Blood Composition and Function Quiz
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Questions and Answers

What is the first step to prepare the finger for a blood sample puncture?

  • Apply Leishman's stain
  • Clean the glass slide
  • Rub the finger to allow more blood to come out (correct)
  • Use a clean gauze to dry the finger
  • What should be done after puncturing the finger and obtaining the first drop of blood?

  • Put the finger in sanitizer
  • Immediately place the drop on the staining rack
  • Cover the slide with sufficient Leishman's stain
  • Remove the first drop of blood with dry gauze (correct)
  • What angle should the spreading slide be held at while making the blood smear?

  • 60°
  • 45° (correct)
  • 90°
  • How long should the Leishman's stain be allowed to act on the blood film?

    <p>1 minute</p> Signup and view all the answers

    What is the purpose of adding an equivalent amount of distilled water to the stain on the slide?

    <p>To dilute the stain for better visibility</p> Signup and view all the answers

    What must be avoided when placing the blood drop on the clean slide?

    <p>Touching the slide with the finger skin</p> Signup and view all the answers

    What is the final step before examining the blood smear under an oil immersion lens?

    <p>Shake the slide gently in the air</p> Signup and view all the answers

    What is the primary function of red blood cells?

    <p>Transporting oxygen to the tissues</p> Signup and view all the answers

    Which type of white blood cell makes up the largest percentage of total WBCs?

    <p>Neutrophils</p> Signup and view all the answers

    What percentage of blood volume do platelets constitute?

    <p>1%</p> Signup and view all the answers

    What is the main component of plasma?

    <p>Water</p> Signup and view all the answers

    Which leukocyte classification lacks granules in their cytoplasm?

    <p>Monocytes</p> Signup and view all the answers

    Which staining method is used in the examination of a blood film?

    <p>Leishman's stain</p> Signup and view all the answers

    What is the average lifespan of a red blood cell?

    <p>120 days</p> Signup and view all the answers

    What is one of the functions of blood in the body?

    <p>Regulating body temperature</p> Signup and view all the answers

    Study Notes

    Blood Composition and Function

    • Blood is a specialized body fluid with diverse functions, including transporting oxygen and nutrients, carrying infection-fighting cells, removing waste, and regulating temperature.
    • Whole blood is a mixture of approximately 55% plasma and 45% blood cells. A significant portion (7-8%) of a person's total body weight is blood.
    • Blood is a highly specialized tissue consisting of over 4000 components.
    • The most crucial components are red blood cells, white blood cells, platelets, and plasma.

    Red Blood Cells (RBCs)

    • Also known as erythrocytes, these are flattened, bi-concave cells.
    • RBCs transport oxygen.
    • A typical count ranges from 4 to 6 million cells per cubic millimeter of blood.
    • RBCs are produced continuously in the bone marrow.
    • Each RBC lives approximately 120 days before being destroyed in the liver or spleen.

    White Blood Cells (WBCs)

    • Also called leukocytes, WBCs are crucial to the immune system.
    • Various types of WBCs exist, categorized as granulocytes and agranulocytes.

    Platelets

    • Also called thrombocytes, platelets are essential for blood clotting.
    • They constitute less than 1% of blood volume.

    Plasma

    • Plasma is the liquid portion of blood, primarily 90% water.
    • It contains dissolved materials like proteins, glucose, ions, hormones, and gases.
    • Plasma acts as a buffer, maintaining a pH near 7.4

    Blood Film Preparation (Experiment)

    • The procedure typically involves obtaining a blood sample via finger puncture.
    • A thin blood film (smear) is created by spreading a small blood droplet on a glass slide.
    • The film is stained using Leishman's stain. This stain has components (eosin and methylene blue) that target certain aspects of the cells and their components, enabling differentiation.
    • The stained film is examined under a microscope.

    Types of Leukocytes

    • Granulocytes (including neutrophils, basophils, and eosinophils) have granules in their cytoplasm.
    • Polymorphonuclear cells have multiple lobes in their nuclei.
    • Agranulocytes (including monocytes and lymphocytes) lack cytoplasmic granules.
    • Monocytes and lymphocytes are mononuclear.
    • Neutrophils are the most abundant type of WBC (60-70%).
    • Basophils are less than 1% of WBCs.
    • Eosinophils account for 2-4% of WBCs.
    • Monocytes make up 3-8% of WBCs.
    • Lymphocytes contribute 20% of WBCs.

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    Related Documents

    Blood Film PDF

    Description

    Test your knowledge on the composition and functions of blood. This quiz covers the different components of blood, including red and white blood cells, and their essential roles in the body. Challenge yourself to see how well you understand this vital fluid.

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