Biomedical Laboratory Workflow Quiz

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Questions and Answers

What should an ideal laboratory consist of?

Two pre-amplification rooms (correct)
One post-amplification room
One sample preparation room
One pre-amplification room

What should never be present in the reagent preparation room?

Reagent stock
Amplification products
Mechanical barriers
Extracted DNA/RNA or PCR products (correct)

What is the purpose of using color-coded lab coats in different spatially separated areas?

To indicate the temperature of the area
To enhance the visual appeal of the laboratory
To identify different team members
To prevent contamination (correct)

What should the pre-amplification rooms be under?

Positive air pressure (C) Signup and view all the answers

What is the primary responsibility of the molecular biology laboratory?

Development and performance of molecular testing (B) Signup and view all the answers

Which technique is NOT used in the molecular biology laboratory?

Microarray analysis (B) Signup and view all the answers

What is the purpose of mechanical barriers in the molecular biology laboratory?

To prevent contamination and carryover of amplification products (D) Signup and view all the answers

What is the purpose of a vortex mixer?

Mixing small vials quickly in an oscillating circular motion and for the resuspension of cells (A) Signup and view all the answers

Which factor is NOT considered in the design of laboratory space for molecular biology?

Proximity to other laboratories (A) Signup and view all the answers

What is the purpose of a centrifuge?

Separating fluids, gases, or liquids based on density (C) Signup and view all the answers

What is the purpose of a spectrophotometer?

Measuring light absorption in a sample to determine nucleic acid quantitation or the number of chemicals in a solution (A) Signup and view all the answers

What is the purpose of an incubator?

Maintaining optimal conditions, such as temperature and humidity (D) Signup and view all the answers

What is the purpose of equipping each area with disposable devices, laboratory coats, gloves, and ventilation systems?

To impede anything coming out of the laboratory and contaminating the surrounding environment (C) Signup and view all the answers

Why should the 'Dirty room' be under negative air pressure?

To impede anything coming out of the laboratory and contaminating the surrounding environment (C) Signup and view all the answers

Which area is referred to as the 'PCR room'?

Amplification and post-amplification analysis room (A) Signup and view all the answers

What is the main function of the amplification and post-amplification analysis room?

To perform in vitro amplification steps and analysis of the amplified material (A) Signup and view all the answers

Flashcards

Ideal laboratory setup

Two pre-amplification rooms.

Reagent preparation room restrictions

Extracted DNA/RNA or PCR products.