Biotechnology and Synthetic Biology: Chapter 12 Lecture Presentation
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Questions and Answers

What is the main purpose of biotechnology?

  • To create synthetic life forms
  • To study living organisms
  • To use living organisms for human benefit (correct)
  • To manipulate DNA

Which historical examples of biotechnology are mentioned in the text?

  • Use of antibiotics and selective breeding (correct)
  • Artificial intelligence and nanotechnology
  • Stem cell research and gene therapy
  • Cloning and genetic modification

What is the process of heating and cooling called in Polymerase Chain Reaction (PCR)?

  • Temperature cycling
  • Thermocycling (correct)
  • Thermal regulating
  • Heat cycling

What is the main role of DNA primers in the Annealing stage of PCR?

<p>Helping to attach to the template DNA (D)</p> Signup and view all the answers

What is used to check the quantity and size of the DNA fragments produced after PCR?

<p>Gel electrophoresis (B)</p> Signup and view all the answers

What is the main purpose of the Taq polymerase enzyme in PCR?

<p>Making new strands of DNA (C)</p> Signup and view all the answers

Which enzyme isolated from Thermus aquaticus is commonly used in molecular cloning?

<p>DNA polymerase (A)</p> Signup and view all the answers

What is the function of a cloning vector in gene cloning?

<p>Allows for DNA replication (B)</p> Signup and view all the answers

Which feature of a good DNA cloning vector allows for recognition sites for several restriction enzymes?

<p>Multiple cloning site (MCS) (D)</p> Signup and view all the answers

What is the main limitation of bacterial plasmid vectors in DNA cloning?

<p>Copy number problems (B)</p> Signup and view all the answers

Which type of vector is NOT ideal for protein expression in gene cloning?

<p>Cosmid (circular) (B)</p> Signup and view all the answers

What is the maximum insert size for bacterial plasmid vectors?

<p>~6-12 kb (D)</p> Signup and view all the answers

What type of vector has packaging limits for DNA insert size?

<p>Bacteriophage vectors (linear) (C)</p> Signup and view all the answers

Which vector type allows for cloning large DNA fragments and genomic libraries?

<p>~35-45 kb BAC (D)</p> Signup and view all the answers

"Yeast artificial chromosomes (YACs)" are used for cloning into which organism?

<p><em>Saccharomyces cerevisiae</em> (B)</p> Signup and view all the answers

"Taq DNA polymerase" is isolated from which species known for thriving in hot springs?

<p><em>Thermus aquaticus</em> (C)</p> Signup and view all the answers

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