Blood Smear & ABO-Rh Typing Quiz

Which of the following stains is commonly used for blood smears?

What is the primary purpose of using immersion oil in microscopy?

What is the purpose of the warming box in the staining process?

What is the purpose of alcohol preps when taking a blood sample?

What is the best way to dispose of used lancets?

Which reagents are used for ABO blood typing?

If a blood sample agglutinates with both anti-A and anti-B sera, what is the blood type?

What does agglutination with anti-D serum indicate?

What does the 'Rh' in ABO-Rh blood typing refer to?

What type of microscope is used to examine blood smears?

What do anti-A and anti-B sera contain?

If a blood sample does not agglutinate with any of the anti-A, anti-B, or anti-D sera, what is the blood type?

What are "Hype-Wipes" used for in a laboratory setting involving blood?

What is the purpose of using a gauze pad after a finger prick?

What is the purpose of fixing the blood smear (although not explicitly mentioned, it's an implied step)?

What is the correct order of steps related to slide preparation and viewing?

What does the absence of agglutination with all antisera (anti-A, anti-B, and anti-D) indicate?

The Ward's 36W0019 kit mentioned is a...

What is the definition of erythrocytes?

What is the definition of leukocytes?

What is the definition of platelets?

Platelets are not blood cells. They are cytoplasmic fragments, split from large cells called mega-karyocytes in the bone marrow.

Platelets have a dark-staining granular portion called a chromomere surrounded by a light-staining cytoplasmic portion called a Hyalomere.

The number of platelets in blood normally range from 150,000 to 350,000 per microliter of blood.

Platelets play several important roles in hemostasis.

Leukocytes are actually white; they appear translucent or colorless in an unstained preparation because they don't contain colored pigment as do erythrocytes.

In contrast to erythrocytes, all leukocytes contain a nucleus.

Leukocytes can be divided into two groups, based on the appearance of their cytoplasm.

Granulocytes contain large granules that are easily distinguished by the presence of large granules.

Agranulocytes contain cytoplasm that appears relatively homogenous or free of large granules.

Granulocytes consist of neutrophils, eosinophils, and basophils.

Granulocytes are produced in the bone marrow and range in size from 10 - 14 µm in diameter.

The cytoplasm of granulocytes contains large granules, mostly lysosomes, and their nuclei appear irregular and lobular, hence they are frequently called polymorphonuclear leukocytes.

Agranulocytes consist of lymphocytes and monocytes.

Lymphocytes and monocytes are produced in lymphatic tissue, such as lymph nodes and spleen.

Monocytes are produced in bone marrow.

The nucleus of lymphocytes and monocytes is generally spherical, and occasionally indented, giving rise to a horseshoe shape.

The amount of cytoplasm is reduced in agranulocytes because of its relatively large nucleus.

Leukocytes combat foreign substances that enter the body.

Leukocytes fight foreign substances in the body in the following ways: 1. Phagocytosis, 2. Take up toxins, 3. Release enzymes, 4. Produce antibodies and other important substances.

Erythrocytes are biconcave, disk shaped cells.

Erythrocytes have a diameter of approximately 8.5 µm and a thickness of 2.5 µm (near the edge of the cell).

The biconcave discoid shape of the erythrocyte is ideal because it allows the red cells to rapidly exchange CO2 and O2 with plasma.

Gas exchange, specifically the transport of oxygen and carbon dioxide in the blood is the erythrocyte's primary function.

Mature circulating erythrocytes do not have a nucleus.

About 35% of an erythrocyte is composed of a respiratory pigment and hemoglobin.

Hemoglobin, when bound with oxygen, imparts a red color to the cell.

The biconcave shape of an erythrocyte is attributed to tension of the cell membrane and the interaction with intracellular contents.

Abnormally shaped erythrocytes, such as sickle cells and microcytes, are frequently due to the presence of abnormal hemoglobin molecules or to an abnormal amount of hemoglobin.