Biomedical Laboratory Workflow Quiz
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Questions and Answers

What should an ideal laboratory consist of?

  • Two pre-amplification rooms (correct)
  • One post-amplification room
  • One sample preparation room
  • One pre-amplification room

What should never be present in the reagent preparation room?

  • Reagent stock
  • Amplification products
  • Mechanical barriers
  • Extracted DNA/RNA or PCR products (correct)

What is the purpose of using color-coded lab coats in different spatially separated areas?

  • To indicate the temperature of the area
  • To enhance the visual appeal of the laboratory
  • To identify different team members
  • To prevent contamination (correct)

What should the pre-amplification rooms be under?

<p>Positive air pressure (C)</p> Signup and view all the answers

What is the primary responsibility of the molecular biology laboratory?

<p>Development and performance of molecular testing (B)</p> Signup and view all the answers

Which technique is NOT used in the molecular biology laboratory?

<p>Microarray analysis (B)</p> Signup and view all the answers

What is the purpose of mechanical barriers in the molecular biology laboratory?

<p>To prevent contamination and carryover of amplification products (D)</p> Signup and view all the answers

What is the purpose of a vortex mixer?

<p>Mixing small vials quickly in an oscillating circular motion and for the resuspension of cells (A)</p> Signup and view all the answers

Which factor is NOT considered in the design of laboratory space for molecular biology?

<p>Proximity to other laboratories (A)</p> Signup and view all the answers

What is the purpose of a centrifuge?

<p>Separating fluids, gases, or liquids based on density (C)</p> Signup and view all the answers

What is the purpose of a spectrophotometer?

<p>Measuring light absorption in a sample to determine nucleic acid quantitation or the number of chemicals in a solution (A)</p> Signup and view all the answers

What is the purpose of an incubator?

<p>Maintaining optimal conditions, such as temperature and humidity (D)</p> Signup and view all the answers

What is the purpose of equipping each area with disposable devices, laboratory coats, gloves, and ventilation systems?

<p>To impede anything coming out of the laboratory and contaminating the surrounding environment (C)</p> Signup and view all the answers

Why should the 'Dirty room' be under negative air pressure?

<p>To impede anything coming out of the laboratory and contaminating the surrounding environment (C)</p> Signup and view all the answers

Which area is referred to as the 'PCR room'?

<p>Amplification and post-amplification analysis room (A)</p> Signup and view all the answers

What is the main function of the amplification and post-amplification analysis room?

<p>To perform in vitro amplification steps and analysis of the amplified material (A)</p> Signup and view all the answers

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