Biotechnology in Food Industry
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Questions and Answers

What is the primary function of the impeller in a fermenter?

  • To trap contaminants
  • To introduce sterile oxygen
  • To prevent cells from settling (correct)
  • To regulate flow rates
  • Which type of fermentation allows for continuous addition of fresh media?

  • Static reactors
  • Continuous reactors (correct)
  • Cyclic reactors
  • Batch reactors
  • What type of fermentation condition is applicable only to small fermenters?

  • Aerobic and Anaerobic
  • Requires high pH control
  • Aerobic only (correct)
  • Requires continuous monitoring
  • Which component is used to measure the flow rate of air or liquid in a fermenter?

    <p>Rota meter (B)</p> Signup and view all the answers

    What is the role of baffles in a fermenter?

    <p>To disrupt vortices and improve mixing (D)</p> Signup and view all the answers

    Which of the following best defines a facultative organism?

    <p>An organism that can grow under certain conditions but does not require them. (B)</p> Signup and view all the answers

    What are the three cardinal thermal points defining temperature growth range?

    <p>Minimum, optimum, and maximum growth temperature. (D)</p> Signup and view all the answers

    What influences the maximum growth temperature for microbes?

    <p>Inactivation of enzymes at elevated temperatures. (B)</p> Signup and view all the answers

    What aspect makes oxygen poisonous to obligate anaerobes?

    <p>Oxygen forms toxic reactive oxidizing agents that can damage cells. (B)</p> Signup and view all the answers

    Which factor is NOT directly a physical requirement for microbial growth?

    <p>Nitrogen (A)</p> Signup and view all the answers

    What characterizes a fed batch reactor in the fermentation process?

    <p>Fresh media is added continuously or periodically. (D)</p> Signup and view all the answers

    Which term describes organisms that can survive extreme conditions but do not grow?

    <p>Thermotolerant (D)</p> Signup and view all the answers

    What do the terms 'phile' and 'tolerant' specifically refer to regarding microbial growth?

    <p>'-phile' describes conditions where growth is possible, while 'tolerant' refers to survival in unfavorable conditions. (D)</p> Signup and view all the answers

    In a fermentation triangle, which components represent its three sides?

    <p>Bio-reactor, Biocatalyst, and Raw Materials (B)</p> Signup and view all the answers

    Which statement is true regarding the growth of thermophilic and thermotolerant bacteria?

    <p>Thermophilic bacteria require elevated temperatures for growth, while thermotolerant bacteria can survive those conditions but grow at lower temperatures. (A)</p> Signup and view all the answers

    Which of the following types of microorganisms can grow in the presence of oxygen but can also survive in its absence?

    <p>Facultative anaerobes (D)</p> Signup and view all the answers

    What term describes microorganisms that can only grow at low concentrations of oxygen, specifically between 2-10%?

    <p>Microaerophiles (A)</p> Signup and view all the answers

    Which type of medium contains a known exact chemical composition?

    <p>Chemically defined media (C)</p> Signup and view all the answers

    Which of the following organisms is classified as an obligate aerobe?

    <p>Mycobacterium tuberculosis (A)</p> Signup and view all the answers

    What are examples of macronutrients required by microorganisms in large amounts?

    <p>C, N, and S (C)</p> Signup and view all the answers

    Flashcards

    Batch Fermenter

    A simple type of fermenter where the fermentation process fills, runs, empties, and then repeats the process.

    Continuous Fermenter

    A fermenter where fresh media is consistently added, and culture fluid is continuously removed.

    Fermenter Impeller

    A stirrer in a fermenter that prevents cell settling and distributes oxygen.

    Fermenter Sparger

    Introduces sterile oxygen to the aerobic fermentation media.

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    Fermenter Size/Growth Rate Relation

    Large fermenters can handle more growth in a shorter time.

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    Obligate vs. Facultative

    Obligate organisms require a specific condition for growth, while facultative organisms can grow under that condition but don't require it.

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    Facultative Thermophile

    A microbe that can grow in both elevated and lower temperatures, but doesn't require high temperatures like an obligate thermophile.

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    Cardinal Thermal Points

    Three key temperatures that define the growth range of a microbial species: minimum, optimum, and maximum.

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    Optimum Growth Temperature

    The temperature at which a microbial species grows best.

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    Oxygen Requirements: Obligate Anaerobe

    A microbe that cannot grow in the presence of oxygen and is killed by it.

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    Fed Batch Reactor

    A reactor where fresh media is continuously or periodically added, making it the most common type in industry.

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    Fermentation Triangle

    A representation with three sides: Bioreactor, Biocatalyst, and Purification, where the material surface represents the integration of all factors for a successful fermentation process.

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    Microbe Growth & Phile/Tolerance

    Growth refers to biomass acquisition leading to cell division. While organisms can survive in certain conditions, 'phile' indicates growth while 'tolerant' indicates survival but not growth.

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    Thermophile vs. Thermotolerant

    A thermophile grows optimally at high temperatures, while a thermotolerant organism can survive but grows best at lower temperatures.

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    Obligate Aerobes

    Organisms that require oxygen for survival and growth. They use oxygen as the final electron acceptor during aerobic respiration.

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    Facultative Anaerobes

    Organisms that can thrive in both the presence and absence of oxygen. They prefer oxygen for aerobic respiration but can switch to anaerobic respiration or fermentation when oxygen is unavailable.

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    Aerotolerant Anaerobes

    Organisms that don't use oxygen for growth, but can tolerate its presence. They have enzymes to detoxify harmful oxygen byproducts.

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    Microaerophiles

    Organisms that require low oxygen levels for growth. High oxygen concentrations can be toxic.

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    What are growth factors?

    Essential organic compounds that a microbe can't synthesize on its own. Examples include amino acids for protein synthesis and purines/pyrimidines for nucleic acid synthesis.

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    Study Notes

    Biotechnological Applications in Food Industry

    • Dr. Eman Owis, lecturer of microbial biotechnology at Mansoura University, Germany, presented on biotechnological applications in the food industry.
    • Topics discussed included fermentation biotechnology, genetically modified food, enzymes in the food industry, and nanotechnology in food.

    Food Biotechnology

    • Biotechnology uses microorganisms (bacteria, yeast, molds) to create food products.
    • Factors influencing microbial activity include importance of bacteria and yeasts in the food industry, fermentation types, and molecular biology techniques.
    • Food biotechnology safety and regulations are discussed.
    • Different techniques associated with food biotechnology are also discussed.

    Fermentation

    • Fermentation is a process where organisms convert organic matter into simpler substances.
    • Fermentation processes can either be aerobic or anaerobic and rely on microorganisms.
    • The process can be divided into batch reactors and continuous reactors.
    • The fresh media is often added and the resulting substances are removed from the bioreactor.

    Fermentation Triangle

    • Microorganisms, and purification in the process of fermentation are discussed within the context of the fermentation triangle.
    • The integration of raw materials (media) completes the processes.

    Factors Influencing Growth

    • "Growth" refers to biomass accumulation, cell division, and reproduction.
    • Microbes can survive in conditions where they can't grow.
    • "-phile" often describes growth conditions.
    • Tolerance conditions mean organisms survive without necessarily growing.
    • Specific examples are "thermophilic bacterium" which grows at elevated temperatures versus "thermotolerant bacterium" that survives elevated temperature but grows at lower temperatures.

    Obligate vs. Facultative

    • Obligate means conditions are required for growth.
    • Facultative means the organism can grow under the condition, but doesn't require it.
    • Example: an obligate thermophile requires elevated temperatures for growth, while a facultative thermophile grows at either elevated or lower temperatures.

    Growth Requirements

    • Physical requirements include temperature, pH, light, radiation, and water.
    • Chemical requirements include carbon, nitrogen, sulfur, phosphorous, trace elements, oxygen, and organic growth factors.

    Temperature Optima

    • Microbial cells adapt to their environment's ambient temperature.
    • Growth requires three key temperatures (minimum, optimum, and maximum).
    • Optimum temperature is generally near the highest part of the growth range.
    • Death results from enzyme inactivation at higher temperatures.
    • Mesophiles are the most common group of organisms.

    Oxygen Requirements

    • Aerobic respiration requires oxygen.
    • Anaerobic respiration does not require oxygen.
    • Obligate aerobes need oxygen, while obligate anaerobes are killed by oxygen.
    • Facultative anaerobes can grow with or without oxygen.
    • Aerotolerant bacteria are unaffected by oxygen, while.
    • Microaerophiles require low oxygen levels.

    Microbial Nutrition (Media)

    • Microbial growth needs liquid or solid preparations.
    • Media can be chemically defined (known composition) or complex (unknown composition, plant/animal extracts).
    • Required components like water, macro elements (C, N, O, H, S, P, K), micro-elements (Mn, Zn, Co) are required and often supplied in media or water.
    • Growth factors (amino acids, purines & pyrimidines, vitamins) are also essential for growth.

    Growth in Batch Culture

    • Batch culture (closed system) has four phases: lag, logarithmic, stationary, and death.
    • Lag phase: adjustment period with no visible growth but active metabolism.
    • Log phase: rapid, exponential cell growth.
    • Stationary phase: equilibrium between growth and death.
    • Death phase: death rates exceed growth rates.

    Bacterial Growth

    • Bacterial division follows a logarithmic progression.
    • Generation time is the time a population doubles.
    • This time varies based on the microorganism.
    • Example microorganisms like Escherichia coli and Mycobacterium tuberculosis have different generation times.

    Growth in Filamentous Organisms

    • Filamentous organisms grow on both liquid and solid media.
    • Growth stages include lag, rapid, and retarded growth, which are followed by stationary phase and death.

    Typical Industrial Fermentation Process

    • The process involves upstream processing (raw materials), fermentation, and downstream processing (separation, purification).
    • Downstream processing includes methods like precipitation, deionization, chromatography, evaporation, filtration, crystallization, and drying.

    Outputs of Lecture

    • Principles of fermentation, fermenters/bioreactors, and downstream processing.

    Differences between fermenter and bioreactor

    • Fermenters are typically used for bacteria and fungi.
    • Bioreactors are used for mammalian cells.
    • Key differences include size, growth rate, pH control methods, and foam control.

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    Description

    Explore the applications of biotechnology in the food industry, including fermentation, genetically modified foods, and the use of enzymes. Learn about the regulatory aspects and the importance of microbial activity in food production. This quiz provides insights into the advanced techniques that are shaping modern food technology.

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