Biotechnology and Synthetic Biology: Chapter 12 Lecture Presentation
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Questions and Answers

What is the main purpose of biotechnology?

  • To create synthetic life forms
  • To study living organisms
  • To use living organisms for human benefit (correct)
  • To manipulate DNA
  • Which historical examples of biotechnology are mentioned in the text?

  • Use of antibiotics and selective breeding (correct)
  • Artificial intelligence and nanotechnology
  • Stem cell research and gene therapy
  • Cloning and genetic modification
  • What is the process of heating and cooling called in Polymerase Chain Reaction (PCR)?

  • Temperature cycling
  • Thermocycling (correct)
  • Thermal regulating
  • Heat cycling
  • What is the main role of DNA primers in the Annealing stage of PCR?

    <p>Helping to attach to the template DNA</p> Signup and view all the answers

    What is used to check the quantity and size of the DNA fragments produced after PCR?

    <p>Gel electrophoresis</p> Signup and view all the answers

    What is the main purpose of the Taq polymerase enzyme in PCR?

    <p>Making new strands of DNA</p> Signup and view all the answers

    Which enzyme isolated from Thermus aquaticus is commonly used in molecular cloning?

    <p>DNA polymerase</p> Signup and view all the answers

    What is the function of a cloning vector in gene cloning?

    <p>Allows for DNA replication</p> Signup and view all the answers

    Which feature of a good DNA cloning vector allows for recognition sites for several restriction enzymes?

    <p>Multiple cloning site (MCS)</p> Signup and view all the answers

    What is the main limitation of bacterial plasmid vectors in DNA cloning?

    <p>Copy number problems</p> Signup and view all the answers

    Which type of vector is NOT ideal for protein expression in gene cloning?

    <p>Cosmid (circular)</p> Signup and view all the answers

    What is the maximum insert size for bacterial plasmid vectors?

    <p>~6-12 kb</p> Signup and view all the answers

    What type of vector has packaging limits for DNA insert size?

    <p>Bacteriophage vectors (linear)</p> Signup and view all the answers

    Which vector type allows for cloning large DNA fragments and genomic libraries?

    <p>~35-45 kb BAC</p> Signup and view all the answers

    "Yeast artificial chromosomes (YACs)" are used for cloning into which organism?

    <p><em>Saccharomyces cerevisiae</em></p> Signup and view all the answers

    "Taq DNA polymerase" is isolated from which species known for thriving in hot springs?

    <p><em>Thermus aquaticus</em></p> Signup and view all the answers

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