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Questions and Answers
What is the primary focus of Biomedical technique?
What is the primary focus of Biomedical technique?
- Utilization of engineering principles in everyday life
- Application of medical principles only
- Application of engineering principles to the medical field (correct)
- Development of new technologies for entertainment
What is the primary function of a photocell?
What is the primary function of a photocell?
- To convert light energy into electric energy (correct)
- To measure current in a circuit
- To convert electric energy into light energy
- To amplify electrical signals
How is the potential difference generated in a photocell typically measured?
How is the potential difference generated in a photocell typically measured?
- With a voltmeter
- With an ammeter
- With an oscilloscope
- With a galvanometer (correct)
What characterizes a blank solution in an assay?
What characterizes a blank solution in an assay?
Which component is essential for measuring the current generated in a photocell?
Which component is essential for measuring the current generated in a photocell?
In a laboratory setup involving a photocell, which component is likely used to hold the optical sample?
In a laboratory setup involving a photocell, which component is likely used to hold the optical sample?
What is the primary principle behind atomic absorption spectroscopy (AAS)?
What is the primary principle behind atomic absorption spectroscopy (AAS)?
What specific aspect does atomic absorption spectroscopy rely on?
What specific aspect does atomic absorption spectroscopy rely on?
Which of the following statements accurately describes atomic absorption spectroscopy?
Which of the following statements accurately describes atomic absorption spectroscopy?
In atomic absorption spectroscopy, what happens to free metallic ions?
In atomic absorption spectroscopy, what happens to free metallic ions?
Why is atomic absorption spectroscopy particularly useful?
Why is atomic absorption spectroscopy particularly useful?
Who first observed the phenomenon related to dark lines in the solar spectrum?
Who first observed the phenomenon related to dark lines in the solar spectrum?
What did Fraunhofer study to make his observations about dark lines?
What did Fraunhofer study to make his observations about dark lines?
Which scientific principle did Alan Walsh utilize in his work with atomic absorption spectroscopy?
Which scientific principle did Alan Walsh utilize in his work with atomic absorption spectroscopy?
What is the main application of atomic absorption spectroscopy as utilized by Alan Walsh?
What is the main application of atomic absorption spectroscopy as utilized by Alan Walsh?
What is the primary purpose of a filter in a monochromator?
What is the primary purpose of a filter in a monochromator?
Which materials can be used for a filter in a monochromator?
Which materials can be used for a filter in a monochromator?
How does a monochromator select a wavelength of light?
How does a monochromator select a wavelength of light?
What characteristic defines a monochromatic light source?
What characteristic defines a monochromatic light source?
Why is it important to select a single wavelength of light in experiments?
Why is it important to select a single wavelength of light in experiments?
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Study Notes
Biomedical Technique
- Application of engineering principles and techniques to the medical field.
Components of a Photometer
- Photocell (detector): Converts light energy into electric energy.
- Galvanometer (amplifier/readout): Measures the potential difference (current) generated in the photocell.
###Â Components of a Spectrophotometer
- Source of light: Provides a beam of light.
- Entrance slit: Controls the width of the light beam.
- Gratings (monochromator): Selects a specific wavelength of light.
- Detector: Measures the intensity of the light beam.
- Cuvette holder: Holds the sample solution.
Blank Solution
- Contains all the reagents of the assay, except the compound (sample) being measured.
Atomic Absorption Spectroscopy
- This procedure depends on the absorption of light by free metallic ions.
- Based upon the absorption and emission of light by atoms in the gaseous state.
- First observed by Fraunhofer while studying dark lines in the solar spectrum.
- Developed by Alan Walsh in 1955, who applied the principle to the analysis of chemical elements.
Filter (Monochromator)
- Made up of colored glass or a filter that selects one single wavelength of light.
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