Podcast
Questions and Answers
What is the primary effect of in-phase light waves in bright-field microscopy?
What is the primary effect of in-phase light waves in bright-field microscopy?
Reinforce each other, increasing brightness
How does the phase plate in phase contrast microscopy affect the waves?
How does the phase plate in phase contrast microscopy affect the waves?
It increases the phase difference to produce amplitude differences when waves recombine.
What is the purpose of the 1st barrier filter in fluorescence microscopy?
What is the purpose of the 1st barrier filter in fluorescence microscopy?
To filter the light before reaching the specimen and pass only wavelengths that excite specific fluorescence dyes.
Why is staining not necessary in phase contrast microscopy?
Why is staining not necessary in phase contrast microscopy?
What is the function of the objective lenses in a bright-field microscope?
What is the function of the objective lenses in a bright-field microscope?
How does fluorescence occur in fluorescence microscopy?
How does fluorescence occur in fluorescence microscopy?
What is the purpose of the condenser in a bright-field microscope?
What is the purpose of the condenser in a bright-field microscope?
Why is embedding in a block of wax or resin important in sample preparation?
Why is embedding in a block of wax or resin important in sample preparation?
What is the benefit of using immersion oil in microscopy?
What is the benefit of using immersion oil in microscopy?
How does phase contrast microscopy differ from bright-field microscopy in terms of sample preparation?
How does phase contrast microscopy differ from bright-field microscopy in terms of sample preparation?
