Biosafety Training and Regulations Quiz

Questions and Answers

What is a critical component of biosafety training at CRCHUSJ?

Introduction to data analysis software

Procedure orientations specific to the project (correct)

General public health reporting

Training on laboratory marketing strategies

Which vaccination is specifically mentioned as important for individuals working with infectious materials?

Measles

Hepatitis B

Tetanus (correct)

Influenza

Which document should be completed by both the student and the supervisor during training?

The incident report form

The form read and understood (correct)

The medical surveillance registration form

The training document signature form

What general safety measure must be implemented before entering a laboratory?

Informing employees of possible biological risks Signup and view all the answers

Which group offers training on biosafety cabinets and spill procedures at CRCHUSJ?

The Advanced BioSafety team Signup and view all the answers

What should be done with laboratory doors to maintain safety?

Keep them closed Signup and view all the answers

What documents should trainees ensure to include in their training document?

Name and signature of the research supervisor Signup and view all the answers

Why is medical surveillance important for individuals working in laboratories?

To monitor for potential laboratory-acquired infections Signup and view all the answers

What is the primary reason for restricting access to a CL2 laboratory?

To prevent unauthorized use Signup and view all the answers

Which of the following practices are essential for minimizing environmental contamination in CL2 laboratories?

Regular hand washing and decontamination Signup and view all the answers

What type of procedures should be performed inside an ESB certified to NSF 49?

Aerosol-generating procedures Signup and view all the answers

When should lab coats and gloves be removed?

If the employee leaves the laboratory for any reason Signup and view all the answers

What is one of the main containment devices required in CL2 laboratories?

Centrifuges fitted with sealed rotors Signup and view all the answers

Which action is NOT recommended after handling infectious material in a CL2 laboratory?

Relying on gloves for hand safety Signup and view all the answers

Which document outlines the procedures to be adopted in CL2 laboratories?

PNF ‐ CELL ‐ 102.00_Salles_cultures_communes_NC2_CRCHUSJ Signup and view all the answers

What is a critical component of the personal protective equipment required in CL2 laboratories?

Protective glasses Signup and view all the answers

What must an employee complete before performing any high-risk task in a laboratory?

Biosafety guidelines relevant to their facilities Signup and view all the answers

Which of the following is NOT a responsibility of the ABS regarding new employees?

Monitoring the employee's performance post-training Signup and view all the answers

What is one of the outcomes of successful biosafety training for participants?

Demonstrating a good understanding of the risk assessment procedure Signup and view all the answers

What aspect of biohazard laboratories is related to the concept of containment levels?

The classification of microorganisms based on risk Signup and view all the answers

Access to training materials to meet biosafety requirements is stored in which database?

CRCHUSJ database Signup and view all the answers

What should be documented following a participant's biosafety training?

The specific training courses and orientations taken Signup and view all the answers

What should laboratory staff be able to describe after completing biosafety training concerning biohazardous materials?

The procedures to follow in case of accidental exposure Signup and view all the answers

What is emphasized as a necessary factor in the training of new employees working with biohazardous materials?

The appropriateness of the training to specific biological processes Signup and view all the answers

Study Notes

Training and Regulations

Training for new staff is established during the hiring process, aligning with Human Pathogens and Toxins Regulations.
Employees must complete biosafety guidelines relevant to their facility and laboratory space before undertaking high-risk tasks.
Documentation of all biosafety training and orientations is crucial and maintained in the CRCHUSJ database.

Mandatory Biosecurity Training

Mandatory training applies to principal investigators, supervisors, research staff, monitors, and students working with microorganisms, cell cultures, blood, and human body fluids.
The Animal Biosafety Subcommittee (ABS) oversees appropriate training linked to biological processes in the lab.
Training must be completed before commencing work and is documented by ABS.

Goals and Content of Biosafety Training

Participants should understand risk assessment procedures related to working with microorganisms and cell lines.
Knowledge of containment levels applicable to biohazard labs is necessary.
Trainees must learn about biological safety cabinets (BSEs) and spill response procedures.
Participants need to understand the risks associated with human blood and body fluids and how to take necessary precautions when handling these materials.

Essential Training Documentation

Training records should include:
- Names and signatures of the research supervisor and trainee.
- Instructor’s name.
- Date training was completed.
CRCHUSJ biosafety training includes:
- Theoretical information from Public Health Agency of Canada (PHAC) courses.
- Orientation on fundamental biosafety, BSEs, autoclaves, and spill procedures.
- Project-specific training by the principal investigator.

Medical Surveillance and Vaccination

Workers handling infectious materials risk laboratory-acquired infections (LAIs).
Students are advised to keep immunizations up to date, including tetanus every ten years.
Health questions or conditions requiring surveillance should be addressed through the CHU Sainte-Justine health office.

General Safety Measures and Procedures

Laboratory personnel must be aware of biological risks before entering the work area.
Laboratory doors should remain closed to prevent unauthorized access.
Biological agents requiring CL2 installations typically do not transmit via air; precautions are necessary to reduce aerosol generation.

CL2 Laboratory Practices

Access to CL2 laboratories is restricted to trained and authorized personnel.
Compliance with personal protective equipment guidelines is mandatory.
Work surfaces must be decontaminated at the start and end of each day, as well as after spills.
All infectious waste must be decontaminated before disposal.
Hand hygiene is essential; hands should be washed after handling infectious materials.
Aerosol-generating procedures must occur within NSF 49 certified BSEs.
Lab coats are to be kept inside the lab and marked accordingly; gloves should be removed when leaving the laboratory.

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Description

Test your knowledge on the essential biosafety training required for laboratory personnel. Understand the guidelines and regulations that ensure safe practices in handling human pathogens and toxins. This quiz will help you grasp the importance of adequate training and documentation in a laboratory setting.