Bright-field Microscopy General Set-up

Reinforce each other, increasing brightness

It increases the phase difference to produce amplitude differences when waves recombine.

To filter the light before reaching the specimen and pass only wavelengths that excite specific fluorescence dyes.

<p>Because it works with phase differences to produce image contrast without the need for staining.</p> Signup and view all the answers

<p>To collect light from the sample and magnify the image for viewing.</p> Signup and view all the answers

<p>Fluorescence occurs when an electron returns to its ground state and emits light at a longer wavelength.</p> Signup and view all the answers

<p>To focus the light from the illumination source before it passes through the specimen.</p> Signup and view all the answers

<p>Embedding helps to provide support for the tissue during slicing with a microtome.</p> Signup and view all the answers

<p>Immersion oil increases resolving power and decreases the working distance of objective lenses.</p> Signup and view all the answers

<p>Phase contrast microscopy does not require staining or chemical changes to observe living and static cells.</p> Signup and view all the answers