Blood Culture Collection Assistance
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Questions and Answers

What is the correct angle for inserting the needle into the vein?

  • 15° to 30° (correct)
  • 15° to 45°
  • 30° to 60°
  • 45° to 90°

    • Why is it important to have the patient clench their fist?

  • To help locate the vein
  • To stretch the skin downward (correct)
  • To make the patient more comfortable
  • To increase blood flow to the vein

    • What is the correct order of draw for anaerobic and aerobic blood cultures?

  • Anaerobic first, then aerobic (correct)
  • It doesn't matter which one is first
  • Aerobic first, then anaerobic
  • Only collect one or the other

    • Why is it important to invert each tube after collection?

    <p>To mix the specimen properly</p> Signup and view all the answers

    What is the purpose of the two-point check?

    <p>To check for bleeding at the puncture site</p> Signup and view all the answers

    When should the tourniquet be removed?

    <p>Before 1 minute</p> Signup and view all the answers

    What is the correct way to dispose of the needle assembly?

    <p>In a sharps container</p> Signup and view all the answers

    Why is it important to apply pressure to the puncture site?

    <p>To stop bleeding</p> Signup and view all the answers

    What is the final step in the blood collection procedure?

    <p>Leaving the room or dismissing the patient</p> Signup and view all the answers

    What is the purpose of stretching the skin downward below the collection site?

    <p>To anchor the vein in place</p> Signup and view all the answers

    What should be done when collecting two blood cultures from the same patient?

    <p>Use new supplies for both blood culture collections.</p> Signup and view all the answers

    What is an essential step in preparing the patient's skin for blood culture collection?

    <p>Cleansing the area twice with at least two antiseptic wipes.</p> Signup and view all the answers

    What should be done with the blood culture specimens after collection?

    <p>Take them to the laboratory immediately.</p> Signup and view all the answers

    What is a critical aspect of observing standard precautions when assisting with blood cultures?

    <p>Providing gloves for the person being assisted and wearing gloves as well.</p> Signup and view all the answers

    What should be done to ensure proper disposal of supplies after blood culture collection?

    <p>Assist with proper disposal of all supplies.</p> Signup and view all the answers

    What should be documented after assisting with blood culture collection?

    <p>The procedure, date, time, and who performed it.</p> Signup and view all the answers

    Why is it important to have two complete sets of equipment for blood culture collection?

    <p>To prevent contamination and ensure accurate results.</p> Signup and view all the answers

    What should be done to ensure the patient's safety after blood culture collection?

    <p>Apply pressure bandages to the venipuncture sites and check for bleeding.</p> Signup and view all the answers

    What is the purpose of having a protective pad under the patient's arm during blood culture collection?

    <p>To protect the patient's clothing from stains.</p> Signup and view all the answers

    What should be ensured when preparing for blood culture collection?

    <p>Two blood culture bottles are available, one aerobic and one anaerobic.</p> Signup and view all the answers

    What is the primary purpose of ordering a blood culture test?

    <p>To diagnose conditions in patients with a fever of unknown origin (FUO)</p> Signup and view all the answers

    What is the minimum number of patient identifiers required to properly identify a patient before collecting a blood culture sample?

    <p>Two</p> Signup and view all the answers

    What is the purpose of washing hands and donning gloves before collecting a blood culture sample?

    <p>To prevent infection transmission to the patient</p> Signup and view all the answers

    How many blood culture bottles are required per collection?

    <p>One aerobic and one anaerobic</p> Signup and view all the answers

    What is the purpose of applying a tourniquet during blood culture collection?

    <p>To make the vein more accessible</p> Signup and view all the answers

    What is the recommended method for cleansing the skin before venipuncture?

    <p>70% isopropyl alcohol with a friction scrub</p> Signup and view all the answers

    What should be done after cleansing the skin with 70% isopropyl alcohol?

    <p>Allow the area to dry completely</p> Signup and view all the answers

    What is the purpose of reviewing the medical requisition form before collecting a blood culture sample?

    <p>To determine how much blood needs to be collected</p> Signup and view all the answers

    What happens to the blood sample after it is sent to the laboratory?

    <p>It is placed into a specialized dish and observed for micro-organism growth</p> Signup and view all the answers

    What is the next step if micro-organisms are found to be growing in the blood culture sample?

    <p>Further tests are done to identify the specific micro-organisms</p> Signup and view all the answers

    Antiseptic pads can be reused when collecting multiple blood cultures from the same patient.

    <p>False</p> Signup and view all the answers

    When assisting with blood cultures, it is necessary to provide gloves for the person you are assisting, but not for yourself.

    <p>False</p> Signup and view all the answers

    Only one blood culture bottle is required per collection, regardless of the type of culture.

    <p>False</p> Signup and view all the answers

    When collecting blood cultures, you should wash your hands before the procedure, but gloves are not necessary.

    <p>False</p> Signup and view all the answers

    After collecting blood cultures, the specimens can be left at room temperature for several hours before being sent to the laboratory.

    <p>False</p> Signup and view all the answers

    The area should be touched after it has been cleansed and allowed to dry completely.

    <p>False</p> Signup and view all the answers

    The needle should be inserted into the vein at a 90° angle.

    <p>False</p> Signup and view all the answers

    The patient should be asked to unclench their fist after the blood culture bottle is filled.

    <p>False</p> Signup and view all the answers

    The tourniquet should be removed after 2 minutes.

    <p>False</p> Signup and view all the answers

    The needle assembly should be disposed of in a regular trash can.

    <p>False</p> Signup and view all the answers

    The antiseptic used to cleanse the top of the blood collection bottles is povidone-iodine.

    <p>False</p> Signup and view all the answers

    A blood culture requisition is typically ordered to diagnose patients with a known fever origin.

    <p>False</p> Signup and view all the answers

    The tourniquet should be left on until the needle is inserted into the vein.

    <p>False</p> Signup and view all the answers

    Only one blood culture bottle is required per collection.

    <p>False</p> Signup and view all the answers

    If micro-organisms are found to be growing in the blood culture sample, the test is complete and no further testing is needed.

    <p>False</p> Signup and view all the answers

    Study Notes

    Assisting with Specimen Collections

    • Other health professionals may need assistance with blood culture collections, which involves notifying laboratory personnel, assembling materials, and explaining procedures to patients.
    • When collecting two blood cultures from the same patient, ensure two complete sets of equipment are available, and do not reuse antiseptic pads, gauze, or needles.

    Blood Culture Collection Procedure

    • Obtain and label proper tubes or bottles.
    • Wash hands, and put on gloves, as well as provide gloves for the person assisting.
    • Place a protective pad under the patient's arm.
    • Prepare the patient's skin with at least two antiseptic wipes and cleanse the area twice.
    • Assist in the venipuncture process.
    • Assist with applying pressure bandages to venipuncture sites upon completion.
    • Check the patient a few minutes later to ensure bleeding has stopped.
    • Assist with proper disposal of all supplies.
    • Document the procedure, including date, time, and who performed it.
    • Immediately take the specimens to the laboratory.

    Blood Cultures

    • Blood cultures are laboratory tests used to check for bacteria or microorganisms in a blood sample.
    • They are used to diagnose conditions in patients with a fever of unknown origin (FUO).
    • When processing a blood sample, it is placed into a specialized dish and observed to see if microorganisms grow.
    • If growth occurs, further tests are done to identify the specific microorganisms present.

    Steps to Perform a Blood Culture

    • Introduce yourself and properly identify the patient using at least two patient identifiers.
    • Wash hands, and put on gloves.
    • Assemble supplies, including a winged infusion set or syringe needle, adapter, and blood culture collection bottles.
    • Review the medical requisition form to determine how much blood needs to be collected.
    • Mark blood collection bottles with the required blood level.
    • Remove the protective cap, and cleanse the top of the blood collection bottles with an antiseptic.
    • Apply a tourniquet and palpate to find an accessible blood vessel.
    • Cleanse the area with 70% isopropyl alcohol with a friction scrub, followed by chlorhexidine gluconate or povidone-iodine.
    • Allow the area to dry completely before venipuncture.
    • Reapply the tourniquet after the area has dried.
    • Insert the needle into the vein at a 15° to 30° angle with the bevel facing up.
    • Pop the blood culture bottle onto the double-pointed needle.
    • Have the patient unclench their fist as blood enters the tube or bottle.
    • Gently invert the tube to mix the specimen after removal from the needle holder.
    • Remove the tourniquet before 1 minute and monitor the patient's condition.
    • Dispose of the needle assembly into a sharps container and apply pressure to the puncture site.
    • Label all blood collection bottles and tubes before leaving the patient.
    • Check the patient's status again before leaving the room or dismissing the patient.

    Assisting with Specimen Collections

    • Other health professionals may need assistance with blood culture collections, which involves notifying laboratory personnel, assembling materials, and explaining procedures to patients.
    • When collecting two blood cultures from the same patient, ensure two complete sets of equipment are available, and do not reuse antiseptic pads, gauze, or needles.

    Blood Culture Collection Procedure

    • Obtain and label proper tubes or bottles.
    • Wash hands, and put on gloves, as well as provide gloves for the person assisting.
    • Place a protective pad under the patient's arm.
    • Prepare the patient's skin with at least two antiseptic wipes and cleanse the area twice.
    • Assist in the venipuncture process.
    • Assist with applying pressure bandages to venipuncture sites upon completion.
    • Check the patient a few minutes later to ensure bleeding has stopped.
    • Assist with proper disposal of all supplies.
    • Document the procedure, including date, time, and who performed it.
    • Immediately take the specimens to the laboratory.

    Blood Cultures

    • Blood cultures are laboratory tests used to check for bacteria or microorganisms in a blood sample.
    • They are used to diagnose conditions in patients with a fever of unknown origin (FUO).
    • When processing a blood sample, it is placed into a specialized dish and observed to see if microorganisms grow.
    • If growth occurs, further tests are done to identify the specific microorganisms present.

    Steps to Perform a Blood Culture

    • Introduce yourself and properly identify the patient using at least two patient identifiers.
    • Wash hands, and put on gloves.
    • Assemble supplies, including a winged infusion set or syringe needle, adapter, and blood culture collection bottles.
    • Review the medical requisition form to determine how much blood needs to be collected.
    • Mark blood collection bottles with the required blood level.
    • Remove the protective cap, and cleanse the top of the blood collection bottles with an antiseptic.
    • Apply a tourniquet and palpate to find an accessible blood vessel.
    • Cleanse the area with 70% isopropyl alcohol with a friction scrub, followed by chlorhexidine gluconate or povidone-iodine.
    • Allow the area to dry completely before venipuncture.
    • Reapply the tourniquet after the area has dried.
    • Insert the needle into the vein at a 15° to 30° angle with the bevel facing up.
    • Pop the blood culture bottle onto the double-pointed needle.
    • Have the patient unclench their fist as blood enters the tube or bottle.
    • Gently invert the tube to mix the specimen after removal from the needle holder.
    • Remove the tourniquet before 1 minute and monitor the patient's condition.
    • Dispose of the needle assembly into a sharps container and apply pressure to the puncture site.
    • Label all blood collection bottles and tubes before leaving the patient.
    • Check the patient's status again before leaving the room or dismissing the patient.

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    c5-BLOOD CULTURES.docx

    Description

    Learn how to assist other health professionals with blood culture collections, including preparing materials and explaining procedures to patients.

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