Untitled Quiz

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson
Download our mobile app to listen on the go
Get App

Questions and Answers

What is the main consequence of lactic acidosis in the blood?

  • Accumulation of lactic acid. (correct)
  • Decrease in blood lactate levels.
  • Increase in blood pH.
  • Enhanced oxygen delivery to tissues.

What genetic defect contributes to inadequate oxygen supply to tissues?

  • Pyruvate kinase deficiency.
  • Glucose-6-phosphate dehydrogenase deficiency.
  • Lactate dehydrogenase deficiency.
  • Hexokinase deficiency. (correct)

How is pyruvate converted to acetyl-CoA?

  • By reduction in the nucleus.
  • By hydrolysis in the mitochondria.
  • By fermentation in the cytoplasm.
  • By oxidative decarboxylation in the mitochondria. (correct)

What is generated during the conversion of one molecule of pyruvate to acetyl-CoA?

<p>One NADH and no ATP. (A)</p> Signup and view all the answers

What is a key product of the citric acid cycle?

<p>NADH. (A)</p> Signup and view all the answers

What is the total amount of ATP generated from one mole of acetyl-CoA in the citric acid cycle?

<p>10 ATP. (A)</p> Signup and view all the answers

Which hormone primarily regulates the rate of the citric acid cycle?

<p>ATP concentration. (D)</p> Signup and view all the answers

What is the dual function of the citric acid cycle described as?

<p>Amphibolic process. (A)</p> Signup and view all the answers

What is the effect of increased NADPH concentration on G-6-PD activity?

<p>Decreases G-6-PD activity (D)</p> Signup and view all the answers

What genetic disorder is associated with a deficiency in G-6-PD?

<p>Hemolytic anemia (C)</p> Signup and view all the answers

Which enzyme's reduced activity is directly linked to Wernicke-Korsakoff syndrome?

<p>Transketolase (D)</p> Signup and view all the answers

What is the primary function of the uronic acid pathway?

<p>To convert glucose to glucuronic acid (A)</p> Signup and view all the answers

In individuals with G-6-PD deficiency, exposure to specific drugs can lead to which condition?

<p>Hemolytic anemia (B)</p> Signup and view all the answers

What is the role of UDP-glucuronate in the liver?

<p>Acts as a precursor in the synthesis of glycoproteins (A)</p> Signup and view all the answers

Which of the following is NOT a symptom of Wernicke-Korsakoff syndrome?

<p>Excessive thirst (A)</p> Signup and view all the answers

What effect does insulin have on the pentose phosphate pathway?

<p>Enhances the pathway by inducing specific enzymes (C)</p> Signup and view all the answers

What is a primary consequence of lactose fermentation in the intestines?

<p>Production of carbon dioxide and methane gases (D)</p> Signup and view all the answers

What happens to D-glucose after absorption in the intestine?

<p>Most of it is phosphorylated to glucose-6-phosphate in the liver. (D)</p> Signup and view all the answers

During which phase of glycolysis is energy consumed?

<p>Energy-requiring phase (C)</p> Signup and view all the answers

Which metabolic pathway is glucose-6-phosphate NOT associated with?

<p>TCA cycle (D)</p> Signup and view all the answers

What is one role of 2,3-BPG produced in the Rapoport Lubering cycle?

<p>It enhances the release of oxygen from hemoglobin. (C)</p> Signup and view all the answers

What is a typical symptom of lactose intolerance?

<p>Abdominal cramps and flatulence (D)</p> Signup and view all the answers

How does anaerobic glycolysis differ from aerobic glycolysis?

<p>Anaerobic glycolysis produces less ATP than aerobic glycolysis. (D)</p> Signup and view all the answers

What occurs during the Rapoport Lubering cycle specifically in erythrocytes?

<p>There is no net production of ATP. (C)</p> Signup and view all the answers

Flashcards

Lactose Fermentation

Bacterial breakdown of lactose, creating gases (H2, CO2, methane) and acids (acetic, propionic, butyric).

Lactose Intolerance Symptoms

Abdominal discomfort from gas buildup and osmotic pressure caused by undigested lactose, which draws water into intestines leading to diarrhea and dehydration.

Glycolysis Location

Glycolysis happens in the cell's cytoplasm.

Glycolysis Definition

Breakdown of glucose into pyruvate, yielding energy (ATP) in the presence or absence of oxygen.

Signup and view all the flashcards

Anaerobic Glycolysis

Formation of lactate from pyruvate during oxygen deprivation; a backup energy source for some tissues.

Signup and view all the flashcards

Rapoport-Luebering Cycle

A pathway in red blood cells that generates 2,3-bisphosphoglycerate (2,3-BPG), which regulates hemoglobin's oxygen binding.

Signup and view all the flashcards

2,3-BPG Role

2,3-BPG is crucial for oxygen release from hemoglobin in red blood cells; a reaction buffer and regulator.

Signup and view all the flashcards

Glycolysis Significance

Essential for producing ATP (energy) from glucose; the primary pathway of glucose metabolism.

Signup and view all the flashcards

Lactic Acidosis

Accumulation of lactic acid in the blood, lowering blood pH.

Signup and view all the flashcards

Hexokinase Deficiency

Genetic problem affecting erythrocyte hexokinase, leading to low 2,3-BPG and anemia.

Signup and view all the flashcards

Pyruvate to Acetyl-CoA

Conversion of pyruvate to Acetyl CoA, an irreversible reaction in mitochondria.

Signup and view all the flashcards

Citric Acid Cycle

A series of reactions that breaks down Acetyl-CoA to produce ATP and water in mitochondria

Signup and view all the flashcards

Citric Acid Cycle Energetics

Produces 3 NADH, 1 FADH2, and 1 ATP per Acetyl-CoA.

Signup and view all the flashcards

Citric Acid Cycle Significance

The cycle is crucial in energy production and is a common pathway for the breakdown of carbs, fats, and proteins.

Signup and view all the flashcards

Citric Acid Cycle Regulation

Rate regulated by ATP, NADH, and Acetyl-CoA levels to maintain energy balance

Signup and view all the flashcards

Pyruvate Dehydrogenase (PDH) Coenzyme Requirement

PDH, the multienzyme complex that converts pyruvate to Acetyl-CoA, needs five coenzymes(TPP, lipoate, CoA, FAD, NAD+)

Signup and view all the flashcards

UDP-glucuronate & Importance

UDP-glucuronate is a crucial molecule produced by the uronic acid pathway. It's essential for synthesizing glycosaminoglycans, glycoproteins, and plays a role in detoxification.

Signup and view all the flashcards

Study Notes

Carbohydrate Metabolism

  • Dietary carbohydrates consist of polysaccharides (starch, glycogen, cellulose), disaccharides (sucrose, maltose, lactose), monosaccharides (glucose, fructose).

Digestion in Mouth

  • Salivary glands secrete α-amylase.
  • α-amylase acts briefly on dietary starch and glycogen, breaking some α-(1 → 4) bonds.
  • α-amylase hydrolyzes starch into dextrins.

Digestion in Stomach

  • Carbohydrate digestion halts temporarily in the stomach.

Digestion in Intestine

  • Two phases of intestinal digestion:

    • Digestion due to pancreatic α-amylase:
      • Pancreatic α-amylase further degrades dextrins into a mixture of maltose, isomaltose, and α-limit dextrin.
      • α-limit dextrins are smaller oligosaccharides containing 3 to 5 glucose units.
    • Digestion due to intestinal enzymes:
      • Sucrose is broken down by sucrase into glucose and fructose.
      • Maltose is broken down by maltase into two glucose molecules.
      • Lactose is broken down by lactase into glucose and galactose.
      • Isomaltose is broken down by isomaltase into two glucose molecules.
      • α-limit dextrin is broken down by dextrinase into glucose and maltose.
  • The end products of carbohydrate digestion are glucose, fructose, and galactose.

Absorption of Carbohydrates

  • Two mechanisms are responsible for monosaccharide absorption:
    • Active transport (against concentration gradient):
      • Used for glucose and galactose transport from lower to higher concentration.
      • Requires energy and a specific transport protein.
      • Involves sodium ions.
    • Facilitative transport (with concentration gradient):
      • Used for fructose and mannose transport from higher to lower concentration.
      • Doesn't require energy, but requires a specific transporter (GLUT-5).
  • Sugars are transported out of mucosal cells via GLUT-2 and into the bloodstream.

Lactose Intolerance

  • Inability to digest lactose (milk sugar) due to lactase deficiency.
  • Lactose undergoes bacterial fermentation, producing gases (H2, CO2, methane), and organic acids (acetic, propionic, butyric acids).
  • Symptoms include abdominal cramps and flatulence, diarrhea, and dehydration.
  • Treatment involves removing lactose from the diet.

Metabolic Fate of Carbohydrates

  • Monosaccharides are transported to the liver via the portal circulation.
  • Most glucose is phosphorylated to glucose-6-phosphate, trapped in the liver cell, and cannot diffuse back out.
  • The rest of the glucose enters the bloodstream.
  • Other monosaccharides (galactose and fructose) are also phosphorylated and converted into glucose.

Glycolysis

  • Glycolysis (Embden-Meyerhof pathway) is the sequence of reactions that converts glucose to pyruvate in the presence of oxygen (aerobic) or lactate in the absence of oxygen (anaerobic).
  • Produces ATP.
  • It takes place in the cytosol of all cells.
  • Two phases: energy requiring phase & energy generating phase.
  • Important intermediate in glycolysis: glucose-6-phosphate & fructose-1,6-bisphosphate

Anaerobic Glycolysis

  • Re-oxidation of NADH by conversion of pyruvate to lactate via lactate dehydrogenase
  • Occurs in tissues functioning under hypoxic conditions: skeletal muscle, smooth muscle, and erythrocytes.

Poisons that inhibit glycolysis enzymes

  • Iodoacetate: inhibits glyceraldehyde-3-phosphate dehydrogenase.
  • Fluoride: inhibits enolase.
  • Arsenite: interferes with the action of glycolysis enzyme phosphoglycerate kinase

Rapoport-Luebering Cycle

  • Produces 2,3-BPG to regulate oxygen binding in hemoglobin.

Significance of Glycolysis

  • The primary route for glucose metabolism, producing ATP.
  • Also, provides a pathway for fructose and galactose metabolism.
  • Enables tissues to survive anoxic episodes.

Conversion of Pyruvate to Acetyl CoA

  • Pyruvate is converted to acetyl-CoA by oxidative decarboxylation.
  • The reaction is irreversible and requires a multienzyme complex (pyruvate dehydrogenase).
  • PDH requires five coenzymes: thiamine pyrophosphate (TPP), lipoate, coenzyme A, FAD, and NAD+.
  • Produces NADH, essential for ATP production via the electron transport chain.

Citric Acid Cycle (Krebs Cycle or Tricarboxylic Acid (TCA) cycle)

  • A series of reactions in mitochondria transforming acetyl-CoA to CO2 and water.
  • Generates ATP by substrate-level phosphorylation and reducing equivalents that feed the electron transport chain.
  • Moonlighting enzyme aconitase plays critical roles in both enzyme-mediated processes and protein synthesis regulation.

Significance of Citric Acid Cycle

  • Provides energy (ATP).
  • The central oxidative pathway for carbohydrates, lipids, and proteins.
  • Also, provides precursors for various anabolic processes (gluconeogenesis, fatty acid synthesis, heme synthesis, and others).

Gluconeogenesis

  • Synthesis of glucose from non-carbohydrate precursors.
  • Major tissue for gluconeogenesis is the liver; during starvation, the kidney also participates.
  • Relies on some steps from glycolysis in reverse but involves unique reactions to bypass irreversible steps in glycolysis.
  • Precursors include lactate, glycerol, glucogenic amino acids, and propionic acid/intermediates of the TCA cycle.
  • Cori cycle describes the relationship and interchange between the lactate produced by muscle cells to the glucose produced by the liver.

Significance of Gluconeogenesis

  • Maintenance of blood glucose levels in the absence of dietary carbohydrates.
  • Important during periods of fasting or starvation to ensure energy supply for essential organs (brain).
  • Clears metabolic products from other tissues, such as lactate.

Regulation of Gluconeogenesis

  • Four key enzymes, regulated by hormones (glucagon, insulin), and allosteric/ metabolic signals
  • Pyruvate carboxylase, phosphoenolpyruvate carboxykinase, fructose 1,6-bisphosphatase, glucose-6-phosphatase. - Glucagon & epinephrine stimulate gluconeogenesis, insulin inhibits

Glycogen Metabolism

  • Includes glycogenesis (formation of glycogen) and glycogenolysis (degradation of glycogen).
  • Both processes occur in the cytosol, primarily in liver and muscle.

Glycogenesis

  • Pathway forming glycogen from glucose.
  • Requires energy (ATP and UTP).
  • Key enzyme: glycogen synthase

Glycogenolysis

  • Degradation of glycogen to glucose-6-phosphate in muscle and glucose in liver,
  • Involves glycogen phosphorylase and debranching enzymes.
  • Important for rapid blood glucose provision between meals.

Significance of Glycogenolysis and Glycogenesis

  • Maintaining blood glucose levels.
  • Storing excess glucose as glycogen.

Regulation of Glycogenesis and Glycogenolysis

  • Glycogen phosphorylase and glycogen synthase are reciprocally regulated by allosteric and hormonal mechanisms.

Allosteric Regulation of Glycogenesis and Glycogenolysis

  • The enzymes involved have opposing allosteric responses.
  • ATP inhibits glycogen breakdown, AMP stimulates glycolysis
  • Glucose-6-phosphate inhibits glycogen breakdown, glucose-1-phosphate stimulates glycogen synthesis.

Hormonal regulation of glycogen metabolism

  • Glucagon and epinephrine stimulate glycogenolysis and glycogen phosphorylase; insulin inhibits gluconeogenesis by repressing the synthesis of key enzymes

Glycogen Storage Disease

  • Group of inherited disorders from defects in glycogen synthesis or breakdown.
  • Characterized by abnormal glycogen accumulation in specific tissues.
  • Symptoms vary based on affected enzyme & tissue

Pentose Phosphate Pathway

  • Alternative pathway for glucose oxidation, producing NADPH and pentose phosphates.
  • Important for reducing equivalents for biosynthetic processes and antioxidant defense.
  • The enzymes are located in the cytosol of all cells and tissues most enriched in the enzymes include tissues requiring high amounts of NADPH (adrenal gland, testes, ovaries, liver, red blood cells etc)

Significance of Pentose Phosphate Pathway

  • Synthesis of nucleotides, coenzymes, and other important molecules.
  • Produces NADPH (a reducing agent needed for reductive biosynthetic reactions (including the synthesis of fatty acids, sterols, and some amino acids.
  • Protects against oxidative damage by neutralizing reactive oxygen species.

Regulation of Pentose Phosphate Pathway

  • Glucose-6-phosphate dehydrogenase is the rate-limiting enzyme.
  • Its activity is regulated by the concentration of its product, NADPH (competitive inhibition).

Uronic Acid Pathway (Glucuronic Acid Cycle)

  • Pathway in the liver for converting glucose into glucuronic acid and ascorbic acid (except in humans).
  • Pathway is a source for UDP glucuronate.
  • Involved in protein and glycoprotein synthesis and many detoxification processes

Galactose Metabolism and Galactosemia

  • Galactose is derived from the disaccharide lactose.
  • Galactose is readily converted to glucose in the liver and it is incorporated into glycoproteins.
  • Galactosemia is caused by a deficiency in the enzyme galactose-1-phosphate uridyl transferase.
  • Symptoms include elevated galactose blood and urine levels, accumulation of galactose-1-P, galactitol, and lens cataracts.
  • Treatment involves removing galactose from the diet.

Fructose Metabolism

  • Fructose is channelled into the glycolytic pathway.
  • Two pathways for fructose metabolism are the liver fructose-1-phosphate pathway & sorbitol pathway.
  • Essential fructosuria results from a deficiency in fructokinase. Symptoms include fructose excretion in urine.
  • Hereditary fructose intolerance results from a deficiency in the enzyme aldolase B. Symptoms include fructose-1-phosphate buildup, liver and kidney damage and hypoglycemia.

Blood Glucose Level and Its Regulation

  • Blood glucose levels are maintained within a normal range (70-100 mg/dL) through a complex interplay of hormones and metabolic processes.
  • Hormones: Insulin (hypoglycemic) and glucagon (hyperglycemic).
  • Liver is the primary organ controlling blood glucose.
  • Renal mechanism plays a role to control high blood glucose with glycosuria.

Diabetes Mellitus

  • A syndrome of impaired carbohydrate, fat, and protein metabolism.
  • Caused by either lack of insulin secretion or impaired insulin action
  • Two major types are Type I and Type II.

Type I Diabetes Mellitus

  • Characterized by the deficiency of insulin production due to the destruction of the beta cells in the pancreas.
  • Usually begins in childhood/adolescence – acute onset
  • Symptoms include polyuria, polyphagia, polydipsia, ketoacidosis, and weight loss
  • Treatment: requires exogenous insulin administration

Type II Diabetes Mellitus

  • Characterized by insufficient insulin action in tissues that causes increased resistance and deficiency to insulin, resulting in increased blood sugar levels.
  • Usually develops in adulthood – gradual onset
  • Symptoms are gradually developed and usually not accompanied by ketonuria or acidosis
  • Treatment: diet, exercise, weight reduction, possible use of insulin-sensitizing drugs in advanced stages

Gestational Diabetes Mellitus (GDM)

  • High blood sugar levels during pregnancy that resolve after delivery.
  • Women with GDM are at greater risk for developing type II diabetes later in life or pre-eclampsia.

Glucose Tolerance Test (GTT)

  • A test that measures how well the body handles glucose.
  • Used to diagnose diabetes and some related conditions.

Disorders of Glucose Homeostasis

  • Diseases resulting in disturbances of glucose homeostasis are known as metabolic disorders, some of which include inborn errors and/or genetic disorders

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Quiz Team

Related Documents

Carbohydrate Metabolism PDF

More Like This

Untitled Quiz
37 questions

Untitled Quiz

WellReceivedSquirrel7948 avatar
WellReceivedSquirrel7948
Untitled Quiz
55 questions

Untitled Quiz

StatuesquePrimrose avatar
StatuesquePrimrose
Untitled Quiz
50 questions

Untitled Quiz

JoyousSulfur avatar
JoyousSulfur
Untitled Quiz
48 questions

Untitled Quiz

StraightforwardStatueOfLiberty avatar
StraightforwardStatueOfLiberty
Use Quizgecko on...
Browser
Browser