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Questions and Answers

What is the cytoskeleton?

A network of protein filaments extending throughout the cytoplasm of eukaryotic cells.

What are the three main types of protein filaments that make up the cytoskeleton?

  • Actin filaments, Microtubules, Collagen filaments
  • Actin filaments, Microtubules, Intermediate filaments (correct)
  • Actin filaments, Myosin filaments, Intermediate filaments
  • The cytoskeleton is rigid and does not change shape.

    False

    What is the primary role of actin filaments in the cytoskeleton?

    <p>To provide structural support and to be involved in various types of cellular movement.</p> Signup and view all the answers

    What is the diameter of actin filaments?

    <p>7 nanometers</p> Signup and view all the answers

    What are the two main types of actin-binding proteins?

    <p>Actin-bundling proteins and proteins that organize actin networks.</p> Signup and view all the answers

    What are the two main types of actin bundles?

    <p>Parallel bundles and contractile bundles.</p> Signup and view all the answers

    Why are red blood cells useful for studying the cortical cytoskeleton?

    <p>They lack a nucleus and organelles, making it easier to isolate the plasma membrane and associated proteins.</p> Signup and view all the answers

    What is the function of spectrin in the red blood cell cytoskeleton?

    <p>It forms a network with actin filaments that helps maintain the biconcave shape of red blood cells.</p> Signup and view all the answers

    What is the function of ankyrin in the red blood cell cytoskeleton?

    <p>It links the spectrin-actin network to the plasma membrane by binding to spectrin and a transmembrane protein called band 3.</p> Signup and view all the answers

    What is the function of protein 4.1 in the red blood cell cytoskeleton?

    <p>It links spectrin-actin junctions to the transmembrane protein glycophorin.</p> Signup and view all the answers

    What is the function of dystrophin in muscle cells?

    <p>It links actin filaments to transmembrane proteins in the plasma membrane, which in turn link to the extracellular matrix.</p> Signup and view all the answers

    Patients with muscular dystrophy have an increase in dystrophin levels.

    <p>False</p> Signup and view all the answers

    What are stress fibers?

    <p>Large actin bundles that are contractile bundles, cross-linked by alpha-actinin and stabilized by tropomyosin.</p> Signup and view all the answers

    What are focal adhesions?

    <p>Sites of attachment for stress fibers to the extracellular matrix, mediated by transmembrane proteins called integrins.</p> Signup and view all the answers

    What are adherens junctions?

    <p>Cell-cell contacts that form a continuous adhesion belt around each cell, mediated by transmembrane proteins called cadherins.</p> Signup and view all the answers

    What are microvilli?

    <p>Fingerlike extensions of the plasma membrane that are abundant on cells involved in absorption.</p> Signup and view all the answers

    What is the brush border?

    <p>A layer of microvilli on the apical surface of epithelial cells lining the intestine.</p> Signup and view all the answers

    What is the function of myosin I in microvilli?

    <p>It helps attach actin bundles to the plasma membrane in association with the calcium-binding protein calmodulin.</p> Signup and view all the answers

    What are pseudopodia?

    <p>Extensions of moderate width that are responsible for phagocytosis and movement of amoebas.</p> Signup and view all the answers

    What are lamellipodia?

    <p>Broad, sheetlike extensions at the leading edge of fibroblasts.</p> Signup and view all the answers

    What are filopodia?

    <p>Thin projections of the plasma membrane supported by actin bundles.</p> Signup and view all the answers

    What are the three stages of cell movement across a surface?

    <p>Extension of the leading edge, attachment of the leading edge to the substratum, and retraction of the rear of the cell into the cell body.</p> Signup and view all the answers

    What is the role of Rho proteins in cell movement?

    <p>They regulate the formation of cell surface protrusions in response to extracellular stimuli.</p> Signup and view all the answers

    How do Rho proteins activate the formation of branched actin filaments?

    <p>They activate WASP proteins, which then stimulate the Arp2/3 complex.</p> Signup and view all the answers

    What is the role of microtubules in the cytoskeleton?

    <p>To provide structural support and to be involved in various types of cellular movement, including organelle transport and cell division.</p> Signup and view all the answers

    What are the subunits of microtubules?

    <p>Tubulin dimers, which consist of alpha-tubulin and beta-tubulin.</p> Signup and view all the answers

    Microtubules are non-polar structures.

    <p>False</p> Signup and view all the answers

    What is the function of gamma-tubulin in microtubule assembly?

    <p>It helps initiate microtubule assembly at the centrosome.</p> Signup and view all the answers

    What is dynamic instability?

    <p>The alternating cycles of growth and shrinkage that microtubules undergo in response to GTP hydrolysis and the binding of new tubulin subunits.</p> Signup and view all the answers

    The plus end of a microtubule is the end that is typically stabilized.

    <p>False</p> Signup and view all the answers

    What is the function of colchicine and colcemid?

    <p>They inhibit microtubule assembly and are used as experimental tools and in cancer treatments.</p> Signup and view all the answers

    What is the function of vincristine and vinblastine?

    <p>They inhibit microtubule polymerization and are used in cancer chemotherapy.</p> Signup and view all the answers

    What is the function of taxol in microtubule dynamics?

    <p>It stabilizes microtubules, which also blocks cell division.</p> Signup and view all the answers

    What are microtubule-associated proteins (MAPs)?

    <p>Proteins that regulate the dynamic behavior of microtubules.</p> Signup and view all the answers

    What is the function of polymerase MAPs?

    <p>They accelerate microtubule growth by increasing the incorporation of GTP-bound tubulin.</p> Signup and view all the answers

    What is the function of depolymerase MAPs?

    <p>They dissociate GTP-bound tubulin from the plus end of microtubules, leading to microtubule shrinkage or catastrophe.</p> Signup and view all the answers

    What is the function of CLASP proteins?

    <p>They rescue microtubules from catastrophe by stopping disassembly and restarting growth.</p> Signup and view all the answers

    What is the centrosome?

    <p>A microtubule-organizing center in animal cells, from which most microtubules extend outward.</p> Signup and view all the answers

    What is the mitotic spindle?

    <p>A structure formed by microtubules extending outward from duplicated centrosomes during mitosis, which controls chromosome separation and distribution to daughter cells.</p> Signup and view all the answers

    How do plant cells differ from animal cells in terms of microtubule organization?

    <p>Plant cells lack centrosomes, and microtubules instead form an array underlying the plasma membrane, which is involved in plant cell wall synthesis.</p> Signup and view all the answers

    What is the function of the gamma-tubulin ring complex?

    <p>It is a ring-shaped structure in the centrosome that helps initiate microtubule growth, bypassing the rate-limiting nucleation step.</p> Signup and view all the answers

    What are centrioles?

    <p>Cylindrical structures found in most animal cell centrosomes, containing nine triplets of microtubules.</p> Signup and view all the answers

    What are the two families of motor proteins responsible for microtubule-based movements?

    <p>Kinesins and dyneins.</p> Signup and view all the answers

    What is the direction of movement for kinesins along microtubules?

    <p>Towards the plus end.</p> Signup and view all the answers

    What is the direction of movement for dyneins along microtubules?

    <p>Towards the minus end.</p> Signup and view all the answers

    Axonemal dynein was the first identified microtubule motor protein due to its abundance in cilia.

    <p>True</p> Signup and view all the answers

    What is the function of cytoplasmic dynein?

    <p>It moves along microtubules towards the minus end, responsible for transporting cargo towards the minus end of microtubules.</p> Signup and view all the answers

    What is the main function of microtubules in the cell?

    <p>To transport macromolecules, vesicles, and organelles through the cytoplasm.</p> Signup and view all the answers

    Kinesins and dyneins always transport cargo in the same direction.

    <p>False</p> Signup and view all the answers

    Microtubules can help position organelles within the cell.

    <p>True</p> Signup and view all the answers

    What is the role of cytoplasmic dynein in relation to the Golgi apparatus?

    <p>It helps position the Golgi apparatus near the centrosome.</p> Signup and view all the answers

    What are cilia and flagella?

    <p>Microtubule-based projections of the plasma membrane that are responsible for movement in many eukaryotic cells.</p> Signup and view all the answers

    Both cilia and flagella have the same structure, referred to as the axoneme.

    <p>True</p> Signup and view all the answers

    What is the function of nexin in the axoneme?

    <p>It links the A and B tubules of each doublet, helping keep the microtubules in proper arrangement.</p> Signup and view all the answers

    Dynein arms in the axoneme are attached to the B tubule of each doublet.

    <p>False</p> Signup and view all the answers

    What is the function of the basal body?

    <p>It anchors the minus end of microtubules in cilia and flagella, similar in structure to a centriole.</p> Signup and view all the answers

    The movement of cilia and flagella is powered by the sliding of outer microtubule doublets relative to one another.

    <p>True</p> Signup and view all the answers

    How does the mitotic spindle form?

    <p>The centrosome replicates, and the resulting centrosomes migrate to opposite poles of the cell, creating the two poles of the spindle.</p> Signup and view all the answers

    What are the different types of microtubules that make up the mitotic spindle?

    <p>Kinetochore microtubules, chromosomal microtubules, polar microtubules, and astral microtubules.</p> Signup and view all the answers

    What is the function of kinetochore microtubules in mitosis?

    <p>They attach to condensed chromosomes at the centromeres, stabilizing them.</p> Signup and view all the answers

    What is the function of chromosomal microtubules in mitosis?

    <p>They connect to chromosome ends via chromokinesin.</p> Signup and view all the answers

    What is the function of polar microtubules in mitosis?

    <p>They are not attached to chromosomes but are stabilized by overlapping with each other in the center of the cell.</p> Signup and view all the answers

    What is the function of astral microtubules in mitosis?

    <p>They extend outward from the centrosomes with the plus ends anchored in the cell cortex.</p> Signup and view all the answers

    What is the function of chromokinesin?

    <p>It connects chromosomal microtubules to chromosome ends, ensuring proper alignment at the metaphase plate.</p> Signup and view all the answers

    What are the two steps of chromosome movement in anaphase?

    <p>Anaphase A, where chromosomes move towards the poles along kinetochore microtubules, and Anaphase B, where spindle poles separate.</p> Signup and view all the answers

    What is the function of kinesins in Anaphase A?

    <p>They depolymerize and shorten kinetochore microtubules, pulling chromosomes towards the poles of the cell.</p> Signup and view all the answers

    What is the function of plus-end-directed kinesins in Anaphase B?

    <p>They cross-link overlapping interpolar microtubules and move them towards the plus end, pushing spindle poles apart.</p> Signup and view all the answers

    What is the function of cytoplasmic dynein in Anaphase B?

    <p>It is anchored to the cell cortex and moves along astral microtubules towards the minus end, pulling spindle poles apart.</p> Signup and view all the answers

    What are intermediate filaments?

    <p>Filaments that have diameters intermediate between actin filaments and microtubules.</p> Signup and view all the answers

    Intermediate filaments are directly involved in cell movement.

    <p>False</p> Signup and view all the answers

    What are the main types of intermediate filaments?

    <p>Keratins, vimentin, desmin, neurofilament proteins, and nuclear lamins.</p> Signup and view all the answers

    What is the function of keratins?

    <p>They provide structural support in epithelial cells.</p> Signup and view all the answers

    What is the function of vimentin?

    <p>It forms a network that extends from the nucleus towards the cell periphery, providing structural support in various cell types.</p> Signup and view all the answers

    What is the function of desmin?

    <p>It connects the Z discs of individual contractile elements in muscle cells.</p> Signup and view all the answers

    What is the function of neurofilament proteins?

    <p>They provide structural support for long axons in neurons.</p> Signup and view all the answers

    What is the function of nuclear lamins?

    <p>They form a meshwork that underlies the nuclear membrane, providing structural integrity and regulating nuclear shape.</p> Signup and view all the answers

    Intermediate filaments have distinct ends, similar to actin filaments and microtubules.

    <p>False</p> Signup and view all the answers

    Intermediate filaments are more stable than actin filaments and microtubules.

    <p>True</p> Signup and view all the answers

    What is the function of the central rod domain in intermediate filament assembly?

    <p>It plays a central role in filament assembly by forming a coiled coil with another polypeptide.</p> Signup and view all the answers

    What is the function of the head and tail domains in intermediate filaments?

    <p>They determine the specific functions of the intermediate filament protein.</p> Signup and view all the answers

    Intermediate filaments can associate with other cytoskeleton elements.

    <p>True</p> Signup and view all the answers

    What are desmosomes?

    <p>Junctions between adjacent cells that involve the attachment of keratin filaments to dense protein plaques.</p> Signup and view all the answers

    What is the function of desmoplakin?

    <p>It mediates the attachment of keratin filaments to dense protein plaques in desmosomes.</p> Signup and view all the answers

    What are hemidesmosomes?

    <p>Junctions that connect epithelial cells to underlying connective tissue, involving the attachment of keratin filaments to integrins via plakins.</p> Signup and view all the answers

    What is the function of plectin?

    <p>It binds actin filaments and microtubules, forming bridges between them and between intermediate filaments, increasing the mechanical stability of the cell.</p> Signup and view all the answers

    Experimental evidence for the function of intermediate filaments is highly established and has been available for many years.

    <p>False</p> Signup and view all the answers

    What is Epidermolysis bullosa simplex (EBS)?

    <p>A genetic disorder that causes skin blistering from cell lysis after minor trauma, caused by mutations in keratin genes that interfere with the assembly of keratin filaments.</p> Signup and view all the answers

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