Bioprocess Engineering in Biotechnology

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30 Questions

What is the basic unit of life?

Cell

What are the collective processes involving nutrient consumption, cell growth, and product synthesis called?

Metabolic processes

Which type of cells do not contain a membrane-enclosed nucleus?

Procaryotic cells

What term is used for the industrial-scale production of useful products by harnessing metabolic processes?

Biochemical engineering

What distinguishes eukaryotes from prokaryotes in terms of cellular structures?

Presence of membrane-enclosed nucleus

Which cells are traditionally the main focus of biochemical engineering?

Microbial cells

What is the shape of bacteria classified as 'cocci'?

Spherical or ovoid shaped

Which category of bacteria can survive temperatures between 7 to 45 oC?

Mesophilic

What is the characteristic of microaerophilic bacteria?

Survives in minute amounts of oxygen

Where can peritrichous flagella be located on a bacterial cell?

Anywhere in the cell

What are spores in bacteria known for?

Withstanding extreme conditions

What differentiates pili/fimbrae from flagella in bacteria?

Length

Which major class of enzymes is responsible for Functional group transfer?

Transferases

What is the main requirement for successful application of biotechnology?

Integration of scientific disciplines and technologies

What type of reaction do Oxidoreductases catalyze?

Oxidation-reduction

Which area of biotechnology is highlighted as important for its commercialization?

Bioprocess Engineering

Which enzyme class is involved in the removal of a group, leaving a double bond?

Hydrolases

What is the main function of Isomerases?

Isomerization

What are some roles of a biochemical engineer in the commercial realization of biotechnology?

Scale-up, design, operation and control of bioreactors

What tools are commonly used in biochemical engineering?

Quantitative models and approaches

In enzyme classification, which class is mainly involved in the addition of a group to a double bond to form a new double bond or ring structure?

Ligases (synthetases)

What makes biological systems amenable to engineering analysis according to the text?

They obey the laws of chemistry and physics

If an enzyme is involved in the joining of two molecules coupled with the cleavage of a pyrophosphate bond, to which major class does it belong?

Ligases (synthetases)

In which aspect of bioprocessing is substantial engineering input essential?

Design and optimal operation of bioreactors

What does Km represent in the Briggs and Haldane approach?

The product-releasing step

In what scenario will Km and K20 be the same in the Briggs and Haldane approach?

When the product-releasing step is slower

What occurs in uncompetitive inhibition?

The inhibitor does not bind to the enzyme-substrate complex

What effect does substrate inhibition have on enzyme activity?

Decreases activity at low substrate concentrations

What is the relationship between vmax app and Km, app in enzyme kinetics?

vmax app and Km, app are inversely related

What characterizes an enzyme-substrate complex in uncompetitive inhibition?

Formation of a stable complex

Explore the importance of bioprocess engineering in the successful application of biotechnology, integrating microbiology, biochemistry, genetics, and engineering know-how. Learn about the key areas vital for the commercialization of biotechnological processes.

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