16 Questions
What is the main purpose of biotechnology?
To use living organisms for human benefit
Which historical examples of biotechnology are mentioned in the text?
Use of antibiotics and selective breeding
What is the process of heating and cooling called in Polymerase Chain Reaction (PCR)?
Thermocycling
What is the main role of DNA primers in the Annealing stage of PCR?
Helping to attach to the template DNA
What is used to check the quantity and size of the DNA fragments produced after PCR?
Gel electrophoresis
What is the main purpose of the Taq polymerase enzyme in PCR?
Making new strands of DNA
Which enzyme isolated from Thermus aquaticus is commonly used in molecular cloning?
DNA polymerase
What is the function of a cloning vector in gene cloning?
Allows for DNA replication
Which feature of a good DNA cloning vector allows for recognition sites for several restriction enzymes?
Multiple cloning site (MCS)
What is the main limitation of bacterial plasmid vectors in DNA cloning?
Copy number problems
Which type of vector is NOT ideal for protein expression in gene cloning?
Cosmid (circular)
What is the maximum insert size for bacterial plasmid vectors?
~6-12 kb
What type of vector has packaging limits for DNA insert size?
Bacteriophage vectors (linear)
Which vector type allows for cloning large DNA fragments and genomic libraries?
~35-45 kb BAC
"Yeast artificial chromosomes (YACs)" are used for cloning into which organism?
Saccharomyces cerevisiae
"Taq DNA polymerase" is isolated from which species known for thriving in hot springs?
Thermus aquaticus
Explore the field of biotechnology and synthetic biology with this PowerPoint® lecture presentation. Learn about the historical examples and the definition of biotechnology, its impact on everyday lives, and its rapidly changing nature.
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