BS332: Biomembranes and Bioenergetics
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Questions and Answers

Which of the following statements about the Nernst equation is TRUE?

  • It only applies to situations where there is no active transport of ions across the membrane.
  • It predicts that the membrane potential will always be negative when the concentration of an ion is higher outside the cell.
  • It calculates the membrane potential across a membrane based on the concentration gradient of a single ion. (correct)
  • It considers the contribution of all ions present in the solution when calculating the membrane potential.
  • Which of the following is NOT a characteristic of active transport?

  • Requires energy input from ATP hydrolysis.
  • Movement of molecules against their concentration gradient.
  • Can be saturated with high substrate concentrations.
  • Always requires the presence of transmembrane proteins. (correct)
  • Which of the following statements accurately describes the role of F1F0-ATP synthase in oxidative phosphorylation?

  • It uses the proton gradient across the mitochondrial membrane to synthesize ATP. (correct)
  • It is involved in the transport of pyruvate into the mitochondria.
  • It directly transfers electrons from NADH to oxygen.
  • It catalyzes the breakdown of ATP into ADP and phosphate.
  • What is the primary driving force behind protein folding?

    <p>Hydrophobic interactions between nonpolar amino acids. (C)</p> Signup and view all the answers

    Which of the following processes is NOT directly coupled to the electron transport chain in mitochondria?

    <p>The oxidation of glucose to pyruvate. (B)</p> Signup and view all the answers

    What is primarily studied within the context of light energy transduction in chloroplasts?

    <p>Photosynthesis (D)</p> Signup and view all the answers

    Which of the following is a recommended reading that focuses on the principles of bioenergetics?

    <p>Bioenergetics (4th Edition) (A)</p> Signup and view all the answers

    Which edition of 'Molecular Biology of the Cell' is listed as a recommended reading resource?

    <p>6th Edition (B)</p> Signup and view all the answers

    What is the purpose of photophosphorylation within chloroplasts?

    <p>To produce ATP from ADP using light energy (D)</p> Signup and view all the answers

    Which source would provide lecture notes on thermodynamics?

    <p>Lecture Notes on Thermodynamics (D)</p> Signup and view all the answers

    Within metabolic studies, which chapter from 'Brock Biology of Microorganisms' is highlighted?

    <p>Chapter 13 (B)</p> Signup and view all the answers

    What aspect of membranes is described in the current lecture mentioned?

    <p>Organization of pro- and eukaryotic membranes (C)</p> Signup and view all the answers

    Which of the following is NOT an aspect of bioenergetics discussed in the recommended resources?

    <p>Chemical bonding in organic compounds (C)</p> Signup and view all the answers

    What role does the externalization of phosphatidylserine play in cells?

    <p>It signals for phagocytosis of apoptotic cells. (A)</p> Signup and view all the answers

    Which mechanism contributes to the maintenance of lipid asymmetry in the plasma membrane?

    <p>Active transport by phospholipid translocators. (C)</p> Signup and view all the answers

    What causes the randomization of phosphatidylserine in apoptotic cells?

    <p>Scramblase activity activation due to cell death signals. (B)</p> Signup and view all the answers

    What is a consequence of the disruption of ATP supply in relation to membrane asymmetry?

    <p>Inhibition of aminophospholipid translocase. (A)</p> Signup and view all the answers

    What technique is commonly used to demonstrate lateral heterogeneity in cell membranes?

    <p>Fluorescence recovery after photobleaching (FRAP). (D)</p> Signup and view all the answers

    What is the significance of phosphatidylinositol (PI) phosphorylation in membrane signaling?

    <p>It recruits signaling molecules leading to downstream activation. (C)</p> Signup and view all the answers

    What happens to mitochondrial apoptogenic factors during cell death?

    <p>They exit the mitochondria to activate scramblase activity. (B)</p> Signup and view all the answers

    How does lateral asymmetry in cell membranes contribute to cellular function?

    <p>It creates a mosaic of microdomains that facilitate specific interactions. (D)</p> Signup and view all the answers

    What is the significance of the lipid diacyl phosphatidylinositol dimannoside (Ac2PIM2) in the inner membrane?

    <p>It contributes to one-half of the hydrocarbon chains in the membrane. (B)</p> Signup and view all the answers

    Which components are primarily found in the main lipid classes of eukaryotic cell membranes?

    <p>Phospholipids, cholesterol, and glycolipids. (A)</p> Signup and view all the answers

    How does membrane asymmetry contribute to cellular function?

    <p>It is functionally significant for various cellular processes. (A)</p> Signup and view all the answers

    What distinguishes prokaryotic cells from eukaryotic cells regarding membrane structure?

    <p>Eukaryotic cells generally contain membrane organelles. (D)</p> Signup and view all the answers

    What role do lipid rafts play in biological membranes?

    <p>They are involved in the formation of functional domains. (D)</p> Signup and view all the answers

    Which of the following statements accurately describes the lipid composition of the outer leaflet of Gram-negative bacterial membranes?

    <p>It is composed primarily of lipopolysaccharides (LPS). (B)</p> Signup and view all the answers

    What indicates that mitochondria and plastids evolved from bacteria?

    <p>They share similarities with prokaryotic cell structures. (B)</p> Signup and view all the answers

    Which characteristic of biological membranes affects their fluidity?

    <p>The degree of unsaturation in fatty acid chains. (A)</p> Signup and view all the answers

    What effect does cholesterol have on the mobility of the hydrocarbon chains in phospholipid molecules?

    <p>It decreases their mobility making the lipid bilayer more rigid. (C)</p> Signup and view all the answers

    Which statement correctly describes the role of phosphatidylserine (PS) in the cell membrane?

    <p>It is concentrated in the inner layer and is crucial for the coagulation cascade. (C)</p> Signup and view all the answers

    How does cholesterol affect phase transitions in the plasma membrane?

    <p>It prevents hydrocarbon chains from coming together and inhibits crystallization. (D)</p> Signup and view all the answers

    What is the significance of membrane asymmetry with respect to inositol phospholipids (PI)?

    <p>They are primarily located in the inner leaflet, involved in signaling processes. (A)</p> Signup and view all the answers

    What effect does the negative charge of phosphatidylserine (PS) on the inner leaflet have?

    <p>It serves as a marker for cell status and activates protein kinases. (A)</p> Signup and view all the answers

    Which of the following statements accurately describes the role of membrane proteins in maintaining membrane asymmetry?

    <p>Membrane proteins act as anchors, tethering specific lipids to one leaflet of the membrane, thereby maintaining the asymmetrical distribution. (A)</p> Signup and view all the answers

    What is the principal reason why membranes are considered fluid structures?

    <p>The hydrophobic interactions between lipid tails allow for lateral movement within the membrane. (A)</p> Signup and view all the answers

    According to the endosymbiotic theory, what is the primary origin of mitochondria and chloroplasts in eukaryotic cells?

    <p>They were independently derived from ancestral prokaryotic cells that were engulfed by larger cells. (B)</p> Signup and view all the answers

    Which of the following statements best describes the significance of membrane asymmetry?

    <p>It allows for the creation of specialized compartments within the cell, facilitating distinct biochemical processes. (B)</p> Signup and view all the answers

    What is the primary difference between eukaryotic and prokaryotic membranes?

    <p>Eukaryotic membranes are more complex, containing a wider range of lipids and proteins compared to prokaryotic membranes. (D)</p> Signup and view all the answers

    Which of the following is NOT a major component of biological membranes?

    <p>Nucleic acids (A)</p> Signup and view all the answers

    Which of the following is a key feature of the fluid mosaic model of membrane structure?

    <p>Lipids and proteins can move laterally and rotate within the membrane, creating a dynamic structure. (A)</p> Signup and view all the answers

    Which of the following statements accurately describes the role of transmembrane proteins in membrane function?

    <p>Transmembrane proteins facilitate the transport of molecules across the membrane, regulating the movement of substances in and out of the cell. (A)</p> Signup and view all the answers

    Flashcards

    Hydrophilic Properties

    Molecules that interact favorably with water due to polar groups; they easily dissolve in water.

    Hydrophobic Properties

    Molecules that repel water and do not dissolve in it; often nonpolar.

    Nernst Equation

    Relates the concentration of ions across a membrane to its electrical potential.

    Active Transport

    The movement of ions or molecules across a membrane against their concentration gradient, requiring energy (ATP).

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    Chemiosmotic Theory

    Describes how ATP is produced using the proton gradient created by electron transport in mitochondria.

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    Cholesterol in membranes

    Cholesterol decreases permeability and adds rigidity to phospholipid bilayers.

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    Membrane asymmetry

    Membranes have uneven distribution of lipids, affecting function and charge.

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    Phosphatidylserine (PS)

    A negatively charged lipid found only in the inner leaflet of cell membranes.

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    Inositol phospholipids (PI)

    Phospholipids concentrated in the inner leaflet, playing key roles in signaling.

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    Glycolipids

    Lipids with sugar groups, located in the outer layer of the membrane.

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    Membrane Composition

    Membranes are primarily made of lipids, proteins, and polysaccharides.

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    Fluid Mosaic Model

    The model describing membranes as flexible structures with varied components.

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    Transmembrane Proteins

    Proteins that span the membrane and connect the inside and outside of the cell.

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    Peripheral Proteins

    Proteins that are attached to the membrane's surface, not embedded.

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    Permeability Barrier

    Membranes act as gatekeepers, controlling the entry and exit of substances.

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    Eukaryotic vs Prokaryotic Membranes

    Eukaryotic membranes are complex with organelles; prokaryotic membranes are simpler.

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    Membrane Protein Roles

    Membrane proteins perform functional activities, such as transport, signaling, or structural support.

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    Biological membranes

    Double layers of phospholipids with embedded proteins.

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    Phospholipids

    Main lipid class in cell membranes, forming bilayers.

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    Lipid rafts

    Microdomains in membranes with concentrated lipids and proteins.

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    Cholesterol

    A lipid that stabilizes cell membranes, found in animal cells.

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    Lipopolysaccharides (LPS)

    Components of the outer leaflet in Gram-negative bacterial membranes.

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    Endosymbiosis

    Theory that mitochondria and chloroplasts originated from bacteria.

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    Eukaryotic cell membrane composition

    Includes phospholipids, cholesterol, and glycolipids with diverse functions.

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    Light energy transduction

    The process by which light energy is converted into chemical energy in chloroplasts.

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    Photophosphorylation

    The process of generating ATP from ADP and inorganic phosphate using light energy.

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    Chloroplasts

    Organelles in plant cells where photosynthesis occurs, containing chlorophyll.

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    Bioenergetics

    The study of energy flow and conversion in biological systems.

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    Molecular Biology of the Cell

    A comprehensive textbook explaining cell structure and function at the molecular level.

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    Thermodynamics

    The branch of physics that studies the relationships between heat, work, and energy.

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    Biomembranes

    Thin structures that separate and protect cells, composed mainly of lipids and proteins.

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    Lipid organization

    The arrangement and behavior of lipids within a cell membrane, crucial for membrane function.

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    Phosphatidylserine (PS) role

    Used to distinguish between living and apoptotic cells.

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    Apoptotic cells

    Cells undergoing programmed cell death, exposing PS.

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    Asymmetry in cell membranes

    Refers to the unequal distribution of lipids in membranes.

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    Phosphatidylinositol (PI) signaling

    Phosphorylation of PI recruits signaling molecules.

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    Downstream activation

    The process following PI phosphorylation leading to cellular responses.

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    Scramblase activity

    Enzymatic activity responsible for lipid redistribution during apoptosis.

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    Fluorescence Recovery After Photobleaching (FRAP)

    Technique to study lateral heterogeneity in cell membranes.

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    Study Notes

    BS332: Biomembranes and Bioenergetics

    • The course consists of 17 lectures delivered by Professor Vass Bavro and Dr Dima Svistunenko
    • A 2-hour revision workshop is scheduled
    • Assessment is 100% based on a 3-hour summer exam scheduled in week 33-36
    • Course materials are available on Moodle at https://moodle.essex.ac.uk/course/view.php?id=12353
    • Recommended reading materials include "Bioenergetics 4" by David G. Nicholls and Stuart J. Ferguson, "Molecular Biology of the Cell" (various editions), "Brock Biology of Microorganisms", and "Atkins' Physical Chemistry"
    • The course covers topics including membrane structure, lipid types, membrane protein function, thermodynamics, energy, and membrane transport.

    Today's Lecture Topics

    • Introductions to biomembranes and lipids
    • Organization of prokaryotic and eukaryotic membranes
    • Membrane components and their functions
    • Membrane asymmetry
    • Organization of bacterial and eukaryotic membranes
    • Organelles and endosymbiosis theory

    Learning Outcomes

    • Students will be able to describe membrane components (proteins, polysaccharides, and lipids) and their functions
    • Students will understand how proteins and lipids move within membranes
    • Students will explain why membranes are fluid, membrane asymmetry, and its maintenance
    • Students will describe the differences between eukaryotic and prokaryotic membranes

    What are Membranes?

    • Complex entities of lipids and proteins
    • Some membranes contain water and sugar
    • Fluid dynamic structures that comprise 50% of animal membrane mass

    Why have Membranes?

    • Permeability barriers maintaining cellular compartments
    • Essential for biochemical reactions
    • Allow the buildup of gradients (ions, protons, charge, and metabolites) enabling energy conversion
    • Surround organelles providing specialized functions and compartmentalization
    • Provide higher reagent concentration in enzymatic reactions for more efficient reactions

    How Lipids Pack into Aqueous Environments

    • Cone-shaped lipids form micelles
    • Cylindrical phospholipids form bilayers
    • Hydrophilic moieties interact with solution; hydrophobic tails bury within

    The Bilayer

    • Bilayer structure based on amphipathic phospholipids first proposed by Gorter and Grendel (1925)
    • They observed that lipid extracted from erythrocytes has twice the surface area of the erythrocytes, suggesting a bilayer structure
    • Danielli and Davson (1935) introduced the idea of proteins in membranes with their "trilamellar sandwich model"
    • The fluid mosaic model of Singer and Nicolson (1972) provides the current understanding of membrane structure

    Lipid plus Protein = Biomembrane

    • Membrane proteins act as passive diffusion gates (controlled or not), ion channels, active transporters, and energy generators
    • Proteins crucial for signaling, immune response, and defensive mechanisms

    Basic Building Blocks - Lipids

    • Lipids such as phospholipids and sphingolipids are crucial components for membrane structure and function

    Diversity of Membrane Lipids

    • Cells contain over 1000 different lipid species in eukaryotic cells including: glycerides, sphingolipids, phospholipids, cholesterol derivatives, and glycolipids

    Main Membrane-Forming Lipids

    • Phospholipids, including phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine, sphingomyelin, and others
    • Glycolipids, including glucosylcerebrosides
    • These phospholipids and glycolipids are crucial components of cellular membranes

    The Phosphoglyceride Molecule

    • A phospholipid (or glycerophospholipid)
    • Has a glycerol backbone and two fatty acid tails
    • Has a hydrophilic head group and hydrophobic tails
    • Has different types of head groups like choline
    • Saturated or unsaturated fatty acid chains have different kinds of impact on membrane fluidity

    Main Lipids of Mammalian Membrane

    • Some lipids can be charged (e.g., phosphatidylserine, pH-dependent negative charge)

    Cholesterol Affects Diffusion

    • Up to 1 molecule of cholesterol per phospholipid in eukaryotes
    • Cholesterol interacts with the polar head groups and hydrocarbon chains of phospholipids, affecting membrane fluidity and permeability

    Cholesterol in a Lipid Bilayer

    • Cholesterol does not form a bilayer by itself
    • Decreases the mobility of hydrocarbon chains in the phospholipids, enhancing membrane rigidity and reducing permeability
    • Important in eukaryotic membranes at high concentrations

    Membrane Asymmetry

    • Phosphatidylserine (PS) is primarily found on the inner membrane leaflet
    • Inositol phospholipids (PI) are concentrated in the inner membrane leaflet
    • Glycolipids are primarily found on the outer membrane leaflet
    • Inositol phospholipids involved in signaling

    Asymmetry in Lipids is Functionally Significant

    • Phosphatidylserine (PS) is a marker for cell status, important for coagulation cascade, and cell signaling.
    • PS distribution used to distinguish living from dead/dying cells.

    Phospholipid Translocators

    • ER membranes are symmetric and randomized, with scramblase catalyzing flipping of phospholipids
    • Plasma membranes (asymmetric), with flippase catalyzing specific phospholipids to the cytosolic monolayer.

    Asymmetry in Lipids is Functionally Significant-Phosphatidyl Inositol Signaling

    • A signal results in phosphorylation of phosphatidylinositol (PI)
    • This recruits signaling molecules, resulting in downstream activation.

    Membrane Asymmetry - Why is it Important?

    • Unequal distribution of lipid components is a result of active transport
    • Death program activation triggers the release of factors from the mitochondria

    Asymmetry is not only across leaflets - lateral asymmetry creates mosaic of microdomains!

    • Demonstration of lateral heterogeneity - Fluorescence Recovery After Photobleaching (FRAP)

    Basic Organization of Eukaryotic vs Prokaryotic Membranes

    • Eukaryotic membranes are more complex and diverse in lipid composition than prokaryotic membranes.

    Membranes in the Eukaryotic Cell

    • Eukaryotic cells contain internal membranes delimiting organelles (e.g., mitochondria, nucleus, endoplasmic reticulum, Golgi apparatus, lysosomes, peroxisomes)

    Eukaryotic Membranes

    • Lipid bilayers are the basic structure, with embedded proteins
    • Contain 50% lipids and 50% proteins by mass
    • Phospholipids, sphingolipids, glycerophospholipids (contain glycerol) are primary types

    Critical Differences Between Bacterial and Eukaryotic Membranes

    • Eukaryotic membranes contain a wide variety of phospholipids, while bacteria usually have fewer types.
    • Bacteria can contain other types of lipids including ornithine lipids, sulfolipids, and hopanoids.
    • Eukaryotic membranes do not contain LPS (lipopolysaccharides) or peptidoglycan.
    • Bacterial cells lack sterols, instead containing hopanoids which support membrane structure

    The Bacterial Cell Envelope

    • Gram-positive and Gram-negative bacteria have distinct cell envelope structures
    • Gram-positive cell walls have a thick peptidoglycan layer; Gram-negative walls have a thin peptidoglycan layer and an outer membrane containing LPS

    The Gram-negative Cell Envelope

    • OM (outer membrane) lipoproteins and proteins (e.g. porins, B-barrels)
    • OM outer leaflet is lipopolysaccharides (LPS)
    • This is important for bacterial defense mechanisms, and virulence factors

    Summary of Membrane Organizations

    • Different types of membranes of bacteria (Gram+, Gram-), mycobacteria, and fungi

    Micobacteria Membranes

    • Microbial membranes have complex organization, which is still being researched

    Origin of Eukaryotic Organelles

    • Endosymbiotic theory explains the origin of mitochondria and chloroplasts
    • Mitochondria and plastids likely originated from bacteria that were engulfed and adopted into eukaryotic cells

    Endosymbiosis - Mitochondria and Chloroplasts

    • Diagrams showing the stages of endosymbiosis

    Summary

    • Biological membranes are lipid bilayers with embedded proteins, dynamic and semi-fluid structures.
    • Membrane lipid composition varies among eukaryotes (phospholipids, cholesterol, glycolipids) and prokaryotes (variations on phospholipids and hopanoids).
    • Lipid asymmetry is important for membrane function and regulation
    • Prokaryotic cells generally lack membrane-bound organelles.

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    Description

    This quiz covers essential topics from the BS332 course on Biomembranes and Bioenergetics, including membrane structure, lipid types, and their functions. It also delves into energy dynamics and membrane transport mechanisms. Prepare to test your understanding of prokaryotic and eukaryotic membrane organization and asymmetry.

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