Podcast
Questions and Answers
What is the first step to prepare the finger for a blood sample puncture?
What is the first step to prepare the finger for a blood sample puncture?
What should be done after puncturing the finger and obtaining the first drop of blood?
What should be done after puncturing the finger and obtaining the first drop of blood?
What angle should the spreading slide be held at while making the blood smear?
What angle should the spreading slide be held at while making the blood smear?
How long should the Leishman's stain be allowed to act on the blood film?
How long should the Leishman's stain be allowed to act on the blood film?
Signup and view all the answers
What is the purpose of adding an equivalent amount of distilled water to the stain on the slide?
What is the purpose of adding an equivalent amount of distilled water to the stain on the slide?
Signup and view all the answers
What must be avoided when placing the blood drop on the clean slide?
What must be avoided when placing the blood drop on the clean slide?
Signup and view all the answers
What is the final step before examining the blood smear under an oil immersion lens?
What is the final step before examining the blood smear under an oil immersion lens?
Signup and view all the answers
What is the primary function of red blood cells?
What is the primary function of red blood cells?
Signup and view all the answers
Which type of white blood cell makes up the largest percentage of total WBCs?
Which type of white blood cell makes up the largest percentage of total WBCs?
Signup and view all the answers
What percentage of blood volume do platelets constitute?
What percentage of blood volume do platelets constitute?
Signup and view all the answers
What is the main component of plasma?
What is the main component of plasma?
Signup and view all the answers
Which leukocyte classification lacks granules in their cytoplasm?
Which leukocyte classification lacks granules in their cytoplasm?
Signup and view all the answers
Which staining method is used in the examination of a blood film?
Which staining method is used in the examination of a blood film?
Signup and view all the answers
What is the average lifespan of a red blood cell?
What is the average lifespan of a red blood cell?
Signup and view all the answers
What is one of the functions of blood in the body?
What is one of the functions of blood in the body?
Signup and view all the answers
Study Notes
Blood Composition and Function
- Blood is a specialized body fluid with diverse functions, including transporting oxygen and nutrients, carrying infection-fighting cells, removing waste, and regulating temperature.
- Whole blood is a mixture of approximately 55% plasma and 45% blood cells. A significant portion (7-8%) of a person's total body weight is blood.
- Blood is a highly specialized tissue consisting of over 4000 components.
- The most crucial components are red blood cells, white blood cells, platelets, and plasma.
Red Blood Cells (RBCs)
- Also known as erythrocytes, these are flattened, bi-concave cells.
- RBCs transport oxygen.
- A typical count ranges from 4 to 6 million cells per cubic millimeter of blood.
- RBCs are produced continuously in the bone marrow.
- Each RBC lives approximately 120 days before being destroyed in the liver or spleen.
White Blood Cells (WBCs)
- Also called leukocytes, WBCs are crucial to the immune system.
- Various types of WBCs exist, categorized as granulocytes and agranulocytes.
Platelets
- Also called thrombocytes, platelets are essential for blood clotting.
- They constitute less than 1% of blood volume.
Plasma
- Plasma is the liquid portion of blood, primarily 90% water.
- It contains dissolved materials like proteins, glucose, ions, hormones, and gases.
- Plasma acts as a buffer, maintaining a pH near 7.4
Blood Film Preparation (Experiment)
- The procedure typically involves obtaining a blood sample via finger puncture.
- A thin blood film (smear) is created by spreading a small blood droplet on a glass slide.
- The film is stained using Leishman's stain. This stain has components (eosin and methylene blue) that target certain aspects of the cells and their components, enabling differentiation.
- The stained film is examined under a microscope.
Types of Leukocytes
- Granulocytes (including neutrophils, basophils, and eosinophils) have granules in their cytoplasm.
- Polymorphonuclear cells have multiple lobes in their nuclei.
- Agranulocytes (including monocytes and lymphocytes) lack cytoplasmic granules.
- Monocytes and lymphocytes are mononuclear.
- Neutrophils are the most abundant type of WBC (60-70%).
- Basophils are less than 1% of WBCs.
- Eosinophils account for 2-4% of WBCs.
- Monocytes make up 3-8% of WBCs.
- Lymphocytes contribute 20% of WBCs.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Related Documents
Description
Test your knowledge on the composition and functions of blood. This quiz covers the different components of blood, including red and white blood cells, and their essential roles in the body. Challenge yourself to see how well you understand this vital fluid.