biology of the Cell' is listed as a recommended reading resource?
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What is the purpose of photophosphorylation within chloroplasts?
What is the purpose of photophosphorylation within chloroplasts?
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Which source would provide lecture notes on thermodynamics?
Which source would provide lecture notes on thermodynamics?
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Within metabolic studies, which chapter from 'Brock Biology of Microorganisms' is highlighted?
Within metabolic studies, which chapter from 'Brock Biology of Microorganisms' is highlighted?
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What aspect of membranes is described in the current lecture mentioned?
What aspect of membranes is described in the current lecture mentioned?
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Which of the following is NOT an aspect of bioenergetics discussed in the recommended resources?
Which of the following is NOT an aspect of bioenergetics discussed in the recommended resources?
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What role does the externalization of phosphatidylserine play in cells?
What role does the externalization of phosphatidylserine play in cells?
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Which mechanism contributes to the maintenance of lipid asymmetry in the plasma membrane?
Which mechanism contributes to the maintenance of lipid asymmetry in the plasma membrane?
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What causes the randomization of phosphatidylserine in apoptotic cells?
What causes the randomization of phosphatidylserine in apoptotic cells?
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What is a consequence of the disruption of ATP supply in relation to membrane asymmetry?
What is a consequence of the disruption of ATP supply in relation to membrane asymmetry?
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What technique is commonly used to demonstrate lateral heterogeneity in cell membranes?
What technique is commonly used to demonstrate lateral heterogeneity in cell membranes?
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What is the significance of phosphatidylinositol (PI) phosphorylation in membrane signaling?
What is the significance of phosphatidylinositol (PI) phosphorylation in membrane signaling?
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What happens to mitochondrial apoptogenic factors during cell death?
What happens to mitochondrial apoptogenic factors during cell death?
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How does lateral asymmetry in cell membranes contribute to cellular function?
How does lateral asymmetry in cell membranes contribute to cellular function?
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What is the significance of the lipid diacyl phosphatidylinositol dimannoside (Ac2PIM2) in the inner membrane?
What is the significance of the lipid diacyl phosphatidylinositol dimannoside (Ac2PIM2) in the inner membrane?
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Which components are primarily found in the main lipid classes of eukaryotic cell membranes?
Which components are primarily found in the main lipid classes of eukaryotic cell membranes?
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How does membrane asymmetry contribute to cellular function?
How does membrane asymmetry contribute to cellular function?
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What distinguishes prokaryotic cells from eukaryotic cells regarding membrane structure?
What distinguishes prokaryotic cells from eukaryotic cells regarding membrane structure?
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What role do lipid rafts play in biological membranes?
What role do lipid rafts play in biological membranes?
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Which of the following statements accurately describes the lipid composition of the outer leaflet of Gram-negative bacterial membranes?
Which of the following statements accurately describes the lipid composition of the outer leaflet of Gram-negative bacterial membranes?
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What indicates that mitochondria and plastids evolved from bacteria?
What indicates that mitochondria and plastids evolved from bacteria?
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Which characteristic of biological membranes affects their fluidity?
Which characteristic of biological membranes affects their fluidity?
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What effect does cholesterol have on the mobility of the hydrocarbon chains in phospholipid molecules?
What effect does cholesterol have on the mobility of the hydrocarbon chains in phospholipid molecules?
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Which statement correctly describes the role of phosphatidylserine (PS) in the cell membrane?
Which statement correctly describes the role of phosphatidylserine (PS) in the cell membrane?
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How does cholesterol affect phase transitions in the plasma membrane?
How does cholesterol affect phase transitions in the plasma membrane?
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What is the significance of membrane asymmetry with respect to inositol phospholipids (PI)?
What is the significance of membrane asymmetry with respect to inositol phospholipids (PI)?
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What effect does the negative charge of phosphatidylserine (PS) on the inner leaflet have?
What effect does the negative charge of phosphatidylserine (PS) on the inner leaflet have?
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Which of the following statements accurately describes the role of membrane proteins in maintaining membrane asymmetry?
Which of the following statements accurately describes the role of membrane proteins in maintaining membrane asymmetry?
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What is the principal reason why membranes are considered fluid structures?
What is the principal reason why membranes are considered fluid structures?
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According to the endosymbiotic theory, what is the primary origin of mitochondria and chloroplasts in eukaryotic cells?
According to the endosymbiotic theory, what is the primary origin of mitochondria and chloroplasts in eukaryotic cells?
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Which of the following statements best describes the significance of membrane asymmetry?
Which of the following statements best describes the significance of membrane asymmetry?
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What is the primary difference between eukaryotic and prokaryotic membranes?
What is the primary difference between eukaryotic and prokaryotic membranes?
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Which of the following is NOT a major component of biological membranes?
Which of the following is NOT a major component of biological membranes?
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Which of the following is a key feature of the fluid mosaic model of membrane structure?
Which of the following is a key feature of the fluid mosaic model of membrane structure?
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Which of the following statements accurately describes the role of transmembrane proteins in membrane function?
Which of the following statements accurately describes the role of transmembrane proteins in membrane function?
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Flashcards
Hydrophilic Properties
Hydrophilic Properties
Molecules that interact favorably with water due to polar groups; they easily dissolve in water.
Hydrophobic Properties
Hydrophobic Properties
Molecules that repel water and do not dissolve in it; often nonpolar.
Nernst Equation
Nernst Equation
Relates the concentration of ions across a membrane to its electrical potential.
Active Transport
Active Transport
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Chemiosmotic Theory
Chemiosmotic Theory
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Cholesterol in membranes
Cholesterol in membranes
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Membrane asymmetry
Membrane asymmetry
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Phosphatidylserine (PS)
Phosphatidylserine (PS)
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Inositol phospholipids (PI)
Inositol phospholipids (PI)
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Glycolipids
Glycolipids
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Membrane Composition
Membrane Composition
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Fluid Mosaic Model
Fluid Mosaic Model
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Transmembrane Proteins
Transmembrane Proteins
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Peripheral Proteins
Peripheral Proteins
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Permeability Barrier
Permeability Barrier
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Eukaryotic vs Prokaryotic Membranes
Eukaryotic vs Prokaryotic Membranes
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Membrane Protein Roles
Membrane Protein Roles
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Biological membranes
Biological membranes
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Phospholipids
Phospholipids
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Lipid rafts
Lipid rafts
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Cholesterol
Cholesterol
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Lipopolysaccharides (LPS)
Lipopolysaccharides (LPS)
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Endosymbiosis
Endosymbiosis
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Eukaryotic cell membrane composition
Eukaryotic cell membrane composition
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Light energy transduction
Light energy transduction
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Photophosphorylation
Photophosphorylation
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Chloroplasts
Chloroplasts
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Bioenergetics
Bioenergetics
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Molecular Biology of the Cell
Molecular Biology of the Cell
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Thermodynamics
Thermodynamics
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Biomembranes
Biomembranes
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Lipid organization
Lipid organization
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Phosphatidylserine (PS) role
Phosphatidylserine (PS) role
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Apoptotic cells
Apoptotic cells
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Asymmetry in cell membranes
Asymmetry in cell membranes
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Phosphatidylinositol (PI) signaling
Phosphatidylinositol (PI) signaling
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Downstream activation
Downstream activation
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Scramblase activity
Scramblase activity
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Fluorescence Recovery After Photobleaching (FRAP)
Fluorescence Recovery After Photobleaching (FRAP)
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Study Notes
BS332: Biomembranes and Bioenergetics
- The course consists of 17 lectures delivered by Professor Vass Bavro and Dr Dima Svistunenko
- A 2-hour revision workshop is scheduled
- Assessment is 100% based on a 3-hour summer exam scheduled in week 33-36
- Course materials are available on Moodle at https://moodle.essex.ac.uk/course/view.php?id=12353
- Recommended reading materials include "Bioenergetics 4" by David G. Nicholls and Stuart J. Ferguson, "Molecular Biology of the Cell" (various editions), "Brock Biology of Microorganisms", and "Atkins' Physical Chemistry"
- The course covers topics including membrane structure, lipid types, membrane protein function, thermodynamics, energy, and membrane transport.
Today's Lecture Topics
- Introductions to biomembranes and lipids
- Organization of prokaryotic and eukaryotic membranes
- Membrane components and their functions
- Membrane asymmetry
- Organization of bacterial and eukaryotic membranes
- Organelles and endosymbiosis theory
Learning Outcomes
- Students will be able to describe membrane components (proteins, polysaccharides, and lipids) and their functions
- Students will understand how proteins and lipids move within membranes
- Students will explain why membranes are fluid, membrane asymmetry, and its maintenance
- Students will describe the differences between eukaryotic and prokaryotic membranes
What are Membranes?
- Complex entities of lipids and proteins
- Some membranes contain water and sugar
- Fluid dynamic structures that comprise 50% of animal membrane mass
Why have Membranes?
- Permeability barriers maintaining cellular compartments
- Essential for biochemical reactions
- Allow the buildup of gradients (ions, protons, charge, and metabolites) enabling energy conversion
- Surround organelles providing specialized functions and compartmentalization
- Provide higher reagent concentration in enzymatic reactions for more efficient reactions
How Lipids Pack into Aqueous Environments
- Cone-shaped lipids form micelles
- Cylindrical phospholipids form bilayers
- Hydrophilic moieties interact with solution; hydrophobic tails bury within
The Bilayer
- Bilayer structure based on amphipathic phospholipids first proposed by Gorter and Grendel (1925)
- They observed that lipid extracted from erythrocytes has twice the surface area of the erythrocytes, suggesting a bilayer structure
- Danielli and Davson (1935) introduced the idea of proteins in membranes with their "trilamellar sandwich model"
- The fluid mosaic model of Singer and Nicolson (1972) provides the current understanding of membrane structure
Lipid plus Protein = Biomembrane
- Membrane proteins act as passive diffusion gates (controlled or not), ion channels, active transporters, and energy generators
- Proteins crucial for signaling, immune response, and defensive mechanisms
Basic Building Blocks - Lipids
- Lipids such as phospholipids and sphingolipids are crucial components for membrane structure and function
Diversity of Membrane Lipids
- Cells contain over 1000 different lipid species in eukaryotic cells including: glycerides, sphingolipids, phospholipids, cholesterol derivatives, and glycolipids
Main Membrane-Forming Lipids
- Phospholipids, including phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine, sphingomyelin, and others
- Glycolipids, including glucosylcerebrosides
- These phospholipids and glycolipids are crucial components of cellular membranes
The Phosphoglyceride Molecule
- A phospholipid (or glycerophospholipid)
- Has a glycerol backbone and two fatty acid tails
- Has a hydrophilic head group and hydrophobic tails
- Has different types of head groups like choline
- Saturated or unsaturated fatty acid chains have different kinds of impact on membrane fluidity
Main Lipids of Mammalian Membrane
- Some lipids can be charged (e.g., phosphatidylserine, pH-dependent negative charge)
Cholesterol Affects Diffusion
- Up to 1 molecule of cholesterol per phospholipid in eukaryotes
- Cholesterol interacts with the polar head groups and hydrocarbon chains of phospholipids, affecting membrane fluidity and permeability
Cholesterol in a Lipid Bilayer
- Cholesterol does not form a bilayer by itself
- Decreases the mobility of hydrocarbon chains in the phospholipids, enhancing membrane rigidity and reducing permeability
- Important in eukaryotic membranes at high concentrations
Membrane Asymmetry
- Phosphatidylserine (PS) is primarily found on the inner membrane leaflet
- Inositol phospholipids (PI) are concentrated in the inner membrane leaflet
- Glycolipids are primarily found on the outer membrane leaflet
- Inositol phospholipids involved in signaling
Asymmetry in Lipids is Functionally Significant
- Phosphatidylserine (PS) is a marker for cell status, important for coagulation cascade, and cell signaling.
- PS distribution used to distinguish living from dead/dying cells.
Phospholipid Translocators
- ER membranes are symmetric and randomized, with scramblase catalyzing flipping of phospholipids
- Plasma membranes (asymmetric), with flippase catalyzing specific phospholipids to the cytosolic monolayer.
Asymmetry in Lipids is Functionally Significant-Phosphatidyl Inositol Signaling
- A signal results in phosphorylation of phosphatidylinositol (PI)
- This recruits signaling molecules, resulting in downstream activation.
Membrane Asymmetry - Why is it Important?
- Unequal distribution of lipid components is a result of active transport
- Death program activation triggers the release of factors from the mitochondria
Asymmetry is not only across leaflets - lateral asymmetry creates mosaic of microdomains!
- Demonstration of lateral heterogeneity - Fluorescence Recovery After Photobleaching (FRAP)
Basic Organization of Eukaryotic vs Prokaryotic Membranes
- Eukaryotic membranes are more complex and diverse in lipid composition than prokaryotic membranes.
Membranes in the Eukaryotic Cell
- Eukaryotic cells contain internal membranes delimiting organelles (e.g., mitochondria, nucleus, endoplasmic reticulum, Golgi apparatus, lysosomes, peroxisomes)
Eukaryotic Membranes
- Lipid bilayers are the basic structure, with embedded proteins
- Contain 50% lipids and 50% proteins by mass
- Phospholipids, sphingolipids, glycerophospholipids (contain glycerol) are primary types
Critical Differences Between Bacterial and Eukaryotic Membranes
- Eukaryotic membranes contain a wide variety of phospholipids, while bacteria usually have fewer types.
- Bacteria can contain other types of lipids including ornithine lipids, sulfolipids, and hopanoids.
- Eukaryotic membranes do not contain LPS (lipopolysaccharides) or peptidoglycan.
- Bacterial cells lack sterols, instead containing hopanoids which support membrane structure
The Bacterial Cell Envelope
- Gram-positive and Gram-negative bacteria have distinct cell envelope structures
- Gram-positive cell walls have a thick peptidoglycan layer; Gram-negative walls have a thin peptidoglycan layer and an outer membrane containing LPS
The Gram-negative Cell Envelope
- OM (outer membrane) lipoproteins and proteins (e.g. porins, B-barrels)
- OM outer leaflet is lipopolysaccharides (LPS)
- This is important for bacterial defense mechanisms, and virulence factors
Summary of Membrane Organizations
- Different types of membranes of bacteria (Gram+, Gram-), mycobacteria, and fungi
Micobacteria Membranes
- Microbial membranes have complex organization, which is still being researched
Origin of Eukaryotic Organelles
- Endosymbiotic theory explains the origin of mitochondria and chloroplasts
- Mitochondria and plastids likely originated from bacteria that were engulfed and adopted into eukaryotic cells
Endosymbiosis - Mitochondria and Chloroplasts
- Diagrams showing the stages of endosymbiosis
Summary
- Biological membranes are lipid bilayers with embedded proteins, dynamic and semi-fluid structures.
- Membrane lipid composition varies among eukaryotes (phospholipids, cholesterol, glycolipids) and prokaryotes (variations on phospholipids and hopanoids).
- Lipid asymmetry is important for membrane function and regulation
- Prokaryotic cells generally lack membrane-bound organelles.
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Description
This quiz covers essential topics from the BS332 course on Biomembranes and Bioenergetics, including membrane structure, lipid types, and their functions. It also delves into energy dynamics and membrane transport mechanisms. Prepare to test your understanding of prokaryotic and eukaryotic membrane organization and asymmetry.