Biosensor Assays and Monoclonal Antibodies Quiz

In a sandwich assay format, the signal is detected on the sensor and the variable region of the antibody binds to the antigen. In a competitive assay format, the signal is detected off the sensor and there is competition between the analyte and a labeled analog (A-gen) for binding to the antibody.

If the analyte is S, and the enzyme makes 105 molecules of labeled product/sec, then after 100 seconds, the number of molecules of labeled product would be $10 \times 10^7 = 10^8$.

If there are 100 Enz molecules coupled to the AB, and the enzyme makes 105 molecules of labeled product/sec, then after 100 seconds, the number of molecules of labeled product would be $10 \times 10^9 = 10^{10}$.

<p>The signal amplification process involves the addition of substrate, which leads to the enzyme producing multiple molecules of labeled product for detection.</p> Signup and view all the answers

<p>The text discusses electrochemistry and electrochemical biosensors, as well as semiconductors and nanotechnology.</p> Signup and view all the answers

<p>Oxidation is the loss of electrons, while reduction is the gain of electrons in the context of electron transfer.</p> Signup and view all the answers

<p>Substances that have the ability to oxidize other substances (i.e. grab their electrons) are called oxidants, while substances that have the ability to reduce other substances (i.e. give electrons away) are called reductants. They relate to oxidation and reduction by either accepting or donating electrons in redox reactions.</p> Signup and view all the answers

<p>LEO stands for 'lose electrons oxidation' and GER stands for 'gain electrons reduction.' This mnemonic helps to remember that in redox reactions, the substance that loses electrons undergoes oxidation, while the substance that gains electrons undergoes reduction.</p> Signup and view all the answers