Podcast
Questions and Answers
Why is it important that blood culture collection takes first priority in the order of draw?
Why is it important that blood culture collection takes first priority in the order of draw?
- To prevent contamination of the specimen. (correct)
- To comply with laboratory accreditation standards.
- To minimize patient discomfort during collection.
- To ensure accurate patient identification.
After selecting the venipuncture site, what is the recommended timeframe for releasing the tourniquet?
After selecting the venipuncture site, what is the recommended timeframe for releasing the tourniquet?
- Immediately after needle insertion.
- Within 60 seconds. (correct)
- After the last collection tube is filled.
- Within 30 seconds.
Why is it important to wait for 30 seconds after performing the friction rub with antiseptic solution before venipuncture?
Why is it important to wait for 30 seconds after performing the friction rub with antiseptic solution before venipuncture?
- To allow the antiseptic solution to fully penetrate and disinfect the site. (correct)
- To reduce the chance of hemolysis.
- To allow the patient to mentally prepare themselves for the procedure.
- To give the phlebotomist time to prepare the collection tubes.
When opening a culture bottle, what crucial step should be performed?
When opening a culture bottle, what crucial step should be performed?
Why is it important to check the fill lines on a blood culture bottle?
Why is it important to check the fill lines on a blood culture bottle?
After inoculating a blood culture bottle, what action should be taken to properly mix the blood with the medium?
After inoculating a blood culture bottle, what action should be taken to properly mix the blood with the medium?
What information should be included on the label of a blood culture specimen?
What information should be included on the label of a blood culture specimen?
Which of the following represents a proper method for media inoculation?
Which of the following represents a proper method for media inoculation?
What is the next recommended step after removing gloves after blood collection is completed?
What is the next recommended step after removing gloves after blood collection is completed?
Following collection, what is the appropriate action for transporting the blood culture specimen?
Following collection, what is the appropriate action for transporting the blood culture specimen?
Flashcards
Blood Culture Collection Priority
Blood Culture Collection Priority
Blood culture collection should take first priority in the order of draw to prevent contamination.
Venipuncture and Tourniquet
Venipuncture and Tourniquet
Select the venipuncture site, and release the tourniquet within 60 seconds.
Culture Bottle Inspection
Culture Bottle Inspection
Open the culture bottle by removing the flip-off cap, checking for defects, ensuring it draws at least 8 cc.
Media Inoculation Methods
Media Inoculation Methods
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Mixing Blood with Medium
Mixing Blood with Medium
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Study Notes
Blood Culture Collection Procedure
- Blood culture collection takes first priority in the order of draw to prevent contamination.
- Identify the patient properly and explain the collection procedure clearly.
- Select the venipuncture site and release the tourniquet within 60 seconds.
- Assemble the equipment and follow the proper aseptic technique.
- Perform the friction rub for about 60 second.
- Wait for 30 seconds to allow the site to dry.
- Open the culture bottle, removing the flip-off cap, while checking it for defects. The bottle should draw at least 8 cc.
- Clean the bottle stopper while waiting for the site to dry.
- Check the fill lines on the bottle to determine the minimum and maximum levels of the bottle.
- Reapply the tourniquet and perform the venipuncture, ensuring the site is not touched.
- Inoculate the medium.
- To mix the blood with the medium, invert the bottle a couple of times.
- If iodine is used in the arm, clean the patient's skin.
- Observe proper labeling procedures, including information about the site where the blood has been collected.
- Discard used and contaminated materials properly.
- Thank the patient courteously, remove gloves, and decontaminate hands with hand sanitizer.
- Transport the specimen promptly to the laboratory for analysis and processing.
Media Inoculation Methods
- Three ways to inoculate the medium:
- Directly into the bottle (during collection).
- Collected in a syringe (after collection).
- Through an intermediate collection tube (in the laboratory).
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