Biotechnology Quiz: PCR and DNA Fragment Replication
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Questions and Answers

Which step comes first in the process of creating recombinant DNA?

  • Breaking up genomic DNA using restriction enzymes
  • Isolating desired DNA fragments
  • Inserting the plasmid with the desired fragment into a host cell
  • Cleaving a plasmid using restriction enzymes (correct)
  • What is the term used to describe when a cell takes in DNA from outside the cell?

  • DNA sequencing
  • Genetic engineering
  • Cloning
  • Transformation (correct)
  • Which of the following names small, circular DNA molecules found in bacterial cells?

  • Plasmids (correct)
  • Restriction enzymes
  • Genomes
  • Ligases
  • In the process of creating recombinant DNA, what is the final step?

    <p>Inserting the plasmid with the desired fragment into a host cell</p> Signup and view all the answers

    Why do some plasmids contain a gene for resistance to an antibiotic?

    <p>To protect the bacteria from the antibiotic</p> Signup and view all the answers

    What is the purpose of using DNA ligase in creating recombinant DNA?

    <p>To insert desired DNA fragments into a plasmid</p> Signup and view all the answers

    What did scientists accomplish by inserting a gene from an ocean jellyfish into a mosquito?

    <p>The genes of the mosquito were manipulated using genetics.</p> Signup and view all the answers

    What was the main goal of the Human Genome Project completed in 2003?

    <p>Identifying and mapping all human genes.</p> Signup and view all the answers

    Which process is involved in genetic engineering?

    <p>Manipulating an organism's genetic material.</p> Signup and view all the answers

    In genetic engineering, what is the purpose of using DNA fingerprinting?

    <p>Identifying unique genetic markers in an organism.</p> Signup and view all the answers

    Which of the following is a potential benefit of genetic engineering?

    <p>Reduced reliance on chemical pesticides.</p> Signup and view all the answers

    Why is it important to categorize descriptions of genetic engineering as benefits or risks?

    <p>To understand the impact and consequences of genetic engineering.</p> Signup and view all the answers

    Which statement about the human genome is false?

    <p>The human genome contains nucleotide sequences that all code for proteins.</p> Signup and view all the answers

    What is the scientific field devoted to creating and maintaining databases about gene sequences of organisms?

    <p>Bioinformatics</p> Signup and view all the answers

    What technique looks at separated DNA fragments to identify unique patterns that are unique to every individual?

    <p>DNA typing</p> Signup and view all the answers

    How many genes are approximately present in the human genome?

    <p>30,000 genes</p> Signup and view all the answers

    What is the main focus of proteomics?

    <p>Studying proteins and their functions</p> Signup and view all the answers

    How might the study of the human genome lead to better health?

    <p>By creating personalized medicine based on genetic information</p> Signup and view all the answers

    What is the main result of a polymerase chain reaction?

    <p>Millions of replicated DNA fragments</p> Signup and view all the answers

    Which process uses electric pulsation or heat to create openings in the plasma membrane of bacterial cells?

    <p>Transformation</p> Signup and view all the answers

    Based on the given sequences, which restriction enzyme produces a blunt end?

    <p>EagI</p> Signup and view all the answers

    During cell replication, what happens to recombinant plasmid DNA?

    <p>It is copied and present in new cells</p> Signup and view all the answers

    What is the primary purpose of inserting exogenous DNA into the DNA of another organism?

    <p>To study the DNA sequences further</p> Signup and view all the answers

    Which technique is commonly used to identify and study the DNA sequence of cloned recombinant DNA molecules?

    <p>Gel electrophoresis</p> Signup and view all the answers

    What is the term for silicon chips or microscope slides with DNA fragments that allow many genes in a genome to be studied simultaneously?

    <p>Recombinant DNA</p> Signup and view all the answers

    Which technique can make copies of specific regions of a DNA fragment?

    <p>Polymerase chain reaction</p> Signup and view all the answers

    What technology is used to manipulate an organism's DNA by inserting the DNA of another organism?

    <p>Genetic engineering</p> Signup and view all the answers

    Which technique is used to correct mutated disease-causing genes?

    <p>Gene therapy</p> Signup and view all the answers

    What is the term for a newly generated DNA fragment containing exogenous DNA?

    <p>Recombinant DNA</p> Signup and view all the answers

    (61) , the study of an organism's genome, has paved the way for , which is the study of the structure and function of proteins, by cataloging the genes in an organism that code for various proteins.

    <p>DNA microarray</p> Signup and view all the answers

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