16 Questions
What should an ideal laboratory consist of?
Two pre-amplification rooms
What should never be present in the reagent preparation room?
Extracted DNA/RNA or PCR products
What is the purpose of using color-coded lab coats in different spatially separated areas?
To prevent contamination
What should the pre-amplification rooms be under?
Positive air pressure
What is the primary responsibility of the molecular biology laboratory?
Development and performance of molecular testing
Which technique is NOT used in the molecular biology laboratory?
Microarray analysis
What is the purpose of mechanical barriers in the molecular biology laboratory?
To prevent contamination and carryover of amplification products
What is the purpose of a vortex mixer?
Mixing small vials quickly in an oscillating circular motion and for the resuspension of cells
Which factor is NOT considered in the design of laboratory space for molecular biology?
Proximity to other laboratories
What is the purpose of a centrifuge?
Separating fluids, gases, or liquids based on density
What is the purpose of a spectrophotometer?
Measuring light absorption in a sample to determine nucleic acid quantitation or the number of chemicals in a solution
What is the purpose of an incubator?
Maintaining optimal conditions, such as temperature and humidity
What is the purpose of equipping each area with disposable devices, laboratory coats, gloves, and ventilation systems?
To impede anything coming out of the laboratory and contaminating the surrounding environment
Why should the 'Dirty room' be under negative air pressure?
To impede anything coming out of the laboratory and contaminating the surrounding environment
Which area is referred to as the 'PCR room'?
Amplification and post-amplification analysis room
What is the main function of the amplification and post-amplification analysis room?
To perform in vitro amplification steps and analysis of the amplified material
Test your knowledge of biomedical laboratory workflow and safety measures with this quiz. Questions cover unidirectional traffic flow, necessary instruments, disposable devices, and good laboratory habits.
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