6 Questions
Nutrients, cells, reagents -----------------→PRODUCT
are provided at the beginning of the cultivation and products are removed at the end
Shorter productive time. Productivity is lo
w
Shake Flask Seed Fermenter Production Fermenter
Stages of Batch Fermentation
The bioreactor is first charged with medium, inoculated with cells, and the cells achieve the required density or optimum product concentrations. The bioreactor contents are discharged, and the bioreactor is prepared for a fresh charge of medium
Batch Culture
All nutrients are provided at the beginning of the cultivation and products are removed at the end
Advantages
The Stages of ______ Shake Flask Seed Fermenter Production Fermenter
Batch Fermentation
Study Notes
Batch Culture
- A type of culture operation in a closed culture system, where an initial limited amount of nutrient is provided.
- The inoculated culture or inoculum cells undergo several phases in the growth curve as they grow.
Phases of Batch Fermentation
- Shake Flask: Initial stage of fermentation.
- Seed Fermenter: Stage where cells achieve the required density or optimum product concentrations.
- Production Fermenter: Final stage where products are removed.
Characteristics of Batch Fermentation
- All nutrients are provided at the beginning of the cultivation and products are removed at the end.
- Nutrients, cells, and reagents are converted into products.
Advantages of Batch Fermentation
- Short duration of the process.
- Less chance of contamination since nutrients are added at the beginning.
- Easy separation of batch material for traceability.
- Easier to manage the process.
Disadvantages of Batch Fermentation
- Product is mixed with nutrients, reagents, cell debris, and toxins.
- Shorter productive time.
- Productivity is low.
Test your knowledge of batch culture in biotechnology with this quiz. Explore the phases of the growth curve and the operation of a closed culture system, including nutrient limitations and cell density achievement. Ideal for students and professionals in bioprocessing and bioreactor operation.
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